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Volume 10
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10.3390/cells10082088
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Protocol

Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies

by
Florian Hoppel
1,2,
Luiz Felipe Garcia-Souza
1,3,
Wilhelm Kantner-Rumplmair
4,
Martin Burtscher
2,
Erich Gnaiger
1,3,
Dominik Pesta
2,5,6,7,8,9,* and
Elisa Calabria
10,*
1
Oroboros Instruments, 6020 Innsbruck, Austria
2
Department of Sport Science, University of Innsbruck, 6020 Innsbruck, Austria
3
D. Swarovski Research Laboratory, Department of Visceral, Transplant Thoracic Surgery, Medical University of Innsbruck, 6020 Innsbruck, Austria
4
Psychosomatic Pain Ambulance, University Hospital for Medical Psychology and Psychotherapy, 6020 Innsbruck, Austria
5
German Aerospace Center (DLR), Institute of Aerospace Medicine, 51147 Cologne, Germany
6
Institute for Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University, 40225 Düsseldorf, Germany
7
German Center for Diabetes Research (DZD e.V.), 85764 München-Neuherberg, Germany
8
Centre for Endocrinology, Diabetes and Preventive Medicine (CEDP), University Hospital Cologne, 50924 Cologne, Germany
9
Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), 50931 Cologne, Germany
10
Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37131 Verona, Italy
*
Authors to whom correspondence should be addressed.
Cells 2021, 10(8), 2088; https://doi.org/10.3390/cells10082088
Submission received: 10 June 2021 / Revised: 24 July 2021 / Accepted: 6 August 2021 / Published: 14 August 2021
(This article belongs to the Section Mitochondria)
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Abstract

Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field studies, using high-resolution respirometry (HRR). Several factors may trigger PLT aggregation during the assay, altering the homogeneity of the cell suspension and distorting the number of cells added to the two chambers (A, B) of the Oroboros Oxygraph-2k (O2k). Thus, inter-chamber variability (∆ab) was calculated by normalizing oxygen consumption to chamber volume (JO2) or to a specific respiratory control state (flux control ratio, FCR) as a reliable parameter of experimental quality. The method’s reliability was tested by comparing the ∆ab of laboratory-performed experiments (LAB, N = 9) to those of an ultramarathon field study (three sampling time-points: before competition (PRE, N = 7), immediately after (POST, N = 10) and 24 h after competition (REC; N = 10)). Our results show that ∆ab JO2 changed PRE-POST, but also for LAB-POST and LAB-REC, while all ∆ab FCR remained unchanged. Thus, we conclude that our method is reliable for assessing PLT mitochondrial function in LAB and field studies and after systemic stress conditions.
Keywords: platelets; respirometry; field study; physical exercise; quality control; mitochondrial function platelets; respirometry; field study; physical exercise; quality control; mitochondrial function

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MDPI and ACS Style

Hoppel, F.; Garcia-Souza, L.F.; Kantner-Rumplmair, W.; Burtscher, M.; Gnaiger, E.; Pesta, D.; Calabria, E. Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies. Cells 2021, 10, 2088. https://doi.org/10.3390/cells10082088

AMA Style

Hoppel F, Garcia-Souza LF, Kantner-Rumplmair W, Burtscher M, Gnaiger E, Pesta D, Calabria E. Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies. Cells. 2021; 10(8):2088. https://doi.org/10.3390/cells10082088

Chicago/Turabian Style

Hoppel, Florian, Luiz Felipe Garcia-Souza, Wilhelm Kantner-Rumplmair, Martin Burtscher, Erich Gnaiger, Dominik Pesta, and Elisa Calabria. 2021. "Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies" Cells 10, no. 8: 2088. https://doi.org/10.3390/cells10082088

APA Style

Hoppel, F., Garcia-Souza, L. F., Kantner-Rumplmair, W., Burtscher, M., Gnaiger, E., Pesta, D., & Calabria, E. (2021). Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies. Cells, 10(8), 2088. https://doi.org/10.3390/cells10082088

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