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Artificial Induction of Spawning in Threeline Grunt, Parapristipoma trilineatum Under Controlled Environmental Conditions
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Evaluation of Fish Biodiversity in Estuaries Through Environmental DNA Metabarcoding: A Comprehensive Review
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Comparing Chemoautotrophic and Heterotrophic Biofloc Systems for Integrated Cultivation of Shrimp and Tilapia
Journal Description
Fishes
Fishes
is an international, peer-reviewed, scientific, open access journal published monthly online by MDPI. It covers fishes and aquatic animals research. The Iberian Society of Ichthyology (SIBIC) and the Brazilian Society of Aquaculture and Aquatic Biology (Aquabio) are affiliated with Fishes and their members receive a discount on the article processing charges.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubAg, FSTA, and other databases.
- Journal Rank: JCR - Q2 (Marine and Freshwater Biology)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 17.4 days after submission; acceptance to publication is undertaken in 2.6 days (median values for papers published in this journal in the second half of 2024).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
- Testimonials: See what our editors and authors say about Fishes.
- Companion Journal: Aquaculture Journal
Impact Factor:
2.1 (2023);
5-Year Impact Factor:
2.4 (2023)
Latest Articles
Assessment of Exploited Stock and Management Implications of Tiger Tooth Croaker (Otolithes ruber) in Coastal Waters of Makran, Pakistan
Fishes 2025, 10(5), 238; https://doi.org/10.3390/fishes10050238 - 20 May 2025
Abstract
Pakistan’s marine fishing industry is crucial to the country’s economy, generating employment opportunities and foreign revenue. It produces 80% of the country’s total fish production. Otolithes ruber is a commercially important fish on the Makran coast of Pakistan, contributing significantly to the region’s
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Pakistan’s marine fishing industry is crucial to the country’s economy, generating employment opportunities and foreign revenue. It produces 80% of the country’s total fish production. Otolithes ruber is a commercially important fish on the Makran coast of Pakistan, contributing significantly to the region’s croaker fisheries. This study is the first to apply three length-based approaches for assessing the stock status of O. ruber in the Makran coast: (1) TropFishR to estimate the mortality, growth parameters, and current exploitation status, reference points based on the yield per recruitment model, (2) the length-based Bayesian biomass method (LBB) to calculate stock biomass, and (3) the length-based spawning potential ratio (LBSPR) to estimate the spawning potential ratio. The length–weight relationship of Otolithes ruber was a positive allometric pattern (b = 3.28; R2 = 0.94). Growth parameters for Otolithes ruber were L∞ = 55.47 cm, K = 0.50 year−1. The calculated total mortality rate (Z), natural mortality (M), and fishing mortality (F) were 2.27 year−1, 0.67 year−1, and 1.6 year−1, respectively. The exploitation rate (E) was 0.70, indicating severe overexploitation. The current length at first capture (Lc50) = 27.37 cm was lower than that at first maturity (Lm50) = 30.75 cm, indicating growth overfishing. The current spawning potential ratio (8%) was lower than the optimal value (40%), indicating recruitment overfishing. The current biomass, concerning virgin biomass B/Bo, was also 8%, resulting in a 92% stock decline. We recommend reducing the exploitation pressure by limiting the commercial catch to an optimum length range of 34.5–42.2 cm and reducing fishing pressure by 40% to ensure sustainable fishery management.
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(This article belongs to the Special Issue The Roles of Fishery Biology and Fish Population Dynamics in Fisheries Management)
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Open AccessArticle
Citizen Science Illuminates a City-Dwelling Whale: A Report on the Large Aggregation of Narrow-Ridged Finless Porpoises in Tokyo Bay, Japan
by
Gen Nakamura and Ayumi Hirose
Fishes 2025, 10(5), 237; https://doi.org/10.3390/fishes10050237 - 19 May 2025
Abstract
Tokyo Bay is one of the busiest ocean areas for human activity worldwide, characterized by a high density of maritime traffic and industrial development. This area is also recognized as the habitat of the narrow-ridged finless porpoise, an endangered toothed whale. Although the
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Tokyo Bay is one of the busiest ocean areas for human activity worldwide, characterized by a high density of maritime traffic and industrial development. This area is also recognized as the habitat of the narrow-ridged finless porpoise, an endangered toothed whale. Although the existence of this species has been recognized, its scientific data, such as population size and geographic distribution, are limited in the region. Therefore, using social media, we initiated a research project to collect sighting information on this species in Tokyo Bay. As a result, 27 valid pieces of information on the finless porpoises were collected from July 2024 to the end of February 2025. The sightings were reported mainly in the waters north of Futtsu Cape, indicating that this species is localized in the northern part of Tokyo Bay. Also, most of the reported sightings were of groups of wo or more animals. Notably, a school of at least 30 animals was recorded, marking the most prominent school ever observed in the region and the third largest in Japan. This study highlights the importance of the northern region of Tokyo Bay as a habitat for this species. The effective integration of citizen science with scientific and quantitative research will enhance our understanding of the life history of this endangered species, thereby supporting conservation efforts.
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(This article belongs to the Section Biology and Ecology)
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Open AccessArticle
Aquaculture Areas Extraction Model Using Semantic Segmentation from Remote Sensing Images at the Maowei Sea of Beibu Gulf
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Weirong Qin, Mohd Hasmadi Ismail, Yangyang Luo, Yifeng Yuan, Junlin Deng, Mohammad Firuz Ramli and Ning Wu
Fishes 2025, 10(5), 236; https://doi.org/10.3390/fishes10050236 - 19 May 2025
Abstract
The extraction of aquaculture areas from high-resolution remote sensing images is crucial for effective coastal management and resource preservation. This study introduces SwinNet, a semantic segmentation model leveraging multi-scale feature fusion to enhance the extraction of aquaculture areas, particularly in the Maowei Sea
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The extraction of aquaculture areas from high-resolution remote sensing images is crucial for effective coastal management and resource preservation. This study introduces SwinNet, a semantic segmentation model leveraging multi-scale feature fusion to enhance the extraction of aquaculture areas, particularly in the Maowei Sea of the Beibu Gulf, China. Utilizing the Swin Transformer backbone and a novel Parallel Pooling Attention Module (PPAM), SwinNet minimizes background noise and improves segmentation accuracy. SwinNet achieved a pixel accuracy of 96.53% and an intersection over the union of 93.07% on an aquaculture dataset, demonstrating superior performance in overcoming noise and accurately extracting aquaculture areas. SwinNet offers an effective solution for large-scale, high-precision monitoring of coastal aquaculture, with potential broader applicability in aquatic resource conservation and management.
Full article
(This article belongs to the Special Issue Machine Learning in Aquaculture)
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Open AccessReview
A Review of Standardization in Mississippi’s Multidecadal Inland Fisheries Monitoring Program
by
Caleb A. Aldridge and Michael E. Colvin
Fishes 2025, 10(5), 235; https://doi.org/10.3390/fishes10050235 - 18 May 2025
Abstract
Standardizing data collection, management, and analysis processes can improve the reliability and efficiency of fisheries monitoring programs, yet few studies have examined the operationalization of these tasks within agency settings. We reviewed the Mississippi Department of Wildlife, Fisheries, and Parks, Fisheries Bureau’s inland
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Standardizing data collection, management, and analysis processes can improve the reliability and efficiency of fisheries monitoring programs, yet few studies have examined the operationalization of these tasks within agency settings. We reviewed the Mississippi Department of Wildlife, Fisheries, and Parks, Fisheries Bureau’s inland recreational fisheries monitoring program—a 30+-year effort to standardize field protocols, data handling procedures, and automated analyses through a custom-built computer application, the Fisheries Resources Analysis System (FRAS). Drawing on quantitative summaries of sampling trends and qualitative interviews with fisheries managers, we identified key benefits, challenges, and opportunities associated with the Bureau’s standardization efforts. Standardized procedures improved sampling consistency, data reliability, and operational efficiency, enabling the long-term tracking of fish population and angler metrics across more than 270 managed waterbodies. However, challenges related to analytical transparency and spatiotemporal comparisons persist. Simulations indicated that under current conditions, 5.8, 22.9, and 37.1 years would be required to sample (boat electrofishing) 50%, 75%, and 95% of the Bureau’s waterbodies at least once, respectively; these figures should translate to other agencies, assuming similar resource availability per waterbody. The monitoring program has reduced manual processing effort and enhanced staff capacity for waterbody-specific management, yet several opportunities remain to improve efficiency and utility. These include expanding FRAS functionalities for trend visualization, integrating mobile field data entry to reduce transcription errors, linking monitoring results with management objectives, and enhancing automated report generation for management support. Strengthening these elements could not only streamline workflows but better position agencies to apply standardized data in adaptive management embedded into the monitoring program.
Full article
(This article belongs to the Section Fishery Economics, Policy, and Management)
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Open AccessArticle
Multi-Omics Investigation of Fatty Acid Content Variations in Common Carp (Cyprinus carpio) Muscle: Integrating Genome, Transcriptome, and Lipid Profiling Data
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Kaikuo Wang, Qi Wang, Yingjie Chen, Yiming Cao, Ran Zhao, Shuangting Yu, Xiaoqing Sun, Yan Zhang and Jiongtang Li
Fishes 2025, 10(5), 234; https://doi.org/10.3390/fishes10050234 - 16 May 2025
Abstract
Fatty acids (FAs), especially the polyunsaturated fatty acids (PUFAs), play pivotal roles in growth and development. Fish possess a remarkable ability to synthesize PUFAs, rendering them a crucial and significant source of these essential FAs for human consumption. Despite extensive research on the
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Fatty acids (FAs), especially the polyunsaturated fatty acids (PUFAs), play pivotal roles in growth and development. Fish possess a remarkable ability to synthesize PUFAs, rendering them a crucial and significant source of these essential FAs for human consumption. Despite extensive research on the metabolic processes of FAs, the primary processes underlying FA content variation and corresponding regulatory mechanisms remain largely unexplored. We quantified the muscular FA profiles of 304 common carp samples. High variation in muscle FA content among samples was observed, with the coefficients of variation ranging from 0.36 to 0.92. With genome-wide association studies (GWAS), we identified 1410 SNPs significantly associated with the FA content. The genetic variation at genes participating in oxidation, ubiquitination, and transcriptional factors was associated with the content of multiple FAs, while few variants were observed in the FA-synthesis genes. For the total PUFA (TPUFA) content, transcriptome analysis of groups with the highest and lowest TPUFA content identified 715 differentially expressed genes (DEGs), including genes involved in oxidation and ubiquitination. Using multiple genomic selection programs with the associated SNPs for FA content, we obtained high and positive correlations ranging from 0.65 to 0.92 between the real FA contents and estimated breeding values. These findings manifested the major-effect processes governing the differences in muscular FA content and their regulatory mechanisms. The optimal genomic selection programs provide novel and feasible perspectives to enhance FA content in common carp.
Full article
(This article belongs to the Special Issue Molecular Design Breeding in Aquaculture)
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Open AccessArticle
Fick’s Diffusion Laws and Scaling of the Gill Surface Area and Oxygen Uptake in Fish
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Daniel Pauly and Johannes Müller
Fishes 2025, 10(5), 233; https://doi.org/10.3390/fishes10050233 - 16 May 2025
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The oxygen consumption of adult fish (Q) is proportional to their body weight (W) raised to a power, estimated as the slope (dQ) of a linear regression of log(Q) vs. log(W). Similarly,
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The oxygen consumption of adult fish (Q) is proportional to their body weight (W) raised to a power, estimated as the slope (dQ) of a linear regression of log(Q) vs. log(W). Similarly, the gill surface area of adult fish (GSA) is proportional to W raised to the power dG, as also estimated via a log-log linear proportional to their surface area. Moreover, because of Fick’s laws of diffusion, dQ should be at least similar to dG. Recently, the claim has been made that non-zero differences between dQ and dG invalidate the Gill-Oxygen Limitation Theory (GOLT), which suggests that the O2 supplied by gills growing with dG <1 limits the growth of older and larger fish. We show here, based on 33 pairs of dG and dQ in 33 fish species and other information that (i) while individual differences between dQ and dG are observed in several cases, there is no significant overall difference across the 33 estimates and (ii) large differences between dQ and dG are primarily due to ontogenetic changes in scaling (OCS), likely ontogenetic changes in gills’ water–blood (or ‘diffusion’) distance and, as well, multiple sources of experimental variability and potential errors, leading to outliers and random differences.
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Open AccessArticle
Model Selection Applied to Growth of the Stingray Urotrygon chilensis (Günther, 1872) in the Southeastern Mexican Pacific
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Ana Bricia Guzmán-Castellanos, Enrique Morales-Bojórquez, Hugo Aguirre-Villaseñor and Javier Tovar-Ávila
Fishes 2025, 10(5), 232; https://doi.org/10.3390/fishes10050232 - 16 May 2025
Abstract
The present study analyzed the growth pattern of the stingray Urotrygon chilensis caught as bycatch by the shrimp fishery in the southeastern Mexican Pacific. From January to December 2012, the thoracic vertebrae of 491 females and 205 males were collected. Female ages ranged
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The present study analyzed the growth pattern of the stingray Urotrygon chilensis caught as bycatch by the shrimp fishery in the southeastern Mexican Pacific. From January to December 2012, the thoracic vertebrae of 491 females and 205 males were collected. Female ages ranged from 0 to 14 years, whereas male ages ranged from 0 to 12 years. The marginal increment and edge analyses suggested the annual formation of growth bands in the vertebrae. The size-at-age data were analyzed using the multimodel inference approach; six candidate growth models were compared, including models with a theoretical age-at-zero total length, mean size-at-birth, and generalized models. Based on Akaike’s information criterion, the best statistical fit to the size-at-age data was the two-phase Gompertz growth model (k = −0.13, G = 1.59, = 10.40) for males and the two-parameter Gompertz growth model (k = 1.42, α = 0.15, = 10.90) for females. In this study, we compare the growth parameters among batoid species, finding that U. chilensis has a relatively short lifespan, slower growth, and that females are larger than males.
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(This article belongs to the Section Biology and Ecology)
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Open AccessArticle
The Effect of Ontogenetic Dietary Shifts on the Trophic Structure of Fish Communities Based on the Trophic Spectrum
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Junwei Xu, Jie Yin, Binduo Xu, Chongliang Zhang, Yupeng Ji, Yiping Ren and Ying Xue
Fishes 2025, 10(5), 231; https://doi.org/10.3390/fishes10050231 - 15 May 2025
Abstract
The trophic spectrum provides a useful method to investigate the trophic structure of fish communities. However, ontogenetic shifts in diet can cause variations in the trophic level with body size, thereby influencing the trophic structure of fish communities. In this study, we investigated
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The trophic spectrum provides a useful method to investigate the trophic structure of fish communities. However, ontogenetic shifts in diet can cause variations in the trophic level with body size, thereby influencing the trophic structure of fish communities. In this study, we investigated the effect of ontogenetic dietary shifts on the trophic structure of fish communities in Haizhou Bay based on trophic spectra and trophic indicators calculated under different scenarios of functional group classification. The results showed that the size threshold of ontogenetic dietary shifts was a viable basis for functional group classification. The biomass of fishes at trophic levels 3.4–3.8 was lower when ontogenetic dietary shifts were considered, which can influence the intensity of top–down control and hinder the formulation of harvest strategies. Ontogenetic dietary shifts can also redistribute the biomass of fishes across trophic levels, thereby regulating the trophic structure of fish communities. Different responses of trophic indicators to ontogenetic dietary shifts were identified, with high trophic indicator (HTI) being the most appropriate indicator reflecting the effect of ontogenetic dietary shifts in the trophic structure. This study provides a feasible way to optimize the trophic spectrum for elucidating the trophic structure of fish communities. And we recommend that ontogenetic dietary shifts should be considered and valued in fishery management.
Full article
(This article belongs to the Special Issue Trophic Ecology of Freshwater and Marine Fish Species)
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Assessing Diadromous Fish Populations in the Lima River, Northwest Iberian Peninsula
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Luís Pereira, Ulisses Azeiteiro and Carlos Antunes
Fishes 2025, 10(5), 230; https://doi.org/10.3390/fishes10050230 - 15 May 2025
Abstract
The Lima River in northern Portugal serves as a vital habitat for diadromous fish species, yet it remains one of the least studied aquatic systems in the region. This study investigates the ecology and biology of key diadromous fish populations, including sea lamprey
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The Lima River in northern Portugal serves as a vital habitat for diadromous fish species, yet it remains one of the least studied aquatic systems in the region. This study investigates the ecology and biology of key diadromous fish populations, including sea lamprey (Petromyzon marinus), shads (Alosa alosa and Alosa fallax), trout (Salmo trutta), and European eel (Anguilla anguilla), during their reproductive migration and riverine periods. A total of 3242 individuals from 15 species were sampled, with native species accounting for 51.1% of the catch. Results revealed significant differences in size and weight of lampreys, with individuals from the Lima River being significantly smaller than those from regional rivers such as the Minho and Mondego. Shad populations showed a high proportion of hybrids (33.8%), suggesting extensive hybridization between A. alosa and A. fallax. Analysis of trout stomach contents revealed a diverse diet dominated by insects (66.9%), crustaceans (6.8%), and fish (6.8%), but also an alarming presence of plastic debris (1.1%), highlighting potential pollution problems. For European eels, this study marks the first record of the invasive swim bladder parasite Anguillicola crassus in the Lima River, with 84.8% of eels sampled showing moderate to severe swim bladder damage. These findings contribute to a deeper understanding of diadromous fish ecology and emphasize the importance of conservation efforts in estuarine systems worldwide.
Full article
(This article belongs to the Section Biology and Ecology)
Open AccessArticle
Effects of Clozapine, Haloperidol, and the NMDA Antagonist Ketamine on Novel Object Recognition in Gnathonemus petersii: A New Possible Model for Schizophrenia Research
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Petra Horká, Josefina Mavrogeni, Veronika Langová, Pavel Horký, Jan Hubený, Ivana Chrtková, Karel Valeš, Martin Kuchař and Jiří Horáček
Fishes 2025, 10(5), 229; https://doi.org/10.3390/fishes10050229 - 15 May 2025
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In animal models, ketamine, a non-competitive N-methyl-D-aspartate (NMDA) antagonist, induces schizophrenia-like symptoms, such as positive and negative symptoms, as well as cognitive deficits. In the present study, we evaluated the behavioral responses and the number of EODs (electric organ discharges) of the weakly
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In animal models, ketamine, a non-competitive N-methyl-D-aspartate (NMDA) antagonist, induces schizophrenia-like symptoms, such as positive and negative symptoms, as well as cognitive deficits. In the present study, we evaluated the behavioral responses and the number of EODs (electric organ discharges) of the weakly electric fish Gnathonemus petersii using the novel object recognition task (NORT). We aimed to investigate whether pharmacological modulation of the glutamatergic system would impair cognitive functions by administering the NMDA receptor antagonist ketamine, and whether these impairments could be suppressed by the administration of typical (first-generation) and atypical (second-generation) antipsychotics—clozapine and haloperidol, respectively. G. petersii preferred the familiar object over the novel object in the NORT paradigm. Although no significant differences were observed when exploring the two identical objects during the training session, the fish spent less time, moved a shorter distance, and emitted fewer EODs in the testing phase with the novel object. No direct relationship was detected between the EODs and behavioral responses to the administration of ketamine and typical antipsychotics. Ketamine administered with atypical antipsychotic clozapine disrupted the perception of the original object, where one of the objects was preferred. In the novel object trial, the time spent on the original and new objects was attenuated to the same level.
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Open AccessArticle
Effects of Increased Feeding Rates on Oxidative Stress, Biochemical Indices and Growth of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus tukula ♂) Under Mild-Hyperoxia Conditions
by
Zhiyi Wang, Yikai Zheng, Dengpan Dong, Xiefa Song and Meng Li
Fishes 2025, 10(5), 228; https://doi.org/10.3390/fishes10050228 - 15 May 2025
Abstract
Evaluating the interaction between dissolved oxygen (DO) and feeding rates (FRs) in fish is crucial for the precise regulation of aquaculture water environments. This study established four treatment groups: the CK group (DO = 6 mg/L, FR = 2% of body weight), the
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Evaluating the interaction between dissolved oxygen (DO) and feeding rates (FRs) in fish is crucial for the precise regulation of aquaculture water environments. This study established four treatment groups: the CK group (DO = 6 mg/L, FR = 2% of body weight), the HFR group (DO = 6 mg/L, FR = 3.5% of body weight), the HDO group (DO = 9 mg/L, FR = 2% of body weight), and the MIX group (DO = 6 mg/L, FR = 3.5% of body weight). The combined effects of dissolved oxygen and feeding levels on oxidative stress, biochemical indicators, and growth in the hybrid grouper were evaluated. The results showed that mild hyperoxia significantly upregulates the expression of antioxidant enzyme genes (cat, cu/zn-sod, and gpx1a). Under conditions of mild hyperoxia, an increased feed rate can significantly downregulate the expression of cat and gpx1a. Additionally, serum levels of carnosine and cndp1 in muscle tissue are significantly elevated. Furthermore, a high FR mitigates the downregulation of glucose, triglycerides, and alanine aminotransferase (ALT) induced by mild hyperoxia while alleviating the upregulation of aspartate aminotransferase (AST). The combined effects of mild hyperoxia and high FR significantly enhance final body weight and specific growth rate (SGR), with notable interactions observed. Mild hyperoxia reduces serum levels of bile acids and glycocholic acid under high feeding conditions while significantly downregulating the expression of ghrb in both liver and brain tissues. In summary, high FRs alleviate oxidative stress and energy substrate deficiency in juvenile hybrid grouper under mild-hyperoxia environments. Moreover, the synergistic effect between mild hyperoxia and high FR promotes growth by improving bile acid enterohepatic circulation. This study provides a reference for the regulation of DO and feeding in modern industrial intensive mariculture.
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(This article belongs to the Section Physiology and Biochemistry)
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Open AccessTechnical Note
Application of Flow Cytometry to Determine Cell DNA Content in the Genetic Breeding of Fish
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Xinyan Zhu, Yang Chen, Xiaodie Zhang, Jiaxu Qiang, Lingtao Nie, Xinyue Luo, Binchao Liang, Kuo Chen, Fuzhong Yang, Rurong Zhao and Chun Zhang
Fishes 2025, 10(5), 227; https://doi.org/10.3390/fishes10050227 - 15 May 2025
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In the field of fish genetic breeding, accurately determining the DNA content and ploidy of fish is of great significance. This article introduces the use of flow cytometry (FCM) to measure the DNA content and conduct ploidy analysis by sampling different tissues of
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In the field of fish genetic breeding, accurately determining the DNA content and ploidy of fish is of great significance. This article introduces the use of flow cytometry (FCM) to measure the DNA content and conduct ploidy analysis by sampling different tissues of freshwater fish species. It describes the FCM detection methods and their effectiveness for different individual tissues. These tissues include embryos and fry, as well as the blood, caudal fins, and sperm of adult live fish, and also specific tissues such as testes, ovaries, gills, spleens, and livers under anatomical conditions. Moreover, the application of ploidy detection to different tissues or individuals in different stages in the practice of fish genetic breeding is analyzed. This research covers samples from different growth stages and a variety of tissue types. The results show that this method exhibits high stability and reliability in the detection of different tissue samples, providing solid data support for subsequent research. It holds significant application value in fish genetic breeding.
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Open AccessArticle
Effect of Yeast Selenium on Growth Performance, Muscle Selenium Deposition, and Antioxidant Capacity of Juvenile Cherax quadricarinatus
by
Ying Han, Chenchen Wang, Jimin Deng, Lizhen Zhong, Xiao Huang, Yuandong Sun and Xiaojuan Cui
Fishes 2025, 10(5), 226; https://doi.org/10.3390/fishes10050226 - 15 May 2025
Abstract
This study aimed to investigate the effects of organic selenium-enriched yeast on the growth performance, muscle selenium deposition, and antioxidant capacity of juvenile Cherax quadricarinatus. Five experimental diets supplemented with 0.00 (control), 1.00, 2.00, 3.00, and 4.00 mg/kg of selenium-enriched yeast were
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This study aimed to investigate the effects of organic selenium-enriched yeast on the growth performance, muscle selenium deposition, and antioxidant capacity of juvenile Cherax quadricarinatus. Five experimental diets supplemented with 0.00 (control), 1.00, 2.00, 3.00, and 4.00 mg/kg of selenium-enriched yeast were fed to crayfish with an average initial body weight of (6.35 ± 1.66) g for 56 days. On days 0, 14, 28, 42, and 56 of the trial, 10 crayfish from each group were randomly sampled for body weight measurement. On day 28, the body weight of the crayfish fed diets containing 1.00, 2.00, 3.00, and 4.00 mg/kg selenium-enriched yeast was significantly higher (p < 0.05) than that of the control group. On day 56, the weight gain rate (WGR) and specific growth rate (SGR) of crayfish fed 1.00, 2.00, and 3.00 mg/kg selenium-enriched yeast were significantly elevated (p < 0.05) compared to those of the control group. Dietary selenium supplementation significantly enhanced muscle selenium deposition (p < 0.05), with deposition levels increasing proportionally to the supplementation levels within the same period. Selenium deposition initially increased, peaked at day 28 (significantly higher than the control), and then declined and stabilized. On day 42, the activities of pancreatic lipase (LPS), total superoxide dismutase (T-SOD), and acid phosphatase (ACP) in the hepatopancreas were significantly higher in the 3.00 and 4.00 mg/kg groups (p < 0.05). In comparison, trypsin activity was significantly increased in the 2.00 mg/kg and 3.00 mg/kg yeast selenium groups (p < 0.05). Alkaline phosphatase (AKP) activity was highly significantly elevated in the 2.00 mg/kg group (p < 0.01). On day 56, glutathione peroxidase (GSH-PX) activity in the hepatopancreas was considerably enhanced in all the selenium-supplemented groups (1.00–4.00) mg/kg (p < 0.05). In conclusion, the appropriate supplementation of selenium-enriched yeast promotes growth performance, enhances muscle selenium deposition, improves hepatopancreatic digestive enzyme activity, and strengthens antioxidant and immune capacities in Cherax quadricarinatus.
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(This article belongs to the Section Nutrition and Feeding)
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Open AccessArticle
Taurine Enhances Antioxidant Enzyme Activity and Immune Response in Seriola rivoliana Juveniles After Lipopolysaccharide Injection
by
Andressa Teles, Laura Guzmán-Villanueva, Marco A. Hernández-de Dios, Daniela A. Corona-Rojas, Minerva Maldonado-García and Dariel Tovar-Ramírez
Fishes 2025, 10(5), 225; https://doi.org/10.3390/fishes10050225 - 14 May 2025
Abstract
Additives in fish feeds are used worldwide to provide better productivity and improve fish’s health in facing disease outbreaks. This study aimed to identify the protective effect of taurine on the immune-related parameters and antioxidant enzyme activities of Seriola rivoliana juveniles after being
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Additives in fish feeds are used worldwide to provide better productivity and improve fish’s health in facing disease outbreaks. This study aimed to identify the protective effect of taurine on the immune-related parameters and antioxidant enzyme activities of Seriola rivoliana juveniles after being challenged with LPS (lipopolysaccharide). Previously, the fish were submitted to a feeding trial for 60 days with feed enriched with different doses of external taurine (0, 1%, and 2%). Juveniles fed on different doses of taurine were injected with LPS (0%, LPS+T0%; 1%, LPS+T1%; 2%, LPS+T2%), and a control group was injected with saline solution (LPS-). The immune-related mRNA expression was evaluated, as were lysozyme enzyme activity and antioxidant enzyme activities (superoxide dismutase—SOD; catalase). Regarding immune mRNA expression, all the treatments had a peak of expression at 24 h post-LPS-injection, with a sharp decrease at 72 h post-injection, reaching similar mRNA expression as at 0 h post-injection. The results showed that the LPS+T2% treatment improved the expression of il1-β, tnf-α, and tlr-3 at 24 h post-LPS injection. Antioxidant and lysozyme activities were higher in both treatments with taurine when compared to the LPS+T0% and LPS- groups after 72 h post-LPS injection. These results suggest using 2% of exogenous external taurine can improve immunocompetency and counteract the oxidative stress caused by exposure to LPS in S. rivoliana juveniles.
Full article
(This article belongs to the Special Issue Impacts of Dietary Supplements on Fish Growth and Health)
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Open AccessReview
Fish Welfare in the Ornamental Trade: Stress Factors, Legislation, and Emerging Initiatives
by
Caroline Marques Maia, Ana Carolina dos Santos Gauy and Eliane Gonçalves-de-Freitas
Fishes 2025, 10(5), 224; https://doi.org/10.3390/fishes10050224 - 13 May 2025
Abstract
The ornamental fish trade involves multiple phases, from acquisition—whether through wild capture or aquaculture—to the final destination, often including long transport routes. Throughout this trade, fish encounter several stressors like handling, improper maintenance methods, high or inadequate stocking densities, poor water quality, and
[...] Read more.
The ornamental fish trade involves multiple phases, from acquisition—whether through wild capture or aquaculture—to the final destination, often including long transport routes. Throughout this trade, fish encounter several stressors like handling, improper maintenance methods, high or inadequate stocking densities, poor water quality, and mechanical disturbances during transport. The varying levels of care provided by aquarists also influence the long-term welfare of these animals, with many fish being treated more as ornaments rather than sentient beings. Worsening these issues, there is a lack of scientifically grounded guidance for hobbyists and caregivers, important gaps in legislation concerning fish welfare, and limited oversight in the ornamental trade. Nevertheless, some international organizations and grassroots projects have been working to promote responsible practices and disseminate educational resources. However, the need for efficient welfare standards, legislation, and comprehensive information for all stakeholders involved in this sector remains critical. Here, we synthesize the problems in the ornamental fish trade and suggest some solutions to reduce the impacts of management practices on the welfare of these fish.
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(This article belongs to the Section Welfare, Health and Disease)
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Open AccessReview
Assessing the Effects of Pesticides on Aquacultured Fish and Ecosystems: A Comprehensive Environmental Health Review
by
Emily Burch, Mohamed Ali Hussein, Manar Zaki, Lereen T. Kamal, Ghada Zaki, Tamer Shoeib, Mahmoud Dawood, Hani Sewilam and Anwar Abdelnaser
Fishes 2025, 10(5), 223; https://doi.org/10.3390/fishes10050223 - 13 May 2025
Abstract
Aquaculture has been rapidly growing during the past decade to accommodate the increasing need for seafood as a vital source of nutrients for human beings. The nutritional benefits of incorporating fish into one’s diet are paramount in promoting overall health, bolstering immunity and
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Aquaculture has been rapidly growing during the past decade to accommodate the increasing need for seafood as a vital source of nutrients for human beings. The nutritional benefits of incorporating fish into one’s diet are paramount in promoting overall health, bolstering immunity and warding off diseases. Nonetheless, farm-raised aquatic species are frequently subjected to elevated contamination levels due to pesticides, antibiotics, and heavy metals in the marine environment. Pesticides affect fish differently based on species, class, dosage, and exposure duration. They can induce histological damage or neurobehavioral changes by inhibiting acetylcholinesterase production. This can promote liver dysfunction, metabolism deregulation, oxidative stress, and hematological imbalances, impair immune responses, and adversely affect fish reproduction. Furthermore, pesticides negatively affect the nutritional composition of fish by reducing the total protein levels in muscle, liver, gills, and kidney tissues. They disrupt lipid metabolism, resulting in lipid accumulation in the liver and a decrease in polyunsaturated fatty acids. Additionally, pesticides interfere with metabolism by altering carbohydrate levels in the gills, muscles, and kidneys while decreasing glycogen storage in the liver. Pesticide exposure has been linked to severe health impacts in humans, such as non-communicable diseases, reproductive issues, cognitive dysfunction, and cancer. The current review comprehensively emphasizes the harmful effects of pesticides on fish and human health, urging the establishment of environmental monitoring programs and biomonitoring studies. It accentuates the need for risk assessment models to evaluate pesticide impacts on marine ecosystems and advocates for stricter safety standards and lower pesticide residue limits in aquaculture products.
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(This article belongs to the Section Welfare, Health and Disease)
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Open AccessArticle
Effects of Stocking Density on Phytoplankton Community and Water Quality in Polyculture Ponds of Tegillarca granosa and Litopenaeus vannamei
by
Jing He, Lin He, Zhihua Lin and Yongjian Xu
Fishes 2025, 10(5), 222; https://doi.org/10.3390/fishes10050222 - 12 May 2025
Abstract
This study investigated the effects of culture density on the phytoplankton communities in an integrated culture model of the blood clam Tegillarca granosa and Pacific white shrimp Litopenaeus vannamei. Two treatments were set up: L (L. vannamei 18 ind/m2,
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This study investigated the effects of culture density on the phytoplankton communities in an integrated culture model of the blood clam Tegillarca granosa and Pacific white shrimp Litopenaeus vannamei. Two treatments were set up: L (L. vannamei 18 ind/m2, T. granosa 33 ind/m2) and H (L. vannamei 36 ind/m2, T. granosa 66 ind/m2). The management methods were the same during the experimental period, and the feed intake was determined according to L. vannamei. The results showed that there were more than 38 species of phytoplankton in the ponds, belonging to six phyla and 28 genera; the diversity index of the phytoplankton was higher in the early stage of the experiment and lower in the later stage; the average biomass of phytoplankton was 21.74 × 104 ind/L; and the culture density had no significant effect on water quality. This study showed that a reasonable increase in culture density would not cause significant adverse effects on the environment. It is possible to increase the stocking density of shrimp with the mollusk without causing inadequate conditions for the phytoplankton community and water quality.
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(This article belongs to the Section Sustainable Aquaculture)
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Open AccessArticle
Core–Shell Magnetic Gold Nanoparticles with Chitosan Coating as a SERS Substrate: A Rapid Detection Strategy for Malachite Green Contamination in Aquatic Foods
by
Yihui Yang, Tao Huang, Sijia Hu, Hang Ye, Jiali Xing and Shengnan Zhan
Fishes 2025, 10(5), 221; https://doi.org/10.3390/fishes10050221 - 11 May 2025
Abstract
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In this study, we developed a novel SERS-active magnetic substrate (MBs@CS@AuNPs) for detecting malachite green (MG) in aquatic products, including shrimp, cod, and aquaculture water. The substrate combines chitosan-functionalized magnetic nanobeads with dense gold nanoparticles. It efficiently enriches MG through electrostatic and π–π
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In this study, we developed a novel SERS-active magnetic substrate (MBs@CS@AuNPs) for detecting malachite green (MG) in aquatic products, including shrimp, cod, and aquaculture water. The substrate combines chitosan-functionalized magnetic nanobeads with dense gold nanoparticles. It efficiently enriches MG through electrostatic and π–π interactions, generates high-density plasmonic hotspots for stable signals, and utilizes a superparamagnetic core to concentrate MG molecules. This design achieved an ultralow detection limit of 10−9 M for MG in aquaculture samples, with a linear range spanning from 10−3 to 10−10 M (R2 = 0.999). The substrate demonstrated superior performance in untreated, complex food matrices (e.g., shrimp, cod), outperforming conventional magnetic mass spectrometry systems that are prone to matrix interference. This work introduces an innovative approach for detecting harmful residues in food during environmental safety monitoring.
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Open AccessPerspective
Championing Line Breeding and Hybridization in Aquaculture to Safeguard Intellectual Property
by
Gen Hua Yue
Fishes 2025, 10(5), 220; https://doi.org/10.3390/fishes10050220 - 9 May 2025
Abstract
Line breeding and line hybridization are pivotal genetic strategies in aquaculture, enabling breeders to enhance traits such as growth rate, disease resistance, and environmental tolerance in species like tilapia and Asian seabass. Though resource-intensive, these techniques yield long-term benefits, including improved productivity and
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Line breeding and line hybridization are pivotal genetic strategies in aquaculture, enabling breeders to enhance traits such as growth rate, disease resistance, and environmental tolerance in species like tilapia and Asian seabass. Though resource-intensive, these techniques yield long-term benefits, including improved productivity and sustainability. Traditional intellectual property (IP) protections, such as patents, are challenging to enforce in aquaculture because the biological replication and unauthorized breeding of aquatic species make it difficult to track and control proprietary genetic materials. Line hybridization offers a biological safeguard by producing hybrids that are challenging to replicate without access to proprietary parent lines. This inherent IP protection empowers breeders to safeguard their innovations, fostering sustainable growth and profitability in the aquaculture industry.
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(This article belongs to the Section Genetics and Biotechnology)
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Open AccessArticle
Observations on the Biology and Fishery of the Marbled Spinefoot (Siganus rivulatus Forsskål & Niebuhr, 1775) in the Eastern Red Sea
by
Zahra Okba, Eyüp Mümtaz Tıraşın and Mark Dimech
Fishes 2025, 10(5), 219; https://doi.org/10.3390/fishes10050219 - 8 May 2025
Abstract
This study aims to enhance our understanding of the marbled spinefoot (Siganus rivulatus) population along the Red Sea coast of Saudi Arabia. It investigates whether the stock is subject to overfishing and tests the hypothesis that current fishing mortality exceeds sustainable
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This study aims to enhance our understanding of the marbled spinefoot (Siganus rivulatus) population along the Red Sea coast of Saudi Arabia. It investigates whether the stock is subject to overfishing and tests the hypothesis that current fishing mortality exceeds sustainable thresholds. A total of 6192 specimens were sampled during a comprehensive survey conducted from 2022 to 2024, utilizing a range of fishing methods, including handline, trap, gillnet, and demersal trawl fisheries. The sampled fish ranged in total length (TL) from 100 to 335 mm and in total weight (W) from 17 to 470 g. The length–weight relationship was W = 0.0175 × TL2.92. Growth parameters derived from the von Bertalanffy model were TL∞ = 43.5 cm, K = 1.12 year−1, and t0 = −0.18 year. The median size at first maturity was estimated at 14.83 cm TL for both sexes. Virtual Population Analysis revealed fishing mortality rates ranging from 0.01 year−1 to 0.89 year−1 across age classes 1 to 5 years, with exploitation levels of 0.6, 0.55, and 0.5 at ages 3, 4, and 5, respectively, indicating slight overfishing. The annual average catch of marbled spinefoot along the Saudi Arabian Red Sea coast was approximately 211 tonnes, contributing an estimated 1.8 million USD to the national economy. Maintaining the current fishing effort at sustainable levels is essential to ensure the long-term viability of this stock.
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(This article belongs to the Section Biology and Ecology)
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