To date, several independent methods and algorithms exist for exploiting constraint-based stoichiometric models to find metabolic engineering strategies that optimize microbial production performance. Optimization procedures based on metaheuristics f...
Flux Balance Analysis (FBA) is a constraint-based method that is commonly used to guide metabolites through restricting pathways that often involve conditions such as anaplerotic cycles like Calvin, reversible or irreversible reactions, and nodes whe...
Since occupants spend most of their time indoors, an energy-saving and comfortable indoor environment are particularly important. The differences in the metabolic rate of occupants make them have different requirements for their thermal environment....
Metabolism is generally required for cellular maintenance and for the generation of offspring under conditions that support growth. The rates, yields (efficiencies), adaptation time and robustness of metabolism are therefore key determinants of cellu...
Studies enabled by metabolic models of different species of microalgae have become significant since they allow us to understand changes in their metabolism and physiological stages. The most used method to study cell metabolism is FBA, which commonl...
For the first time, the pharmacokinetic (PK) profile of tryptophanol-derived isoindolinones, previously reported as p53 activators, was investigated. From the metabolites’ identification, performed by liquid chromatography coupled to high resol...
Background: Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) hold transformative potential for cardiovascular regenerative medicine, yet their clinical application is hindered by suboptimal mitochondrial maturation and metabolic ineffi...
Rural communities can be conceptualized as spatial organisms interconnected by optimized resource utilization systems. Investigating the efficient utilization of rural resources and spatial construction methods grounded in resource metabolism is a pi...
As tumor research has deepened, the deregulation of cellular metabolism has emerged as yet another recognized hallmark of cancer. Tumor cells adapt different biochemical pathways to support their rapid growth, proliferation, and invasion, resulting i...
Fibroblasts play a fundamental role in maintaining tissue architecture, regulating repair processes, and adapting to metabolic and inflammatory stress. Increasing evidence indicates that endogenous bioelectrical states contribute to gene expression r...
This work investigates the use of an intelligent and unobstructive sensing technique for maintaining vehicle cabin’s indoor air quality while simultaneously assessing the driver metabolic rate. CO2 accumulation patterns are of great interest be...
Lower limb exoskeletons have the potential to mitigate work-related musculoskeletal disorders; however, they often lack user-oriented control strategies. Human-in-the-loop (HITL) controls adapt an exoskeleton’s assistance in real time, to optim...
One of the challenging tasks in systems biology is to understand how molecular networks give rise to emergent functionality and whether universal design principles apply to molecular networks. To achieve this, the biophysical, evolutionary and physio...
The goal-oriented control policies of cybernetic models have been used to predict metabolic phenomena such as the behavior of gene knockout strains, complex substrate uptake patterns, and dynamic metabolic flux distributions. Cybernetic theory builds...
Yeast batch fermentation is widely used in industrial biotechnology, yet its performance is strongly influenced by temperature and nitrogen availability, which affect growth kinetics and metabolite production. The development of predictive models tha...
Building a biologic model that describes the behavior of a cell in biologic systems is aimed at understanding the physiology of the cell, predicting the production of enzymes and metabolites, and providing a suitable data that is valid for bio-produc...
The green biosynthesis of adipic acid, an important monomer of nylon 6,6, has become a research hotspot. α-Aminoadipate is a key intermediate in the metabolic pathway that converts L-lysine to produce adipic acid. In addition, metabolic flux an...
A specific phenotypic variant of obesity is metabolically healthy (MHO), which is characterized by normal blood pressure and lipid and glucose profiles, in contrast to the metabolically unhealthy variant (MUO). The genetic causes underlying the diffe...
The optimization of metabolic reaction modifications for the production of target compounds is a complex computational problem whose execution time increases exponentially with the number of metabolic reactions. Therefore, practical technologies are...
In the wine industry, task planning is based on decision-making processes that are influenced by technical and organizational constraints as well as regulatory limitations. A characteristic constraint inherent to this sector concerns occupational ris...
Although cancer has historically been regarded as a cell proliferation disorder, it has recently been considered a metabolic disease. The first discovery of metabolic alterations in cancer cells refers to Otto Warburg’s observations. Cancer met...
Rice bran, a by-product of rice milling, is abundant in bioactive molecules and is highly recognized for its health-promoting properties, particularly in improving metabolic conditions. Building on this knowledge, we aimed to optimize the extraction...
Bioprocesses are increasingly used for the production of high added value products. Microorganisms are used in bioprocesses to mediate or catalyze the necessary reactions. This makes bioprocesses highly nonlinear and the governing mechanisms are comp...
In intermittent sports, tracking technologies are commonly used to monitor external and internal loads. The metabolic power model solely uses speed and acceleration data to simulate metabolic power, oxygen uptake, energy expenditure, and aerobic&ndas...
Biomass residue and waste stream bioconversion is a key pillar for successful transition toward sustainable bioeconomy. Spent microbial biomass (SMB) is a unique type of nutrient-rich residue generated from fermentation. This study addresses the wast...
Cycling stands as one of the most widely embraced leisure activities and serves purposes such as exercise, rehabilitation, and commuting. This study aimed to assess the feasibility of assisting three unimpaired participants (age: 34.0 ± 7.9 ye...
The coexistence of temperature changes and air pollution poses a severe global environmental issue, exacerbating health burdens. The aim of this study was to clarify the combined effects of ambient PM2.5 and cold exposure on the development of metabo...
The inherent complexity of modern supply chains obscures significant hidden CO2 and Water Pollution Equivalent (WPE) emissions, presenting mounting challenges for integrated environmental governance. While prior research has largely treated carbon an...
Saccharopolyspora erythraea is considered to be an effective host for erythromycin. However, little is known about the regulation in terms of its metabolism. To develop an accurate model-driven strategy for the efficient production of erythromycin, a...
Enzyme kinetic constants in vivo are largely unknown, which limits the construction of large metabolic models. Given measured metabolic fluxes, metabolite concentrations, and enzyme concentrations, these constants may be inferred by model fitting, bu...
L-tryptophan is an essential aromatic amino acid that has been widely used in medicine, food, and animal feed. Microbial biosynthesis of L-tryptophan through metabolic engineering approaches represents a sustainable, cost-effective, and environmental...
L-tryptophan is an essential amino acid widely used in the pharmaceutical and feed industries. Enhancing its production in microorganisms necessitates activating and inactivating specific genes to direct more resources toward its synthesis. In this s...
Pancreatic cancer is frequently accompanied by cancer-associated cachexia, a debilitating metabolic syndrome marked by progressive skeletal muscle wasting and systemic metabolic dysfunction. This study presents a systems biology framework to simultan...
Mandelic acid (MA) is a valuable α-hydroxy acid with applications in pharmaceuticals, cosmetics, and fine chemicals. While chemical synthesis is well established, concerns over toxicity and sustainability have driven interest in microbial produ...
Buckwheat possesses significant nutritional content and contains different bioactive compounds, such as total flavonoids, which enhance its appeal to consumers. This study employed single-factor experiments and the response surface methodology to ide...
Cordycepin, a bioactive adenosine analog, holds promise in pharmaceutical and health product development. However, large-scale production remains constrained by the limitations of natural producers, Cordyceps spp. Herein, we report the reconstruction...
Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are highly effective in the management of obesity; however, their efficacy and tolerability may be further optimized through complementary nutritional strategies. Such interventions may address key...
The metabolic engineering of pathways has been used extensively to produce molecules of interest on an industrial scale. Methods like gene regulation or substrate channeling helped to improve the desired product yield. Cell-free systems are used to o...
Cell-free systems offer many advantages for the study, manipulation and modeling of metabolism compared to in vivo processes. Many of the challenges confronting genome-scale kinetic modeling can potentially be overcome in a cell-free system. For exam...
The purpose of this study was to investigate the effects of the optimization of dietary energy supply on the growth performance, nutrient digestibility, energy metabolism, nutrient oxidation, slaughter performance, and meat quality of growing and fat...
Production of small molecule drugs in a recombinant host is becoming an increasingly popular alternative to chemical synthesis or production in natural hosts such as plants due to the ease of growing microorganisms with higher titers and less cost. W...
9 December 2022
Microalgae are microscopic photosynthetic organisms frequently found in fresh and marine water ecosystems. Various microalgal species have been considered a reservoir of diverse health-value products, including vitamins, proteins, lipids, and polysac...
Chimeric antigen receptor (CAR) T and CAR NK cell therapies opened new avenues for cancer treatment. Although original successes of CAR T and CAR NK cells for the treatment of hematological malignancies were extraordinary, several obstacles have sinc...
L-tryptophan (L-trp), produced through bio-manufacturing, is widely used in the pharmaceutical and food industries. Based on the previously developed L-trp-producing strain, this study significantly improved the titer and yield of L-trp, through meta...
Bio-cyanidation, as a sustainable and effective method to extract gold from primary and secondary resources, has attracted attention because of its environmental friendliness and economic benefits. The effect of amino acids on bio-cyanide production...
Phlorotannins are phloroglucinol-based phenolic compounds, occurring particularly in brown macroalgae, that have been recognized for their promising bioactive properties. In this study, the extraction of phlorotannins from Fucus vesiculosus was evalu...
There are numerous computational tools which support the design of new potential ligands. They assist in the evaluation of potential compound activity as well as in the optimization of compound physicochemical and pharmacokinetic properties. Nowadays...
Three-dimensional cancer models, such as spheroids, are increasingly being used to study cancer metabolism because they can better recapitulate the molecular and physiological aspects of the tumor architecture than conventional monolayer cultures. Al...
The increasing diffusion of herbal products is posing new questions: why are products so often different in their composition and efficacy? Which approach is more suitable to increase the biochemical productivity of medicinal plants with large-scale,...
Background/Objectives: The tacrolimus (Tac) concentration-to-dose ratio (C/D ratio) has been described as a predictive marker for several outcome parameters after renal transplantation (RTx). Different C/D ratio values are used to define fast (low C/...
of 104