Search Results (3,392)

The search for sustainable and ethical alternatives to conventional protein production has become increasingly important due to climate change, population growth, and the need to reduce the environmental impact of food systems. This work explores the development of animal-free proteins produced in laboratory settings using innovative technologies such as precision fermentation, submerged fermentation, plant cell culture, and molecular engineering. These methods enable the production of high-quality proteins without relying on animal farming or large-scale traditional agriculture. In addition to reviewing traditional plant-based protein sources and their nutritional limitations, the study highlights novel protein sources derived from fungi, algae, and bacteria, focusing on their nutritional profiles, production methods, and challenges related to digestibility, safety, and consumer perception. Special attention is given to downstream processing techniques that preserve protein functionality and enhance key food attributes such as texture, flavor, and stability. The use of agro-industrial residues is also discussed as a strategy to improve sustainability and economic viability. Key barriers to large-scale implementation, including production costs, regulatory approval, and consumer acceptance, are addressed, alongside emerging applications beyond food, such as cosmetics, animal nutrition, and biodegradable materials. Overall, animal-free proteins represent a promising path toward a more sustainable, resilient, and ethical global food system. Full article

The determination of pesticide residues at trace levels in complex environmental matrices requires robust and reliable analytical methodologies. In this study, a multi-residue method for determining 48 pesticides across different chemical classes in agricultural soils was developed and validated using a modified QuEChERS extraction followed by GC-MS/MS analysis. The method exhibited good linearity (R² ≥ 0.9871) over a concentration range of 5–600 µg/kg. Matrix effects were observed for several compounds, requiring matrix-matched calibration for accurate quantification. Recoveries fulfilled the recommended 70−120% criterion in 91.0% of the evaluated cases, while more than 98% of the relative standard deviation values were ≤20%, demonstrating satisfactory overall accuracy and precision according to SANTE/2020/12830 (Rev. 2) guidelines. The validated method was subsequently applied to tropical agricultural soils from Puerto Carreño (Colombian Orinoquia), which exhibited predominantly sandy textures (>80% sand), slightly acidic pH (5.2−5.7), low total organic carbon (0.1−0.8%), and low cation exchange capacity (5.6−8.1 meq/100 g). No pesticide residues were detected in the analyzed samples. The proposed method provides a reliable analytical framework for multi-class pesticide determination in tropical agricultural soils and contributes to the application of QuEChERS-based methodologies in underrepresented tropical soil matrices. Full article

This PRISMA-guided scoping review examines the use of waste materials in civil engineering as a sustainable approach to reducing environmental impacts, conserving natural resources, and supporting circular economy principles. The rapid growth of urbanization, industrialization, mining, and agricultural activities generates large amounts of waste materials, including fly ash, ground granulated blast-furnace slag, bauxite residue, mining tailings, waste rock, acid-mine drainage sludge, waste plastics, post-consumer vulcanized rubber, recycled construction materials, and agricultural ashes. The disposal of these materials often creates serious environmental and land-use problems, making their reuse increasingly important. In this context, civil engineering is one of the most promising sectors for large-scale waste valorization because of its high material demand and its ability to use different waste streams into practical applications such as concrete and cementitious systems, pavement and asphalt engineering, geotechnical works, and other infrastructure sectors. This review critically evaluates the global availability, material characteristics, engineering applications, environmental and economic benefits, recent advances, and key challenges related to major industrial, mining, agricultural, polymeric, and construction-derived wastes. Although significant progress has been made in this field, wider implementation is still limited by variations in material properties, technical and environmental challenges, economic constraints, and limited field validation of long-term performance. By bringing together current knowledge from different waste streams and civil engineering sectors, this review highlights important research gaps and future directions to support more sustainable, resilient, and resource-efficient infrastructure development. The effective use of waste materials in civil engineering can play an important role in reducing carbon emissions, improving resource efficiency, and supporting global sustainability. Full article

Hexavalent chromium (Cr(VI)) is a highly toxic, non-biodegradable, and carcinogenic heavy metal. Its continuous release into aquatic ecosystems demands efficient, low-cost adsorbents. In this study, orange peel and peanut shell residues were thermally modified at 250 °C to enhance Cr(VI) remediation. Structural characterization confirmed that low-temperature calcination transforms raw agroindustrial wastes into functional biochars with a chemical architecture primed for cooperative Cr(VI) removal. N₂ physisorption revealed a hierarchical mesoporous network with average pore diameters of 30.6 nm (calcined orange peel) and 15.4 nm (calcined peanut shell), despite low specific surface areas. Batch adsorption experiments demonstrated that removal kinetics reached equilibrium within 5 min for the modified biochars. Isotherm modeling showed that the adsorption process was best described by the Freundlich and Sips models. The calculated Sips heterogeneity factors (β_S > 1) provided evidence of a cooperative multi-layer adsorption mechanism, attributed to the induced mesoporosity: initial chemisorption at high-energy sites facilitates the continuous anchoring of additional Cr(VI) ions without premature saturation. Ultimately, this study demonstrates that low-temperature calcination is a viable strategy to transform agricultural waste into kinetically efficient, cooperative adsorbents for wastewater treatment. Full article

Fillers (<0.075 mm) in hot mix asphalt (HMA) play a pivotal role in optimizing bitumen content, filling voids, and improving mechanical performance. In many agricultural countries, large quantities of rice and wheat husk waste are produced, while the road construction industry faces material shortages of conventional filler materials and related performance challenges. This study evaluates the feasibility of using rice husk (RH) and wheat husk (WH) fillers on HMA performance. Unlike previous studies that primarily focused on ash-derived agricultural residues, this work investigates the direct utilization of raw husk materials, eliminating the need for energy-intensive processing. Few studies directly examine the aggregate gradation and binder concentration with respect to rice and wheat husk ash. As a result, the relative effectiveness of these two agricultural waste fillers in improving the volumetric and Marshall properties of asphalt mixtures is yet unknown. Fifteen mixtures with varying bitumen contents (3.0–5.0%) were tested to determine the optimum bitumen content (OBC). Subsequently, modified mixtures were prepared at the OBC using RH and WH fillers at five replacement levels (5.0–15.0%). The Marshall Mix design method was employed to assess stability, flow, density, and air voids content. The control mixture showed a Marshall stability of 14.86 kN, flow of 3.52 mm, density of 2.342 g/cm³, and air voids of 2.9%. At their optimum filler contents (i.e., 10.33% for RH and 10.43% for WH), the modified mixtures achieved higher Marshall stability (14.96 kN and 15.06 kN, respectively), with flow values of 3.51 mm and 2.83 mm, and densities of 2.335 g/cm³ and 2.330 g/cm³. Statistical analysis using ANOVA at the OBC confirmed that RH and WH fillers can be used as alternative fillers in HMA without adversely affecting Marshall performance, while contributing to agricultural waste valorization and resource conservation. Full article

The high-value utilization market for crop straw renders the development of stubble management technology crucial. This study aims to reduce the energy consumption of L-shaped rotary blades during stubble-breaking. Based on a theory analysis of the rotary-cutting operation process, this study involved the burial of the in situ maize root–soil composite in an indoor soil bin, and investigated the effects of rotary speed (275, 330, 385, 440 rpm) and working depth (50, 85, 120 mm) on torque, power, and energy. Field verification yields an overall average relative error of 2.76% across six replicates, verifying that the indoor test method can reliably reproduce field cutting conditions. As the high-speed video images show, a reduction in rotary speed coupled with an augmentation in working depth has the potential to result in residue entanglement and secondary cutting, thereby leading to an escalation in consumption. As the working depth increased, peak torque appeared at a deeper penetration position. The analysis of the computer-aided geometric model section of the root–soil composite indicated that the diameter of the branching root was the primary factor influencing peak torque. At a working depth of 85 mm, the average power savings ranged from 2.26% to 24.8% compared to 50 mm and 120 mm. Despite the increase in average power, peak power, and specific energy requirements at all operational depths with increasing rotary speed, torque reached its minimum at 385 rpm. At 385 rpm, average torque hits its minimum to mitigate component wear, though power and specific energy rise monotonically with rotational speed. The multi-index evaluation balancing mechanical load, energy loss, and residue delivery identifies 385 rpm paired with 85 mm depth as the optimal parameter set. The optimized parameter combination delivers a quantifiable sustainable residue management scheme that balances ecological residue treatment and economic machinery operation costs, supporting low-carbon, sustainable production. Full article

The substantial volume of agrifood processing side streams represents a global sustainability challenge that requires transformation of agricultural residues into high-value molecular components. Grape pomace (GP) is a major winemaking co-product whose chemical diversity remains underutilized due to a lack of high-resolution structural data. This study applies advanced analytical platforms to provide a comprehensive molecular characterization of oligosaccharides (OS) and phenolics in GP from four grape varieties (Chardonnay, Sauvignon Blanc, Pinot Noir, and Merlot) from California. Results demonstrate that the molecular signature of the material is highly dependent on the variety and its corresponding processing; white wine pomaces exhibited significantly higher residual sugars and generally greater OS diversity compared to red wine pomaces. Using LC-Q-ToF-MS, 39 oligosaccharides were identified, primarily composed of hexoses and pentoses. Characterization of the OS building blocks via LC-QqQ-MS revealed the dominance of glucose and fructose, followed by arabinose and xylose. In parallel, targeted phenolics quantification by UPLC-DAD showed that (+)-catechin and (−)-epicatechin accounted for up to 50% of quantified phenolics in Chardonnay pomace and 45% in Pinot Noir pomace. This work highlights the molecular intricacies of GP, providing a compositional foundation for its targeted valorization. Full article

Anaerobic digestion (AD) is an effective technology for sustainable waste management, renewable energy production and resource recovery within a circular economy. This study offers a systematic bibliometric review of global advances in AD research and assesses their relevance to Ghana. Using the PRISMA framework, the literature from 2011 to 2025 was sourced from the Scopus database and analysed through bibliometric and thematic methods. The review emphasises four key factors affecting AD performance: municipal solid waste as feedstock, pretreatment technologies, biochemical methane potential (BMP) assessment and process optimisation. Studies were included if they addressed any of these themes. Non-English publications, inaccessible full texts and papers lacking bibliographic metadata were excluded. After screening 3424 records, 61 studies were included in the systematic review. After metadata screening, 3374 of the 3424 retrieved records were retained for bibliometric analysis. Results show that municipal solid waste, food waste, agricultural residues and sewage sludge are promising sources for biogas generation. Pretreatment techniques, including thermal, chemical, mechanical and biological, significantly enhance substrate biodegradability and methane production. BMP assessment is a reliable way to gauge feedstock suitability and energy recovery potential. Optimising parameters like pH, temperature, organic loading, hydraulic retention time and co-digestion ratios improves process stability and biogas yield. The review highlights the increasing use of modelling and optimisation to boost digester performance and facilitate scale-up. In Ghana, abundant organic waste offers significant opportunities for biogas development. Employing advanced feedstock characterisation, pretreatment, BMP evaluation and optimisation can improve AD efficiency, support renewable energy, reduce waste disposal issues and promote Ghana’s shift toward a sustainable circular bioeconomy. Full article

Wear prediction for agricultural soil-engaging components is computationally demanding when discrete element method (DEM) simulations are repeatedly used for design evaluation and operating-parameter screening. In this study, an Archard-inspired Gaussian process regression (GPR) residual-learning surrogate was developed for rapid prediction of the total wear volume calculated by EDEM for a ploughshare. The physical prior was a monotonic operational-parameter proxy motivated by the load and sliding trends in Archard theory, which did not directly use DEM-derived normal force, sliding distance, or frictional work. A soil–ploughshare interaction model was used to generate 100 full-factorial samples with tillage depth, tillage speed, and penetration angle as inputs. The Archard-inspired prior, cubic polynomial Ridge regression, standard GPR, and prior-guided residual GPR were evaluated by cross-validation, repeated random splits, and boundary-level extrapolation tests. Across 30 repeated 90%/10% splits, standard and Archard-inspired GPR achieved mean R² values of 0.9927 ± 0.0014 and 0.9908 ± 0.0021, respectively. In the 200 mm tillage-depth extrapolation test, the latter performed best, with R² = 0.9752, RMSE = 0.000252 mm³, and MAPE = 2.68%; however, the former was more accurate in the tillage-speed and penetration-angle extrapolation tests, and the 48% interval coverage of the prior-guided model in the penetration-angle test indicated overconfidence when the prior was biassed. These results show a conditional, rather than universal, benefit of the Archard-inspired prior: it improved extrapolation plausibility for the load-dominated tillage-depth case but did not improve all boundary predictions. The surrogate predicts EDEM-simulated wear, and its engineering validity depends on DEM calibration, the selected wear coefficient, and future soil-bin or field validation. Full article

The accumulation of shipwrecked wood in coastal ecosystems represents an important environmental challenge due to its effects on coastal dynamics, ecosystem functioning, and waste management. The valorization of this lignocellulosic residue through thermochemical conversion offers a sustainable alternative for transforming an underutilized biomass into value-added products. This study evaluated the potential of biochar produced from shipwrecked wood collected in the Punta Roca sector, municipality of Puerto Colombia (Atlántico), as an organic soil amendment. The methodology included the physicochemical characterization of the biochar, as well as analysis of its specific surface area (BET), morphology (SEM), and elemental composition (EDAX). Among the most relevant physicochemical results, the biochar exhibited an organic matter content of 87.85%, an alkaline pH of 10.29, and low moisture content (9.21%), suggesting stability and the capacity to modify soil acidity. Regarding specific surface area, the BET area increased significantly from 0.77 m²/g in untreated wood to 61.61 m²/g in the biochar, indicating a notable enhancement in adsorption capacity. Porosity analysis revealed a decrease in pore size, which may favor the retention of nutrients and water. Elemental composition analysis revealed a high carbon content (82.65%) and oxygen content (15.32%), accompanied by trace amounts of elements such as Na, Mg, Si, Cl, K, and Ca. These results confirm that biochar derived from shipwrecked wood possesses physicochemical characteristics suitable for application as an organic amendment in agricultural soils. Overall, these findings demonstrate that biochar derived from shipwrecked wood is a promising alternative not only as an organic soil amendment but also as a strategy for coastal waste valorization, the promotion of circular economy principles, and the development of more sustainable agricultural systems. Full article

Hippodamia variegata is a key natural enemy of various aphid pests in agricultural ecosystems, and its foraging and oviposition behaviors are strongly dependent on chemical signals from prey aphids and host plants. In this study, we functionally characterized the chemosensory protein HvarCSP8 to elucidate its potential role in mediating olfactory responses to aphid-induced plant volatiles. The expression profiles of HvarCSP8 were examined by quantitative real-time PCR (qRT-PCR) across developmental stages and sexes; the recombinant HvarCSP8 was purified; the potential ligands specificity and affinity were studied using fluorescence-based competitive binding assays; and structural characteristics were obtained via homology modeling and molecular docking simulations. The results demonstrated that HvarCSP8 exhibits pronounced sex- and tissue-specific expression, with significant upregulation in adult antennae, particularly in females. The purified protein exhibited selective binding affinity to multiple aphid-induced plant volatiles, such as α-Farnesene, α-Terpinene, and 2-Ethyl-1-hexanol. Molecular docking revealed that HvarCSP8 interacts with these ligands through multiple amino acid binding sites, among which hydrophobic residues such as VAL29, LEU61, ILE64, and LEU65 frequently occur in binding sites for multiple ligands, suggesting that these residues play important roles in binding. These findings indicate that HvarCSP8 may be involved in the olfactory detection of aphid-induced plant volatiles in H. variegata, providing a candidate target for the development of behavior-based biological control strategies against aphid pests. Full article

The microbial fermentation of mulberry branch residues offers a potential strategy for sustainable lignocellulosic biomass valorization. This study integrated 16S rRNA gene sequencing and untargeted LC–MS metabolomics to characterize temporal microbial and metabolic changes during a 12-day solid-state fermentation at 35 °C using a defined consortium of lactic acid bacteria, Bacillus subtilis, and Saccharomyces cerevisiae. Microbial community analysis revealed distinct temporal succession, with Bacillus accounting for 19.5% of the bacterial community during early fermentation, followed by increasingly diverse assemblages. Exploratory machine learning analysis ranked Azotobacter and Kyrpidia among the genera contributing most strongly to temporal differentiation. FAPROTAX-based predictions indicated that chemoheterotrophy-related functions remained prevalent across fermentation stages, although these predictions do not represent direct functional activity. Untargeted metabolomics detected 2782 putatively annotated features, dominated by lipids (15.1%), organic acids (13.8%), and phenylpropanoids (13.2%). Correlation analysis identified temporal associations between bacterial genera and metabolite classes, including associations of Geobacillus with alkaloids and glycerophospholipids. Exploratory OPLS-DA further highlighted pseudouridine and 3-methylxanthine as discriminatory features across fermentation stages. These findings provide a descriptive multi-omics overview of microbial succession and metabolic variation during mulberry branch residue fermentation. Full article

Plant cell, tissue, and organ cultures are increasingly used as controlled platforms for producing pharmaceutically important specialized metabolites. Their productivity can be improved through elicitors, plant biostimulant-like substances, microbial inputs, and nano-enabled delivery systems. However, the regulatory interpretation of these inputs is not determined only by their biological mechanism. It also depends on product composition, formulation, intended use, exposure scenario, and label claim. This review analyzes how elicitors, plant biostimulants, biofertilizer- and bioinput-related concepts, and nano-enabled inputs are positioned within selected regulatory frameworks in the European Union, United States, Canada, Brazil, India, China, and Mexico. The analysis shows that current frameworks increasingly emphasize product identity, efficacy substantiation, safety evidence, and labeling, but they remain fragmented for multifunctional materials that may simultaneously affect nutrient-related processes, abiotic stress tolerance, defense signaling, growth regulation, and specialized metabolism. Nano-enabled and encapsulated formulations create additional challenges because they can modify uptake, persistence, bioavailability, release behavior, residue profiles, and non-target exposure compared with conventional formulations. A clearer distinction is therefore needed between contained biotechnological use in plant biofactories and open-environment agricultural applications. Future regulatory development should move toward formulation-level assessment, claim-specific evidence, nanospecific characterization, traceability, and harmonized terminology to support innovation while ensuring safety, reproducibility, and regulatory clarity. Full article

Shifting climatic conditions challenge simplified, yield-oriented agriculture, particularly in fragile mountain regions. In the Ecuadorian Andes, agriculture functions as a socio-ecological system shaped by biocultural relationships integrating production, agrobiodiversity, and farmer decision-making. This study examines how climate variability interacts with crop phenology and management practices within ancestral associated cropping systems (chakras). The analysis focuses on maize, common bean, faba bean, and potato during the 2024–2025 agricultural cycle in Imantag, Ecuador. A total of 30 native, introduced, and improved varieties were sown in traditional rows (wachos) and monitored within a single 420.8 m² Andean chakra. Using multiple ordinary least squares (OLS) regression (n = 30; R² = 0.440, adj. R² = 0.351, F (4,25) = 4.914, p = 0.005), results show that maize significantly outperformed the faba bean reference group (β = 20.34, p = 0.017), while common bean showed intermediate performance (β = 15.31, p = 0.048). Seed mass at planting was positively associated with relative yield (β = 6.71, p = 0.069), highlighting early-stage management decisions. Elevated maximum temperatures during maturation negatively affected yield (r = −0.42, p = 0.020), while accumulated precipitation had a positive effect (r = +0.45, p = 0.014). Model quality criteria, including VIF diagnostics (max. VIF = 3.37), Shapiro–Wilk residual normality test (W = 0.983, p = 0.896), and cross-validation using Ridge and Lasso regression, confirm the robustness of the statistical findings. These findings demonstrate that chakras function as adaptive socioecological systems that enhance productivity, agrobiodiversity conservation, and resilience under climate variability. Full article