Search Results (154)

Tuta absoluta (Meyrick) is a globally invasive lepidopteran pest that has developed resistance to multiple classes of chemical insecticides, posing major challenges for the sustainable production of Solanaceae crops. In this study, we investigated the physiological and molecular responses of T. absoluta larvae to infection by the entomopathogenic bacterium Serratia marcescens (Bizio) strain Tapa21, which was isolated from naturally infected larvae and characterized through phenotypic, molecular, and phylogenetic analyses. Laboratory bioassays demonstrated dose- and time-dependent mortality of T. absoluta larvae, with mortality reaching nearly 80% at the highest Tapa21 concentration at 120 h post-infection (hpi), with a median lethal concentration (LC₅₀) of Optical Density (OD)₆₀₀ = 0.52 and a median lethal time (LT₅₀) of 5.2 d. RNA-Seq was performed, revealing 493 differentially expressed genes (DEGs), including 304 up-regulated and 189 down-regulated transcripts. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated activation of detoxification-related enzymes, lysosome- and immune-associated pathways, and metabolic reprogramming, suggesting coordinated defense responses. A subset of genes, randomly selected across expression levels, was validated by RT-qPCR, corroborating the transcriptomic results. These results delineate the molecular mechanisms by which T. absoluta reshapes its physiological state during bacterial challenge and provide insight into how entomopathogenic strain Tapa21 disrupts host homeostasis. Such a mechanistic understanding could potentially contribute to sustainable and integrated pest management (IPM) strategies. Full article

High-grade serous ovarian carcinoma is the most common and aggressive form of ovarian cancer, accounting for over 60% of cases and nearly 75% of deaths, mainly due to late diagnosis and tumor aggressiveness. Standard treatment is platinum-based chemotherapy with paclitaxel, but relapse is frequent. This study aimed to identify prognostic biomarkers for patients with poor survival outcomes after Taxol treatment using bioinformatics analysis. We examined the effects of TGFB2 mRNA expression and other markers on overall survival in serous ovarian cancer using the TCGA database, applying a multivariate Cox model that included interaction terms to identify TGFB2-dependent and independent prognostic markers, and controlling for age and treatment type. Candidate TGFB2-independent prognostic markers from TCGA were further validated using patient data from the KMplotter database. High TGFB2 mRNA expression emerged as a prognostic biomarker for three potential gene targets (TRPV4, STAU2, and HOXC4) associated with improved OS at low levels of gene target expression, we identified four additional markers (CLIC3, ANPEP/LAP1, RIN2, and EMP1) that exhibited a TGFB2-independent negative correlation between mRNA expression and OS across the full spectrum of gene expression values in the ovarian cancer cohort validated using independent dataset from KMplotter, for Taxol-treated ovarian cancer patients. This study proposes a panel of potential prognostic biomarkers for the treatment of ovarian cancer patients, particularly by leveraging TGFB2-dependent mRNA expression as a significant biomarker, alongside four additional TGFB2-independent prognostic markers, for patients undergoing Taxol-based therapies. Future prospective clinical trials will be required to validate these prognostic markers. Full article

Fluorescent nanoprobes operating in the NIR-II window have gained considerable attention for biomedical imaging because of their deep-tissue penetration, reduced scattering, and high spatial resolution. Their tunable optical behavior, flexible surface chemistry, and capacity for multifunctional design enable sensitive detection and targeted visualization of biological structures in vivo. This review highlights recent advances in the design and optical engineering of four widely studied NIR-II nanoprobe families: quantum dots, carbon dots, upconversion nanoparticles, and dye-doped silica nanoparticles. These materials were selected because they offer well-defined architectures, controllable emission properties, and substantial mechanistic insight supporting discussions of imaging performance and translational potential. Particular focus is placed on emerging strategies for activatable, targeted, and ratiometric probe construction. Recent efforts addressing biosafety, large-scale synthesis, optical stability, and early preclinical validation are also summarized to clarify the current progress and remaining challenges that influence clinical readiness. By outlining these developments, this review provides an updated and focused perspective on how engineered NIR-II nanoprobes are advancing toward practical use in biomedical imaging and precision diagnostics. Full article

Maraging steels encounter tremendous aerospace applications, such as in landing gears, rocket motor casing, pressure vessels, satellite launch vehicles, etc. Laser welding is considered one of the most effective manufacturing processes due to its minimal instances of wider heat-affected zones (HAZs), precipitate accumulation, and other benefits. However, it should also be noted that their severe effect is still evident in terms of the tensile strength and fatigue strength of laser-welded maraging steel. This paper provides a critical review of the evolution of microstructural features and mechanical properties of laser-welded maraging steel, including corresponding factors in terms of microstructures and the formation of reverted austenite, as well as precipitation hardening from various studies on maraging steels. We examined the influence of precipitation, reverted austenite, welding, and post-weld heat treatment on mechanical properties like hardness, tensile strength, yield strength, elongation, and fatigue strength of laser-welded maraging steel. It is worth mentioning that the laser welding process is generally insufficient for welding sheets with a thickness over 10 mm or those requiring multi-pass welding. The reheating process becomes unfavorable for maraging steel in the multi-pass welding process since it may induce localized heat treatment. Although hybrid welding may resolve an arising thickness issue, the reversion of austenite and complexity are still difficult to overcome due to the dual nature of welding processes, resulting from the use of both arc and laser. Furthermore, maraging steel produced via additive manufacturing tends to avoid austenite reversion with effective heat treatment prior to any welding process. Post-weld heat treatment and cryogenic treatment have been found to be favorable for desired reverted austenite formation. Finally, the proposed constructive framework specifically applies to the welding process of maraging steel, particularly for aerospace applications. Full article

Prostate Cancer (PCa) screening using Prostate-Specific Antigen (PSA) has significantly improved early detection but has also led to substantial overdiagnosis and overtreatment, particularly of indolent tumors. While active surveillance and focal therapies have mitigated some harms, distinguishing aggressive from non-threatening disease remains a critical clinical challenge. Emerging evidence highlights the epigenetic regulation of the human Telomerase Reverse Transcriptase (hTERT) gene as a promising biomarker for risk stratification. Cancer-specific hypermethylation within the TERT Hypermethylated Oncological Region (THOR) and the broader CpG island termed “Acheron” correlates with hTERT reactivation, tumor progression, and adverse outcomes. Additionally, suppression of the long non-coding (lnc) RNA human TERT Antisense Promoter-Associated (hTAPAS) contributes to the derepression of hTERT, providing a mechanistic link between DNA methylation and telomerase activation. Collectively, these epigenetic signatures, referred to as EpihTERT, can be detected in tissue and liquid biopsies, offering non-invasive assessment of tumor aggressiveness. Integration of EpihTERT profiling into clinical practice may enhance early diagnosis, refine patient selection for intervention, and reduce unnecessary treatments, bridging the gap between overdiagnosis and timely identification of clinically significant disease. Prospective multicenter validation is warranted to establish EpihTERT as a robust, translational biomarker in PCa management. Full article

The integration of smart nanomaterials into pharmaceutics has transformed approaches to disease diagnosis, targeted therapy, and tissue regeneration. These nanoscale materials exhibit unique features such as controlled responsiveness, biocompatibility, and precise site-specific action, offering new possibilities for personalized healthcare. This review provides a comprehensive overview of recent advances in the design and application of functional nanomaterials, including nanoparticle-based drug carriers, responsive hydrogels, and nanostructured scaffolds. Special focus is placed on stimuli-triggered systems that achieve controlled drug release and localized therapeutic effects. In addition, the review explores how these materials enhance diagnostic imaging and support tissue regeneration through adaptive and multifunctional designs. Importantly, this work uniquely integrates stimuli-responsive nanomaterials across therapeutic, imaging, and regenerative domains, providing a unified view of their biomedical potential. The challenges of clinical translation, large-scale synthesis, and regulatory approval are critically analyzed to outline future directions for research and real-world implementation. Overall, this review highlights the pivotal role of smart nanomaterials in advancing modern pharmaceutics toward more effective and patient-centered therapies. Full article

Nanoparticles (NPs) have significantly changed the field of drug delivery, offering control over pharmacokinetics, biodistribution, and targeted therapy. Among these, ultrasmall nanoparticles (USNPs) with sizes of approximately 5–15 nm have garnered significant interest due to their unique physicochemical properties, including enhanced cellular uptake, deeper tissue penetration, and prolonged systemic circulation. This review explores the fundamental principles governing sub-15 nm nanoparticles, their classification, and their distinctive advantages in pharmaceutical applications. Various types of nanoparticles, including polymeric, lipid-based, metallic, and carbon-based nanosystems, are examined in the context of drug delivery in cancer therapy. We detail how sub-15 nm polymeric nanoparticles (PNPs) are emerging as transformative drug delivery platforms for cancer therapy. The impact of nanoparticle size, surface modifications, and biocompatibility on therapeutic performance is critically analyzed. Furthermore, we discuss emerging applications of these ultrasmall nanoparticles in cancer therapy, neurological disorders, vaccine delivery, and imaging. Despite their promise, key challenges such as stability, aggregation, toxicity, and regulatory concerns remain significant hurdles for clinical translation. This review provides insights into the potential of 5–15 nm nanoparticles to reshape modern drug delivery and highlights future directions for research and development in this rapidly evolving field. Full article

Human rights frameworks have historically emphasised physical integrity, yet psychological integrity, the right to mental stability, identity, and emotional safety all remain neglected in health policy and law. This narrative review and commentary argues that psychological integrity is inseparable from ecological integrity, and that contemporary mental health crises are rooted in ruptured human–nature attachments. Drawing on Mother Nature Attachment Theory (MNAT) and supported by emerging empirical evidence, this review traces a trajectory from pre-attachment, through rupture via colonisation, displacement, and ecological collapse, to reconnection through cultural and ecological repair. Gaza exemplifies a contemporary site of deliberate ecological–psychological rupture, where environmental destruction compounds trauma and erodes cultural continuity. In contrast, Indigenous frameworks in Australasia, such as Te Whare Tapa Whā, provide culturally grounded models of reconnection that demonstrate how ecological repair and psychological restoration can proceed together. These contrasting cases illustrate MNAT’s trajectory and emphasise that safeguarding psychological integrity requires embedding ecological security into public health systems. The review concludes that planetary mental health depends on recognising healing of mind and Earth as an indivisible task. Healing mind and Earth must be understood as a single, urgent task within planetary public mental health. Full article

We developed a forecast model for cyanobacteria bloom formation in two contrasting inland lakes in Australia by combining in situ sampling and continuous remote sensing hyperspectral reflectance (HydraSpectra) with hydrodynamic and algal growth models. Cyanobacterial distribution of a buoyant species was simulated with an algal growth model, driven by forecasted meteorological data, and modeled temperature stratification and mixing dynamics from a one-dimensional, vertical k-epsilon turbulence hydrodynamic model. The cyanobacteria model was re-initialized daily with measured cell counts derived from the hyperspectral reflectance data. Simulations of cyanobacterial concentrations (cell counts) reflected the dynamic mixing behavior in the lakes with daily phases of near-surface accumulation and subsequent daily mixing due to wind or night-time cooling. To determine the surface concentration of cyanobacteria on sub-daily time scales, it was demonstrated that the combined use of high-resolution water temperature profiles, HydraSpectra reflectance data, and a hydrodynamic model to quantify the mixing dynamics is essential. Overall, the model results demonstrated a prototype for a cyanobacteria short-term forecast model. Having these tools in place allows us to quantify the risks of cyanobacterial blooms in advance to inform options for lake management. Full article

Purpose: The synthesis of nanoscale particles with antibacterial properties has garnered significant attention in pharmaceutical research, driven by the escalating threat of antibiotic-resistant bacteria. This study investigates the antibacterial efficacy of Zn–Co ferrite nanoparticles against virulent, antibiotic-resistant, and biofilm-forming strains of Escherichia coli. Methods: Three nanoparticle variants—S1 (Zn_0.7Co_0.3Fe₂O₄), S2 (Zn_0.5Co_0.5Fe₂O₄), and S3 (Zn_0.3Co_0.7Fe₂O₄)—were synthesized using the solution combustion method by systematically varying the Zn:Co molar ratio. The Scanning Electron Micrograph, X-ray diffraction analysis, Complementary Fourier-transform infrared, Minimum Inhibitory Concentration, and Minimum Bactericidal Concentration were performed. Results: The SEM spectroscopy study revealed distinct morphological differences as a function of the cobalt substitution level within the spinel ferrite matrix. At the highest level of cobalt substitution (Zn_0.3Co_0.7Fe₂O₄), the microstructure displayed significant irregularities, with enhanced agglomeration and a notably broader particle size distribution. X-ray diffraction analysis confirmed the formation of crystalline structures, with an average crystallite size of 12.65 nm. Complementary Fourier-transform infrared spectroscopy revealed characteristic absorption bands in the 400–600 cm⁻¹ range, indicative of the cubic spinel structure of the ferrite nanoparticles. The higher-frequency band was associated with metal–oxide stretching in the tetrahedral sites, while the lower-frequency band corresponded to stretching in the octahedral sites. The Minimum Inhibitory Concentration and Minimum Bactericidal Concentration assays revealed that Zn–Co ferrite nanoparticles possess potent antibacterial activity against virulent, antibiotic-resistant, and biofilm-forming strains of E. coli. Conclusion: Increasing the molar ratio of Zn to Co enhances the antibacterial activity of the nanoparticles. These findings suggest that Zn–Co ferrite nanoparticles could serve as a promising alternative to conventional antibacterial agents for combating multidrug-resistant pathogenic bacteria in the future. Full article

Phytochemicals from medicinal plants offer significant therapeutic benefits, yet their clinical utility is often limited by poor solubility, instability, and low bioavailability. Nanotechnology presents a transformative approach to overcome these challenges by encapsulating phytochemicals in nanocarriers that enhance stability, targeted delivery, and controlled release. This review highlights major classes of phytochemicals such as polyphenols, flavonoids, and alkaloids and explores various nanocarrier systems including liposomes, polymeric nanoparticles, and hybrid platforms. It also discusses their mechanisms of action, improved pharmacokinetics, and disease-specific targeting. Further, the review examines clinical advancements, regulatory considerations, and emerging innovations such as smart nanocarriers, AI-driven formulation, and sustainable manufacturing. Nano-phytomedicine offers a promising path toward safer, more effective, and personalized therapies, bridging traditional herbal knowledge with modern biomedical technology. Full article

Carbon nanotubes (CNTs) have emerged as pivotal nanomaterials in sensing technologies owing to their unique structural, electrical, and mechanical properties. Their high aspect ratio, exceptional surface area, excellent electrical conductivity, and chemical tunability enable superior sensitivity and rapid response in various sensor platforms. This review presents a comprehensive overview of recent advancements in CNT-based sensors, encompassing both single-walled (SWCNTs) and multi-walled carbon nanotubes (MWCNTs). We discuss their functional roles in diverse sensing applications, including gas sensing, chemical detection, biosensing, and pressure/strain monitoring. Particular emphasis is placed on the mechanisms of sensing, such as changes in electrical conductivity, surface adsorption phenomena, molecular recognition, and piezoresistive effects. Furthermore, we explore strategies for enhancing sensitivity and selectivity through surface functionalization, hybrid material integration, and nanostructuring. The manuscript also covers the challenges of reproducibility, selectivity, and scalability that hinder commercial deployment. In addition, emerging directions such as flexible and wearable CNT-based sensors, and their role in real-time environmental, biomedical, and structural health monitoring systems, are critically analyzed. By outlining both current progress and existing limitations, this review underscores the transformative potential of CNTs in the design of next-generation sensing technologies across interdisciplinary domains. Full article

Barium stannate (BaSnO₃) has emerged as a promising alternative electron transport material owing to its superior electron mobility, resistance to UV degradation, and energy bandgap tunability, yet BaSnO₃-based perovskite solar cells have not reached the efficiency levels of TiO₂-based designs. This theoretical study presents a design-driven evaluation of BaSnO₃-based perovskite solar cell architectures, incorporating MAPbI₃ or FAMAPbI₃ perovskite materials, Spiro-OMeTAD, or Cu₂O hole transport materials as well as hole-free configurations, under varying light intensity. Using a systematic device modelling approach, we explore the influence of key design variables—such as layer thickness, donor density, and interface defect concentration—of BaSnO₃ and operating temperature on the power conversion efficiency (PCE). Among the proposed designs, the FTO/BaSnO₃/FAMAPbI₃/Cu₂O/Au heterostructure exhibits an exceptionally effective arrangement with PCE of 38.2% under concentrated light (10,000 W/m², or 10 Sun). The structure also demonstrates strong thermal robustness up to 400 K, with a low temperature coefficient of −0.078% K⁻¹. These results underscore the importance of material and structural optimisation in PSC design and highlight the role of high-mobility, thermally stable inorganic transport layers—BaSnO₃ as the electron transport material (ETM) and Cu₂O as the hole transport material (HTM)—in enabling efficient and stable photovoltaic performance under high irradiance. The study contributes valuable insights into the rational design of high-performance PSCs for emerging solar technologies. Full article