The gene flow rate in rice (Oryza sativa L.) is a critical factor for establishing safe isolation distances between genetically modified (GM) and non-GM varieties and for ensuring varietal purity in rice breeding programs. This study refines existing...
Despite the continuous and intensive development of laboratory techniques, a light microscope is still the most common tool used in pollen grains differentiation. However, microscopy is time-consuming and needs well-educated and experienced researche...
The genus Lolium has shown a high tendency to evolve resistance to herbicides. Considering the high level of allogamy of L. perenne and the importance of the wind as an agent for pollen dispersion, the aim of this work was to assess and quantify the...
Over the last decade, efforts to breed new Cannabis sativa L. cultivars with high Cannabidiol (CBD) and other non-psychoactive cannabinoids with low tetrahydrocannabinol (THC) levels have increased. In this context, the identification of the viabilit...
A transgenic winter triticale line expressing the uidA gene, encoding β-glucuronidase, was used to assess the pollen flow in field experiments over two consecutive vegetation seasons in central Poland. The experimental design included two variants of...
Regulatory compliance of experimental releases into the environment of not yet approved genetically modified plants often requires implementation of measures to reduce the dispersal of reproductive material. To study the impact of nets on pollen flow...
Norway spruce differs little in neutral genetic markers among populations and provenances often reported, but in terms of putative adaptive traits and their candidate genes, some clear differences have been observed. This has previously been shown fo...
Exploring the gene flow and its causes in complex habitats of forest trees is valuable for understanding species’ adaptive evolution. Pinus yunnanensis var. tenuifolia (PYT) is mainly distributed in the dry–hot valleys along the Nanpan-Ho...
The Laperrine’s olive is endemic to the Saharan Mountains. Adapted to arid environments, it may constitute a valuable genetic resource to improve water-stress tolerance in the cultivated olive. However, limited natural regeneration coupled with human...
Acer griseum (Franch.) Pax is an endangered species endemic to China, mainly scattered in the Qinling–Daba Mountains. The genetic diversity of 17 natural populations were analyzed by nuclear DNA (nDNA) and chloroplast DNA (cpDNA) to explore the...
The utilization of genotyping has gained significant popularity in tree improvement programs, aiding in enhancing the precision of breeding values, removing pedigree errors, the assessment of genetic diversity, and evaluating pollen contamination. Ou...
As for most other temperate broadleaved tree species, large-scale genetic inventories of pedunculate oak (Quercus robur L.) have focused on the plastidial genome, which showed the impact of post-glacial recolonization and manmade seed transfer. Howev...
In this study, 53 pear genotypes were evaluated, including 19 Asian varieties, 7 botanical species and 13 new interspecific hybrids. The ploidy level and nuclear DNA content were determined by flow cytometry. In addition, the morphological traits and...
Neolamarckia cadamba (Roxb.) Bosser is a fast-growing deciduous tree species and belongs to the Neolamarckia genus of the Rubiaceae family. This species has great economic and medical values in addition to being an important timber species for multip...
Monitoring patterns of mating and pollen dispersal in forest tree populations subjected to nature conservation is essential to understanding the dynamics of their reproductive processes and might be helpful in making management decisions aimed at con...
The remarkable species diversity of the genus Sorbus is a result of polyploidization and frequent hybridization between interacting species of different cytotypes. Moreover, hybridization is possible between several parental taxa. Gametophytic apomix...
The genetic diversity of natural populations is a key factor in the success of long-term ecosystem protection. We studied the genetic diversity and spatial genetic structure (SGS) in three endangered shrub birch (Betula humilis) populations using sev...
Advanced genetic and biotechnology tools will be required to realize the full potential of food and bioenergy crops. Given current regulatory concerns, many transgenic traits might never be deregulated for commercial release without a robust gene con...
The ongoing fragmentation of ash populations due to ash dieback requires an effective gene flow between individuals; thus, investigations on ash pollen transport are essential. In this study, comprehensive aerobiological field experiments at two seed...
15 January 2017
An electrostatic-barrier-forming window (EBW) was devised to capture airborne pollen, which can cause allergic pollinosis. The EBW consisted of three layers of insulated conductor wires (ICWs) and two voltage generators that supplied negative charges...
Fragmented ash populations due to ash dieback may lead to a limited gene flow and pollination success. Therefore, the viability of ash pollen plays a major role for the survival of the species. The extent to which the long-distance transport of polle...
Assessing pollen viability and anomalies is essential to optimize resources and improve hazelnut productivity. However, knowledge of pollen viability dynamics across cultivars and environments remains limited. This study applied impedance flow cytome...
The increase in honey fraud in the global market has highlighted the importance of pollen analysis in determining or confirming the botanical and geographical origins of honey. Numerous studies are currently underway to develop efficient and rapid me...
Alfalfa (Medicago sativa L.) is a vital global forage crop. Transgenic technology promises enhanced yield and quality, but requires rigorous environmental risk assessment, particularly regarding pollen-mediated gene flow, for which standardized proto...
Airborne pollen surveys provide information on various aspects of biodiversity and human health monitoring. Such surveys are typically conducted using the Burkard Multi-Vial Cyclone Sampler, but have to be technically optimized for eDNA barcoding. We...
The pollen tube is a key innovation of land plants that is essential for successful fertilisation. Its development and growth have been profusely studied in model organisms, but in spite of the economic impact of olive trees, little is known regardin...
Neonicotinoid insecticides have come under scrutiny for their potential role in honey bee declines. Additionally, reduced access to forage in agricultural areas creates the potential for risk interactions with these pesticides in regions critical for...
Allergic diseases are a major health concern worldwide. Pollens are important triggers for allergic rhinitis, conjunctivitis and asthma. Proteases released upon pollen grain hydration appear to play a major role in the typical immunological and infla...
The use of biotechnology for the genetic improvement of wheat (Triticum aestivum L.) has been hampered by its recalcitrance to standard transformation and regeneration protocols. Male gametes present a potentially useful option for introducing gene e...
Gametophytic factors mediate pollen-pistil interactions in maize (Zea mays L.) and play active roles in limiting gene flow among maize populations and between maize and teosinte. This study was carried out to identify proteins and investigate the mec...
Plantations of the valuable Chinese timber species, Chamaecyparis hodginsii, established by planting, primarily rely on seed orchards for propagation. Therefore, effective management of the genetic composition of these orchards is essential to ensure...
The argane tree (Argania spinosa L.) is a mostly self-incompatible species that must be cross-pollination. However, the cross-pollination is often insufficient to obtain a desirable fruit yield in the absence of compatibility between the orchard&rsqu...
Pollen development and its fertility are obligatory conditions for the reproductive success of flowing plants. Sucrose transporter 3 (OsSUT3) is known to be preferentially expressed and may play critical role in developing pollen. A 31-bp InDel was i...
Modern studies have shown that pollen has a certain role in the treatment of prostate-related diseases. In the present study, pollen polysaccharides from Chinese wolfberry (WPPs) were extracted by hot-water extraction and ethanol precipitation, furth...
The commercial application of genetically modified plants has been seriously impeded by public concern surrounding the potential risks posed by such plants to the ecosystem and human health. Previously, we have developed a ‘pollen- and seed-spe...
The sweet chestnut (Castanea sativa Mill.) is the only native species of this genus in Europe, where it faces various threats that are causing a severe decrease in populations, with the resulting loss of genetic diversity. In the Iberian Peninsula, i...
Background and Objectives: Polyploidisation and frequent hybridisation play an important role in speciation processes and evolutionary history and have a large impact on reproductive systems in the genus Crataegus. Reproductive modes in selected dipl...
Tomato flowers require vibrational stimulation for pollen release, but natural pollination is often limited in greenhouse environments. Airflow-assisted pollination offers a non-contact alternative; however, the vibration response of tomato plants un...
Limonium Mill. species present a polymorphic sexual system associated with flower polymorphisms like ancillary pollen and stigma, with sexual and/or apomictic reproduction. The aim of this study was to investigate the reproductive traits, test for au...
Background: Allergic rhinitis (AR) is a chronic condition that affects children’s quality of life. Studies show that adding bacterial lysates (BLs) to treatment can improve outcomes, but their effects on the immune system are still not fully un...
Many plant species depend on pollen flow to maximize reproduction and maintain genetic variability. Pollinators mediate this process, but distance between individuals can influence its benefits. Proximity may cause inbreeding depression, while extrem...
Introduction: 'Gold standard' in the diagnosis of atopic disease are skin prick tests and specific IgE evaluation. Well-established in vitro tests, such as the histamine release test, the leukotriens release test and the flow cytometric basophil acti...
Allocasuarina verticillata (Lam.) L.A.S. Johnson is a widespread species in south-eastern Australia providing vegetation cover, protecting fragile soils and providing food for birds. Understanding the effects of gene flow on the recruitment patterns,...
Agroforestry systems are essential in sustainable land use in the face of the growing global food demand and climate change. The southern region of Bahia, Brazil, is one of the places in the world where the tree species is particularly in abundance,...
White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generatin...
The development of double haploids (DHs) is a straightforward path for obtaining pure lines but has multiple bottlenecks. Among them is the determination of the optimal stage of pollen induction for androgenesis. In this work, we developed Microscan,...
Evaluation of multiple barriers contributing to reproductive isolation between sympatric plant species is key to understanding the mechanism of their coexistence; however, such investigations in biodiversity hotspots are still rare. In this study, we...
Background and Objectives: The contact zones of different refugial lineages, where mixing of genetic backgrounds leads to new gene combinations or pre-adaptations, represent hotspots of genetic diversity. The aim of the study was to compare patterns...
Interspecific hybridization of rapeseed is an important way to innovate breeding resources. This research used Brassica napus and Brassica rapa for artificial synthesis interspecific hybridization of F1. The F1 self-fruiting rate was particularly low...
27 August 2014
Air pollution is exacerbated near heavy traffic roads in cities. Air pollution concentration and composition vary by region and depend on urban-rural gradients. The aim of this study was to evaluate the distribution of air pollution in areas of vary...
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