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Volume 77
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Advances in Respiratory Medicine is published by MDPI from Volume 90 Issue 4 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Via Medica.
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Article

The Usefulness of CD203c Expression Measurement on Basophils after Activation with Grass Pollen and Dermatophagoides pteronyssinus Antigens. Preliminary Study

by
Olga Potapińska
1,*,
Elżbieta Górska
1,
Anna Zawadzka-Krajewska
2,
Marek Kulus
2,
Maria Wąsik
1 and
Urszula Demkow
1
1
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Warsaw Medical University, Marszałkowska 24, 00-576 Warsaw, Poland
2
Department of Pediatric Pneumonology and Allergology, Warsaw Medical University, Marszałkowska 24, 00-576 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Adv. Respir. Med. 2009, 77(2), 138-144; https://doi.org/10.5603/ARM.27827
Submission received: 26 June 2008 / Revised: 16 March 2009 / Accepted: 16 March 2009 / Published: 16 March 2009

Abstract

Introduction: 'Gold standard' in the diagnosis of atopic disease are skin prick tests and specific IgE evaluation. Well-established in vitro tests, such as the histamine release test, the leukotriens release test and the flow cytometric basophil activation test can be very helpful in diagnostics, especially when the skin prick test is contraindicated. The aim of this study was to evaluate the usefulness of antigen CD203c expression, as a marker of basophil activation by grass pollen or D. pteronyssinus antigens. Material and Methods: Peripheral blood from 13 allergic patients and nine healthy volunteers was analysed. Basophils activation was measured by the breakdown of antigen CD203c expression with Allergenicity Kit (Beckman Coulter), using Cytomics FC 500 flow cytometer (Beckman Coulter). Results: The sensitivity was 92,3% and specificity of test was 100%. 50.95 ± 15.7% of basophils (median 49.7%, 1.91–72.42%) were activated after grass pollen stimulation in atopic patients sensitised to this allergen, in comparison to 1.91% (0.00–7.96%) in control patients (p = 0.002). The percentage of activated basophils after D. pteronyssinus antigens stimulation was 40.6 ± 25.2% in allergic patients, compared to only 2.51 ± 1 96% of basophils from non-atopic controls (p = 0.0003). Basophils from 21 patients responded after anti-IgE stimulation (44.1 ± 18.9%), and none of the analysed samples was activated after PBS stimulation (2.03 ± 1.19%, p < 0.0001). Conclusions: These results demonstrate that basophil activation test based on antigen CD203c expression is very accurate in the diagnosis of atopic diseases.
Keywords: CD203c; basophils; atopic disease; grass pollen; D. pteronyssinus CD203c; basophils; atopic disease; grass pollen; D. pteronyssinus

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MDPI and ACS Style

Potapińska, O.; Górska, E.; Zawadzka-Krajewska, A.; Kulus, M.; Wąsik, M.; Demkow, U. The Usefulness of CD203c Expression Measurement on Basophils after Activation with Grass Pollen and Dermatophagoides pteronyssinus Antigens. Preliminary Study. Adv. Respir. Med. 2009, 77, 138-144. https://doi.org/10.5603/ARM.27827

AMA Style

Potapińska O, Górska E, Zawadzka-Krajewska A, Kulus M, Wąsik M, Demkow U. The Usefulness of CD203c Expression Measurement on Basophils after Activation with Grass Pollen and Dermatophagoides pteronyssinus Antigens. Preliminary Study. Advances in Respiratory Medicine. 2009; 77(2):138-144. https://doi.org/10.5603/ARM.27827

Chicago/Turabian Style

Potapińska, Olga, Elżbieta Górska, Anna Zawadzka-Krajewska, Marek Kulus, Maria Wąsik, and Urszula Demkow. 2009. "The Usefulness of CD203c Expression Measurement on Basophils after Activation with Grass Pollen and Dermatophagoides pteronyssinus Antigens. Preliminary Study" Advances in Respiratory Medicine 77, no. 2: 138-144. https://doi.org/10.5603/ARM.27827

APA Style

Potapińska, O., Górska, E., Zawadzka-Krajewska, A., Kulus, M., Wąsik, M., & Demkow, U. (2009). The Usefulness of CD203c Expression Measurement on Basophils after Activation with Grass Pollen and Dermatophagoides pteronyssinus Antigens. Preliminary Study. Advances in Respiratory Medicine, 77(2), 138-144. https://doi.org/10.5603/ARM.27827

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