764 Results Found

Sex-specific information is crucial for sturgeon culture, conservation, and fisheries management. However, identifying their sex is difficult outside the spawning season. Two recently identified female-specific loci (AllWSex2 and SSM4) are conserved...

Detecting IgE sensitizations in the serum of allergic dogs is commonly performed using allergen extracts, but these are difficult to standardize. This article details the development and validation of the Pet Allergy Xplorer (PAX; Nextmune, Stockholm...

Cronobacter sakazakii is associated with the ingestion of contaminated reconstituted powdered infant formula (PIF), resulting in necrotizing enterocolitis, sepsis and meningitis in neonatal infants. Potential virulence determinants include the variab...

Determination of poliovirus-neutralizing antibodies is an important part of clinical studies of poliovirus vaccines, epidemiological surveillance and seroprevalence studies that are crucial for global polio eradication campaigns. The conventional neu...

In this study, a rapid and sensitive immunoassay method has been established based on calibration curve implanted enzyme-linked immunosorbent assay (C-ELISA) for the simultaneously quantitative determination of aflatoxin B1, deoxynivalenol and zearal...

This study describes the development of a species determination/DNA quantification system called Pleo Qplex and an individual identification STR multiplex called Pleo STRplex using Panthera leo samples. Pleo Qplex enables us to measure the quantity o...

Recently, a multiplex PCR-based titration (MPBT) assay was developed for simultaneous determination of infectious titers of all three Sabin strains of the oral poliovirus vaccine (OPV) to replace the conventional CCID50 assay, which is both time-cons...

The biogeochemical cycling of nitrogen (N) in natural waterbodies, ranging from freshwaters to estuaries and seawater, is fundamental to the health of aquatic ecosystems. Anthropogenic pressures (agricultural runoff, atmospheric deposition, and waste...

Most of the current serological diagnosis of pertussis is based on pertussis toxin (PT) IgG antibodies and does not differentiate between vaccination and infection-induced antibodies. PT is included in all of acellular pertussis vaccines available in...

Background/Objectives: Bronchial wash for microbiological cultivation and/or multiplex polymerase chain reaction (PCR) is often routinely performed during bronchoscopy independent of the indication for bronchoscopy. This study aimed to determine and...

This study established a model for price determinants with the combination of the GIS technique and spatial regression model based on the parcel prices of multiplex houses in an effort to integrate and utilize spatial data and choose a suitable model...

The aim of the study was to demonstrate the influence of target gene and amplification product length on the performance of fetal gender determination systems using maternal plasma. A total of 40 pairs of plasma DNA samples from pregnant women and ge...

Background/Objectives: Sensitization to the carbohydrate antigen α-Gal is associated with allergic reactions against different types of food that contain α-Gal (e.g., mammalian meat). This form of allergy is termed α-Gal syndrome (A...

The article reviews the current status of label-free integrated optical biosensors focusing on the evolution over the years of their analytical performance. At first, a short introduction to the evanescent wave optics is provided followed by detailed...

Robust and sensitive ultrahigh-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) combined with the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was applied for the detection of seven Alternaria t...

Febrile seizure (FS) is a common benign seizure disorder of young children. Although upper respiratory tract infection is the cause of fever in most episodes of FS, studies to identify respiratory viruses using a multiplex polymerase chain reaction (...

Campylobacter jejuni and Campylobacter coli are the predominant thermophilic species responsible for foodborne gastroenteritis worldwide. Elevated resistance to certain antibiotics was observed due to antimicrobial therapy in farm animals and humans,...

Multiplexed single-photon sources can produce indistinguishable single photons with high probability in near-perfect spatial modes. Such systems, realized with optical elements having losses, can be optimized—that is, both the optimal number of...

Changes in the limits of detection (LODs) for a multiplex lateral flow immunoassay (LFIA) caused by different locations of the binding zone on the test strips were studied. Due to the non-equilibrium conditions of the immune reactions in LFIAs, their...

The individuals of real-world networks participate in various types of connections, each forming a layer in multiplex networks. Link prediction is an important problem in multiplex network analysis owing to its wide range of practical applications, s...

Next-generation sequencing (NGS) tools have importantly helped the classification of myelodysplastic syndromes (MDS), guiding the management of patients. However, new concerns are under debate regarding their implementation in routine clinical practi...

With the development of global navigation satellite systems (GNSS), multiple signals modulated on different sub-carriers are needed to provide various services and to ensure compatibility with previous signals. As an effective method to provide diver...

Reliable cytopathological diagnosis requires new methods and approaches for the rapid and accurate determination of all cell types. This is especially important when the number of cells is limited, such as in the cytological samples of fine-needle bi...

The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of clinical tests composed of multiple biomarkers. We investigated the diagnostic accuracy of the two multiplex...

Mycoviruses are viruses that naturally infect and replicate in fungi. They are widespread in all major fungal groups including plant and animal pathogenic fungi. Several dsRNA mycoviruses have been reported in Aspergillus fumigatus. Multiplex polymer...

To date, many fluorescence- and gel-based multiplex polymerase chain reaction (PCR) assays have been developed for the simultaneous detection of multiple infectious agents of respiratory disease in poultry. However, PCR assays are not available for o...

Bacterial meningitis infection is a leading global health concern for which rapid and accurate diagnosis is essential to reduce associated morbidity and mortality. Loop-mediated isothermal amplification (LAMP) offers an effective low-cost diagnostic...

This study proposes a multiplexed weak waist-enlarged fiber taper (WWFT) curvature sensor and its rapid fabrication method. Compared with other types of fiber taper, the proposed WWFT has no difference in appearance with the single mode fiber and has...

There is a need to identify the species of similar types of fish, especially those that are commercially sold. Particularly, the price of tuna varies depending on its type, which is difficult to determine as they are sold in cut or processed forms. T...

Management and prognosis of disease requires the accurate determination of specific biomarkers indicative of normal or disease-related biological processes or responses to therapy. Moreover since multiple determinations of biomarkers have demonstrate...

Vulvovaginal candidiasis (VVC) is a prevalent condition affecting women worldwide. This study aimed to develop a rapid qPCR assay for the accurate identification of VVC etiological agents and reduced azole susceptibility. One hundred and twenty nine...

Staphylococcal enterotoxins (SEs) are major contributors to foodborne intoxications. Reliable detection methods for SEs are essential to maintain food safety and protect public health. Since the heat-stable toxins also exert their toxic effect in the...

Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we p...

Areca Palm Velarivirus 1 (Velarivirus arecae, APV1), Areca palm necrotic ringspot virus (Arepavirus arecae, ANRSV), and Areca palm necrotic spindle-spot virus (Arepavirus arecamaculatum, ANSSV) are major viral pathogens that cause significant economi...

The prevalence of (TDF)-induced Acute Kidney Injury (AKI) in Africa is a concern, given the widespread use of Tenofovir disoproxil fumarate (TDF) for Human Immunodeficiency Virus (HIV) therapy. Current tests used to detect AKI are based on increased...

Precision nitrogen (N) management requires an accurate and timely in-season assessment of crop N status. The proximal fluorescence sensor Multiplex®3 is a promising tool for monitoring crop N status. It performs a non-destructive estimation of pl...

This paper describes an improved version of a sampling-based bridge designed for impedance calibration. Based on the original concept of the new multiplexer, which constitutes a key element of the improved impedance bridge, we propose a new procedure...

Multiplexed biosensing methods for simultaneously detecting multiple biomolecules are important for investigating biological mechanisms associated with physiological processes, developing applications in life sciences, and conducting medical tests. T...

The Time Division Multiple Access (TDMA) scheme has been proposed as the one which assures real-time messaging in a networking system. In the TDMA scheme, a networking system operates based on time slots which are defined as discrete time units. Some...

Background: The recent emergence of distinct and highly successful SARS-CoV-2 lineages has substantial implications for individual patients and public health measures. While next-generation-sequencing is routinely performed for surveillance purposes,...

This work reports the thermal modeling and characterization of a thyristor. The thyristor is used in a 6.5-MW generator excitation bridge. Temperature measurements are performed using fiber Bragg grating (FBG) sensors. These sensors have the benefits...

Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow a complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due to the...

The SARS-CoV-2 pandemic ignited global efforts to rapidly develop testing, therapeutics, and vaccines. However, the rewards of these efforts were slow to reach many low- to middle-income countries (LMIC) across the African continent and globally. The...

We report a high-speed (~2 kHz) dynamic multiplexed fiber Bragg grating (FBG) sensor interrogation using a wavelength-swept laser (WSL) with a polygon-scanner-based wavelength filter. The scanning frequency of the WSL is 18 kHz, and the 10 dB scann...

We propose in this paper a biosensor scheme based on coupled plasmon-waveguide resonance (CPWR) excited fluorescence spectroscopy. A symmetrical structure that offers higher surface electric field strengths, longer surface propagation lengths and dep...

The molecular fingerprinting methods used to evaluate soil microbial diversity could also be used as effective biosensors for the purposes of monitoring ecological soil status. The biodiversity of microorganisms is a relevant index of soil activity a...

The signal-to-noise ratio (SNR) and driving levels of capacitive touch sensors determine the applicability of these sensors to thinner displays and sensor-integrated modules. The simultaneous driving technique has been widely applied to capacitive to...

The Herpotrichiellaceae family is an important group of dematiaceous filamentous fungi, associated with a variety of pathogenic fungal species causing chromoblastomycosis (CBM) and phaeohyphomycosis (PHM), both with polymorphic clinical manifestation...

Non-O157 Shiga toxin-producing E. coli (STEC) can cause outbreaks that have great economic and health impact. Since the implementation of STEC screening in Alberta in 2018, it is also essential to have a molecular serotyping method with faster turnar...

In this study, we report a multiplexed platform for the simultaneous determination of five marine toxins. The proposed biosensor is based on a disposable electrical printed (DEP) microarray composed of eight individually addressable carbon electrodes...