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1,546 Results Found

  • Article
  • Open Access
15 Citations
5,016 Views
11 Pages

Influence of Chymosin on Physicochemical and Hydrolysis Characteristics of Casein Micelles and Individual Caseins

  • Chun-Chi Chen,
  • Liang-Yu Chen,
  • Wen-Tai Li,
  • Ken-Lin Chang,
  • Meng-I Kuo,
  • Chao-Jung Chen and
  • Jung-Feng Hsieh

1 October 2021

The effects of chymosin on the physicochemical and hydrolysis characteristics of casein micelles and individual caseins were investigated. Adding 0.03 units of chymosin/mL led to the casein micelles in skim milk coagulating after a 3 h incubation per...

  • Article
  • Open Access
2 Citations
2,051 Views
18 Pages

Low Demineralized Caseins to Replace Sodium Caseinate for Application in Whipped Creams

  • Khadija Florence Dabo,
  • Christine Chèné,
  • Sylvain Prevost,
  • Anne-Laure Fameau and
  • Romdhane Karoui

3 December 2024

Caseinate is commonly used in the dairy industry for its stabilizing properties. Its functionalities are largely due to its manufacturing process involving a high level of demineralization that induces casein precipitation. To address this, the resea...

  • Review
  • Open Access
2 Citations
1,931 Views
23 Pages

Heterologous Caseins: The Role of Phosphorylation in Their Functionality and How to Achieve It

  • Soledad Mora Vásquez,
  • Santiago García-Jacobo,
  • Guy A. Cardineau and
  • Silverio García-Lara

17 July 2025

Heterologous expression of caseins in non-mammalian systems offers a sustainable and scalable alternative for producing milk proteins, with potential applications in the food and biopharmaceutical industries. However, a significant challenge in these...

  • Article
  • Open Access
13 Citations
3,645 Views
12 Pages

Influence of Microbial Transglutaminase on Physicochemical and Cross-Linking Characteristics of Individual Caseins

  • Chun-Chi Chen,
  • Liang-Yu Chen,
  • Der-Sheng Chan,
  • Bang-Yuan Chen,
  • Hsien-Wei Tseng and
  • Jung-Feng Hsieh

2 September 2020

The effects of microbial transglutaminase (MTGase) cross-linking on the physicochemical characteristics of individual caseins were investigated. MTGase was used to modify three major individual caseins, namely, κ-casein (κ-CN), αS-c...

  • Review
  • Open Access
63 Citations
10,324 Views
36 Pages

21 February 2023

The milk of mammals is a complex fluid mixture of various proteins, minerals, lipids, and other micronutrients that play a critical role in providing nutrition and immunity to newborns. Casein proteins together with calcium phosphate form large collo...

  • Article
  • Open Access
24 Citations
8,907 Views
18 Pages

10 April 2021

Micellar casein and casein monomers in milk serum are in a dynamic equilibrium. At temperature below 15–20 °C a considerable amount of casein monomers, β-casein in particular, is released from the casein micelle into the aqueous serum phase. This stu...

  • Communication
  • Open Access
2 Citations
3,220 Views
10 Pages

1 June 2023

Membrane filtration, especially in combination with diafiltration, can affect the colloidal structure of casein micelles in milk and concentrated milks. The partial dissociation of casein proteins from the casein micelles into the serum phase has bee...

  • Article
  • Open Access
55 Citations
17,140 Views
15 Pages

17 December 2020

Acute-feeding and multiple-day studies have demonstrated that milk containing A2 β-casein only causes fewer symptoms of lactose intolerance (LI) than milk containing both A1 and A2 β-caseins. We conducted a single-meal study to evaluate the...

  • Article
  • Open Access
1 Citations
3,760 Views
11 Pages

Severe gastrointestinal symptoms (GIS) and food hypersensitivity are tightly associated in young individuals with autism spectrum disorders (ASD). Here, we explored the relationship of GIS (gastrointestinal severity index, ROMA IV criteria, Bristol s...

  • Article
  • Open Access
14 Citations
3,270 Views
15 Pages

27 January 2023

This study systematically investigated the differences in allergenicity of casein in cow milk (CM), goat milk (GM), camel milk (CAM), and mare milk (MM) from protein structures using bioinformatics. Primary structure sequence analysis reveals high se...

  • Article
  • Open Access
12 Citations
3,479 Views
15 Pages

Sensopeptidomic Kinetic Approach Combined with Decision Trees and Random Forests to Study the Bitterness during Enzymatic Hydrolysis Kinetics of Micellar Caseins

  • Dahlia Daher,
  • Barbara Deracinois,
  • Philippe Courcoux,
  • Alain Baniel,
  • Sylvie Chollet,
  • Rénato Froidevaux and
  • Christophe Flahaut

7 June 2021

Protein hydrolysates are, in general, mixtures of amino acids and small peptides able to supply the body with the constituent elements of proteins in a directly assimilable form. They are therefore characterised as products with high nutritional valu...

  • Article
  • Open Access
5 Citations
2,581 Views
13 Pages

A New AS-PCR Method to Detect CSN201 Allele, Genotyping at Ca-Sensitive Caseins Loci and Milk Traits Association Studies in Autochthonous Lazio Goats

  • Gianfranco Cosenza,
  • Sara Albarella,
  • Emanuele D’Anza,
  • Alessandra Iannuzzi,
  • Maria Selvaggi,
  • Mariagiulia Pugliano,
  • Tiziana Galli,
  • Giorgio Saralli,
  • Francesca Ciotola and
  • Vincenzo Peretti

9 January 2023

Calcium-sensitive caseins are the main protein component of milk. In the goat, they are encoded by three genes (CSN1S1, CSN2, and CSN1S2) located on chromosome 6. A high number of alleles has been discovered for these genes in the goat species, respo...

  • Feature Paper
  • Article
  • Open Access
6 Citations
3,054 Views
12 Pages

Ultrasound-Assisted Transglutaminase Catalysis of the Cross-Linking and Microstructure of αs-Casein, β-Casein and κ-Casein

  • Chun-Chi Chen,
  • Liang-Yu Chen,
  • Wen-Tai Li,
  • Ken-Lin Chang,
  • Hsien-Wei Tseng,
  • Bang-Yuan Chen,
  • Chao-Jung Chen and
  • Jung-Feng Hsieh

9 September 2021

The effects of ultrasonic treatment (UT)-assisted transglutaminase (TGase) catalysis on the physicochemical properties of individual αs-casein (αs-CN), β-casein (β-CN), and κ-casein (κ-CN) were investigated. After 60 min of incubation at 30 °C, αs-CN...

  • Article
  • Open Access
22 Citations
5,501 Views
18 Pages

26 February 2022

Electrodialysis with a bipolar membrane coupled to an ultrafiltration module (EDBM-UF) is a hybrid technology recently developed as an ecofriendly alternative to chemical acidification to produce casein and caseinate from skim milk. In this study, th...

  • Article
  • Open Access
39 Citations
7,979 Views
19 Pages

Antimicrobial Potential of Food Lactic Acid Bacteria: Bioactive Peptide Decrypting from Caseins and Bacteriocin Production

  • Stefano Nebbia,
  • Cristina Lamberti,
  • Giuliana Lo Bianco,
  • Simona Cirrincione,
  • Valerie Laroute,
  • Muriel Cocaign-Bousquet,
  • Laura Cavallarin,
  • Maria Gabriella Giuffrida and
  • Enrica Pessione

Lactic acid bacteria (LAB) potential in the food industry and in the biotechnological sector is a well-established interest. LAB potential in counteracting especially food-borne infections has received growing attention, but despite being a road full...

  • Article
  • Open Access
1 Citations
1,525 Views
17 Pages

Influence of the Casein Genotype on Goat Milk Bioactivity: An In Silico Analysis of the Casein Peptidome

  • Aram Y. Rubio-Reyes,
  • Iván Delgado-Enciso,
  • Eduardo Casas,
  • Estela Garza-Brenner and
  • Ana M. Sifuentes-Rincón

15 June 2025

Goat caseins are highly polymorphic proteins that affect milk functional properties. In this study, an in silico approach was employed to analyze the influence of goat casein allelic variants on the quantity and bioactivity potential of peptides rele...

  • Article
  • Open Access
9 Citations
3,160 Views
14 Pages

15 December 2022

The aim of the study was to apply the bioactive components casein hydrolysate and casein–iron chelate to yoghurt and to analyze their influence on product parameters. Casein was hydrolyzed with the non-commercial protease obtained from Yarrowia...

  • Article
  • Open Access
13 Citations
3,514 Views
16 Pages

1 September 2019

Non-covalent interactions and in vitro radical scavenging activities of the complexes formed by the commercial milk protein product caseinate and one of the two polyphenols (galangin and genistein) were assessed by the multi-spectroscopic techniques,...

  • Article
  • Open Access
20 Citations
5,859 Views
13 Pages

4 February 2023

Lactose maldigesters report an increase in abdominal pain due to the consumption of milk containing a mixture of A1 and A2 β-casein as compared to milk containing only A2 β-casein. Gastric transit affects gastrointestinal symptoms and rapid...

  • Article
  • Open Access
4 Citations
3,743 Views
12 Pages

Peptidomic Characterization and Amino Acid Availability after Intake of Casein vs. a Casein Hydrolysate in a Pig Model

  • Pablo Jiménez-Barrios,
  • Laura Sánchez-Rivera,
  • Daniel Martínez-Maqueda,
  • Yann Le Gouar,
  • Didier Dupont,
  • Beatriz Miralles and
  • Isidra Recio

21 February 2023

It is known that casein hydrolysis accelerates gastrointestinal transit in comparison to intact casein, although the effect of the protein hydrolysis on the composition of the digests is not fully understood. The aim of this work is to characterize,...

  • Article
  • Open Access
26 Citations
4,706 Views
18 Pages

A New Approach for Spontaneous Silver Ions Immobilization onto Casein

  • Oleksandra Pryshchepa,
  • Gulyaim N. Sagandykova,
  • Paweł Pomastowski,
  • Viorica Railean-Plugaru,
  • Anna Król,
  • Agnieszka Rogowska,
  • Agnieszka Rodzik,
  • Myroslav Sprynskyy and
  • Bogusław Buszewski

The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, the influence of light irradiation on the process was shown. In order to investigate the mechanism of meta...

  • Review
  • Open Access
149 Citations
53,962 Views
13 Pages

Milk Intolerance, Beta-Casein and Lactose

  • Sebely Pal,
  • Keith Woodford,
  • Sonja Kukuljan and
  • Suleen Ho

31 August 2015

True lactose intolerance (symptoms stemming from lactose malabsorption) is less common than is widely perceived, and should be viewed as just one potential cause of cows’ milk intolerance. There is increasing evidence that A1 beta-casein, a protein p...

  • Article
  • Open Access
6 Citations
4,912 Views
13 Pages

20 June 2024

Approximately 30% of milk protein is β-casein. We aimed to determine whether lactose maldigesters who chronically consumed two cups of A1/A2 milk (containing 75% A1 β-casein and 25% A2 β-casein) would adapt to have fewer intolerance sy...

  • Article
  • Open Access
1,089 Views
17 Pages

Functionalization of Sodium Caseinate for Production of Neat Films: Effects of Casein Crosslinking Induced by Heating at Alkaline pH or Light Exposure

  • Paolo D’Incecco,
  • Stefano Gerna,
  • Marta Sindaco,
  • Luisa Pellegrino,
  • Alberto Barbiroli,
  • Veronica Rosi and
  • Sara Limbo

8 August 2025

This study explored the functionalization of sodium caseinate (NaCas) using environmentally friendly approaches to improve the mechanical and structural properties of the derived films. NaCas functionalization was achieved through casein crosslinking...

  • Article
  • Open Access
9 Citations
4,922 Views
19 Pages

23 July 2019

Immunomodulatory nutraceuticals have garnered special attention due to their therapeutic potential for the amelioration of many chronic inflammatory conditions. Macrophages are key players in the induction, propagation and resolution of inflammation,...

  • Review
  • Open Access
7 Citations
5,022 Views
30 Pages

8 May 2025

The growing threat of antimicrobial resistance has intensified the search for alternative strategies to conventional antibiotics and preservatives. Casein-derived antimicrobial peptides (CDAMPs), generated through proteolysis, exhibit potent activity...

  • Article
  • Open Access
10 Citations
3,148 Views
15 Pages

29 November 2022

During the thermal processing of dairy products, the Maillard reaction occurs between milk proteins and lactose, resulting in the formation of various products including glycated proteins. In this study, lactose-glycated casein was generated through...

  • Article
  • Open Access
8 Citations
2,764 Views
12 Pages

Casein Microgels as Benzydamine Hydrochloride Carriers for Prolonged Release

  • Sofia Milenkova,
  • Bissera Pilicheva,
  • Yordanka Uzunova,
  • Temenuzhka Yovcheva and
  • Maria Marudova

11 February 2022

This research aims to investigate the properties of nano- and micro-sized casein hydrogels crosslinked by sodium tripolyphosphate as drug delivery systems. Benzydamine hydrochloride was chosen as a model hydrophilic drug. The gels were synthesized by...

  • Article
  • Open Access
13 Citations
2,913 Views
16 Pages

Durability and Strength Characteristics of Casein-Cemented Sand with Slag

  • Sung-Sik Park,
  • Seung-Wook Woo,
  • Sueng-Won Jeong and
  • Dong-Eun Lee

16 July 2020

Casein is often used as an eco-friendly wood adhesive. In this study, we used casein for soil cementation by mixing it with Jumunjin sand, sodium hydroxide (SH), and calcium hydroxide (CH) as a standard casein formula. The modified casein binder with...

  • Review
  • Open Access
3 Citations
2,220 Views
19 Pages

Casein-Based Biomaterials: Fabrication and Wound Healing Applications

  • Nikolay Estiven Gomez Mesa,
  • Krasimir Vasilev and
  • Youhong Tang

5 August 2025

Casein, the main phosphoprotein in milk, has a multifaceted molecular structure and unique physicochemical properties that make it a viable candidate for biomedical use, particularly in wound healing. This review presents a concise analysis of casein...

  • Article
  • Open Access
21 Citations
3,936 Views
14 Pages

Antibacterial Textile Based on Hydrolyzed Milk Casein

  • Kedafi Belkhir,
  • Caroline Pillon,
  • Aurélie Cayla and
  • Christine Campagne

6 January 2021

Antimicrobial textile structures are developed based on polypropylene (PP) and a natural material, hydrolyzed casein. The casein, from bovine milk, is subjected to acid hydrolysis in aqueous media, then blended into the PP matrix in the melt phase by...

  • Article
  • Open Access
19 Citations
4,125 Views
17 Pages

Flammability Characteristics and Mechanical Properties of Casein Based Polymeric Composites

  • Hanbin Lee,
  • Nam Kyeun Kim,
  • Daeseung Jung and
  • Debes Bhattacharyya

12 September 2020

Even though casein has an intrinsic potential ability to act as a flame retardant (FR) additive, the research regarding the FR performance of casein filled polymeric composites has not been thoroughly conducted. In the present work, two commercial ca...

  • Article
  • Open Access
2 Citations
2,255 Views
16 Pages

6 November 2023

One of the ways to modify selected parameters of lime mortars is the use of biopolymers of animal origin, such as bone glue, skin glue, bovine blood, eggs, and casein. These are protein-based biopolymers. Casein is an example of an organic polymer pr...

  • Article
  • Open Access
3 Citations
2,489 Views
20 Pages

The Influence of Casein Coatings on the Corrosion Behavior of Mg-Based Alloys

  • Aneta Kania,
  • Katarzyna Cesarz-Andraczke,
  • Zbigniew Brytan,
  • Łukasz Reimann and
  • Paulina Smolarczyk

14 February 2022

This article discusses the influence of conversion casein coatings with a thickness of about 20 µm on the structure and the corrosion behavior of two magnesium alloys: MgCa2Zn1 and MgCa2Zn1Gd3. Casein is a protein that, along with whey protein,...

  • Article
  • Open Access
1,079 Views
15 Pages

A Preliminary Investigation on the Thermal Behavior of Polysaccharides-Modified Casein

  • Maria R. Ricciardi,
  • Marco Russo,
  • Vincenza Antonucci,
  • Lorena Affatato and
  • Antonio Langella

The effective use of natural casein-based adhesives requires the reduction of shrinkage phenomena associated with the evaporation of water, which is largely used for preparation. After the procedure optimization of a casein natural glue by aid of an...

  • Article
  • Open Access
26 Citations
1 Views
5 Pages

Modulation of Mice Fecal Microbiota by Administration of Casein Glycomacropeptide

  • Qingsen Chen,
  • Jinyi Cao,
  • Yuchen Jia,
  • Xueji Liu,
  • Yali Yan and
  • Guangchang Pang

21 December 2012

Casein glycomacropeptide (GMP) is known to promote the in vitro growth of Bifidobacteria and Lactobacilli. In this paper, we used conventional culture techniques and fluorescent in situ hybridization (FISH) techniques to investigate the effect of cas...

  • Article
  • Open Access
34 Citations
6,082 Views
18 Pages

25 May 2019

Multilayer films containing α- and β-casein and polypeptides, poly-L-lysine (PLL), and poly-L-arginine (PLArg) were formed by the layer-by-layer technique and Fourier Transform InfraRed spectroscopy with Attenuated Total Reflection (FTIR-A...

  • Article
  • Open Access
3 Citations
3,163 Views
17 Pages

Analysis of the Frequency of the A1 and A2 Alleles in the Beta-Casein Gene and the A, B and E Alleles in the Kappa-Casein Gene in Local Cattle Breeds: Polish Red and Polish White-Backed

  • Wioletta Sawicka-Zugaj,
  • Witold Chabuz,
  • Joanna Barłowska,
  • Sebastian Mucha,
  • Karolina Kasprzak-Filipek and
  • Agnieszka Nowosielska

28 February 2025

In view of the threat to local breeds resulting from intensive animal production, many studies are conducted in search of arguments confirming their importance in food production. In the case of milk production, not only is its quantity important, bu...

  • Article
  • Open Access
16 Citations
4,574 Views
24 Pages

Antiseptic-Loaded Casein Hydrogels for Wound Dressings

  • Leonor Vasconcelos Garcia,
  • Diana Silva,
  • Maria Madalena Costa,
  • Henrique Armés,
  • Madalena Salema-Oom,
  • Benilde Saramago and
  • Ana Paula Serro

Chronic wound treatment accounts for a substantial percentage of the medical expenses worldwide. Improving and developing novel wound care systems can potentially help to handle this problem. Wound dressings loaded with antiseptics may be an importan...

  • Article
  • Open Access
14 Citations
2,831 Views
18 Pages

The Study of Zinc Ions Binding to αS1-, β- and κ-Casein

  • Agnieszka Rodzik,
  • Paweł Pomastowski,
  • Viorica Railean-Plugaru,
  • Myroslav Sprynskyy and
  • Bogusław Buszewski

30 October 2020

The presented studies focused on the specificity binding of particular casein fractions: αS1-, β- and κ-casein (αS1CN, βCN, κCN), with zinc ions. The binding mechanism was determined by kinetic modeling using results...

  • Article
  • Open Access
361 Views
13 Pages

Enhanced Rehydration of Micellar Casein Powder: Effects of Electrodialysis Treatment

  • Kerong Wang,
  • Yun Chen,
  • Xuhui Fan,
  • Shengbo Yu,
  • Yang Song,
  • Shuang Wang,
  • Weibo Zhang,
  • Pengjie Wang,
  • Shumin Wang and
  • Zhishen Mu
  • + 1 author

5 December 2025

The poor rehydration capacity of micellar casein (MC) powder due to the tightly cross-linked structure formed by colloidal calcium phosphate (CCP) limits its potential applications. This study aimed to improve the rehydration properties of MC powder...

  • Article
  • Open Access
8 Citations
3,362 Views
14 Pages

6 February 2022

In this study, milk protein casein was glycated by oligochitosan through the catalysis of transglutaminase (TGase) and then hydrolyzed by trypsin. The obtained glycated casein hydrolysates (GCNH) were assessed for their anti-inflammatory activities,...

  • Review
  • Open Access
14 Citations
5,182 Views
17 Pages

13 September 2024

Casein is among the most abundant proteins in milk and has high nutritional value. Casein’s interactions with polysaccharides, polyphenols, and metal ions are important for regulating the functional properties and textural quality of dairy food...

  • Article
  • Open Access
4 Citations
1,564 Views
13 Pages

Casein Functionalization Using High-Pressure Homogenization and Emulsifying Salts

  • Anthony Fuchs,
  • Danielle Stroinski,
  • Ashley Gruman and
  • Grace Lewis

29 March 2025

In milk, casein proteins orientate themselves into spherical micellar structures with hydrophobic casein subtypes concentrated in the core, while hydrophilic casein subtypes populate the exterior. Previous research demonstrated that milk with the add...

  • Article
  • Open Access
15 Citations
3,280 Views
19 Pages

Bayesian Analysis of the Association between Casein Complex Haplotype Variants and Milk Yield, Composition, and Curve Shape Parameters in Murciano-Granadina Goats

  • María Gabriela Pizarro Inostroza,
  • Francisco Javier Navas González,
  • Vincenzo Landi,
  • Jose Manuel León Jurado,
  • Juan Vicente Delgado Bermejo,
  • Javier Fernández Álvarez and
  • María del Amparo Martínez Martínez

10 October 2020

Considering casein haplotype variants rather than SNPs may maximize the understanding of heritable mechanisms and their implication on the expression of functional traits related to milk production. Effects of casein complex haplotypes on milk yield,...

  • Article
  • Open Access
2 Citations
2,901 Views
12 Pages

3 July 2023

Micellar casein fibers of defined size and internal structure can be produced by the extrusion of cold-renneted casein micelles into a warm, calcium-rich coagulation bath. Calcium phosphate contacts within the casein matrix are important for fiber st...

  • Article
  • Open Access
4 Citations
3,097 Views
19 Pages

Wet-Spun Chitosan–Sodium Caseinate Fibers for Biomedicine: From Spinning Process to Physical Properties

  • Hazel Peniche,
  • Ivy Ann Razonado,
  • Pierre Alcouffe,
  • Guillaume Sudre,
  • Carlos Peniche,
  • Anayancy Osorio-Madrazo and
  • Laurent David

1 February 2024

We designed and characterized chitosan–caseinate fibers processed through wet spinning for biomedical applications such as drug delivery from knitted medical devices. Sodium caseinate was either incorporated directly into the chitosan dope or a...

  • Article
  • Open Access
10 Citations
3,006 Views
16 Pages

Microscopical Evaluation of the Effects of High-Pressure Processing on Milk Casein Micelles

  • Sergio O. Serna-Hernandez,
  • Zamantha Escobedo-Avellaneda,
  • Rebeca García-García,
  • Magdalena de Jesús Rostro-Alanis and
  • Jorge Welti-Chanes

24 October 2022

The effect of different high-pressure processing (HPP) treatments on casein micelles was analyzed through scanning electron microscopy (SEM) and a particle size distribution analysis. Raw whole and skim milk samples were subjected to HPP treatments a...

  • Article
  • Open Access
16 Citations
3,864 Views
11 Pages

Changes in Metabolites from Bovine Milk with β-Casein Variants Revealed by Metabolomics

  • Zhongwang Lv,
  • Hui Liu,
  • Yongxin Yang,
  • Dengpan Bu,
  • Changjiang Zang,
  • Kailun Yang,
  • Xiong Yu and
  • Jiaqi Wang

30 May 2020

β-casein is a primary protein in milk, and its variants have been associated with changes in the protein content of bovine milk. However, there has been little research focused on the effects of β-casein variants on milk metabolites. In the...

  • Communication
  • Open Access
3 Citations
2,821 Views
10 Pages

No Glycation Required: Interference of Casein in AGE Receptor Binding Tests

  • Hannah E. Zenker,
  • Malgorzata Teodorowicz,
  • Harry J. Wichers and
  • Kasper A. Hettinga

9 August 2021

For the determination of the binding of heated cow’s milk whey proteins such as β-lactoglobulin to the receptors expressed on immune cells, inhibition ELISA with the soluble form of the receptor for advanced glycation end products (sRAGE) and scaveng...

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