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615 Results Found

  • Review
  • Open Access
7 Citations
6,092 Views
16 Pages

Bacterial Cellulose Production in Co-Culture Systems: Opportunities, Challenges, and Future Directions

  • Dheanda Absharina,
  • Filemon Jalu Nusantara Putra,
  • Chiaki Ogino,
  • Sándor Kocsubé,
  • Csilla Veres and
  • Csaba Vágvölgyi

Bacterial cellulose (BC), a nanostructured biopolymer produced by Komagateibacter spp., exhibits remarkable mechanical strength, purity, and biocompatibility, making it highly attractive for applications in biomedicine, food, and sustainable material...

  • Review
  • Open Access
12 Citations
5,624 Views
29 Pages

23 February 2023

Bacterial co-culture studies using synthetic gut microbiomes have reported novel research designs to understand the underlying role of bacterial interaction in the metabolism of dietary resources and community assembly of complex microflora. Since la...

  • Article
  • Open Access
1 Citations
1,653 Views
21 Pages

An UHPLC-HRMS-Based Untargeted Metabolomics Approach to Explore the Effects of Bacterial Endophyte Co-Culture on Alkanna tinctoria (L.) Tausch Cell Suspension Metabolome

  • Elodie Bossard,
  • Adrien Cousy,
  • Antonio Grondin,
  • Nikolaos Tsafantakis,
  • Angélique Rat,
  • Nektarios Aligiannis,
  • Anne Willems,
  • Laetitia Cattuzzato,
  • Thien Nguyen and
  • Nikolas Fokialakis

Colonization of plant tissues by bacterial endophytes might lead to qualitative and quantitative changes in secondary metabolites (SMs). In this work, in vitro co-culture experiments were performed using cell suspensions of the medicinal plant Alkann...

  • Article
  • Open Access
1 Citations
1,624 Views
18 Pages

Transparent 3-Layered Bacterial Nanocellulose as a Multicompartment and Biomimetic Scaffold for Co-Culturing Cells

  • Karla Pollyanna Vieira de Oliveira,
  • Michael Yilma Yitayew,
  • Ana Paula Almeida Bastos,
  • Stefanie Cristine Nied Mandrik,
  • Luismar Marques Porto and
  • Maryam Tabrizian

Three-dimensional (3D) cell culture models are widely used to provide a more physiologically relevant microenvironment in which to host and study desired cell types. These models vary in complexity and cost, ranging from simple and inexpensive to hig...

  • Article
  • Open Access
30 Citations
3,606 Views
17 Pages

28 November 2021

Clubroot disease caused by the obligate parasite Plasmodiophora brassicae is a serious threat to cabbage production worldwide. Current clubroot control primarily relies on a fungicide, but this has a negative impact on the environment and the use of...

  • Article
  • Open Access
8 Citations
3,142 Views
14 Pages

14 April 2022

Fungal–bacterial co-culturing is a potential technique for the production of secondary metabolites with antibacterial activity. Twenty-nine fungal species were screened in a co-culture with carbapenem-resistant Klebsiella pneumoniae at differen...

  • Protocol
  • Open Access
4 Citations
4,368 Views
13 Pages

Co-Culture of Gut Bacteria and Metabolite Extraction Using Fast Vacuum Filtration and Centrifugation

  • Asha Guraka,
  • Richard Duff,
  • Joe Waldron,
  • Gyanendra Tripathi and
  • Ali Kermanizadeh

19 September 2024

This protocol describes a robust method for the extraction of intra and extracellular metabolites of gut bacterial mono and co-cultures. In recent years, the co-culture techniques employed in the field of microbiology have demonstrated significant im...

  • Article
  • Open Access
15 Citations
5,447 Views
13 Pages

Do Kombucha Symbiotic Cultures of Bacteria and Yeast Affect Bacterial Cellulose Yield in Molasses?

  • Putu Virgina Partha Devanthi,
  • Katherine Kho,
  • Rizky Nurdiansyah,
  • Arnaud Briot,
  • Mohammad J. Taherzadeh and
  • Solmaz Aslanzadeh

28 August 2021

Bacterial cellulose (BC) is a valuable biopolymer typically observed in Kombucha with many potential food applications. Many studies highlight yeast’s roles in providing reducing sugars, used by the bacteria to grow and produce BC. However, whether y...

  • Article
  • Open Access
1 Citations
919 Views
18 Pages

Nitrogen Fertilizer Reduction in Rice–Eel Co-Culture System Improves the Soil Microbial Diversity and Its Functional Stability

  • Mengqian Ma,
  • Weiguang Lv,
  • Yu Huang,
  • Juanqin Zhang,
  • Shuangxi Li,
  • Naling Bai,
  • Haiyun Zhang,
  • Xianpu Zhu,
  • Chenglong Xu and
  • Hanlin Zhang

5 August 2025

The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term position...

  • Feature Paper
  • Article
  • Open Access
6 Citations
3,397 Views
14 Pages

High-Throughput Gel Microbeads as Incubators for Bacterial Competition Study

  • Trang Anh Nguyen-Le,
  • Xinne Zhao,
  • Michael Bachmann,
  • Philip Ruelens,
  • J. Arjan G. M. de Visser and
  • Larysa Baraban

12 March 2023

Bacteria primarily live in structured environments, such as colonies and biofilms, attached to surfaces or growing within soft tissues. They are engaged in local competitive and cooperative interactions impacting our health and well-being, for exampl...

  • Article
  • Open Access
1,697 Views
20 Pages

Various bacterial strains with nitrate-reducing capacity (NRC), such as Haemophilus, Actinomyces, and Neisseria, are known to promote NH3 production, control pH in the oral cavity, and inhibit the growth of aciduric bacteria. However, experimental ev...

  • Feature Paper
  • Article
  • Open Access
116 Citations
9,505 Views
19 Pages

Roles of Extracellular Polysaccharides and Biofilm Formation in Heavy Metal Resistance of Rhizobia

  • Natalia Nocelli,
  • Pablo C. Bogino,
  • Erika Banchio and
  • Walter Giordano

26 May 2016

Bacterial surface components and extracellular compounds, particularly flagella, lipopolysaccharides (LPSs), and exopolysaccharides (EPSs), in combination with environmental signals and quorum-sensing signals, play crucial roles in bacterial autoaggr...

  • Article
  • Open Access
5 Citations
3,268 Views
12 Pages

21 June 2024

Common carp (Cyprinus carpio) is a freshwater fish commonly farmed in rice fields, yet there were few studies on the intestinal functions and microbial community structure of common carp in the rice–carp co-culture system. Therefore, this study...

  • Article
  • Open Access
9 Citations
3,365 Views
13 Pages

The Effect of Dekkera bruxellensis Concentration and Inoculation Time on Biochemical Changes and Cellulose Biosynthesis by Komagataeibacter intermedius

  • Putu Virgina Partha Devanthi,
  • Ferren Pratama,
  • Katherine Kho,
  • Mohammad J. Taherzadeh and
  • Solmaz Aslanzadeh

15 November 2022

Bacterial Cellulose (BC) is a biopolymer with numerous applications. The growth of BC-producing bacteria, Komagataeibacter intermedius, could be stimulated by Dekkera bruxellensis, however, the effect on BC yield needs further investigation. This stu...

  • Article
  • Open Access
4 Citations
3,632 Views
21 Pages

Exploring the Potential of Polypropylene Fibers and Bacterial Co-Culture in Repairing and Strengthening Geopolymer-Based Construction Materials

  • Albert A. Griño,
  • Hannah Shane P. Soriano,
  • Michael Angelo B. Promentilla and
  • Jason Maximino C. Ongpeng

23 October 2023

This study explored self-healing in geopolymer mortar cured at ambient temperature using polypropylene fibers and bacterial co-cultures of Bacillus subtilis and Bacillus megaterium. Damage degree, compressive strength, ultrasonic pulse velocity (UPV)...

  • Article
  • Open Access
446 Views
22 Pages

18 January 2026

Plant biostimulatory effects of the green alga Chlorella sp. MACC-360, the Kocuria rhizophila FSP120 bacterial strain, and the combined inter-kingdom co-culture of the alga and bacterium were investigated using Solanum lycopersicum as a model plant g...

  • Article
  • Open Access
15 Citations
4,295 Views
15 Pages

A Microbial Co-Culturing System for Producing Cellulose-Hyaluronic Acid Composites

  • Marcello Brugnoli,
  • Ilaria Mazzini,
  • Salvatore La China,
  • Luciana De Vero and
  • Maria Gullo

In this study, a co-culture system combining bacterial cellulose (BC) producers and hyaluronic acid (HA) producers was developed for four different combinations. AAB of the genus Komagataeibacter sp. and LAB of the Lactocaseibacillus genus were used...

  • Article
  • Open Access
21 Citations
4,367 Views
14 Pages

Development of a Microalgae-Based Continuous Starch-to-Hydrogen Conversion Approach

  • Bettina Hupp,
  • Bernadett Pap,
  • Attila Farkas and
  • Gergely Maróti

Eukaryotic algae represent a highly heterogeneous group in terms of organization, lifestyle, and metabolic capabilities. Unicellular green microalgae are capable of biohydrogen production through direct and indirect photolysis as well as dark ferment...

  • Article
  • Open Access
6 Citations
2,664 Views
14 Pages

27 October 2023

Mycotoxins are secondary metabolites produced by a variety of fungi that contaminate animal food and feeds and are capable of inducing a wide range of toxicities. Predictive in vitro models represent valuable substitutes for animal experiments to ass...

  • Article
  • Open Access
9 Citations
3,110 Views
24 Pages

Chitosan versus Carboxymethyl Chitosan Cryogels: Bacterial Colonization, Human Embryonic Kidney 293T Cell Culturing and Co-Culturing

  • Andrey Boroda,
  • Yuliya Privar,
  • Mariya Maiorova,
  • Irina Beleneva,
  • Marina Eliseikina,
  • Anna Skatova,
  • Dmitry Marinin and
  • Svetlana Bratskaya

14 October 2022

The potential of chitosan and carboxymethyl chitosan (CMC) cryogels cross-linked with diglycidyl ether of 1,4-butandiol (BDDGE) and poly(ethylene glycol) (PEGDGE) have been compared in terms of 3D culturing HEK-293T cell line and preventing the bacte...

  • Brief Report
  • Open Access
5 Citations
3,304 Views
8 Pages

The search for new antimicrobial strategies is of major importance since there is a growing resistance of both bacteria and fungi to existing antimicrobials. Lipopeptides are promising and potent antimicrobial compounds. For translation into clinical...

  • Article
  • Open Access
8 Citations
2,937 Views
17 Pages

16 June 2022

Although barnyardgrass (Echinochloa crus-galli L.) is more competitive than rice (Oryza sativa L.) in the aboveground part, little is known about whether barnyardgrass is still competitive in recruiting endophytes and the root microbiota composition...

  • Article
  • Open Access
3 Citations
2,475 Views
14 Pages

14 August 2024

To evaluate the effects of filter-feeding fishes on water quality and bacterial community in the rice–crayfish coculture system, four different stocking densities of bighead carp (0, 500, 1000, 1500 ind./200 m2) were set up in rice–crayfi...

  • Article
  • Open Access
7 Citations
4,045 Views
26 Pages

Exploring Novel Fungal–Bacterial Consortia for Enhanced Petroleum Hydrocarbon Degradation

  • João Paulo Silva Monteiro,
  • André Felipe da Silva,
  • Rubens Tadeu Delgado Duarte and
  • Admir José Giachini

17 December 2024

Bioremediation, involving the strategic use of microorganisms, has proven to be a cost-effective alternative for restoring areas impacted by persistent contaminants such as polycyclic aromatic hydrocarbons (PAHs). In this context, the aim of this stu...

  • Communication
  • Open Access
3 Citations
2,081 Views
8 Pages

Salmonid rickettsial septicemia (SRS) is a critical sanitary problem in the Chilean aquaculture industry since it induces the highest mortality rate in salmonids among all infectious diseases. Piscirickettsia salmonis, a facultative intracellular bac...

  • Article
  • Open Access
27 Citations
4,655 Views
15 Pages

Advanced “Green” Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy

  • Sirina Zhantlessova,
  • Irina Savitskaya,
  • Aida Kistaubayeva,
  • Ludmila Ignatova,
  • Aizhan Talipova,
  • Alexander Pogrebnjak and
  • Ilya Digel

8 August 2022

Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequis...

  • Article
  • Open Access
17 Citations
5,122 Views
21 Pages

Probiotic bacteria modulate macrophage immune inflammatory responses, with functional cytokine responses determined by macrophage subset polarisation, stimulation and probiotic strain. Mucosal macrophages exhibit subset functional heterogeneity but a...

  • Article
  • Open Access
6 Citations
3,882 Views
15 Pages

Biohydrogen production from wastewater using eukaryotic green algae can be facilitated by appropriately selected bacterial partners and cultivation conditions. Two Chlorella algal species were chosen for these experiments, based on their robust growt...

  • Article
  • Open Access
13 Citations
5,834 Views
13 Pages

Investigation of Legionella Contamination in Bath Water Samples by Culture, Amoebic Co-Culture, and Real-Time Quantitative PCR Methods

  • Akiko Edagawa,
  • Akio Kimura,
  • Takako Kawabuchi-Kurata,
  • Shinichi Adachi,
  • Katsunori Furuhata and
  • Hiroshi Miyamoto

We investigated Legionella contamination in bath water samples, collected from 68 bathing facilities in Japan, by culture, culture with amoebic co-culture, real-time quantitative PCR (qPCR), and real-time qPCR with amoebic co-culture. Using the conve...

  • Article
  • Open Access
15 Citations
4,736 Views
14 Pages

Volatile Fatty Acid Production from Food Waste Leachate Using Enriched Bacterial Culture and Soil Bacteria as Co-Digester

  • Van Hong Thi Pham,
  • Jeongyoon Ahn,
  • Jaisoo Kim,
  • Sangbeom Lee,
  • Ingyu Lee,
  • Sungchul Kim,
  • Soonwoong Chang and
  • Woojin Chung

26 August 2021

The production of volatile fatty acids (VFAs) from waste stream has been recently getting attention as a cost-effective and environmentally friendly approach in mechanical–biological treatment plants. This is the first study to explore the use of a f...

  • Article
  • Open Access
12 Citations
3,881 Views
14 Pages

30 September 2022

Bacterial-fungal interactions are important in the functioning of natural ecosystems. We examined possible synergistic or antagonistic effects during the degradation of polycyclic aromatic hydrocarbons (PAHs) by a fungal–bacterial co-culture. B...

  • Article
  • Open Access
7 Citations
3,118 Views
16 Pages

Enterococcus faecalis Shields Porphyromonas gingivalis in Dual-Species Biofilm in Oxic Condition

  • Huan Chang Tan,
  • Gary Shun Pan Cheung,
  • Jeffrey Wen Wei Chang,
  • Chengfei Zhang and
  • Angeline Hui Cheng Lee

Aim: To develop a reproducible biofilm model consisting of Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) and to evaluate the interaction between the two bacterial species. Methodology: E. faecalis and P. gingivalis...

  • Article
  • Open Access
1,717 Views
16 Pages

Co-Culturing Bacillus Strains for Enhanced Antimicrobial Activity

  • Charlie Tran,
  • Russell S. Addison,
  • Ian E. Cock,
  • Xiaojing Chen and
  • Yunjiang Feng

8 September 2025

Background/Objectives: Bacillus probiotic mixtures have been used as a novel source of antimicrobial production. However, there is a need to emphasize the potential of co-cultured Bacillus strains and to understand the underlying mechanisms involved...

  • Article
  • Open Access
29 Citations
6,117 Views
16 Pages

Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture

  • Bathini Thissera,
  • Hani A. Alhadrami,
  • Marwa H. A. Hassan,
  • Hossam M. Hassan,
  • Fathy A. Behery,
  • Majed Bawazeer,
  • Mohammed Yaseen,
  • Lassaad Belbahri and
  • Mostafa E. Rateb

10 February 2020

Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, w...

  • Article
  • Open Access
13 Citations
6,360 Views
13 Pages

Exometabolomic Analysis of Cross-Feeding Metabolites

  • Andrea Lubbe,
  • Benjamin P. Bowen and
  • Trent Northen

4 October 2017

Microbial consortia have the potential to perform complex, industrially important tasks. The design of microbial consortia requires knowledge of the substrate preferences and metabolic outputs of each member, to allow understanding of potential inter...

  • Article
  • Open Access
6 Citations
3,661 Views
18 Pages

Enhanced Oil Recovery in a Co-Culture System of Pseudomonas aeruginosa and Bacillus subtilis

  • Dingyu Kang,
  • Hai Lin,
  • Qiang Li,
  • Nan Su,
  • Changkun Cheng,
  • Yijing Luo,
  • Zhongzhi Zhang and
  • Zhiyong Zhang

Microbial enhanced oil recovery (MEOR) is a promising technology for oil field extraction. This study investigated a co-culture system of Pseudomonas aeruginosa and Bacillus subtilis to increase MEOR efficacy. We analyzed bacterial growth, biosurfact...

  • Article
  • Open Access
3 Citations
2,723 Views
15 Pages

Kuratsuki bacteria enter the sake production process and affect the flavor and taste of sake. This study compared gene expression in the sake yeast Saccharomyces cerevisiae in co-culture with kuratsuki Kocuria to that in monoculture. Among the 5922 g...

  • Article
  • Open Access
34 Citations
8,647 Views
17 Pages

Investigation of Interspecies Interactions within Marine Micromonosporaceae Using an Improved Co-Culture Approach

  • Navid Adnani,
  • Emmanuel Vazquez-Rivera,
  • Srikar N. Adibhatla,
  • Gregory A. Ellis,
  • Doug R. Braun and
  • Tim S. Bugni

24 September 2015

With respect to bacterial natural products, a significant outcome of the genomic era was that the biosynthetic potential in many microorganisms surpassed the number of compounds isolated under standard laboratory growth conditions, particularly among...

  • Article
  • Open Access
3 Citations
2,367 Views
16 Pages

26 April 2025

A significant body of controlled laboratory research suggests different biological mechanisms by which the low-cost co-culture of seaweed and shrimp could improve sustainability whilst increasing income for the many poor pond farmers of South-East As...

  • Article
  • Open Access
5 Citations
3,152 Views
17 Pages

26 October 2023

Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (FOC), poses a serious threat to cucumber productivity. Compared to traditional chemical pesticides, biological control strategies have attracted more attention recently owing to their ef...

  • Brief Report
  • Open Access
1 Citations
1,779 Views
11 Pages

Clostridium perfringens enterotoxin (Cpe)-producing strains cause gastrointestinal infections in humans and account for the second-largest number of all foodborne outbreaks caused by bacterial toxins. The Cpe toxin is only produced during sporulation...

  • Article
  • Open Access
81 Citations
10,935 Views
20 Pages

Faecalibacterium prausnitzii is a major commensal bacterium in the human gut. It produces short-chain fatty acids that promote intestinal health. However, the bacterium is extremely oxygen-sensitive, making it difficult to develop as a probiotic. To...

  • Article
  • Open Access
2 Citations
3,807 Views
9 Pages

Effects of Escherichia coli and Proteus mirabilis on the Growth and Aggregation of Calcium Oxalate Crystal under Microaerobic Conditions

  • Krittaya Saelee,
  • Aroonlug Lulitanond,
  • Nattaya Sae-ung,
  • Vitoon Prasongwatana,
  • Patcharee Boonsiri and
  • Ratree Tavichakorntrakool

31 October 2022

Escherichia coli and Proteus mirabilis are common single- and polymicrobial urinary tract infections which can survive under various oxygen levels, including inside of stone matrices. Therefore, we aimed to investigate and compare the calcium oxalate...

  • Article
  • Open Access
2 Citations
2,120 Views
16 Pages

Interaction Between Lactic Acid Bacteria and Acetic Acid Bacteria in Sichuan Bran Vinegar: Impact on Their Growth and Metabolites

  • Jianlong Li,
  • Jie Wu,
  • Meiling Tu,
  • Xue Xiao,
  • Kaidi Hu,
  • Qin Li,
  • Ning Zhao,
  • Aiping Liu,
  • Xiaolin Ao and
  • Shuliang Liu
  • + 1 author

23 April 2025

Microbial interactions are essential for maintaining the stability and functionality of microbiota in fermented foods. In this study, representative strains of predominant lactic acid bacteria and acetic acid bacteria in Sichuan bran vinegar were sel...

  • Article
  • Open Access
34 Citations
5,218 Views
17 Pages

Microbial Electrochemical Treatment of Methyl Red Dye Degradation Using Co-Culture Method

  • Kalpana Sharma,
  • Soumya Pandit,
  • Abhilasha Singh Mathuriya,
  • Piyush Kumar Gupta,
  • Kumud Pant and
  • Dipak A. Jadhav

24 December 2022

Methyl red, a synthetic azo dye, was reported for not only being mutagenic but also its persistence has severe consequences on human health, such as cancer, alongside detrimental environmental effects. In the present study, the Pseudomonas putida OsE...

  • Article
  • Open Access
6 Citations
5,054 Views
19 Pages

Translation of novel inhalable therapies for respiratory diseases is hampered due to the lack of in vitro cell models that reflect the complexity of native tissue, resulting in many novel drugs and formulations failing to progress beyond preclinical...

  • Communication
  • Open Access
7 Citations
6,102 Views
13 Pages

Astrocytes Enhance Streptococcus suis-Glial Cell Interaction in Primary Astrocyte-Microglial Cell Co-Cultures

  • Jana Seele,
  • Roland Nau,
  • Chittappen K. Prajeeth,
  • Martin Stangel,
  • Peter Valentin-Weigand and
  • Maren Seitz

Streptococcus (S.) suis infections are the most common cause of meningitis in pigs. Moreover, S. suis is a zoonotic pathogen, which can lead to meningitis in humans, mainly in adults. We assume that glial cells may play a crucial role in host-pathoge...

  • Article
  • Open Access
7 Citations
3,509 Views
18 Pages

20 July 2023

Trichoderma spp. are widely used beneficial microbes in agricultural production; however, the improper carrier choice for Trichoderma agent preparation can alter the effectiveness of Trichoderma fungicides. In this study, the co-culture of four Trich...

  • Article
  • Open Access
34 Citations
5,603 Views
13 Pages

Elicitation of Antimicrobial Active Compounds by Streptomyces-Fungus Co-Cultures

  • Matthieu Nicault,
  • Ali Zaiter,
  • Stéphane Dumarcay,
  • Patrick Chaimbault,
  • Eric Gelhaye,
  • Pierre Leblond and
  • Cyril Bontemps

The bacteria of the genus Streptomyces and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard l...

  • Article
  • Open Access
8 Citations
2,304 Views
16 Pages

25 February 2024

Traditional rice production is often reliant on the unsustainable practice of utilizing intensive inputs in monoculture cropping systems. Alternatives fallow cover cropping and rice–fish coculture (RFC) offer promising solutions. However, the p...

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