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Open AccessArticle

Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture

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School of Computing, Engineering & Physical Sciences, University of the West of Scotland, Paisley PA1 2BE, UK
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Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia
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King Fahd Medical Research Centre, King Abdulaziz University, Jeddah 21589, Saudi Arabia
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Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, Egypt
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Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
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Department of Pharmacy, College of Pharmacy, Riyadh Elm University, Riyadh 11681, Saudi Arabia
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Laboratory of Soil Biology, University of Neuchatel, 2000 Neuchatel, Switzerland
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(2), 268; https://doi.org/10.3390/biom10020268
Received: 25 December 2019 / Revised: 3 February 2020 / Accepted: 6 February 2020 / Published: 10 February 2020
(This article belongs to the Section Natural and Bio-inspired Molecules)
Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment. View Full-Text
Keywords: Pantoea; Penicillium; pulicatin; antifungal; co-culture Pantoea; Penicillium; pulicatin; antifungal; co-culture
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Thissera, B.; Alhadrami, H.A.; Hassan, M.H.A.; Hassan, H.M.; Behery, F.A.; Bawazeer, M.; Yaseen, M.; Belbahri, L.; Rateb, M.E. Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture. Biomolecules 2020, 10, 268.

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