Search Results (72)

This article engages the concept of death as a revolutionary transformation within the contexts of mind uploading (MU) and Pierre Teilhard de Chardin’s theology of the Omega Point. The focus is not on the Omega Point itself as a conceptual endpoint, nor on what becomes of consciousness once it has been successfully uploaded into a digital substrate. To grasp the meaning of “death” within the context of MU, the article employs the notion of liminal speculative imagination to analyze death amid the liminality of biological mortality and digital immortality. It argues that death is more than just the end of life but rather as a transitional phase between different states of being. It contends that this liminal gap between life forms is an inherent aspect of existence that cannot be eliminated at a fundamental level. Instead of seeking to overcome death, the article suggests embracing the transformative nature of this liminality. In this view, death is seen as a natural and unavoidable part of life, serving as a mediator or catalyst for transitions into new forms of existence. Rather than focusing solely on achieving immortality, the article advocates for utilizing technology to facilitate evolution and advancement. By acknowledging the role of death in the process of transformation, particularly within the context of transhumanism, the article proposes that embracing liminality can lead to new possibilities for human evolution. This includes the potential emergence of transhuman life forms, such as those enabled by MU technologies. Full article

(1) Background: The survival and growth of startups in their early stages are negatively impacted by the lack of financial sustainability and scarce resources that entrepreneurs face during the first 5 years. This is known as the Valley of Death (VoD). This study seeks to identify key factors that influence the financial sustainability of startups during the VoD, which demands a significant amount of funding and government support. (2) Methods: A quantitative methodology was employed, based on a worldwide literature review that pointed out the variables of the object of study; the information collection process was conducted through a questionnaire applied to 352 entrepreneurs in an innovative ecosystem. This study empirically applies a structural equation model to determine the relationship between constructs. (3) Results: A comprehensive analysis of the results indicates that indicators such as positive sales performance, sufficient financial solvency to meet short- and long-term commitments, accurate pricing policies, and access to government and banking support are the primary factors affecting the sustainability of startups in the early stages. (4) Originality: This study provides original and relevant insights into the indicators that affect the financial sustainability of startups during the VoD and offers interesting insights for governments, institutions, and entrepreneurs to foster innovative ecosystems; it also contributes to the extant literature on early-stage entrepreneurial failures. Full article

Background: We explore how springs and their pastures are utilised by nomadic pastoralists and how they influence interchange and conflict between the custodians of the springs and the society of pastoralists in the broader region. Methods: The custodians of three spring locations in the Afar region of Ethiopia were interviewed to identify the advantages, and the challenges, of having springs on a pastoral estate. Results: The springs provide permanent water and health benefits, are used for cooking, and provide permanent pasture for livestock. In the Dobi Valley, the groundwater supporting the springs supports a population of the palm garayto (Hyphaene thebaica), which provides thatch and a liquor derived from the sap. The custodians of the springs are baaro-mara for their homelands and they welcome and provide access to the resources of the spring to Afar outsiders, gurro-mara. Recent invasions by the Somali Issa tribe have resulted in the deaths and displacement of Afar residents at Alalo-bad and Teo. The prickly shrub Prosopis juliflora has become another invasive threat with great impacts on the livelihood of the baaro-mara at two locations. Conclusions: Springs are oases where permanent water provides perennial resources in a semi-desert. However, the tenure of these precious havens can be subject to tribal contest and the resources of the springs have been recently undermined by invasion of a plant from another continent. The removal of the incipient population of Prosopis juliflora should be a priority before it proliferates further. Full article

This case report examines the correlation between hydration, weight variation, and perceived effort in a 43-year-old amateur athlete during a self-supported 81.5 km crossing of Death Valley, completed over 3 days with significant elevation changes. Studies have shown that a body mass loss greater than 2–3% can lead to an increased perception of effort and a decline in performance. Specifically, during passive and active heat exposures, the average body mass loss was found to be 1.4 ± 0.3% and 4.1 ± 0.7%, respectively. Salivary osmolarity has demonstrated a sensitivity of 86% and specificity of 91% in diagnosing dehydration of ≥ 2%, suggesting its potential as a non-invasive indicator of hydration status. The subject monitored their own body weight, hydration (via salivary osmolarity), and perceived effort using a rate of perceived exertion (RPE) scale. Nutritional intake included isocaloric meals and nutritional bars, and hydration was managed using water and a hydroelectrolytic solution. Key bioimpedance parameters were measured to assess body composition and hydration status. A progressive decrease in body weight correlated with an increase in perceived effort (RPE score) and salivary osmolarity. Resistance (Rx) remained stable, while reactance (Xc) showed a biphasic trend and was inversely correlated with the sodium/potassium ratio (NAK). There were significant linear correlations between perceived effort and both weight loss and salivary osmolarity, indicating that salivary osmolarity is a potential early predictor of these changes. The findings highlight a linear correlation between weight loss, perceived effort, and salivary osmolarity, suggesting that monitoring salivary osmolarity would be useful for the field assessment of hydration and exertion. Further research with larger populations is necessary to validate these observations. Full article

Startups often face uncertainty and the potential Valley of Death. To address these challenges, Sustainable Development Goals (SDGs) can serve as a starting point to identify business opportunities in markets driven by SDG-related issues. In this case, we propose using virtual reality (VR) technology as a “painkiller”. This study aims to explore VR use cases and their implications for startup business models and discover a problem–solution fit, i.e., identify the degree to which VR applications effectively address actual problems as “killer applications”. The study employs a customized customer discovery technique, an essential process in the Customer Development methodology. By exploring VR’s applicability in various domains such as entertainment, training, education, and tourism, this study unveils compelling use cases that align with SDG aspects, thus validating the hypothesis on startups’ potential to harness VR for SDG contributions. Furthermore, the study produces VR business model designs based on solutions to SDG-related problems. Thus, startups can function effectively as “painkillers”, offering solutions that are not only desired but urgently needed (problem–solution fit). The implications of these findings extend globally, underlining VR’s role in addressing SDG issues and offering practical insights for startup business model development that are applicable to broader technology adoptions. Full article

Previous hematologic and serum biochemistry reference interval (RI) values have been established for donkeys in various geographic regions, life-stages, or for specific donkey breeds. The last extensive investigation establishing RIs for adult donkeys in the United States (U.S.) was published over three decades ago. We aimed to establish updated robust RIs using a reference population of apparently healthy adult donkeys from across the U.S. Standard sized (n = 102), miniature (n = 17), and mammoth (n = 1) donkeys from four different states were enrolled, with 20% of the study population including donkeys captured directly from the wild in Death Valley National Park, CA. RIs were established in accordance with the American Society for Veterinary Clinical Pathology and Clinical Laboratory Standards Institute guidelines. The findings will assist practitioners with the interpretation of their complete blood count and biochemistry panel results in U.S. donkeys. This study also highlights a comparison of results for some important analytes in U.S. donkeys compared to U.S. horses and previously established donkey RIs. Full article

The Hindukush and Himalaya regions of Pakistan are chronically prone to several geological hazards such as landslides. Studying landslides in these regions is crucial for risk assessment and disaster management, as well as for determining the effects of adverse climatic conditions, infrastructure management, and increasing anthropogenic activities. High-relief mountains in these regions face severe challenges because of frequently occurring landslides and other natural hazards, especially during intensive rainfall seasons and seismic activity, which destroy infrastructure and cause injuries and deaths. Landslides in the Alpuri Valley (Hindukush) and the Neelum Valley (Himalaya) have been activated through high magnitude earthquakes, intensive rainfalls, snowfall, floods, and man-made activities. Landslide susceptibility mapping in these areas is essential for sustainable development as it enables proactive risk management, up-to-date decision-making, and effective responses to landslide hazards, ultimately safeguarding human lives, property, and the environment. In this study, the relative effect method was applied for landslide susceptibility modeling in both study areas to determine the capability to reduce the effects of landslides, and to improve the prediction accuracy of the method. The relative effect is a statistical model that has only been used for very limited time for landslide susceptibility with effective results. A total of 368 (Neelum Valley) and 89 (Alpuri Valley) landslide locations were identified, which were utilized to prepare the reliable landslide inventory using GIS. In order to evaluate the areas at risk for future landslides activities and determine their spatial relationship with landslide occurrences, the landslide inventory was developed with 17 landslide causative factors. These factors include slope gradient, slope aspect, geology, plan curvature, general curvature, profile curvature, elevation, stream power index, drainage density, terrain roughness index, distance from the roads, distance from the streams, distance from fault lines, normalized difference wetness index, land-use/land-cover, rainfall, and normalized difference vegetation index. Finally, the performance of the relative effect method was validated using the success and prediction curve rate. The AUC-validated result of the success rate curve in the Alpuri Valley is 74.75%, and 82.15% in the Neelum Valley, whereas, the AUC-validated result of the prediction rate curve of the model is 87.87% in the Alpuri Valley and 82.73% in the Neelum Valley. These results indicate the reliability of the model to produce a landslide susceptibility map, and apply it to other landslide areas. The model demonstrated a more effective result in the Alpuri Valley, having a smaller area. However, the results are also desirable and favorable in Neelum Valley, with it being a large area. It will assist in general landslide hazard management and mitigation, and further research studies related to future landslide susceptibility assessments in other parts of the region. Full article

The elderly are considered a high-risk group for severe outcomes and death from COVID-19 infection. Given the emergence of new COVID variants and the immunity provided by vaccines waning over time, booster doses of the vaccine have been advocated for those at risk to stay protected. This study aimed to determine the factors associated with hesitancy toward the second booster of the COVID-19 vaccine among the elderly residing in residential care homes. A cross-sectional study was conducted in 24 residential care homes in the Klang Valley using a face-to-face interview questionnaire. The study population included individuals aged 60 and above who had been fully vaccinated against COVID-19 up to the first booster dose. Second-booster hesitancy was assessed using the Oxford Vaccine Hesitancy Scale with seven items, the aggregate score of which ranges from seven to thirty-five; the higher the score, the greater the level of hesitancy. Multivariate linear regression was employed to determine factors associated with second-booster hesitancy, and a p-value < 0.05 was considered statistically significant. Data from 401 elderly individuals were included for analysis. The mean score of the Oxford Vaccine Hesitancy Scale was 21.6 ± 7.2. Predictors of second booster hesitancy were identified. Age, Indian ethnicity, being a recipient of the Sinovac vaccine as the first COVID-19 booster, experiencing the death of close friends or immediate family members following COVID-19 vaccination, and negative messages (indicating that taking a booster dose is harmful) from caregivers, friends, or family members were found to be associated with an increased second-booster-hesitancy score. Conversely, positive messages (indicating that taking a booster is helpful) from the government and caregivers, friends, or family members were identified as predictors associated with a reduction in the second-booster-hesitancy score. While vaccines effectively combat severe COVID-19, the majority of the elderly hesitate before taking the second booster. Their hesitancy, rooted in the perception of a low self risk and reliance on protection from the initial doses, emphasizes the need for intervention by relevant bodies. Taking into consideration the risk, albeit relatively low, of potentially serious side effects following COVID-19 vaccinations, it is imperative that transparent, appropriate, and positive messaging regarding booster vaccines, particularly in the context of the elderly from residential care homes, be available. Encouraging this high-risk group to embrace the second booster aligns with the goal of maximizing protection within the vulnerable elderly population. Full article

Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% of global infectious diseases and cause nearly 700,000 deaths every year. Their rapid increase and spread constitute a huge challenge for public health, highlighting the need for early detection during epidemics, to curtail the virus spread, and to enhance outbreak management. Here, we compared a standard quantitative polymerase chain reaction (RT-qPCR) and a direct RT-qPCR assay for the detection of Zika (ZIKV), Chikungunya (CHIKV), and Rift Valley Fever (RVFV) viruses from experimentally infected-mosquitoes. The direct RT-qPCR could be completed within 1.5 h and required 1 µL of viral supernatant from homogenized mosquito body pools. Results showed that the direct RT-qPCR can detect 85.71%, 89%, and 100% of CHIKV, RVFV, and ZIKV samples by direct amplifications compared to the standard method. The use of 1:10 diluted supernatant is suggested for CHIKV and RVFV direct RT-qPCR. Despite a slight drop in sensitivity for direct PCR, our technique is more affordable, less time-consuming, and provides a better option for qualitative field diagnosis during outbreak management. It represents an alternative when extraction and purification steps are not possible because of insufficient sample volume or biosecurity issues. Full article

Disasters caused by climate change are continuing around the world. Densely populated urban areas have been pushed to the forefront of global climate change, and ecological security has gradually become the focus of global attention. Whether it is predictable natural disasters, or it is long-term global warming, or any unexpected events, exploring green development that coexists with them, green urban environmental strategies will play an important role in urban development. We analyzed data from 31 sample cities in China, accurately quantified indicators through qualitative and quantitative analysis, and then explored the development status and related indicator systems of green cities in China by “Driving Force–State–Response” model. The results reveal that (1) economic factors are the fundamental and decisive factors for the development of green cities. The higher the level of economic development, the stronger the ability of urban development; we also revealed the indicators that are significantly correlated with the level of green city construction and clarified the important factors of green cities; (2) technological factors are crucial for the construction of green cities. Only with continuous improvement at the technological level do green industries have the driving force for sustainable development, and traditional high-energy consuming and high-pollution industries can be continuously eliminated so as to promote the construction of green cities. (3) Based on the correlation analysis between each indicator and comprehensive scores, this study also pointed out that there is a significant gap in the level of green city construction among different regions in China. Full article

Novel goose parvovirus (NGPV), a genetic variant of goose parvovirus, has been spreading throughout China since 2015 and mainly infects ducklings with the symptoms of growth retardation, beak atrophy, and protruding tongue, leading to huge economic losses every year. A safe and effective vaccine is urgently needed to control NGPV infection. In this study, virus-like particles (VLPs) of NPGV were assembled and evaluated for their immunogenicity. The VP2 protein of NGPV was expressed in Spodoptera frugiperda insect cells using baculovirus as vector. The VP2 protein was efficiently expressed in the nucleus of insect cells, and the particles with a circular or hexagonal shape and a diameter of approximately 30 nm, similar to the NGPV virion, were observed using transmission electron microscopy (TEM). The purified particles were confirmed to be composed of VP2 using western blot and TEM, indicating that the VLPs of NGPV were successfully assembled. Furthermore, the immunogenicity of the VLPs of NGPV was evaluated in Cherry Valley ducks. The level of NGPV serum antibodies increased significantly at 1–4 weeks post-immunization. No clinical symptoms or deaths of ducks occurred in all groups after being challenged with NGPV at 4 weeks post-immunization. There was no viral shedding in the immunized group. However, viral shedding was detected at 3–7 days post-challenge in the non-immunized group. Moreover, VLPs can protect ducks from histopathological lesions caused by NGPV and significantly reduce viral load in tissue at 5 days post-challenge. Based on these findings, NGPV VLPs are promising candidates for vaccines against NGPV. Full article

In recent decades, natural disasters have increased drastically, with slope movements being the most damaging geological hazard, causing thousands of deaths and considerable economic losses. To reduce these losses, it is necessary to carry out cartographies that spatially delimit these risks, preventing and mitigating the effects through the analysis of susceptibility in areas of great environmental value, as is the case of the Arribes del Duero Natural Park. For this purpose, different statistical methods combined with Geographic Information Systems have been developed. The susceptibility assessment methodology is carried out by integrating different thematic layers: lithology, geomorphology (slopes, curvature, aspect), hydrogeology and vegetation, performing map algebra and taking into consideration their weighting using deterministic methods (analytical hierarchy method). The susceptibility results are grouped into Very High, High, Medium, Low and Very Low so that the areas of Very High susceptibility correspond to areas of the high slope, without vegetation, south facing, with a lithology of quartzites, metapelites, and gneisses (canyons, steep valleys) and, in the case of very low susceptibility, with a lithology of quartzites, metapelites, and gneisses, On the contrary, the sectors of lower susceptibility coincide with flat areas, denser vegetation, north facing, with a lithology of conglomerates, pebbles, sands and clays, such as erosion surfaces or valley bottoms. The analysis carried out in this current investigation will allow the territorial delimitation of problem areas and the establishment of risk mitigation and management measures. Full article

The Valley of Death confronts industrial biotechnology with a significant challenge to the commercialization of products. Fortunately, with the integration of computation, automation and artificial intelligence (AI) technology, the industrial biotechnology accelerates to cross the Valley of Death. The Fourth Industrial Revolution (Industry 4.0) has spurred advanced development of intelligent biomanufacturing, which has evolved the industrial structures in line with the worldwide trend. To achieve this, intelligent biomanufacturing can be structured into three main parts that comprise digitalization, modeling and intellectualization, with modeling forming a crucial link between the other two components. This paper provides an overview of mechanistic models, data-driven models and their applications in bioprocess development. We provide a detailed elaboration of the hybrid model and its applications in bioprocess engineering, including strain design, process control and optimization, as well as bioreactor scale-up. Finally, the challenges and opportunities of biomanufacturing towards Industry 4.0 are also discussed. Full article

P2Et is the standardized extract of Caesalpinia spinosa (C. spinosa), which has shown the ability to reduce primary tumors and metastasis in animal models of cancer, by mechanisms involving the increase in intracellular Ca++, reticulum stress, induction of autophagy, and subsequent activation of the immune system. Although P2Et has been shown to be safe in healthy individuals, the biological activity and bioavailability can be increased by improving the dosage form. This study investigates the potential of a casein nanoparticle for oral administration of P2Et and its impact on treatment efficacy in a mouse model of breast cancer with orthotopically transplanted 4T1 cells. Animals were treated with either free or encapsulated oral P2Et orally or i.p. Tumor growth and macrometastases were evaluated. All P2Et treatments significantly delayed tumor growth. The frequency of macrometastasis was reduced by 1.1 times with P2Et i.p., while oral P2Et reduced it by 3.2 times and nanoencapsulation reduced it by 3.57 times. This suggests that nanoencapsulation led to higher doses of effective P2Et being delivered, slightly improving bioavailability and biological activity. Therefore, the results of this study provide evidence to consider P2Et as a potential adjuvant in the treatment of cancer, while the nanoencapsulation of P2Et provides a novel perspective on the delivery of these functional ingredients. Full article