Complete Blood Count and Biochemistry Reference Intervals for Healthy Adult Donkeys in the United States
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Approval
2.2. Animals and Sample Collection
2.3. Laboratory Analyses
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Measurand | Method |
---|---|
Albumin | Bromocresol green dye-binding |
AST | NADH oxidation |
Electrolytes | Indirect ion-selective potentiometry |
Bicarbonate | Phosphoenolpyruvate carboxylase based |
Bilirubin, total | Jendrassik-Grof based- Diazo method (diazonium ion) |
Bilirubin, direct | Jendrassik-Grof based- (diazotized sulfanilic acid) |
Calcium | 5-nitro-5′-methyl-BAPTA (NM-BAPTA) |
Cholesterol | Cholesterol esterase |
Creatinine kinase | Creatine phosphate cleavage |
Creatinine | Modified Jaffe |
GGT | L-gamma-glutamyl-3-carboxy-4- nitroanilde substrate |
Glucose | Hexokinase |
GLDH | Alpha-oxoglutarate substrate |
Magnesium | Xylidyl blue, diazonium salt |
Phosphate | Ammonium molybdate |
SDH | D-fructose substrate |
Triglycerides | Lipoprotein lipase |
Total protein | Biuret |
Urea Nitrogen | Urease- kinetic |
Measurand | n | Reason(s) |
---|---|---|
Manual WBC Differential | 17 | Uneven distribution of WBCs on smears causing erroneous differential percentages/counts |
Platelet | 21 | Marked platelet clumping causing falsely decreased counts |
MPV | 21 | Marked platelet clumping causing falsely increased MPV |
TP (on CBC) | 1 | Moderate to marked hemolysis causing interference with refractometer |
Electrolytes | 1 | Multiple outliers from single sample, suggesting subclinical disease |
Calcium | 1 | Low outlier associated with low albumin, presumed artifact of decreased protein binding |
Glucose | 12 | Very low values (<50 mg/dL) incompatible with health, presumed artifact of delayed serum separation from cells (10 of 12 outliers were received as whole clotted blood). |
AST, SDH, GLDH | 1 | Multiple high hepatocellular enzyme outliers from a single sample, suggesting subclinical liver disease |
GGT | 6 | Multiple high outliers associated with single herd. Samples received as whole clotted blood, suspect pre-analytical artifact |
GGT | 3 | Multiple high outliers associated with single herd. Follow-up communication with submitter reveals history of liver flukes in herds. |
Cholesterol and Triglycerides | 3 | Samples with both lipid values marked as suspect or outlier data (high), suggesting subclinical disease |
Triglycerides | 1 | Single sample with marked hypertriglyceridemia, incompatible with health |
CK | 5 | Samples with CK > 800, suggestive of traumatic handling or restraint |
Measurand | Units | n | Mean | SD | Median | Min | Max | p-Value * | Dist | Method | LRL of RI | URL of RI | CI of LRL | CI of URL |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Hematocrit | L/L | 109 | 39.1 | 6.5 | 39.0 | 26 | 54 | 0.184 | G | P | 28 | 53 | 27–29 | 51–56 |
RBC | 1012/L | 109 | 6.4 | 1.2 | 6.2 | 4.2 | 8.9 | 0.023 | NG | NP | 4.4 | 8.8 | 4.2–4.7 | 8.4–8.9 |
Hemoglobin | g/L | 109 | 131 | 21 | 130 | 89 | 177 | 0.223 | G | P | 93 | 175 | 89–101 | 170–177 |
MCV | fL | 109 | 61 | 3.9 | 61 | 51 | 70 | 0.162 | G | P | 53 | 68 | 51–54 | 67–69 |
MCHC | g/L | 109 | 335 | 7 | 330 | 310 | 350 | <0.001 | NG | NP | 320 | 350 | 310–320 | 340–350 |
MCH | pg | 109 | 21 | 1.4 | 21 | 17 | 23 | <0.001 | NG | NP | 18 | 23 | 17–18 | 22–23 |
RDW | % | 109 | 17.3 | 0.9 | 17.1 | 15.9 | 21.6 | 0.753 | G | P | 16.1 | 19.4 | 16.0–16.2 | 18.9–19.9 |
WBC | 109/L | 109 | 10.0 | 2.6 | 9.7 | 5.3 | 18.4 | 0.996 | G | P | 5.8 | 16.0 | 5.5–6.3 | 14.9–17.2 |
Neutrophil | 109/L | 92 | 4.7 | 1.6 | 4.4 | 2.2 | 10.6 | 0.390 | G | P | 2.2 | 8.5 | 2.0–2.4 | 7.8–9.3 |
Lymphocyte | 109/L | 92 | 4.7 | 2.2 | 4.4 | 1.1 | 11.1 | 0.732 | G | P | 1.5 | 9.9 | 1.3–1.7 | 8.9–10.8 |
Monocyte | 109/L | 92 | 0.4 | 0.2 | 0.3 | 0 | 1.2 | 0.001 | NG | NP | 0 | 1.0 | 0–0.1 | 0.8–1.2 |
Eosinophil | 109/L | 92 | 0.4 | 0.4 | 0.3 | 0 | 1.9 | 0.001 | NG | NP | 0 | 1.7 | 0–0 | 1.4–1.9 |
Basophil | 109/L | 92 | 0 | 0.1 | 0 | 0 | 0.3 | <0.001 | NG | NP | 0 | 0.1 | 0–0 | 0.1–0.3 |
Platelet | 109/L | 61 | 197 | 85 | 195 | 81 | 396 | 0.003 | NG | NP | 84 | 380 | 81–89 | 322–396 |
MPV | fL | 61 | 6.1 | 0.8 | 6.1 | 4.8 | 8.7 | 0.742 | G | P | 4.8 | 8.1 | 4.7–5.0 | 7.7–8.6 |
TP | g/L | 108 | 71 | 5 | 70 | 56 | 84 | 0.402 | G | P | 60 | 81 | 59–62 | 79–82 |
Measurand | Units | n | Mean | SD | Median | Min | Max | p-Value * | Dist | Method | LRL of RI | URL of RI | CI of LRL | CI of URL |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sodium | mmol/L | 116 | 134 | 3.2 | 135 | 127 | 144 | 0.047 | NG | NP | 127 | 140 | 127–128 | 139–144 |
Potassium | mmol/L | 116 | 4.6 | 0.6 | 4.5 | 3.1 | 5.8 | 0.545 | G | P | 3.4 | 5.7 | 3.3–3.6 | 5.6–5.8 |
Chloride | mmol/L | 116 | 100 | 3.0 | 100 | 93 | 107 | 0.058 | G | P | 94 | 106 | 93–95 | 105–107 |
Bicarbonate | mmol/L | 116 | 23 | 2.4 | 23 | 15 | 29 | 0.002 | NG | NP | 16 | 27 | 15–19 | 26–29 |
Anion Gap | mmol/L | 116 | 17 | 3.2 | 16 | 11 | 28 | 0.020 | NG | NP | 12 | 25 | 11–12 | 25–28 |
Na/K Ratio | 116 | 30 | 4.1 | 30 | 22 | 45 | 0.089 | G | P | 23 | 40 | 23–24 | 38–41 | |
Urea nitrogen | mmol/L | 117 | 4.3 | 1.5 | 4.3 | 0.7 | 9.3 | 0.001 | NG | NP | 1.1 | 7.5 | 0.7–1.8 | 6.4–9.3 |
Creatinine | umol/L | 117 | 88 | 18 | 88 | 44 | 133 | <0.001 | NG | NP | 53 | 133 | 44–53 | 115–133 |
Calcium | mmol/L | 116 | 3.0 | 0.1 | 3.0 | 2.6 | 3.2 | 0.128 | G | P | 2.7 | 3.2 | 2.7–2.8 | 3.1–3.2 |
Phosphorus | mmol/L | 117 | 1.2 | 0.2 | 1.2 | 0.7 | 1.8 | 0.741 | G | P | 0.7 | 1.6 | 0.7–0.8 | 1.6–1.7 |
Magnesium | mmol/L | 117 | 0.7 | 0.1 | 0.7 | 0.5 | 1.2 | 0.016 | NG | NP | 0.5 | 1.0 | 0.5–0.6 | 1.0–1.2 |
Total protein | g/L | 117 | 67 | 5 | 67 | 48 | 81 | 0.081 | G | P | 57 | 78 | 56–58 | 76–79 |
Albumin | g/L | 117 | 30 | 4.0 | 30 | 17 | 37 | 0.015 | NG | NP | 20 | 36 | 17–23 | 35–37 |
Globulin | g/L | 117 | 37 | 5 | 37 | 28 | 51 | 0.226 | G | P | 29 | 48 | 28–30 | 46–49 |
A/G Ratio | 117 | 0.8 | 0.2 | 0.8 | 0.4 | 1.2 | <0.001 | NG | NP | 0.5 | 1.1 | 0.4–0.5 | 1.1–1.2 | |
Glucose | mmol/L | 105 | 4.8 | 0.8 | 4.8 | 2.4 | 7.4 | 0.301 | G | P | 3.2 | 6.5 | 3.0–3.4 | 6.2–6.8 |
AST | U/L | 116 | 367 | 84 | 364 | 201 | 667 | 0.744 | G | P | 223 | 555 | 208–238 | 525–587 |
SDH | U/L | 116 | 4 | 3.2 | 4 | 2 | 29 | <0.001 | NG | NP | 2 | 10 | 2–2 | 9–29 |
GLDH | U/L | 116 | 4 | 2.2 | 3 | 0 | 14 | <0.001 | NG | NP | 0 | 9 | 0–1 | 8–14 |
GGT | U/L | 107 | 28 | 12 | 24 | 7 | 77 | 0.200 | G | P | 10 | 55 | 9–12 | 52–65 |
Bilirubin | umol/L | 117 | 1.7 | 0.9 | 1.7 | 0 | 3.4 | <0.001 | NG | NP | 0 | 3.4 | 0–0 | 1.7–3.4 |
Dir Bilirubin | umol/L | 117 | 0 | 0.3 | 0 | 0 | 1.7 | <0.001 | NG | NP | 0 | 1.7 | 0–0 | 0–1.7 |
Indir Bilirubin | umol/L | 117 | 1.7 | 0.9 | 1.7 | 0 | 1.7 | <0.001 | NG | NP | 0 | 1.7 | 0–0 | 1.7–1.7 |
CK | U/L | 112 | 231 | 105 | 210 | 47 | 725 | 0.055 | G | P | 95 | 500 | 86–105 | 446–569 |
Cholesterol | mmol/L | 114 | 2.0 | 0.4 | 2.0 | 1.3 | 3.2 | 0.281 | G | P | 1.4 | 2.4 | 1.3–1.5 | 2.8–3.1 |
Triglycerides | mmol/L | 113 | 1.2 | 0.5 | 1.2 | 0.1 | 2.4 | 0.273 | G | NP | 0.3 | 2.2 | 0.2–0.4 | 2.0–2.3 |
TG (mmol/L) | GGT (U/L) | |
---|---|---|
AHDC Donkeys (current study) | 0.3–2.2 | 10–55 |
AHDC Equines * | 0.2–0.7 | 8–33 |
Previous US Donkeys (Zinkl et al. 1990) [14] | NA | 10–128 |
Previous UK Donkeys (French & Patrick 1995) [20] | 0.2–4.3 | 8–49 |
Texas Donkeys (Dugat et al. 2010) [12] | 0.3–1.4 | NA |
UK Donkeys (Burden et al. 2016) [13] | 0.6–2.8 | 14–69 |
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Goodrich, E.L.; Webb, J.L. Complete Blood Count and Biochemistry Reference Intervals for Healthy Adult Donkeys in the United States. Animals 2024, 14, 2018. https://doi.org/10.3390/ani14142018
Goodrich EL, Webb JL. Complete Blood Count and Biochemistry Reference Intervals for Healthy Adult Donkeys in the United States. Animals. 2024; 14(14):2018. https://doi.org/10.3390/ani14142018
Chicago/Turabian StyleGoodrich, Erin L., and Julie L. Webb. 2024. "Complete Blood Count and Biochemistry Reference Intervals for Healthy Adult Donkeys in the United States" Animals 14, no. 14: 2018. https://doi.org/10.3390/ani14142018
APA StyleGoodrich, E. L., & Webb, J. L. (2024). Complete Blood Count and Biochemistry Reference Intervals for Healthy Adult Donkeys in the United States. Animals, 14(14), 2018. https://doi.org/10.3390/ani14142018