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Toxins 2018, 10(2), 85; https://doi.org/10.3390/toxins10020085

Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study

1
School of Medicine, Universidad Cooperativa de Colombia, Sede Medellín, Street 50 A N° 41-20, Medellín 050010, Colombia
2
Malaria Group, School of Medicine, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia
3
Ophidism/Scorpionism Program, Food and Pharmaceutical Sciences Faculty, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia
4
Reproduction Group, School of Medicine, University of Antioquia UdeA, Street 70 N° 52-21, Medellín 050010, Colombia
*
Author to whom correspondence should be addressed.
Received: 29 November 2017 / Revised: 6 February 2018 / Accepted: 13 February 2018 / Published: 17 February 2018
(This article belongs to the Section Animal Venoms)
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Abstract

The Colombian rattlesnake Crotalus durissus cumanensis is distributed in three geographic zones of the country: the Atlantic Coast, the upper valley of the Magdalena River, and the eastern plains of the Colombian Orinoquía. Its venom induces neurological symptoms, such as eyelid ptosis, myasthenic facies, and paralysis of the respiratory muscles, which can lead to death. Identification and analysis of C. d. cumanensis showed nine groups of proteins responsible for the neurotoxic effect, of which the crotoxin complex was the most abundant (64.71%). Immunorecognition tests of C. d. cumanensis showed that the use of a commercial antivenom manufactured in Mexico resulted in immunoreactivity. View Full-Text
Keywords: C. d. cumanensis venom; antivenom; HPLC-nESI-MS/MS; peptide identification; Key Contribution; This work carried out a proteomic analysis of C. d. cumanensis from Colombia by using HPLC-nESI MS/MS and subsequently was evaluated the effect of a commercial antivenom against the venom C. d. cumanensis venom; antivenom; HPLC-nESI-MS/MS; peptide identification; Key Contribution; This work carried out a proteomic analysis of C. d. cumanensis from Colombia by using HPLC-nESI MS/MS and subsequently was evaluated the effect of a commercial antivenom against the venom
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Quintana-Castillo, J.C.; Vargas, L.J.; Segura, C.; Estrada-Gómez, S.; Bueno-Sánchez, J.C.; Alarcón, J.C. Characterization of the Venom of C. d. cumanesis of Colombia: Proteomic Analysis and Antivenomic Study. Toxins 2018, 10, 85.

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