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77 Results Found

  • Article
  • Open Access
14 Citations
3,365 Views
13 Pages

The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH

  • Tianheng Ren,
  • Maojie He,
  • Zixin Sun,
  • Feiquan Tan,
  • Peigao Luo,
  • Zongxiang Tang,
  • Shulan Fu,
  • Benju Yan,
  • Zhenglong Ren and
  • Zhi Li

21 March 2019

Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome. In this study, five different oligonucleotide probes were used with ND-FISH to examine 21...

  • Article
  • Open Access
9 Citations
2,280 Views
14 Pages

14 September 2023

As a perennial herb in Triticeae, Elymus dahuricus is widely distributed in Qinghai–Tibetan Plateau and Central Asia. It has been used as high-quality fodders for improving degraded grassland. The genomic constitution of E. dahuricus (2n = 6x =...

  • Article
  • Open Access
9 Citations
2,774 Views
14 Pages

13 August 2022

Thinopyrum intermedium possesses many desirable agronomic traits that make it a valuable genetic source for wheat improvement. The precise identification of individual chromosomes of allohexaploid Th. intermedium is a challenge due to its three sub-g...

  • Article
  • Open Access
1 Citations
1,801 Views
13 Pages

ND-FISH with New Oligo Probes for Chromosome Identification of Cichorium intybus Revealing Karyotypic Variation and Divergence of Asteraceae Species

  • Meiling Chen,
  • Chengzhi Jiang,
  • Doudou Huang,
  • Zhiqiang Zheng,
  • Wenzhuo Yang,
  • Guangrong Li,
  • Chun Fu,
  • Hong Liao,
  • Wencong Long and
  • Yaojun Yang
  • + 1 author

7 November 2024

Chicory (Cichorium intybus L., 2n = 18), belonging to the Asteraceae family, exhibits significant edible, medicinal, and pasture values. Moderate research has been performed on identifying Chicory species’ chromosomes using fluorescence in situ...

  • Communication
  • Open Access
45 Citations
8,234 Views
10 Pages

5 December 2016

Oligonucleotide probes that can be used for non-denaturing fluorescence in situ hybridization (ND-FISH) analysis are convenient tools for identifying chromosomes of wheat (Triticum aestivum L.) and its relatives. New oligonucleotide probes, Oligo-HvT...

  • Article
  • Open Access
45 Citations
6,440 Views
11 Pages

Oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (ND-FISH) technique are widely used to analyze plant chromosomes because they are convenient tools. New oligonucleotide probes, Oligo-Ku, Oligo-3B117.1, Oligo-3B117.2, O...

  • Brief Report
  • Open Access
39 Citations
4,861 Views
8 Pages

New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds

  • Wei Xi,
  • Zongxiang Tang,
  • Shuyao Tang,
  • Zujun Yang,
  • Jie Luo and
  • Shulan Fu

Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify ali...

  • Article
  • Open Access
8 Citations
2,396 Views
14 Pages

21 December 2022

Thinopyrum intermedium (2n = 6x = 42, JJJSJSStSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat-Th. interm...

  • Article
  • Open Access
7 Citations
2,665 Views
19 Pages

25 May 2022

Germplasm is the material basis for crop genetic improvement and related basic research. Knowledge of genetic diversity present in wheat is the prerequisite for wheat breeding and improvement. Non-denaturing fluorescence in situ hybridization (ND-FIS...

  • Article
  • Open Access
3 Citations
2,233 Views
10 Pages

14 September 2022

Wheat (Triticum aestivum L.) is rich in tandem repeats, and this is helpful in studying its karyotypic evolution. Some tandem repeats have not been assembled into the wheat genome sequence. Alignment using the blastn tool in the B2DSC web server indi...

  • Article
  • Open Access
1 Citations
1,233 Views
15 Pages

Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from Thinopyrum intermedium in Wheat

  • Chengzhi Jiang,
  • Yujie Luo,
  • Doudou Huang,
  • Meiling Chen,
  • Ennian Yang,
  • Guangrong Li and
  • Zujun Yang

20 May 2025

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and r...

  • Article
  • Open Access
5 Citations
2,434 Views
15 Pages

10 September 2022

In this study, a novel T6RS.6AL translocation line, 117-6, was selected from a cross between common Chuannong25 (CN25) wheat and Qinling rye. The results of nondenaturing fluorescence in situ hybridization (ND-FISH) and PCR showed that 117-6 containe...

  • Article
  • Open Access
13 Citations
5,503 Views
10 Pages

Identification of Novel Chromosomal Aberrations Induced by 60Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines

  • Jie Zhang,
  • Yun Jiang,
  • Yuanlin Guo,
  • Guangrong Li,
  • Zujun Yang,
  • Delin Xu and
  • Pu Xuan

14 December 2015

Mutations induced by radiation are widely used for developing new varieties of plants. To better understand the frequency and pattern of irradiation-induced chromosomal rearrangements, we irradiated the dry seeds of Chinese Spring (CS)-Dasypyrum vill...

  • Article
  • Open Access
6 Citations
2,606 Views
16 Pages

Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

  • Jie Zhang,
  • Shuyao Tang,
  • Tao Lang,
  • Ying Wang,
  • Hai Long,
  • Guangbing Deng,
  • Qian Chen,
  • Yuanlin Guo,
  • Pu Xuan and
  • Yun Jiang
  • + 1 author

18 May 2022

The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–D. villosum 3V#3 (3D) substitution line...

  • Article
  • Open Access
1 Citations
1,612 Views
15 Pages

22 August 2024

The wild species Thinopyrum intermedium (genome JJJSJSStSt) serves as a valuable germplasm resource providing novel diseases resistance and agronomically important genes for wheat improvement. Two wheat-Th. intermedium partial amphiploids, TAI7045 (2...

  • Article
  • Open Access
2 Citations
2,005 Views
14 Pages

25 April 2024

Thinopyrum intermedium (2n = 6x = 42, EeEeEbEbStSt or JJJsJsStSt) contains a large number of genes that are highly adaptable to the environment and immune to a variety of wheat diseases, such as powdery mildew, rust, and yellow dwarf, making it an im...

  • Article
  • Open Access
11 Citations
4,542 Views
16 Pages

29 January 2019

A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a c...

  • Article
  • Open Access
6 Citations
2,668 Views
16 Pages

17 November 2023

An elite hexaploid triticale Yukuri from Australia was used as a bridge for transferring valuable genes from Secale cereale L. into common wheat for enriching the genetic variability of cultivated wheat. Non-denaturing-fluorescence in situ hybridizat...

  • Article
  • Open Access
38 Citations
5,354 Views
15 Pages

3 January 2019

The wild species, Thinopyrum intermedium. (Genome StStJSJSJJ), serves as a valuable germplasm resource providing novel genes for wheat improvement. In the current study, non-denaturing fluorescence in situ hybridization (ND-FISH) with multiple probes...

  • Article
  • Open Access
3 Citations
2,322 Views
15 Pages

13 July 2022

The construction of the 28-chromosome karyotype of Dasypyrum breviaristatum was undertaken using multicolor non-denaturing fluorescent in situ hybridization (ND-FISH) and Oligo-FISH painting protocols. A novel wheat-D. breviaristatum line D2138 conta...

  • Article
  • Open Access
22 Citations
4,071 Views
14 Pages

14 June 2019

To determine the composition of chromosome aberrations in a wheat‒Dasypyrum breviaristatum substitution line with seeds treated by a dose of gamma-rays (200 Gy), sequential non-denaturing fluorescence in situ hybridization (ND-FISH) with multiple oli...

  • Article
  • Open Access
22 Citations
4,182 Views
16 Pages

4 December 2021

Rare earth elements and yttrium (REY) are widely recognized as strategic materials for advanced technological applications. Deep-sea sediments from the eastern South Pacific and central North Pacific were first reported as potential resources contain...

  • Article
  • Open Access
1,260 Views
22 Pages

Rapid Detection of Epinephelus Species Substitution in the Greek Market Using High-Resolution Melting Analysis

  • Evanthia Chatzoglou,
  • Nefeli Tsaousi,
  • Ariadni Spetsieri,
  • Emmanouil E. Malandrakis and
  • Helen Miliou

22 February 2025

Background/Objectives: Fish are vital in the Mediterranean diet, offering protein, nutrients, and ω-3 fatty acids. Greek consumers favor wild-caught, high-value fish like the dusky grouper (Epinephelus marginatus) classified as “vulnerabl...

  • Article
  • Open Access
885 Views
18 Pages

Impact of Alien Chromosome Introgression from Thinopyrum ponticum on Wheat Grain Traits

  • Shuwei Zhang,
  • Yu Zhang,
  • Ting Hu,
  • Linying Li,
  • Zihao Wang,
  • Linyi Qiao,
  • Lifang Chang,
  • Xin Li,
  • Zhijian Chang and
  • Xiaojun Zhang
  • + 1 author

4 October 2025

Structural variation (SV) serves as a fundamental driver of phenotypic diversity and environmental adaptation in plants and animals, significantly influencing key agronomic traits in crops. Common wheat (Triticum aestivum L.), an allohexaploid specie...

  • Article
  • Open Access
26 Citations
7,379 Views
16 Pages

Memory Function in Feeding Habit Transformation of Mandarin Fish (Siniperca chuatsi)

  • Yaqi Dou,
  • Shan He,
  • Xu-Fang Liang,
  • Wenjing Cai,
  • Jie Wang,
  • Linjie Shi and
  • Jiao Li

Mandarin fish refuse dead prey fish or artificial diets and can be trained to transform their inborn feeding habit. To investigate the effect of memory on feeding habit transformation, we compared the reaction time to dead prey fish and the success r...

  • Article
  • Open Access
18 Citations
4,681 Views
18 Pages

Electrospinning Fabrication and Cytocompatibility Investigation of Nanodiamond Particles-Gelatin Fibrous Tubular Scaffolds for Nerve Regeneration

  • Elena Olăreț,
  • Diana-Maria Drăgușin,
  • Andrada Serafim,
  • Adriana Lungu,
  • Aida Șelaru,
  • Alexandra Dobranici,
  • Sorina Dinescu,
  • Marieta Costache,
  • Iulian Boerașu and
  • Izabela-Cristina Stancu
  • + 3 authors

27 January 2021

This paper reports the electrospinning fabrication of flexible nanostructured tubular scaffolds, based on fish gelatin (FG) and nanodiamond nanoparticles (NDs), and their cytocompatibility with murine neural stem cells. The effects of both nanofiller...

  • Article
  • Open Access
6 Citations
3,463 Views
20 Pages

29 April 2022

The interspecific recombination of the mitochondrial (mt) genome, if not an experimental artifact, may result from interbreeding of species with broken reproductive barriers, which, in turn, is a frequent consequence of human activities including spe...

  • Article
  • Open Access
7 Citations
2,871 Views
20 Pages

10 November 2023

Ectothermic fish exposure to hypothermal stress requires adjusting their metabolic molecular machinery, which was investigated using Indian medaka (Oryzias dancena; 10 weeks old, 2.5 ± 0.5 cm) cultured in fresh water (FW) and seawater (SW; 35&...

  • Article
  • Open Access
10 Citations
3,193 Views
20 Pages

Time- and Dose-Dependent Effects of Dietary Deoxynivalenol (DON) in Rainbow Trout (Oncorhynchus mykiss) at Organism and Tissue Level

  • Paraskevi Koletsi,
  • Geert F. Wiegertjes,
  • Elisabeth A. M. Graat,
  • Philip Lyons and
  • Johan Schrama

20 November 2022

This study with juvenile rainbow trout evaluated the effects of dietary exposure to deoxynivalenol (DON) at industrially relevant doses (up to 1.6 mg/kg) on growth performance, the liver, and the gastrointestinal tract. Fifteen groups of 30 fish each...

  • Brief Report
  • Open Access
2,033 Views
9 Pages

Population Structure of Aetobatus narinari (Myliobatiformes) Caught by the Artisanal Fishery in Northeast Brazil

  • Priscila R. V. Araújo,
  • Bautisse D. Postaire,
  • Kevin A. Feldheim,
  • Kim Bassos-Hull,
  • Rosangela Lessa and
  • Matthew J. Ajemian

28 June 2024

In Brazil, the Whitespotted Eagle Ray Aetobatus narinari is considered data-deficient due to the scarcity of basic information on its biology, ecology, and vulnerability to capture. Despite this, the species is caught by artisanal fishing along most...

  • Article
  • Open Access
3,129 Views
18 Pages

Quantification of Acetaminophen, Ibuprofen, and β-Blockers in Wastewater and River Water Bodies During the COVID-19 Pandemic

  • Neliswa Mpayipheli,
  • Anele Mpupa,
  • Ntakadzeni Edwin Madala and
  • Philiswa Nosizo Nomngongo

The consumption of pharmaceuticals during the COVID-19 pandemic increased significantly. As such, over-the-counter drugs such as acetaminophen (ACT), ibuprofen (IBU), metoprolol (MET), and propranolol (PRO) were among the pharmaceuticals that were wi...

  • Feature Paper
  • Article
  • Open Access
34 Citations
4,192 Views
18 Pages

Fabrication and Biocompatibility Evaluation of Nanodiamonds-Gelatin Electrospun Materials Designed for Prospective Tissue Regeneration Applications

  • Aida Şelaru,
  • Diana-Maria Drăgușin,
  • Elena Olăreț,
  • Andrada Serafim,
  • Doris Steinmüller-Nethl,
  • Eugeniu Vasile,
  • Horia Iovu,
  • Izabela-Cristina Stancu,
  • Marieta Costache and
  • Sorina Dinescu

11 September 2019

Due to the reduced ability of most harmed tissues to self-regenerate, new strategies are being developed in order to promote self-repair assisted or not by biomaterials, among these tissue engineering (TE). Human adipose-derived mesenchymal stem cell...

  • Article
  • Open Access
2 Citations
3,190 Views
13 Pages

Recombinant Domain of Flagellin Promotes In Vitro a Chemotactic Inflammatory Profile in Human Immune Cells Independently of a Dendritic Cell Phenotype

  • Roxana González-Stegmaier,
  • Adam Aguirre,
  • Constanza Cárcamo,
  • Patricia Aguila-Torres and
  • Franz Villarroel-Espíndola

Flagellin is the major component of the flagellum in gram-positive and -negative bacteria and is also the ligand for the Toll-like receptor 5 (TLR5). The activation of TLR5 promotes the expression of proinflammatory cytokines and chemokines and the s...

  • Article
  • Open Access
1,195 Views
16 Pages

A Novel Clonorchis sinensis Mitogenome: Elucidating Multiregional Strain Phylogeny and Revising the Digenean Mitochondrial Genome Tree

  • Yuxuan Liu,
  • Kaisong Hu,
  • Yanan Zhang,
  • Zhili Chen,
  • Haoyu Zheng,
  • Yuexi Teng,
  • Fang Wang and
  • Jingtong Zheng

28 August 2025

Clonorchis sinensis, a parasitic liver fluke, is the primary aetiological agent of clonorchiasis, a disease predominantly characterized by liver-related clinical manifestations. Currently, research on the complete mitochondrial (mt) genome of local C...

  • Communication
  • Open Access
6 Citations
4,629 Views
13 Pages

4 December 2022

The use of antibiotics in ornamental fish is not regulated, as they are not intended for human consumption. Although antibiotic resistant bacteria have been detected in ornamental fish worldwide, there have been no studies to look at the situation in...

  • Article
  • Open Access
4 Citations
2,044 Views
7 Pages

28 September 2021

Alien fish introductions, conducted towards the ichthyofauna enhancement in local drainages, have been occasionally proved harmful for the indigenous freshwater fish populations. The present study was designed to assess the impact of stocking activit...

  • Article
  • Open Access
2 Citations
2,495 Views
14 Pages

19 July 2023

This study aimed to investigate the immunoprotective effect of Coptis chinensis (CC) on Streptococcus agalactiae (SA) infection in tilapia. Experimental fish were randomly divided into two groups feeding on a normal diet (ND) and a CC-supplemented di...

  • Article
  • Open Access
10 Citations
5,339 Views
12 Pages

4 November 2021

Seafood, especially the traditional one in Taiwan, is rarely sourced from a fixed species and routinely from similar species depending on their availability. Hence, the species composition of seafood can be complicated. While a DNA-based approach has...

  • Article
  • Open Access
9 Citations
2,606 Views
17 Pages

Amblyopinae Mitogenomes Provide Novel Insights into the Paraphyletic Origin of Their Adaptation to Mudflat Habitats

  • Zhenming Lü,
  • Yantao Liu,
  • Shijie Zhao,
  • Jiaqi Fang,
  • Kehua Zhu,
  • Jing Liu,
  • Li Gong,
  • Liqin Liu and
  • Bingjian Liu

22 February 2023

The water-to-land transition is one of the most important events in evolutionary history of vertebrates. However, the genetic basis underlying many of the adaptations during this transition remains unclear. Mud-dwelling gobies in the subfamily Amblyo...

  • Article
  • Open Access
11 Citations
6,209 Views
31 Pages

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

  • Gilbert Audira,
  • Petrus Siregar,
  • Kelvin H.-C. Chen,
  • Marri Jmelou M. Roldan,
  • Jong-Chin Huang,
  • Hong-Thih Lai and
  • Chung-Der Hsiao

Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka compared to zebrafish, especially with regard to its behavior. Thus,...

  • Article
  • Open Access
56 Citations
15,183 Views
15 Pages

Seafood Consumption, Omega-3 Fatty Acids Intake, and Life-Time Prevalence of Depression in the PREDIMED-Plus Trial

  • Almudena Sánchez-Villegas,
  • Jacqueline Álvarez-Pérez,
  • Estefanía Toledo,
  • Jordi Salas-Salvadó,
  • Carolina Ortega-Azorín,
  • Maria Dolores Zomeño,
  • Jesús Vioque,
  • Jose Alfredo Martínez,
  • Dora Romaguera and
  • Lluis Serra-Majem
  • + 28 authors

18 December 2018

Background: The aim of this analysis was to ascertain the type of relationship between fish and seafood consumption, omega-3 polyunsaturated fatty acids (ω-3 PUFA) intake, and depression prevalence. Methods: Cross-sectional analyses of the PRED...

  • Article
  • Open Access
8 Citations
2,826 Views
20 Pages

Functional Additives in a Selected European Sea Bass (Dicentrarchus labrax) Genotype: Effects on the Stress Response and Gill Antioxidant Response to Hydrogen Peroxide (H2O2) Treatment

  • Antonio Serradell,
  • Daniel Montero,
  • Genciana Terova,
  • Simona Rimoldi,
  • Alex Makol,
  • Félix Acosta,
  • Aline Bajek,
  • Pierrick Haffray,
  • François Allal and
  • Silvia Torrecillas

11 July 2023

Functional ingredients have profiled as suitable candidates for reinforcing the fish antioxidant response and stress tolerance. In addition, selective breeding strategies have also demonstrated a correlation between fish growth performance and suscep...

  • Article
  • Open Access
1 Citations
1,599 Views
16 Pages

Mitochondrial Genomes of Distant Fish Hybrids Reveal Maternal Inheritance Patterns and Phylogenetic Relationships

  • Shixi Chen,
  • Fardous Mohammad Safiul Azam,
  • Li Ao,
  • Chanchun Lin,
  • Jiahao Wang,
  • Rui Li and
  • Yuanchao Zou

24 July 2025

As distant hybridization has profound implications for evolutionary biology, aquaculture, and biodiversity conservation, this study aims to elucidate patterns of maternal inheritance, genetic divergence, and phylogenetic relationships by synthesizing...

  • Article
  • Open Access
1 Citations
2,998 Views
14 Pages

20 January 2023

The family Ophichthidae has the largest number and the most various species (about 359 valid species) in the order Anguilliformes worldwide. Both morphological and molecular characteristics have been used to assess their taxonomic status. However, du...

  • Article
  • Open Access
8 Citations
3,297 Views
20 Pages

Gill Oxidative Stress Protection through the Use of Phytogenics and Galactomannan Oligosaccharides as Functional Additives in Practical Diets for European Sea Bass (Dicentrarchus labrax) Juveniles

  • Antonio Serradell,
  • Daniel Montero,
  • Álvaro Fernández-Montero,
  • Genciana Terova,
  • Alex Makol,
  • Victoria Valdenegro,
  • Félix Acosta,
  • María Soledad Izquierdo and
  • Silvia Torrecillas

28 November 2022

The aim of the present study is to evaluate the potential of two functional additives as gill endogenous antioxidant capacity boosters in European sea-bass juveniles fed low-FM/FO diets when challenged against physical and biological stressors. For t...

  • Article
  • Open Access
30 Citations
7,864 Views
11 Pages

11 April 2021

The technological properties of raw fish are influenced by the changes in protein structure under heating, which determines the texture and quality of the product. The aim of the study was to examine the protein denaturation temperature and the rheol...

  • Article
  • Open Access
9 Citations
2,715 Views
13 Pages

27 April 2024

The systematic revision of the family Peristediidae remains an unresolved issue due to their diverse and unique morphology. Despite the popularity of using mitochondrial genome research to comprehensively understand phylogenetic relationships in fish...

  • Article
  • Open Access
20 Citations
6,394 Views
22 Pages

Socioeconomic Disparities in Diet Vary According to Migration Status among Adolescents in Belgium

  • Manon Rouche,
  • Bart de Clercq,
  • Thérésa Lebacq,
  • Maxim Dierckens,
  • Nathalie Moreau,
  • Lucille Desbouys,
  • Isabelle Godin and
  • Katia Castetbon

10 April 2019

Little information concerning social disparities in adolescent dietary habits is currently available, especially regarding migration status. The aim of the present study was to estimate socioeconomic disparities in dietary habits of school adolescent...

  • Article
  • Open Access
1 Citations
1,678 Views
11 Pages

Mitogenome, Poly(A) Mitotranscriptome, and Molecular Phylogeny of Rasbora rasbora (Family Danionidae; Subfamily Rasborinae)

  • Stanislava Wolf Profant,
  • Tor Erik Jørgensen,
  • Eirik Austad,
  • Igor Babiak and
  • Steinar Daae Johansen

9 August 2024

The subfamily Rasborinae is a species-rich group of freshwater fish related to zebrafish; however, its taxonomy remains unclear. We present the complete mitogenome and corresponding polyadenylated mitotranscriptome of Rasbora rasbora (Hamilton 1822)...

  • Article
  • Open Access
18 Citations
3,450 Views
10 Pages

29 August 2022

Red tilapia eggs one day post fertilization (dpf) were exposed to coenzyme Q10 (CoQ10) at rates of 0, 5, and 10 mg/L for control, treatment 2 (C5), and treatment 3 (C10), respectively, without exchanging water and until the larval mouth-opening stage...

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