Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting
Abstract
:1. Introduction
2. Results
2.1. Identification of Individual Thinopyrum intermedium Chromosomes
2.2. Chromosome Identification of Wheat–Th. intermedium Amphiploids
2.3. Constitution of Th. intermedium Chromosomes in 12 Wheat–Thinopyrum Amphiploids
2.4. Revisiting the Karyotype of Wheat–Th. intermedium Additions Z3
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Probe Preparation
4.3. Fluorescence In Situ Hybridization
4.4. Molecular Marker Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Materials | Chromosome Number | Chromosome Constitution | Figure |
---|---|---|---|
Zhong2 | 2n = 56, four 6A | 1J, 2St-JS, 3St, 4JS, 5JS, 7JS | Figure S2-9 |
Zhong3 | 2n = 56 | 1St-JS, 2JS-St, 3St, 4St, 5J-St, 6JS-J, 7JS | Figure S2-10 |
Zhong4 | 2n = 56 | 1St-JS, 2St-JS, 3St-6St, 4St-, 5J-St, 6JS-J, 7JS | Figure S2-11 |
Zhong5 | 2n = 56 | 1St-JS, 2St-JS, 3St, 4St, 5J-St, 6JS-J, 7JS | Figure S2-12 |
TAI7045 | 2n = 56 | 1St-JS, 2St, 3JS, 4J, 5J-St, 6JS-J, 7JS | Figure S2-3 |
y70-1-4 | 2n = 56 | 1St-JS, 2St, 3JS, 4J, 5J-St (1), 5St (1), 6JS-J, 7JS | Figure S2-8 |
78784 | 2n = 56 | 1St-JS, 2St-JS, 3St, 4St, 5St, 6JS-J, 7JS | Figure S2-1 |
TAI7047 | 2n = 56 + t | 1St-JS, 2St, 3JS, 4J, 5J-St, 6JS-J, 7JS, 7J telo | Figure S2-4 |
78829 | 2n = 56 | 1St-JS, 2St-JS, 3St, 4St, 5J-St, 6JS-J, 7JS | Figure 3 |
TE-1502 | 2n = 56, absence of 4B | 1St, 2St, 3J, 4J, 4JS, 2JS-5JS, 6St, 7St | Figure S2-5 |
TE-1508 | 2n = 56 | 1St, 2JS, 3J, 4J, 4JS, 6St, 7St | Figure S2-6 |
TH101-2 | 2n = 56 | 1St, 2JS, 3St, 4JS, 5JS, 6St, 7St | Figure S2-7 |
8024 | 2n = 54, absence of 7D | 1St-JS, 2St-JS, 3St, 4JS, 5J-St, 6JS-J, 7JS | Figure S2-2 |
TAF46 | 2n = 56 | 1J, 2St-, 3J, 4St, 5J, 6St, 7J | Figure 2 |
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Yu, Z.; Wang, H.; Yang, E.; Li, G.; Yang, Z. Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting. Plants 2022, 11, 2109. https://doi.org/10.3390/plants11162109
Yu Z, Wang H, Yang E, Li G, Yang Z. Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting. Plants. 2022; 11(16):2109. https://doi.org/10.3390/plants11162109
Chicago/Turabian StyleYu, Zhihui, Hongjin Wang, Ennian Yang, Guangrong Li, and Zujun Yang. 2022. "Precise Identification of Chromosome Constitution and Rearrangements in Wheat–Thinopyrum intermedium Derivatives by ND-FISH and Oligo-FISH Painting" Plants 11, no. 16: 2109. https://doi.org/10.3390/plants11162109