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New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds

by Wei Xi 1,2,†, Zongxiang Tang 1,2,†, Shuyao Tang 1,2, Zujun Yang 3, Jie Luo 1,2 and Shulan Fu 1,2,*
1
Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China
2
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China
3
Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(8), 2031; https://doi.org/10.3390/ijms20082031
Received: 4 April 2019 / Revised: 20 April 2019 / Accepted: 22 April 2019 / Published: 25 April 2019
(This article belongs to the Special Issue Molecular Advances in Wheat and Barley)
Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs. View Full-Text
Keywords: wheat; Thinopyrum; chromosome; ND-FISH; oligo probe wheat; Thinopyrum; chromosome; ND-FISH; oligo probe
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MDPI and ACS Style

Xi, W.; Tang, Z.; Tang, S.; Yang, Z.; Luo, J.; Fu, S. New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds. Int. J. Mol. Sci. 2019, 20, 2031.

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