Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization
Abstract
:1. Introduction
2. Results
2.1. Construction of Wheat Karyotype by ND-FISH
2.2. ND-FISH Polymorphisms of A-, B-, and D-Genome Chromosomes
2.3. Detection of Chromosomal Translocations
2.4. Distributions of Different Chromosomal Types between the Two Wheat Germplasm Resources
2.5. Genetic Relationships Revealed by FISH Patterns
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. ND-FISH Analysis
4.3. Data Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Number | Name | No. of Chr | Translocation |
---|---|---|---|
CIMCOG1 | ATTILA | 2n = 42 | |
CIMCOG2 | ATTILA∗2/PBW65 | 2n = 42 | |
CIMCOG3 | ATTILA∗2/PBW65∗2/5/REH/HARE//2∗BCN/3/CROC_1/AE.SQUARROSA (213)//PGO/4/HUITES | 2n = 42 | |
CIMCOG4 | ATTILA//PGO/SERI/3/PASTOR | 2n = 42 | |
CIMCOG5 | BABAX/LR42//BABAX/3/ER2000 | 2n = 42 | |
CIMCOG6 | BABAX/LR42//BABAX/3/VORB | 2n = 28 | |
CIMCOG7 | BACANORA T 88 | 2n = 42 | |
CIMCOG8 | BAVIACORA M 92 | 2n = 42 | 5BS.7BS, 5BL.7BL |
CIMCOG9 | BCN/RIALTO | 2n = 42 | |
CIMCOG10 | BCN/WBLL1 | 2n = 42 | |
CIMCOG11 | BECARD | 2n = 42 | |
CIMCOG12 | BECARD/KACHU | 2n = 42 | |
CIMCOG13 | BRBT1∗2/KIRITATI | 2n = 42 | |
CIMCOG14 | C80.1/3∗BATAVIA//2∗WBLL1/5/REH/HARE//2∗BCN/3/CROC_1/AE.SQUARROSA (213)//PGO/4/HUITES | 2n = 42 | 1RS.1BL |
CIMCOG15 | SAUAL/4/CROC_1/AE.SQUARROSA (205)//KAUZ/3/ATTILA/5/SAUAL | 2n = 42 | |
CIMCOG16 | SAUAL/WHEAR//SAUAL | 2n = 42 | |
CIMCOG17 | CHIR3/4/SIREN//ALTAR 84/AE.SQUARROSA (205)/3/3∗BUC/5/PFAU/WEAVER | 2n = 42 | 1RS.1BL, 5BS.7BS, 5BL.7BL |
CIMCOG18 | CHWL86/6/FILIN/IRENA/5/CNDO/R143//ENTE/MEXI_2/3/AEGILOPS SQUARROSA (TAUS)/4/WEAVER | 2n = 42 | |
CIMCOG19 | CMH79A.955/4/AGA/3/4∗SN64/CNO67//INIA66/5/NAC/6/RIALTO | 2n = 42 | |
CIMCOG20 | CIRNO C 2008 | 2n = 42 | |
CIMCOG21 | CNDO/R143//ENTE/MEXI_2/3/AEGILOPS SQUARROSA (TAUS)/4/OCI/5/PASTOR/6/TEMPORALERA M 87/ROMO96 | 2n = 42 | |
CIMCOG22 | CNDO/R143//ENTE/MEXI_2/3/AEGILOPS SQUARROSA (TAUS)/4/WEAVER/5/2∗KAUZ | 2n = 42 | |
CIMCOG23 | CNO79//PF70354/MUS/3/PASTOR/4/BAV92∗2/5/FH6-1-7 | 2n = 42 | |
CIMCOG24 | CROC_1/AE.SQUARROSA (205)//BORL95/3/PRL/SARA//TSI/VEE#5/4/FRET2 | 2n = 42 | |
CIMCOG25 | GK ARON/AG SECO 7846//2180/4/2∗MILAN/KAUZ//PRINIA/3/BAV92 | 2n = 42 | |
CIMCOG26 | KINGBIRD #1//INQALAB 91∗2/TUKURU | 2n = 28 | |
CIMCOG27 | KFA/3/PFAU/WEAVER//BRAMBLING/4/PFAU/WEAVER∗2//BRAMBLING | 2n = 42 | |
CIMCOG28 | MEX94.27.1.20/3/SOKOLL//ATTILA/3∗BCN | 2n = 42 | |
CIMCOG29 | MILAN/KAUZ//PRINIA/3/BAV92 | 2n = 42 | |
CIMCOG30 | MUNAL #1 | 2n = 42 | |
CIMCOG31 | ATTILA/PASTOR | 2n = 42 | |
CIMCOG32 | OASIS/5∗BORL95/5/CNDO/R143//ENTE/MEXI75/3/AE.SQ/4/2∗OCI | 2n = 42 | |
CIMCOG33 | MISR 1 | 2n = 42 | |
CIMCOG34 | OASIS/SKAUZ//4∗BCN/3/2∗PASTOR/5/FRET2∗2/4/SNI/TRAP#1/3/KAUZ∗2/TRAP//KAUZ/6/SAUAL #1 | 2n = 42 | |
CIMCOG35 | PANDORA//WBLL1∗2/BRAMBLING | 2n = 42 | |
CIMCOG36 | PASTOR/3/URES/JUN//KAUZ/4/WBLL1 | 2n = 42 | |
CIMCOG37 | PAVON F 76 | 2n = 42 | |
CIMCOG38 | PBW343∗2/KUKUNA∗2//FRTL/PIFED | 2n = 42 | 1RS.1BL |
CIMCOG39 | PFAU/SERI.1B//AMAD/3/WAXWING | 2n = 42 | |
CIMCOG40 | ARMENT//2∗SOOTY_9/RASCON_37/4/CNDO/PRIMADUR//HAI-OU_17/3/SNITAN | 2n = 42 | |
CIMCOG41 | QUAIU #3//MILAN/AMSEL | 2n = 42 | |
CIMCOG42 | RL6043/4∗NAC//2∗PASTOR | 2n = 42 | 5BS.7BS, 5BL.7BL |
CIMCOG43 | ROLF07∗2/5/REH/HARE//2∗BCN/3/CROC_1/AE.SQUARROSA (213)//PGO/4/HUITES | 2n = 42 | 1RS.1BL |
CIMCOG44 | SERI M 82 | 2n = 42 | |
CIMCOG45 | SIETE CERROS T66 | 2n = 42 | 1RS.1BL |
CIMCOG46 | SOKOLL∗2/3/BABAX/LR42//BABAX | 2n = 42 | 1RS.1BL |
CIMCOG47 | SOKOLL//PBW343∗2/KUKUNA/3/ATTILA/PASTOR | 2n = 42 | |
CIMCOG48 | TACUPETO F2001/SAUAL/4/BABAX/LR42//BABAX∗2/3/KURUKU | 2n = 42 | |
CIMCOG49 | TACUPETO F2001/BRAMBLING∗2//KACHU | 2n = 42 | |
CIMCOG50 | TC870344/GUI//TEMPORALERA M 87/AGR/3/2∗WBLL1 | 2n = 42 | |
CIMCOG51 | TRAP#1/BOW/3/VEE/PJN//2∗TUI/4/BAV92/RAYON/5/KACHU #1 | 2n = 42 | |
CIMCOG52 | TRCH/SRTU//KACHU | 2n = 42 | |
CIMCOG53 | UP2338∗2/4/SNI/TRAP#1/3/KAUZ∗2/TRAP//KAUZ/5/MILAN/KAUZ//CHIL/CHUM18/6/UP2338∗2/4/SNI/TRAP#1/3/KAUZ∗2/TRAP//KAUZ | 2n = 42 | 5BS.7BS, 5BL.7BL |
CIMCOG54 | W15.92/4/PASTOR//HXL7573/2∗BAU/3/WBLL1 | 2n = 42 | |
CIMCOG55 | BECARD | 2n = 42 | |
CIMCOG56 | WBLL1∗2/4/BABAX/LR42//BABAX/3/BABAX/LR42//BABAX | 2n = 42 | |
CIMCOG57 | WBLL1∗2/KURUKU∗2/5/REH/HARE//2∗BCN/3/CROC_1/AE.SQUARROSA (213)//PGO/4/HUITES | 2n = 42 | |
CIMCOG58 | WBLL1∗2/TUKURU∗2/4/CROC_1/AE.SQUARROSA (205)//BORL95/3/2∗MILAN | 2n = 42 | |
CIMCOG59 | WHEAR/SOKOLL | 2n = 42 | 1RS.1BL, 5BS.7BS, 5BL.7BL |
CIMCOG60 | YAV_3/SCO//JO69/CRA/3/YAV79/4/AE.SQUARROSA (498)/5/LINE 1073/6/KAUZ∗2/4/CAR//KAL/BB/3/NAC/5/KAUZ/7/KRONSTAD F2004/8/KAUZ/PASTOR//PBW343 | 2n = 42 |
Number | Name | Variety Type | No. of Chr | Translocation | Number | Name | Variety Type | No. of Chr | Translocation |
---|---|---|---|---|---|---|---|---|---|
CACG1 | Daqingmai | landrace | 2n = 42 | CACG48 | Baihuomia | landrace | 2n = 42 | ||
CACG2 | Guangmai | landrace | 2n = 42 | CACG49 | Sanyuehuang | landrace | 2n = 42 | ||
CACG3 | Xinkehan9 | breeding variety | 2n = 42 | CACG50 | Hongqiangchang | landrace | 2n = 42 | ||
CACG4 | Kefeng3 | breeding variety | 2n = 42 | 2AS.5AS-5AL/5AS.2AS-2AL | CACG51 | Quzimai | landrace | 2n = 42 | |
CACG5 | Kelao4 | breeding variety | 2n = 42 | CACG52 | Pingyuan50 | breeding variety | 2n = 42 | ||
CACG6 | Xinshuguang1 | breeding variety | 2n = 42 | 1AS.4BS, 1AL.4BL | CACG53 | Baibiansui | landrace | 2n = 42 | |
CACG7 | Dongnong101 | breeding variety | 2n = 42 | CACG54 | Gejiaxiang | landrace | 2n = 42 | ||
CACG8 | Xinshuguang6 | breeding variety | 2n = 42 | CACG55 | Dachunbaisilengmai | landrace | 2n = 42 | ||
CACG9 | Jichun1016 | breeding variety | 2n = 42 | CACG56 | Zhahong | landrace | 2n = 42 | ||
CACG10 | Chixiaomai | landrace | 2n = 42 | CACG57 | Motuoxiaomai | landrace | 2n = 42 | ||
CACG11 | st2422/464 | introduced variety | 2n = 42 | CACG58 | Bianbachunmai-6 | landrace | 2n = 42 | ||
CACG12 | Orofen | introduced variety | 2n = 42 | 3BS.4AL, 3BL.4AS | CACG59 | Baimangxiaomai | landrace | 2n = 42 | |
CACG13 | Nonglin10 | introduced variety | 2n = 42 | CACG60 | Wujiangzhuo | landrace | 2n = 42 | ||
CACG14 | introduced variety | 2n = 42 | CACG61 | Kangdingxiaomai | breeding variety | 2n = 42 | |||
CACG15 | Early premiun | introduced variety | 2n = 42 | CACG62 | Zangdong4 | breeding variety | 2n = 42 | ||
CACG16 | Triumph | introduced variety | 2n = 42 | CACG63 | Rikaze54 | breeding variety | 2n = 42 | ||
CACG17 | Lovrin | introduced variety | 2n = 42 | 1RS.1BL | CACG64 | Rikaze8 | breeding variety | 2n = 42 | |
CACG18 | introduced variety | 2n = 42 | CACG65 | Yizhimai | landrace | 2n = 42 | |||
CACG19 | Tanori | introduced variety | 2n = 42 | CACG66 | Dabaimai | landrace | 2n = 42 | ||
CACG20 | Atlas66 | introduced variety | 2n = 42 | CACG67 | Laohan | landrace | 2n = 42 | ||
CACG21 | Gansu96 | breeding variety | 2n = 42 | CACG68 | Huoliyan | landrace | 2n = 42 | 4BS.2AS, 4BL.2AL | |
CACG22 | Chaoanxiaomai | landrace | 2n = 42 | CACG69 | Shanmai | landrace | 2n = 42 | 4AS.4BL, 4AL.4BS | |
CACG23 | Chike | landrace | 2n = 42 | 2BL.4DS, 2BS.4DL | CACG70 | Hongtuzi | landrace | 2n = 42 | |
CACG24 | Songruimai4 | breeding variety | 2n = 42 | CACG71 | Baidatou | landrace | 2n = 42 | ||
CACG25 | Shengen | landrace | 2n = 42 | CACG72 | Jinhuangmai | landrace | 2n = 42 | ||
CACG26 | Shanglinxiaomai | landrace | 2n = 42 | CACG73 | Huzhuhong | landrace | 2n = 42 | ||
CACG27 | Pingyang27 | breeding variety | 2n = 42 | CACG74 | Jinmai4 | breeding variety | 2n = 42 | ||
CACG28 | Jinan2 | breeding variety | 2n = 42 | 1DL.4AL, 1DS.4AS | CACG75 | Dingxi24 | breeding variety | 2n = 42 | |
CACG29 | Qubao | landrace | 2n = 42 | CACG76 | Ning10 | breeding variety | 2n = 42 | ||
CACG30 | Bainong3217 | breeding variety | 2n = 42 | CACG77 | Fan6 | breeding variety | 2n = 42 | ||
CACG31 | Yannong15 | breeding variety | 2n = 42 | CACG78 | Guinong10 | breeding variety | 2n = 42 | 1RS.1BL | |
CACG32 | Xinong6028 | breeding variety | 2n = 42 | CACG79 | Yunmai34 | breeding variety | 2n = 42 | 1RS.1BL | |
CACG33 | Jibei2 | breeding variety | 2n = 42 | 1RS.1BL | CACG80 | Xingyi4 | breeding variety | 2n = 42 | 1RS.1BL |
CACG34 | Neichan5 | breeding variety | 2n = 42 | CACG81 | Fengmai11 | breeding variety | 2n = 42 | ||
CACG35 | Zhengzhou6 | breeding variety | 2n = 42 | CACG82 | Tongjiabaxiaomai | landrace | 2n = 42 | ||
CACG36 | Jinan17 | breeding variety | 2n = 42 | CACG83 | Honghuamai | landrace | 2n = 42 | ||
CACG37 | Shannong7859 | breeding variety | 2n = 42 | 1RS.1BL | CACG84 | Baimaizi | landrace | 2n = 42 | 5BS.2DS, 5BL.2DL |
CACG38 | Aifeng3 | breeding variety | 2n = 42 | CACG85 | Chengduguangtou | landrace | 2n = 42 | ||
CACG39 | Lumai1 | breeding variety | 2n = 42 | 1RS.7DS, 1BL.7DL | CACG86 | Jiangmai | landrace | 2n = 42 | |
CACG40 | Wenmai6 | breeding variety | 2n = 42 | CACG87 | Baihuamai | landrace | 2n = 42 | ||
CACG41 | Laizhou953 | breeding variety | 2n = 42 | 1RS.1BL | CACG88 | Huanxiangmai | landrace | 2n = 42 | |
CACG42 | Baimangmai | landrace | 2n = 42 | CACG89 | Hanzhongbai | landrace | 2n = 42 | ||
CACG43 | Huangguaxian | landrace | 2n = 42 | CACG90 | Xiaosanyuehuang | landrace | 2n = 42 | ||
CACG44 | Banjiemang | landrace | 2n = 42 | CACG91 | Suotiaohongmai | landrace | 2n = 42 | ||
CACG45 | Quanguding | landrace | 2n = 42 | CACG92 | Hongxumai | landrace | 2n = 42 | ||
CACG46 | Xishanbiansui | landrace | 2n = 42 | CACG93 | Zimai | landrace | 2n = 42 | ||
CACG47 | Honggoudou | landrace | 2n = 42 | CACG94 | Chinese Spring | landrace | 2n = 42 | Reference genome |
Chr | No. of Types | Type | CIMCOG Wheat Lines | CACG Wheat Lines | Chr | No. of Types | Type | CIMCOG Wheat Lines | CACG Wheat Lines | Chr | No. of Types | Type | CIMCOG Wheat Lines | CACG Wheat Lines | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Lines | Percent (%) | No. of Lines | Percent (%) | No. of Lines | Percent (%) | No. of Lines | Percent (%) | No. of Lines | Percent (%) | No. of Lines | Percent (%) | |||||||||
1A | 2 | I | 48 | 80.00 | 1B | 8 | I | 2 | 3.33 | 1D | 8 | I | 27 | 45.00 | 60 | 64.52 | ||||
II | 12 | 20.00 | 92 | 98.92 | II | 5 | 8.33 | 41 | 44.09 | II | 21 | 35.00 | 6 | 6.45 | ||||||
2A | 13 | I | 13 | 21.67 | III | 35 | 58.33 | 8 | 8.60 | III | 5 | 8.33 | 3 | 3.23 | ||||||
II | 10 | 16.67 | 14 | 15.05 | IV | 11 | 18.33 | 24 | 25.81 | IV | 5 | 8.33 | 4 | 4.30 | ||||||
III | 34 | 56.67 | 49 | 52.69 | V | 2 | 2.15 | V | 16 | 17.20 | ||||||||||
IV | 2 | 3.33 | 4 | 4.30 | VI | 4 | 4.30 | VI | 1 | 1.08 | ||||||||||
V | 1 | 1.67 | 3 | 3.23 | VII | 4 | 4.30 | VII | 1 | 1.08 | ||||||||||
VI | 2 | 2.15 | VIII | 2 | 2.15 | VIII | 1 | 1.08 | ||||||||||||
VII | 2 | 2.15 | 2B | 10 | I | 6 | 10.00 | 2D | 5 | I | 1 | 1.67 | ||||||||
VIII | 4 | 4.30 | II | 19 | 31.67 | 5 | 5.38 | II | 14 | 23.33 | 24 | 25.81 | ||||||||
IX | 4 | 4.30 | III | 12 | 20.00 | 24 | 25.81 | III | 40 | 66.67 | 51 | 54.84 | ||||||||
X | 4 | 4.30 | IV | 11 | 18.33 | 7 | 7.53 | IV | 3 | 5.00 | 3 | 3.23 | ||||||||
XI | 1 | 1.08 | V | 12 | 20.00 | 2 | 2.15 | V | 14 | 15.05 | ||||||||||
XII | 2 | 2.15 | VI | 19 | 20.43 | 3D | 4 | I | 4 | 6.67 | ||||||||||
XIII | 2 | 2.15 | VII | 7 | 7.53 | II | 42 | 70.00 | 37 | 39.78 | ||||||||||
3A | 4 | I | 60 | 100.00 | 80 | 86.02 | VIII | 8 | 8.60 | III | 11 | 18.33 | 55 | 59.14 | ||||||
II | 7 | 7.53 | IX | 17 | 18.28 | IV | 1 | 1.67 | 1 | 1.08 | ||||||||||
III | 5 | 5.38 | X | 3 | 3.23 | 4D | 4 | I | 6 | 10.00 | ||||||||||
IV | 1 | 1.08 | 3B | 12 | I | 2 | 3.33 | II | 47 | 78.33 | 48 | 51.61 | ||||||||
4A | 10 | I | 20 | 33.33 | II | 16 | 26.67 | 8 | 8.60 | III | 5 | 8.33 | 43 | 46.24 | ||||||
II | 24 | 40.00 | III | 25 | 41.67 | 6 | 6.45 | IV | 1 | 1.08 | ||||||||||
III | 1 | 1.67 | IV | 6 | 10.00 | 4 | 4.30 | 5D | 3 | I | 50 | 83.33 | 17 | 18.28 | ||||||
IV | 1 | 1.67 | V | 3 | 5.00 | 21 | 22.58 | II | 5 | 8.33 | 33 | 35.48 | ||||||||
V | 1 | 1.67 | 6 | 6.45 | VI | 5 | 8.33 | 10 | 10.75 | III | 3 | 5.00 | 43 | 46.24 | ||||||
VI | 7 | 11.67 | 22 | 23.66 | VII | 3 | 5.00 | 3 | 3.23 | 6D | 3 | I | 58 | 96.67 | 77 | 82.80 | ||||
VII | 4 | 6.67 | 6 | 6.45 | VIII | 6 | 6.45 | II | 10 | 10.75 | ||||||||||
VIII | 2 | 3.33 | 24 | 25.81 | IX | 3 | 3.23 | III | 6 | 6.45 | ||||||||||
IX | 31 | 33.33 | X | 10 | 10.75 | 7D | 4 | I | 4 | 6.67 | ||||||||||
X | 1 | 1.08 | XI | 13 | 13.98 | II | 46 | 76.67 | 81 | 87.10 | ||||||||||
5A | 9 | I | 6 | 10.00 | XII | 8 | 8.60 | III | 8 | 13.33 | 10 | 10.75 | ||||||||
II | 4 | 6.67 | 4B | 5 | I | 48 | 80.00 | 44 | 47.31 | IV | 1 | 1.08 | ||||||||
III | 12 | 20.00 | 8 | 8.60 | II | 10 | 16.67 | 37 | 39.78 | 5BS/7BS | 1 | I | 5 | 8.33 | ||||||
IV | 31 | 51.67 | 39 | 41.94 | III | 2 | 3.33 | 1 | 1.08 | 5BL/7BL | 4 | I | 2 | 3.33 | ||||||
V | 4 | 6.67 | 7 | 7.53 | IV | 7 | 7.53 | II | 1 | 1.67 | ||||||||||
VI | 3 | 5.00 | 22 | 23.66 | V | 1 | 1.08 | III | 1 | 1.67 | ||||||||||
VII | 6 | 6.45 | 5B | 6 | I | 2 | 3.33 | IV | 1 | 1.67 | ||||||||||
VIII | 9 | 9.68 | II | 36 | 60.00 | 30 | 32.26 | 1RS/1BL | 1 | I | 7 | 11.67 | 7 | 7.53 | ||||||
IX | 1 | 1.08 | III | 15 | 25.00 | 23 | 24.73 | 1RS.7DS, 1BL.7DL | 1 | 1.08 | ||||||||||
6A | 2 | I | 46 | 76.67 | 31 | 33.33 | IV | 1 | 1.67 | 2 | 2.15 | 2AS.5AS-5AL/5AS.2AS-2AL | 1 | 1.08 | ||||||
II | 14 | 23.33 | 62 | 66.67 | V | 1 | 1.67 | 4 | 4.30 | 1AS.4BS, 1AL.4BL | 1 | 1.08 | ||||||||
7A | 7 | I | 38 | 63.33 | 22 | 23.66 | VI | 33 | 35.48 | 3BS.4AL, 3BL.4AS | 1 | 1.08 | ||||||||
II | 10 | 16.67 | 14 | 15.05 | 6B | 13 | I | 7 | 11.67 | 4BS.2AS, 4BL.2AL | 1 | 1.08 | ||||||||
III | 8 | 13.33 | 4 | 4.30 | II | 1 | 1.67 | 4AS.4BL,4AL.4BS | 1 | 1.08 | ||||||||||
IV | 4 | 6.67 | 30 | 32.26 | III | 1 | 1.67 | 2BL.4DS, 2BS.4DL | 1 | 1.08 | ||||||||||
V | 15 | 16.13 | IV | 31 | 51.67 | 20 | 21.51 | 5BS.2DS, 5BL.2DL | 1 | 1.08 | ||||||||||
VI | 3 | 3.23 | V | 14 | 23.33 | 10 | 10.75 | 1DL.4AL, 1DS.4AS | 1 | 1.08 | ||||||||||
VII | 5 | 5.38 | VI | 2 | 3.33 | 3 | 3.23 | |||||||||||||
VII | 2 | 3.33 | 12 | 12.90 | ||||||||||||||||
VIII | 2 | 3.33 | 24 | 25.81 | ||||||||||||||||
IX | 7 | 7.53 | ||||||||||||||||||
X | 9 | 9.68 | ||||||||||||||||||
XI | 6 | 6.45 | ||||||||||||||||||
XII | 1 | 1.08 | ||||||||||||||||||
XIII | 1 | 1.08 | ||||||||||||||||||
7B | 11 | I | 7 | 11.67 | ||||||||||||||||
II | 3 | 5.00 | ||||||||||||||||||
III | 3 | 5.00 | ||||||||||||||||||
IV | 20 | 33.33 | 5 | 5.38 | ||||||||||||||||
V | 15 | 25.00 | 44 | 47.31 | ||||||||||||||||
VI | 1 | 1.67 | 10 | 10.75 | ||||||||||||||||
VII | 1 | 1.67 | 20 | 21.51 | ||||||||||||||||
VIII | 1 | 1.67 | 4 | 4.30 | ||||||||||||||||
IX | 4 | 6.67 | 5 | 5.38 | ||||||||||||||||
X | 3 | 3.23 | ||||||||||||||||||
XI | 2 | 2.15 |
Name of Probe | Sequence and Fluorochrome Label |
---|---|
Oligo-pSc119.2-1 | 6-FAM-5′CCGTT TTGTG GACTA TTACT CACCG CTTTG GGGTC CCATA GCTAT3′ |
Oligo-pTa535-1 | Tamra-5′AAAAA CTTGA CGCAC GTCAC GTACA AATTG GACAA ACTCT TTCGG AGTAT CAGGG TTTC3′ |
Oligo-(GAA)7 | Cy5-5′GAAGAAGAAGAAGAAGAAGAA3′ |
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Yang, M.; Yang, Z.; Yang, W.; Yang, E. Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization. Plants 2022, 11, 1403. https://doi.org/10.3390/plants11111403
Yang M, Yang Z, Yang W, Yang E. Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization. Plants. 2022; 11(11):1403. https://doi.org/10.3390/plants11111403
Chicago/Turabian StyleYang, Manyu, Zujun Yang, Wuyun Yang, and Ennian Yang. 2022. "Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization" Plants 11, no. 11: 1403. https://doi.org/10.3390/plants11111403