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Article

The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH

by 1,2,*,†, 2,3,†, 1,2, 1,2, 1,2, 1,2, 1,2, 2,3, 1,2 and 2,3,*
1
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China
2
Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu 611130, Sichuan, China
3
College of Life Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, China
*
Authors to whom correspondence should be addressed.
These authors have contributed equally to this work.
Molecules 2019, 24(6), 1126; https://doi.org/10.3390/molecules24061126
Received: 3 March 2019 / Revised: 15 March 2019 / Accepted: 19 March 2019 / Published: 21 March 2019
Non-denaturing fluorescence in situ hybridization (ND-FISH) has been used to distinguish wheat chromosomes and to detect alien chromosomes in the wheat genome. In this study, five different oligonucleotide probes were used with ND-FISH to examine 21 wheat cultivars and lines. These oligonucleotide probes distinguished 42 wheat chromosomes and also detected rye chromatin in the wheat genome. Moreover, the signal patterns of the oligonucleotide probes Oligo-pTa535-1 and Oligo-pSc119.2-1 showed high polymorphism in the wheat chromosomes. A total of 17.6% of the A group chromosomes, 25.9% of the B group chromosomes and 8.9% of the D group chromosomes showed obvious mutations when they were compared to the standard ND-FISH signal patterns, and most of them were Oligo-pSc119.2-1 mutants. The results suggested that these polymorphisms could be induced by the crossing of wheat cultivars. The results provided more information for the further application of oligonucleotide probes and ND-FISH. View Full-Text
Keywords: ND-FISH; Oligo-pTa535-1; Oligo-pSc119.2-1; chromosome; wheat; mutant ND-FISH; Oligo-pTa535-1; Oligo-pSc119.2-1; chromosome; wheat; mutant
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MDPI and ACS Style

Ren, T.; He, M.; Sun, Z.; Tan, F.; Luo, P.; Tang, Z.; Fu, S.; Yan, B.; Ren, Z.; Li, Z. The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH. Molecules 2019, 24, 1126. https://doi.org/10.3390/molecules24061126

AMA Style

Ren T, He M, Sun Z, Tan F, Luo P, Tang Z, Fu S, Yan B, Ren Z, Li Z. The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH. Molecules. 2019; 24(6):1126. https://doi.org/10.3390/molecules24061126

Chicago/Turabian Style

Ren, Tianheng, Maojie He, Zixin Sun, Feiquan Tan, Peigao Luo, Zongxiang Tang, Shulan Fu, Benju Yan, Zhenglong Ren, and Zhi Li. 2019. "The Polymorphisms of Oligonucleotide Probes in Wheat Cultivars Determined by ND-FISH" Molecules 24, no. 6: 1126. https://doi.org/10.3390/molecules24061126

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