Next Article in Journal
Insect Antifeedant Components of Senecio fistulosus var. fistulosus—Hualtata
Previous Article in Journal
Reactive Oxygen Species as Potential Drivers of the Seed Aging Process
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle

Diversified Chromosome Rearrangements Detected in a Wheat‒Dasypyrum breviaristatum Substitution Line Induced by Gamma-Ray Irradiation

Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 611731, China
*
Author to whom correspondence should be addressed.
Plants 2019, 8(6), 175; https://doi.org/10.3390/plants8060175
Received: 19 May 2019 / Revised: 9 June 2019 / Accepted: 13 June 2019 / Published: 14 June 2019
  |  
PDF [2191 KB, uploaded 14 June 2019]
  |  

Abstract

To determine the composition of chromosome aberrations in a wheat‒Dasypyrum breviaristatum substitution line with seeds treated by a dose of gamma-rays (200 Gy), sequential non-denaturing fluorescence in situ hybridization (ND-FISH) with multiple oligonucleotide probes was used to screen individual plants of the mutagenized progenies. We identified 122 types of chromosome rearrangements, including centromeric, telomeric, and intercalary chromosome translocations from a total of 772 M1 and 872 M2 plants. The frequency of reciprocal translocations between B- and D-chromosomes was higher than that between A- and D-chromosomes. Eight translocations between D. breviaristatum and wheat chromosomes were also detected. The 13 stable plants with multiple chromosome translocations displayed novel agronomic traits. The newly developed materials will enhance wheat breeding programs through wheat‒Dasypyrum introgression and also facilitate future studies on the genetic and epigenetic effects of translocations in wheat genomics. View Full-Text
Keywords: chromosome rearrangement; fluorescence in situ hybridization; gamma ray radiation; wheat‒Dasypyrum substitution chromosome rearrangement; fluorescence in situ hybridization; gamma ray radiation; wheat‒Dasypyrum substitution
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Wang, H.; Yu, Z.; Li, G.; Yang, Z. Diversified Chromosome Rearrangements Detected in a Wheat‒Dasypyrum breviaristatum Substitution Line Induced by Gamma-Ray Irradiation. Plants 2019, 8, 175.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top