Journal of Xenobiotics

17 pages, 6618 KB

Open AccessArticle

Sustainable Biochar–Alumina Composites for Electroanalytical Sensing of Herbicide and Antibiotic

by Nataša Jović-Jovičić, Tatjana Novaković, Tanja Barudžija, Marija Ajduković, Natalia Czerwinska, Chiara Giosuè and Zorica Mojović

J. Xenobiot. 2025, 15(6), 191; https://doi.org/10.3390/jox15060191 - 10 Nov 2025

Abstract

The problem of water pollution by various xenobiotics has gained a lot of interest due to their persistence, bioaccumulation potential, and toxic effects on ecosystems and humans. Electrochemical sensors offer a rapid, sensitive, and cost-effective method for on-site monitoring. In this research, an [...] Read more.

The problem of water pollution by various xenobiotics has gained a lot of interest due to their persistence, bioaccumulation potential, and toxic effects on ecosystems and humans. Electrochemical sensors offer a rapid, sensitive, and cost-effective method for on-site monitoring. In this research, an electrochemical sensor for xenobiotics based on a biochar–alumina composite is developed. The biochar–alumina composites were obtained by the air-limited pyrolysis of oak sawdust in the presence of alumina. Two types of alumina were mixed with oak sawdust in three ratios and subjected to thermal treatment. The resulting composites were characterized by SEM, N₂ adsorption isotherm, XRD, and electrochemical characterization. The detection of the herbicide pendimethalin and the antibiotic ciprofloxacin was investigated, and the composite with the optimal biochar/alumina ratio was selected for each of the xenobiotics studied. A linear current response was obtained for pendimethalin in the concentration range 0.7 μM to 70.0 μM with an LOD of 0.5 μM. A linear current response was obtained for ciprofloxacin in the concentration range 1.6 μM to 55.4 μM with an LOD of 0.63 μM. A comparison of the characterization results with the electroanalytical performance implied the importance of the hydrophobic/hydrophilic nature of the electrode surface for detecting the analyte under investigation. Full article

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25 pages, 1241 KB

Open AccessReview

A Double Challenge for Fish: The Combined Stress of Warming and Pharmaceuticals in Aquatic Systems

by Tiago Lourenço, Maria João Rocha, Eduardo Rocha and Tânia Vieira Madureira

J. Xenobiot. 2025, 15(6), 190; https://doi.org/10.3390/jox15060190 (registering DOI) - 8 Nov 2025

Abstract

Aquatic ecosystems are increasingly threatened by multiple anthropogenic stressors, notably climate change and pollution by pharmaceuticals. Global warming is predicted to raise water temperatures by 2–5 °C by the end of the century. As ectotherms, fish are particularly vulnerable due to limited thermal [...] Read more.

Aquatic ecosystems are increasingly threatened by multiple anthropogenic stressors, notably climate change and pollution by pharmaceuticals. Global warming is predicted to raise water temperatures by 2–5 °C by the end of the century. As ectotherms, fish are particularly vulnerable due to limited thermal tolerance and temperature-dependent physiology. Pharmaceuticals are introduced into aquatic systems at concentrations ranging from ng·L⁻¹ to µg·L⁻¹, including widely prescribed classes such as antibiotics, hormones, analgesics, antifungals, and neuropsychiatric drugs. This narrative review synthesizes experimental evidence on the interactive effects of warming and pharmaceutical exposure in fish. Thirty-nine peer-reviewed studies published since 2005 were analyzed. The findings indicate that higher temperatures often exacerbate pharmaceutical-induced toxicity, altering oxidative stress, metabolism, reproduction, and behavior. Antibiotic-focused studies showed temperature-dependent acceleration of absorption, distribution, metabolism, and excretion, with shorter half-lives and reduced tissue persistence at higher temperatures. Estrogenic hormones and antifungals have been shown to interact with thermal regimes, disrupting reproductive physiology and skewing sex ratios, particularly in species exhibiting temperature-dependent sex determination. Neuropsychiatric drugs exhibited altered uptake and metabolism under warming conditions, resulting in increased brain bioaccumulation and behavioral alterations affecting ecological fitness. Analgesics and anti-inflammatories remain understudied despite their widespread use, with evidence suggesting synergistic effects on oxidative stress at elevated temperatures. Significant research gaps persist regarding chronic exposures, early developmental stages, ecologically relevant temperature scenarios, and underrepresented or absent drug classes, such as hypolipidemic drugs. Ultimately, broader and integrated approaches are needed to better understand and predict the ecological risks of pharmaceutical pollution in a warming world. Full article

(This article belongs to the Topic Aquatic Emerging Contaminants and Their Ecotoxicological Consequences, 2nd Edition)

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26 pages, 3817 KB

Open AccessArticle

Low Concentrations of Sulfoxaflor Do Not Adversely Affect mRNA Levels in Various Testicular Cells When Administered to Either Mature or Immature Mice

by Hayato Terayama, Kenta Nagahori, Daisuke Kiyoshima, Tsutomu Sato, Yoko Ueda, Masahito Yamamoto, Kaori Suyama, Tomoko Tanaka, Midori Yamamoto, Akifumi Eguchi, Emiko Todaka, Kenichi Sakurai, Shogo Hayashi, Haruki Yamada and Kou Sakabe

J. Xenobiot. 2025, 15(6), 189; https://doi.org/10.3390/jox15060189 - 7 Nov 2025

Abstract

Sulfoxaflor, an insecticide, acts on nicotinic acetylcholine receptors. It has a functional group similar to that of neonicotinoid insecticides, which are testicular toxicants. Recently, the adverse effects of sulfoxaflor on the testes have been reported in rats. This study aimed to address the [...] Read more.

Sulfoxaflor, an insecticide, acts on nicotinic acetylcholine receptors. It has a functional group similar to that of neonicotinoid insecticides, which are testicular toxicants. Recently, the adverse effects of sulfoxaflor on the testes have been reported in rats. This study aimed to address the lack of reports on sulfoxaflor administration in mice and its effects on the testes. ICR mice (3- and 10-week-old) were treated ad libitum with two different concentrations (10 and 100 mg/kg) of sulfoxaflor for 4 and 8 weeks. Histological analysis and real-time reverse transcription polymerase chain reaction were performed. Testis weights relative to body weights in the sulfoxaflor groups showed no significant difference compared to the control group. Testicular tissue in the sulfoxaflor groups was unchanged compared to that in the control group. The sulfoxaflor-treated group showed no significant differences in the mRNA expression of luteinizing hormone and follicle-stimulating hormone in the pituitary gland compared to the control group. Furthermore, no significant differences were noted in the mRNA expression levels of various gene markers in the testes between the sulfoxaflor-treated and control groups. These markers include those related to Leydig cells, testosterone synthesis, Sertoli cells, proliferating cells, meiotic cells, pachytene spermatocytes, round spermatids, apoptotic cells, antioxidant enzymes, oxidative stress factors, and mitochondrial function. In contrast to findings in rats, which showed testicular toxicity, sulfoxaflor administration at low concentrations did not adversely affect intratesticular cells in either mature or immature mice at the doses and time points examined. In the future, we would like to conduct research on high concentrations of sulfoxaflor by changing the administration method. Full article

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30 pages, 2070 KB

Open AccessReview

Treatment of Xenobiotic Cyclic Nitramine Explosives in Wastewater

by Swati Gupta and Zeev Ronen

J. Xenobiot. 2025, 15(6), 188; https://doi.org/10.3390/jox15060188 - 7 Nov 2025

Abstract

Cyclic nitramine explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) are xenobiotics that are utilized in a variety of propellants and traditional weapons. The primary source of water contamination is the industrial use of these hazardous substances in propellants and wastewater [...] Read more.

Cyclic nitramine explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) are xenobiotics that are utilized in a variety of propellants and traditional weapons. The primary source of water contamination is the industrial use of these hazardous substances in propellants and wastewater generated from munitions production facilities. These chemicals have a negative impact on human health and ecosystems. It is necessary to remove these toxic compounds from the environment safely because their production and usage have seriously contaminated soil and groundwater. Although there are no widely adopted WHO or US federal Maximum Contaminant Levels (MCLs) for military explosives, the health advisory limits for RDX in drinking water are 2 µg/L, and for HMX are 400 µg/L. Numerous traditional treatment approaches that incorporate physical, biological, and chemical processes have been used to decontaminate explosive wastewater. However, contaminants are not completely mineralized by these methods. Complete reduction of these chemicals can be accomplished by combining suitable methods. For the remediation of explosive effluent, integrated treatment systems that combine the effectiveness of biological and physical-chemical methods have shown promising results. This review discusses the toxicity and some physical–chemical–biological and combined treatment processes of wastewater polluted by these explosive contaminants. Full article

(This article belongs to the Section Ecotoxicology)

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45 pages, 827 KB

Open AccessReview

Global Evidence on Monitoring Human Pesticide Exposure

by Tatiane Renata Fagundes, Carolina Coradi, Beatriz Geovana Leite Vacario, Juliana Maria Bitencourt de Morais Valentim and Carolina Panis

J. Xenobiot. 2025, 15(6), 187; https://doi.org/10.3390/jox15060187 - 7 Nov 2025

Abstract

This study analyzes global data on human exposure to pesticides, focusing on glyphosate, POPs, carbamates, and organophosphates, which are among the most widely used in agricultural and urban environments, providing an overview of global human contamination by these substances. Current research has increasingly [...] Read more.

This study analyzes global data on human exposure to pesticides, focusing on glyphosate, POPs, carbamates, and organophosphates, which are among the most widely used in agricultural and urban environments, providing an overview of global human contamination by these substances. Current research has increasingly focused on the unintended consequences of pesticide use, including food, water, and soil contamination, biodiversity loss (especially beneficial insects such as pollinators), and the growing evidence of adverse impacts on human health (neurological, reproductive, endocrine, and carcinogenic effects). Therefore, we compiled information from several existing studies that evaluated pesticide residues in human biological samples, specifically urine, blood, and breast milk, to assess the extent of exposure. The analysis takes a global perspective, highlighting the importance of monitoring exposure in countries that demonstrate exceptionally high pesticide use (in terms of absolute volume), such as Brazil, the United States, and China, which are among the largest global consumers. The data cover both contemporary pesticides, whose consumption is driven by intensive agriculture in these and other countries, and persistent legacy compounds (POPs) that continue to circulate in nature and accumulate in the human body decades after their ban in many countries. Globally, there is a wide disparity in global regulations, and many developing countries continue to use pesticides that have been banned or severely restricted in more developed nations. Finally, it provides a critical overview of global data on human pesticide contamination. The data reinforce the critical importance of establishing preventive initiatives and strengthening surveillance and monitoring systems to detect and control pesticide residues in human populations globally, ultimately aiming to mitigate the harms of chronic pesticide exposure to human health and well-being. Full article

(This article belongs to the Special Issue New Challenges in the Monitoring, Risk Assessment and Management of Pesticides and Biocides in the “One Health Era”)

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16 pages, 1306 KB

Open AccessReview

Microplastic Polymer Mass Fractions in Marine Bivalves: From Isolation to Hazard Risk

by Tanja Bogdanović, Irena Listeš, Jennifer Gjerde, Sandra Petričević, Zvonimir Jažo, Eddy Listeš, Jelka Pleadin, Darja Sokolić, Ivona Jadrešin and Federica di Giacinto

J. Xenobiot. 2025, 15(6), 186; https://doi.org/10.3390/jox15060186 - 6 Nov 2025

Abstract

Microplastics (MPs) are a ubiquitous marine pollutant, and their presence in bivalves is receiving increasing attention due to the associated risks to human health. The steps of pretreatment, detection, and quantification in the analysis of MPs depend on the type of polymer. Research [...] Read more.

Microplastics (MPs) are a ubiquitous marine pollutant, and their presence in bivalves is receiving increasing attention due to the associated risks to human health. The steps of pretreatment, detection, and quantification in the analysis of MPs depend on the type of polymer. Research on MPs is challenging because of the varying characteristics of these materials, such as the size, shape, and polymer type. Consequently, there are no standardized methods for their collection, separation, identification, or quantification. This review specifically examines the available bivalve digestion steps, focusing on efficient and time-reducing methods, such as the microwave-assisted (MAW) procedure and its advantages. Recent achievements in the application of pyrolysis gas chromatography–mass spectrometry (Pyr-GC-MS) are presented for the profiling of polymer mass-related microplastics data in marine bivalves. Here, we provide an overview of the abundance, properties, and polymer types of MPs in bivalve species, highlighting the polymer mass fractions. To date, the available mass-based concentrations have revealed nine types of MPs—polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polyethylene terephthalate (PET), polystyrene (PS), polymethyl methacrylate (PMMA), polyamide 66 (PA66), polycarbonate (PC), and polyamide 6 (PA6)—with PE, PP, and PVC being the most common. The total MP levels in bivalves were at ppm levels, ranging from 0.26 µg/g to 36.4 µg/g wet weight. The risk of human ingestion of MPs was assessed through the consumption of bivalves as seafood. The overall potential human health risk value (H) for marine bivalves was classified within the moderate to high hazard category. Full article

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22 pages, 5321 KB

Open AccessArticle

Ecological and Human Health Risks from Potentially Toxic Elements in Environmental Matrices of Kiteezi Landfill, Uganda

by Emmanuel Ebbu, Irene Nalumansi, Ivan Kiganda, Caroline Kiwanuka Nakiguli, Patrick Onen, Simon Ocakacon, Christopher Adaku, Timothy Omara and Emmanuel Ntambi

J. Xenobiot. 2025, 15(6), 185; https://doi.org/10.3390/jox15060185 - 4 Nov 2025

Abstract

By the time of this study, Kiteezi landfill was Uganda’s largest waste disposal site and received substantial volumes of municipal solid waste. In the present study, water (n = 36), leachates (n = 36), superficial sediments (n = 30), and [...] Read more.

By the time of this study, Kiteezi landfill was Uganda’s largest waste disposal site and received substantial volumes of municipal solid waste. In the present study, water (n = 36), leachates (n = 36), superficial sediments (n = 30), and Colocasia esculenta corms (n = 6) were sampled from Kiteezi landfill in the dry and wet seasons of 2022 before its tragic collapse in 2024. The physicochemical parameters (pH, electrical conductivity, temperature, and oxidation–reduction potential) and concentration of potentially toxic elements (As, Cu, Cr, Pb, and Zn) were analyzed using standard methods and inductively coupled plasma-optical emission spectrometry, respectively. Significant seasonal variations (p < 0.05) were observed for all the physicochemical parameters of water and leachates except temperature. Further, significantly higher concentrations (p < 0.05) of potentially toxic elements (PTXEs) were quantified in environmental matrices sampled during the dry season than the wet season. Arsenic and Pb concentrations in water surpassed their WHO permissible limit of 0.01 mg/L. The concentrations of PTXEs were higher in downstream samples (p < 0.05), indicating that landfill activities led to their enrichment in matrices near the facility. Ecological and pollution risk indices indicated that there is severe enrichment of Cu and Zn in the sediments, with dry season downstream samples having contamination factors and geoaccumulation indices of 539.3 and 74.7 and 8.5 and 5.6, respectively. Although ingestion of water may not cause probable health risks, consumption of Colocasia esculenta corms could lead to non-carcinogenic and cancer health risks in both children and adults (hazard indices = 0.085–189.0 and total cancer risk values of 7.33 × 10⁻⁶–4.87 × 10⁻³). These results emphasize the need that any new replacement for Kiteezi landfill should be properly planned and managed to mitigate potential environmental pollution with xenobiotics. Full article

(This article belongs to the Section Ecotoxicology)

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15 pages, 464 KB

Open AccessArticle

Surfactant Protein D Mediates the Association Between Smoking and Type 2 Diabetes Mellitus Incidence in the Spanish Adult Population: Di@bet.es Study

by Wasima Oualla-Bachiri, Ana Lago-Sampedro, Eva García-Escobar, Cristina Maldonado-Araque, Viyey Doulatram-Gamgaram, Marta García-Vivanco, Fernando Martín-Llorente, Juan Luis Garrido, Elías Delgado, Felipe J. Chaves, Luis Castaño, Alfonso Calle-Pascual, Josep Franch-Nadal, Gabriel Olveira, Sergio Valdés and Gemma Rojo-Martínez

J. Xenobiot. 2025, 15(6), 184; https://doi.org/10.3390/jox15060184 - 1 Nov 2025

Abstract

It is well known that environmental factors influence the risk of type 2 diabetes mellitus (T2DM). Several studies have linked the xenobiotics present in tobacco or air pollutants to T2DM development, although the underlying mechanisms remain unclear. Surfactant protein D (SP-D), an immune [...] Read more.

It is well known that environmental factors influence the risk of type 2 diabetes mellitus (T2DM). Several studies have linked the xenobiotics present in tobacco or air pollutants to T2DM development, although the underlying mechanisms remain unclear. Surfactant protein D (SP-D), an immune component released into the bloodstream after lung injury, has been associated with metabolic diseases. The aim of this study was to investigate whether SP-D mediates the effects of smoking or air pollution exposure on T2DM risk in the Spanish adult population. Socio-demographic, lifestyle (including smoking status) and clinical data from 2155 participants from the Di@bet.es cohort were analyzed. Annual concentrations of PM₁₀, PM_2.5, SO₂, CO and NO₂ according to participants’ residential address codes were used to study air pollution exposure. T2DM was diagnosed at baseline and after 7.5 years of follow-up. SP-D serum levels were measured by ELISA and categorized as above or below the 25th percentile. Our results revealed a higher percentage of smokers in the high SP-D category; however, no associations were observed between air pollutants (PM₁₀, PM_2.5, SO₂, CO) and SP-D categories. Both smoking and elevated SP-D levels were found to increase the risk of T2DM independently. Mediation analysis indicated that SP-D mediates 14% of the effect of smoking on T2DM incidence in the Spanish adult population. Full article

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16 pages, 1738 KB

Open AccessArticle

Hematological and Biochemical Alterations Induced by Sub-Acute Administration of Permethrin in Rats

by Liliana Carmona-Aparicio, Elvia Coballase-Urrutia, Marisol Orozco-Ibarra, Norma Serrano-García, Silvia Caballero-Salazar, Maritza Ramírez-Pérez, Liliana Rivera-Espinosa, María E. Hernández, Hortencia Montesinos-Correa, Diana L. Pérez-Lozano and Daniel Diaz

J. Xenobiot. 2025, 15(6), 183; https://doi.org/10.3390/jox15060183 - 1 Nov 2025

Abstract

Permethrin (PERM) is a synthetic pyrethroid insecticide initially regarded as low risk. However, evidence now indicates that misuse and prolonged exposure can damage multiple physiological systems by disrupting enzymatic functions in subcellular structures. In this study, male Wistar rats were administered PERM (75, [...] Read more.

Permethrin (PERM) is a synthetic pyrethroid insecticide initially regarded as low risk. However, evidence now indicates that misuse and prolonged exposure can damage multiple physiological systems by disrupting enzymatic functions in subcellular structures. In this study, male Wistar rats were administered PERM (75, 150, or 300 mg/kg/day) for 15 days to assess its effect on hematological and biochemical parameters, including oxidative stress markers in the liver, kidney, and heart. Subacute PERM administration induced significant, dose-dependent toxicological alterations in exposed animals. Hematological analysis revealed impaired hematopoiesis, characterized by increased erythrocytes and platelets alongside decreased hemoglobin, hematocrit, mean corpuscular volume, and red cell distribution width. Biochemical analysis revealed elevated liver enzymes and bilirubin, along with reduced albumin levels, indicating hepatic alterations associated with PERM. The assessment of oxidative stress revealed tissue-specific responses following PERM exposure. While GPx, CAT, and SOD levels remained unchanged, GR activity increased in the heart, and GST activity increased in the liver. Additionally, a substantial decrease in MDA was observed in both the liver and heart. These collective alterations found in PERM-subacute exposed rats suggest the potential for cellular damage with the possible development of chronic pathologies, warranting further investigation. Full article

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23 pages, 1698 KB

Open AccessArticle

Aqueous Extract of Limnospira platensis Provides Protection Against Microcystin-Induced Oxidative Stress in Hydroponic Culture of Radish (Raphanus sativus)

by Mohammed Haida, Badr Ezzyky, Zineb Hakkoum, Richard Mugani, Yasser Essadki, Fatima El Khalloufi, Abdelmajid Haddioui, Mohamed Loukid, Brahim Oudra and Noureddine Bouaïcha

J. Xenobiot. 2025, 15(6), 182; https://doi.org/10.3390/jox15060182 - 1 Nov 2025

Abstract

The eutrophication of aquatic ecosystems often triggers the excessive growth of cyanobacteria, many of which release toxic metabolites such as microcystins (MCs). When irrigation water is contaminated by these compounds, adverse consequences may arise for plants as well as for animal and human [...] Read more.

The eutrophication of aquatic ecosystems often triggers the excessive growth of cyanobacteria, many of which release toxic metabolites such as microcystins (MCs). When irrigation water is contaminated by these compounds, adverse consequences may arise for plants as well as for animal and human health. In contrast, certain non-toxic cyanobacterial species like Limnospira platensis are increasingly regarded as valuable tools for sustainable agriculture, given their ability to enhance plant nutrition, growth, yield, and stress tolerance while also mitigating the detrimental impacts of MCs. The present work aimed to investigate the potential of L. platensis extract to enhance growth, physiological responses, and tolerance of radish (Raphanus sativus) plants stressed with Microcystis aeruginosa extract containing microcystins. Experiments were conducted in a hydroponic system under controlled environmental conditions, where radish seedlings were cultivated in perlite and exposed for 45 days to M. aeruginosa extract (10 and 40 µg/L of MCs) and L. platensis extract (0.1 and 1 g/L), applied either separately or in combination. The results showed that the application of L. platensis extract, especially at 1 g/L in combination with 40 µg/L of MCs, decreased the bioaccumulation of MCs from 8.81 to 5.35 µg/kg FW in the leaves and from 14.64 to 10.15 µg/kg FW in the taproots. In addition, it significantly stimulated radish growth and improved several biochemical parameters. In contrast, exposure to MCs at 10 and 40 µg/L negatively affected growth, chlorophyll pigments and protein contents while promoting the accumulation of malondialdehyde (MDA), polyphenols and sugars. The activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were also increased under MCs stress, suggesting activation of the antioxidant defense system in response to oxidative damage. Combinations of MCs with L. platensis extract, especially at 1 g/L, improved antioxidant enzyme activities by significantly reducing MDA levels, biometric parameters, chlorophyll pigment, and protein and sugar contents. These results indicate that the application of L. platensis extract as a biostimulant can improve radish development, growth, and tolerance to MC-induced stress. Full article

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17 pages, 1010 KB

Open AccessArticle

Combined Climate and Chemical Stressors: How Spatial Variability Shapes the Response of Ficopomatus enigmaticus (Fauvel, 1923) to Dimethyl Sulfoxide (DMSO) and Heatwaves, and What It Means for Ecotoxicology

by Verdiana Vellani, Manuela Piccardo, Francesca Provenza, Serena Anselmi, Valentina Pitacco, Lovrenc Lipej, Stanislao Bevilacqua and Monia Renzi

J. Xenobiot. 2025, 15(6), 181; https://doi.org/10.3390/jox15060181 - 1 Nov 2025

Abstract

Ficopomatus enigmaticus, a reef-forming serpulid, has emerged as a promising candidate for biomonitoring and ecotoxicology studies. Recent research has focused on adult stress responses, highlighting the need to understand population-specific responses. This study employed a multi-biomarker approach to investigate how F. enigmaticus [...] Read more.

Ficopomatus enigmaticus, a reef-forming serpulid, has emerged as a promising candidate for biomonitoring and ecotoxicology studies. Recent research has focused on adult stress responses, highlighting the need to understand population-specific responses. This study employed a multi-biomarker approach to investigate how F. enigmaticus adults from two populations in the NE Adriatic (Site A) and NE Tyrrhenian (Site B) responded to chronic exposure to heat and chemical stress (dimethyl sulfoxide, DMSO), individually and in combination. The analysis detected significant differences in protein content and the activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) between populations. Notably, no oxidative damage (measured as lipid peroxidation, LPO) was detected in any population or treatment. Similarly, no significant differences were detected in the integrated biomarker response index (IBRv2i). However, lower IBRv2i values at Site A suggested reduced stress conditions, possibly indicating that this site may have lower baseline stress. Overall, treatment effects were limited and site-specific: only the combined heat and DMSO exposure at Site A lowered GST activity compared to heat stress alone. Nevertheless, both populations exhibited broadly similar biochemical response patterns to stress. Our findings deepen the understanding of stress physiology in F. enigmaticus, underscoring the ecological importance of multi-stressor approaches in environmental monitoring. Full article

(This article belongs to the Section Ecotoxicology)

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11 pages, 251 KB

Open AccessArticle

Bisphenol A Levels in Pasteurized Milk Marketed in Plastic Packaging and Associated Health Risk Assessment: A Pilot Study

by El Amine Cheroual, Khatima Mezhoud, Ilaria Neri, Ouahiba Hadjoudj and Lucia Grumetto

J. Xenobiot. 2025, 15(6), 180; https://doi.org/10.3390/jox15060180 - 1 Nov 2025

Abstract

Bisphenol A (BPA) is a synthetic estrogen widely used in the manufacture of food packaging materials, raising concerns due to its potential migration into food products. This study aims to determine BPA levels in pasteurized milk marketed in Algeria, using an easy-to-handle and [...] Read more.

Bisphenol A (BPA) is a synthetic estrogen widely used in the manufacture of food packaging materials, raising concerns due to its potential migration into food products. This study aims to determine BPA levels in pasteurized milk marketed in Algeria, using an easy-to-handle and efficient liquid–liquid extraction method coupled with liquid chromatography and fluorescence detection. A total of 30 pasteurized milk samples packaged in plastic were analyzed. The method validation demonstrated excellent linearity, with a limit of detection of 3.76 µg/L and a limit of quantification of 11.40 µg/L. Among the analyzed samples, 17 contained detectable BPA levels, ranging from not detectable to 24.07 µg/L, with an average concentration of 3.77 ± 5.77 µg/L, compliant with European regulation. The health risk assessment, based on estimated chronic daily intake and hazard index, indicated no significant risk associated with BPA exposure through milk consumption in the studied population. Additionally, the estrogenic equivalence of BPA in milk was 6.032 × 10⁻⁵ µgE₂/L, confirming a low estrogenic activity. Full article

(This article belongs to the Topic Environmental Exposure and Health Risks for Vulnerable Populations)

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24 pages, 1139 KB

Open AccessSystematic Review

Polycyclic Aromatic Hydrocarbons in Honey: A Systematic Review of Occurrence, Concentrations, and Health Risk Assessment

by Wenting Li and Surat Hongsibsong

J. Xenobiot. 2025, 15(6), 179; https://doi.org/10.3390/jox15060179 - 1 Nov 2025

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are toxic pollutants produced by the incomplete combustion of fuels and biomass. They are highly persistent and can accumulate in the food chain. Honey, a natural product susceptible to atmospheric deposition, has recently been recognized as an important bioindicator [...] Read more.

Polycyclic aromatic hydrocarbons (PAHs) are toxic pollutants produced by the incomplete combustion of fuels and biomass. They are highly persistent and can accumulate in the food chain. Honey, a natural product susceptible to atmospheric deposition, has recently been recognized as an important bioindicator for monitoring environmental pollution. This systematic review examined 29 articles published from 2000 to 2025 analyzing the global presence, concentrations, and potential health risks of PAHs in honey. Results showed that the sum of polycyclic aromatic hydrocarbons (ΣPAHs) concentrations in honey ranged from below the detection limit to 166.83 µg/kg. Higher levels were observed in urban and industrial areas. Seventeen studies analyzed 16 PAHs prioritized by the US Environmental Protection Agency (EPA), with benzo[a]pyrene (BaP) being the most frequently detected, a highly toxic compound. Although most samples met international food safety standards, levels exceeding European regulatory limits were detected in some areas, raising concerns about local health risks. The results of this study emphasize the need for standardized analytical methods and routine monitoring to more accurately assess the exposure risk of PAHs in honey. Full article

(This article belongs to the Topic Environmental Exposure and Health Risks for Vulnerable Populations)

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27 pages, 5615 KB

Open AccessArticle

Uncovering Exposure Patterns of Metals, PFAS, Phthalates, and PAHs and Their Combined Effect on Liver Injury Markers

by Doreen Jehu-Appiah and Emmanuel Obeng-Gyasi

J. Xenobiot. 2025, 15(6), 178; https://doi.org/10.3390/jox15060178 - 1 Nov 2025

Abstract

People are exposed to mixtures of metals, per- and polyfluoroalkyl substances (PFAS), phthalates, and polycyclic aromatic hydrocarbons (PAH) rather than single chemicals, yet mixture inference is hampered by high dimensionality, correlation, missingness, and left-censoring below limits of detection (LOD). We analyzed 2013–2014 National [...] Read more.

People are exposed to mixtures of metals, per- and polyfluoroalkyl substances (PFAS), phthalates, and polycyclic aromatic hydrocarbons (PAH) rather than single chemicals, yet mixture inference is hampered by high dimensionality, correlation, missingness, and left-censoring below limits of detection (LOD). We analyzed 2013–2014 National Health and Nutrition Examination Survey (NHANES) biomarkers (n = 4367) to (i) recover latent, interpretable co-exposure structures and (ii) quantify how these mixtures relate to liver health. To denoise and handle censoring, we applied Principal Component Pursuit with LOD adjustment (PCP-LOD), decomposing the exposure matrix into a non-negative low-rank component (population co-exposure profiles) and a sparse component (individual spikes), and then used Bayesian Kernel Machine Regression (BKMR) to estimate nonlinear and interactive associations with AST, ALT, GGT, ALP, total bilirubin, and the Fatty Liver Index (FLI), retaining analytes with ≥50% detection. PCP-LOD revealed coherent clusters (e.g., long-chain PFAS grouping; shared metal loadings), while the sparse layer highlighted episodic phthalate elevations. BKMR indicated outcome-specific mixture effects: PAHs and selected phthalates showed consistently positive associations with ALP, GGT, and FLI; PFAS (PFOS, PFNA, PFOA) exhibited modest associations with ALP and bilirubin; metals displayed mixed directions. A joint increase in the overall mixture from the 25th to 75th percentile corresponded to an upward shift in FLI and a smaller rise in ALT. This censoring-aware low-rank-plus-sparse framework coupled with flexible mixture modeling recovers actionable exposure architecture and reveals clinically relevant links to liver injury and steatosis, motivating longitudinal and mechanistic studies to strengthen causal interpretation. Full article

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14 pages, 1747 KB

Open AccessArticle

Mining Structural Information from Gas Chromatography-Electron-Impact Ionization-Mass Spectrometry Data for Analytical-Descriptor-Based Quantitative Structure–Activity Relationship

by Yasuyuki Zushi

J. Xenobiot. 2025, 15(6), 177; https://doi.org/10.3390/jox15060177 - 1 Nov 2025

Abstract

Recently developed quantitative structure–activity relationship (QSAR) prediction uses machine learning techniques with analytical signals from the full scan of mass spectra as input, and does not need exhaustive structural determination to assess unknown compounds. The QSAR approach assumes that a mass spectral pattern [...] Read more.

Recently developed quantitative structure–activity relationship (QSAR) prediction uses machine learning techniques with analytical signals from the full scan of mass spectra as input, and does not need exhaustive structural determination to assess unknown compounds. The QSAR approach assumes that a mass spectral pattern reflects the structure of a target chemical. However, the relationship between the spectrum and structure is complex, and requirement of its interpretation could restrict further development of QSAR prediction methods based on analytical signals. In this study, whether gas chromatography-electron-impact ionization-mass spectrometry (GC-EI-MS) data contain meaningful structural information that assists QSAR prediction was determined by comparing it with the traditional molecular descriptor used in QSAR prediction. Four molecular descriptors were used: ECFP6, topological descriptor in CDK, MACCS key, and PubChem fingerprint. The predictive performance of QSAR based on analytical and molecular descriptors was evaluated in terms of molecular weight, log K_o-w, boiling point, melting point, water solubility, and two oral toxicities in rats and mice. The influential variables were further investigated by comparing analytical-descriptor-based and linear regression models using simple indicators of the mass spectrum. The investigation indicated that the analytical and molecular descriptors preserved structural information differently. However, their performance was comparable. The analytical-descriptor-based approach predicted the physicochemical properties and toxicities of structurally unknown chemicals, which was beyond the scope of the molecular-descriptor-based approach. The QSAR approach based on analytical signals is valuable for evaluating unknown chemicals in many scenarios. Full article

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15 pages, 746 KB

Open AccessArticle

Influence of Pristine and Photoaging Polystyrene Microspheres on Sperm Quality and DNA Integrity of the Sand Dollars Scaphechinus mirabilis

by Andrey Alexandrovich Mazur, Sergey Petrovich Kukla, Victor Pavlovich Chelomin, Valentina Vladimirovna Slobodskova and Nadezhda Vladimirovna Dovzhenko

J. Xenobiot. 2025, 15(6), 176; https://doi.org/10.3390/jox15060176 - 23 Oct 2025

Abstract

Plastic pollution represents a significant emerging environmental problem. Micro-sized particles of synthetic polymers—microplastics (MPs)—have been identified in all parts of marine ecosystems. In the marine environment, organisms are exposed to MPs, which undergo a constant process of physicochemical and biological degradation. Utilization of [...] Read more.

Plastic pollution represents a significant emerging environmental problem. Micro-sized particles of synthetic polymers—microplastics (MPs)—have been identified in all parts of marine ecosystems. In the marine environment, organisms are exposed to MPs, which undergo a constant process of physicochemical and biological degradation. Utilization of UV irradiation as the optimal exposure factor in the simulation of fundamental natural conditions is a widely accepted approach. This enables the study of the harmful effects of such particles when interacting with aquatic organisms. This study aimed to investigate the effect of pristine and photoaging primary polystyrene microspheres (µPS) at three concentrations on the viability and DNA integrity of the sperm of the sand dollars Scaphechinus mirabilis. The results of the investigation demonstrated that IR spectroscopy revealed structural changes in polystyrene, confirming the oxidative degradation of the polymer under UV irradiation. The study demonstrated that artificially aged µPS exhibited a more pronounced effect than pristine particles, as evidenced by reduced sperm viability and increased DNA damage. Thus, the resazurin test showed that after exposure to UV-irradiated µPS, sperm viability decreased to 83–85% at concentrations of 10 and 100 particles and to 70% at a concentration of 1000. In addition, the Comet assay showed that the particles increased the percentage of DNA in the tail from 20% to 30% in a dose-dependent manner. The findings substantiate and augment the existing body of experimental data of the toxicity of aged plastic fragments, thereby underscoring the need for further study into the toxicity of aged MPs on marine invertebrates. Full article

(This article belongs to the Special Issue (Micro)plastic Pollution: From Monitoring to Toxicity in All Environments)

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15 pages, 1378 KB

Open AccessArticle

Insights into Hepatic, Neuronal, and hERG Channel Safety of Plant-Derived Active Compounds

by Yosita Kasemnitichok, Sion Lee, Oh Bin Kwon, Tullayakorn Plengsuriyakarn and Kesara Na-Bangchang

J. Xenobiot. 2025, 15(6), 175; https://doi.org/10.3390/jox15060175 - 22 Oct 2025

Abstract

Curcumin (CUR), atractylodin (ATD), α-mangostin (αMG), ethyl-p-methoxycinnamate (EPMC), ligustilide (LIG), and β-eudesmol (BEU) are commonly used in Thai traditional medicine formulations. This study evaluated the cytotoxic effects of these compounds in HepG2 liver cancer cells and ReNcell VM neural progenitor cells using the [...] Read more.

Curcumin (CUR), atractylodin (ATD), α-mangostin (αMG), ethyl-p-methoxycinnamate (EPMC), ligustilide (LIG), and β-eudesmol (BEU) are commonly used in Thai traditional medicine formulations. This study evaluated the cytotoxic effects of these compounds in HepG2 liver cancer cells and ReNcell VM neural progenitor cells using the resazurin assay, as well as their potential for hERG inhibition in hERG-overexpressing HEK293 cells, utilizing the automated patch-clamp technique. αMG and CUR significantly reduced HepG2 cell viability (IC₅₀ = 5.5 and 21 µM, respectively). In undifferentiated ReNcell VM cells, αMG was the most potent inhibitor of cell viability (IC₅₀ = 2.1 µM), followed by CUR (IC₅₀ = 21.1 µM), while in differentiated ReNcell VM cells, only αMG exhibited significant neurotoxicity (IC₅₀ = 6.0 µM). Other compounds showed no significant effects on these cells. ATD, BEU, LIG, and EPMC demonstrated low inhibition of hERG channels (IC₅₀ = 26.4, 33.4, 37.3, and 53 µM, respectively), while CUR and αMG displayed weak inhibitory effects (IC50 > 100 µM). αMG may have cytotoxic effects on hepatocytes and neurons at concentrations much higher than when used as medicine or food supplements. At regular clinical doses, αMG, ATD, BEU, EPMC, LIG, and CUR are unlikely to cause significant side effects. However, if these compounds are considered for drug development, their potential effects on hERG channels should be carefully assessed to avoid possible cardiotoxicity. Pharmacokinetics, both preclinical and clinical studies, are necessary to understand the relationship between the plasma concentration profile of EPMC and its potential risks for hepatotoxicity, neurotoxicity, cardiotoxicity, and drug interactions. Full article

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17 pages, 1832 KB

Open AccessArticle

Integrated Monitoring of Water Quality, Metal Ions, and Antibiotic Residues, with Isolation and Optimization of Enrofloxacin-Degrading Bacteria in American Shad (Alosa sapidissima) Aquaculture Systems

by Yao Zheng, Jiajia Li, Ampeire Yona, Xiaofei Wang, Xue Li, Julin Yuan and Gangchun Xu

J. Xenobiot. 2025, 15(6), 174; https://doi.org/10.3390/jox15060174 - 22 Oct 2025

Abstract

This study investigated water quality, metal ion concentrations, and antibiotic residues specifically enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP), across six American shad (Alosa sapidissima) aquaculture sites over a one-year period. Water and sediment samples were analyzed to determine contamination levels, [...] Read more.

This study investigated water quality, metal ion concentrations, and antibiotic residues specifically enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP), across six American shad (Alosa sapidissima) aquaculture sites over a one-year period. Water and sediment samples were analyzed to determine contamination levels, and ENR-degrading bacteria were isolated from the culture environment to explore their potential use in bioremediation. Findings showed that NH₃-N and total suspended solids (TSS) exceeded recommended standards at all sampling sites. Elevated levels of Li, Na (except S1), Fe, Ni (except S2 and S4), Sr, and Cu were found at site S3. Site S5 recorded the highest concentrations of Al, As, and Pb, while Cd was most abundant at S6. In sediments, S5 showed higher levels of Mg, K (except S3), Ca, Cr, Mn, Fe, Ni, As, Pb, Cu, and Zn (except S3). ENR and CIP were detected in all water and sediment samples, with a 100% detection rate. The highest ENR (16.68–3215.95 mg·kg⁻¹) and CIP (3.90–459.60 mg·kg⁻¹) concentrations in water occurred at site S6, following a seasonal pattern of autumn > winter > summer > spring. In sediments, the maximum ENR (41.43–133.67 mg·kg⁻¹) and CIP (12.36–23.71 mg·kg⁻¹) levels were observed in spring. Two ENR-degrading bacterial strains were successfully isolated and identified as Enterococcus and Bacillus. Optimal degradation was achieved at 30 °C, pH 8.0, 6% inoculum, and 3000 Lux, resulting in a 64.2% reduction in ENR after 72 h. Under slightly different conditions (25 °C, pH 10), degradation reached 58.5%. This study provides an efficient strain resource for the bioremediation of ENR pollution in the aquaculture water of American shad. Full article

(This article belongs to the Topic Aquatic Emerging Contaminants and Their Ecotoxicological Consequences, 2nd Edition)

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34 pages, 2365 KB

Open AccessReview

Scientific and Regulatory Perspectives on Chemical Risk Assessment of Pesticides in the European Union

by Fabio Buonsenso

J. Xenobiot. 2025, 15(5), 173; https://doi.org/10.3390/jox15050173 - 21 Oct 2025

Cited by 1

Abstract

People are exposed to pesticides daily through food, drinking water, and the environment, both in urban and rural settings. These chemicals, while offering economic and agricultural benefits through pest control and increased productivity, may pose a growing risk to human health and ecosystem [...] Read more.

People are exposed to pesticides daily through food, drinking water, and the environment, both in urban and rural settings. These chemicals, while offering economic and agricultural benefits through pest control and increased productivity, may pose a growing risk to human health and ecosystem biodiversity. While the European regulatory framework offers a robust foundation for risk assessment, significant limitations persist, especially in addressing cumulative exposure, low-dose effects, and chemical mixtures. This review focuses on selected scientific and regulatory challenges by reviewing recent European Food Safety Authority (EFSA) conclusions, Organization for Economic Co-operation and Development (OECD) test guidelines updates, and current European legislative approaches. Particular attention is given to the regulation of endocrine-disrupting and reprotoxic substances, highlighting progress and remaining gaps in implementation. A brief mention will also be made of immuno-toxic substances, for which no specific hazard class has yet been established. Building on official reports and peer-reviewed literature, this review provides a structured evaluation of the scientific and regulatory landscape, including underexplored issues like the transition to animal-free toxicology and integration of biomonitoring with health data. The goal is to propose realistic, evidence-based improvements to current frameworks using integrated, interdisciplinary approaches that connect toxicology, policy, and implementation science. A shift to a holistic, systems-based, and precautionary paradigm is vital to address emerging challenges and ensure strong protection of health and environment, as well as supporting the needs of the agricultural sector. Full article

(This article belongs to the Special Issue New Challenges in the Monitoring, Risk Assessment and Management of Pesticides and Biocides in the “One Health Era”)

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