Alternative splicing has been shown to participate in tumor progression, including hepatocellular carcinoma. The poor prognosis of patients with HCC calls for molecular classification and biomarker identification to facilitate precision medicine. We...
The development of a sustainable biobased economy (BBE) in Europe is associated with several challenges. Amongst others, lessons learned from the development of the biofuel sector and the complex debate around land use change associated with a growin...
Intracellular ionic strength regulates myriad cellular processes that are fundamental to cellular survival and proliferation, including protein activity, aggregation, phase separation, and cell volume. It could be altered by changes in the activity o...
Bioluminescent reporters and biosensors are widely used to quantify gene expression, evaluate delivery systems and assess biodistribution because they provide low-background readouts over a broad dynamic range. The NanoLuc–furimazine system is...
NanoLuc is a bioluminescent protein recently engineered for applications in molecular imaging and cellular reporter assays. Compared to other bioluminescent proteins used for these applications, like Firefly Luciferase and Renilla Luciferase, it is ~...
Internal ribosomal entry sites (IRESs) are RNA secondary structures that mediate translation independent from the m7G RNA cap. The dicistronic luciferase assay is the most frequently used method to measure IRES-mediated translation. While this assay...
Background/Objectives: Cancer imposes a substantial global public health burden, and its initiation and progression involve complex dysregulation of gene expression regulatory networks. Methods: This study systematically evaluated the expression char...
Background: Although CDK4/6 inhibitors combined with endocrine therapies have improved outcomes in HR+ HER2-negative metastatic breast cancer, predictive biomarkers for treatment response and adverse effects remain limited. This study assessed the pr...
In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated by singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini singlet oxygen gene...
In this paper, we report the very first occurrence of titanian hydroxylclinohumite in the marble-hosted gem spinel deposits of the Luc Yen district, northern Vietnam. Hydroxylclinohumite is anhedral and associated with forsterite, tremolite, pargasit...
Bisdemethoxycurcumin (BDMC) has biological activities, including anticancer effects in vitro; however, its anticancer effects in human glioblastoma (GBM) cells have not been examined yet. This study aimed to evaluate the tumor inhibitory effect and m...
We report a new occurrence of preiswerkite, the rare sodium analog of eastonite, the trioctahedral mica, from marble-hosted noble spinel deposits of the Luc Yen district, northern Vietnam. It is found in marble for the first time. The preiswerkite is...
New city developments (NCDs) have significantly increased around the globe, especially in developing countries, to accommodate population growth and foster economic development. However, the uncertain footprint of NCDs often introduces trade-offs bet...
Recent studies highlight the integral role of the interferon gamma receptor (IFNγR) pathway in T cell–mediated cytotoxicity against solid but not liquid tumors. IFNγ not only directly facilitates tumor cell death by T cells but also...
In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescen...
Some clinically used anti-cancer drugs are obtained from natural products. Allyl isothiocyanate (AITC), a plant-derived compound abundant in cruciferous vegetables, has been shown to possess an anti-cancer ability in human cancer cell lines in vitro,...
Current EU policy calls for decreased emissions of greenhouse gases (GHG) by i.e., replacing fossil fuel in the transportation sector with sustainable biofuels. To avoid indirect land use change (iLUC), the EU at the same time strives to limit the us...
Luigi Russolo’s Intonarumori together with his manifesto L’arte dei rumori (1913) marked a break with the art of clear signification. From here on, noise and dispersed sounds replaced the concept of music reverberating the harmony of...
Tetrandrine (TET), a bisbenzylisoquinoline (BBI) alkaloid, is isolated from the plant Stephania tetrandra S. Moore and has a wide range of biological activity, including anticancer properties in vitro and in vivo. At first, we established a luciferas...
For more than two decades, the phenomenologies of revelation emerging from twentieth century French philosophy have met a North American reception framed largely within the context of a hermeneutic critique. This essay seeks to intervene in this situ...
This essay contends that Jean-Luc Godard’s late digital cinema elaborates a geopolitical aesthetics in which Europe confronts the return of its repressed histories through the very instability of the digital image. While Europe has long functio...
β-sitosterol (SITO) has been reported with anticancer effects; however, with poor bioavailability. The current study aimed to investigate whether liposomal encapsulated β-sitosterol (LS) has a better inhibition effect on tumor metastasis th...
Tooth-bearing structures are commonly involved in the spread of various odontogenic lesions. Radicular and dentigerous cysts are among the most common odontogenic cysts, exhibiting significant variations in size and morphology within the jawbones. On...
This essay examines Jean-Luc Marion’s phenomenal model of the Trinity expounded in his recent book D’Ailleurs, la révélation (2020) and attempts to give an initial assessment from a theological perspective. Since Marion&rsqu...
Murine cytomegalovirus (MCMV), and, in particular, recombinant virus derived from MCMV-bacmid pSM3fr, is widely used as the small animal infection model for human cytomegalovirus (HCMV). We sequenced the complete genomes of MCMV strains and recombina...
This article explores the possibility of conceiving political theology beyond its traditional metaphysical foundations. Starting from Carl Schmitt’s formulation of political theology as a domain rooted in analogical and representational transfe...
Reporter phage assays are a promising alternative to culture-based assays for rapidly detecting viable bacteria. The reporter systems used in phage-based detection are typically enzymes and their corresponding substrates that provide a signal followi...
The role of romantic love in cinema–and its redeeming aspects–has been extensively explored in film studies and beyond. However, non-romantic aspects of love, especially love for the neighbour, have not yet received as much attention. Thi...
Extensive surfaces of land are currently under-utilized, marginal and/or contaminated (MUC) in many EU and neighbouring countries. In the past few years, scientific research has demonstrated that bioenergy crops can potentially render this land profi...
I propose a new reading of a selection of continental philosophical interpretations of the nature of existentialism after the dialogical turn. This analysis will focus on the particular case study of the biblical character of Abraham as a case in poi...
Ca2+-triggered coelenterazine-binding protein (CBP) is a natural form of the luciferase substrate involved in the Renilla bioluminescence reaction. It is a stable complex of coelenterazine and apoprotein that, unlike coelenterazine, is soluble and st...
Multiple land use and land cover (LUC) datasets are available for the analysis of LUC changes (LUCC) in distinct territories. Sometimes, different LUCC results are produced to characterize these changes for the same territory and the same period. The...
Climate and land use/cover (LUC) are the two most significant factors that directly affect the runoff process. However, most research on runoff response has focused mainly on projected climate variation, while future LUC variability has been neglecte...
To govern and ensure the safety of increasing drone operations, the European Aviation Safety Agency (EASA) has defined three risk-based categories of unmanned aircraft system (UAS) operations. A large part of commercial UAS operations fall into the S...
By predicting and analyzing regional land use conflicts (LUCs), the contradictory relationship between urban development and land resources can be revealed, which can assist in achieving the rational use of land resources. Taking Liyang as a case stu...
The use of biofuels to mitigate global carbon emissions is highly dependent on direct and indirect land use changes (LUC). The direct LUC (dLUC) can be accurately evaluated using remote sensing images. In this work we evaluated the dLUC of about 4 mi...
Following general anaesthesia (GA), patients frequently experience sleep disruption and fatigue, which has been hypothesized to result at least in part by GA affecting the circadian clock. Here, we provide the first comprehensive time-dependent analy...
Landslides pose a great threat to the safety of people’s lives and property within disaster areas. In this study, the Zigui to Badong section of the Three Gorges Reservoir is used as the study area, and the land use (LU), land use change (LUC)...
Understanding the processes of land use and land cover changes (LUCC) and the associated driving forces is important for achieving sustainable development. This paper presents the LUCC in Portugal at the regional level (NUTS II) from 1995 to 2010 and...
Two series of benzamides compounds bearing piperidine groups were synthesized and the Gli-luc luciferase activity was screened by Gys-luc luciferase gene detection method. Compound 5q showed promising inhibition of hedgehog (Hh) signaling pathway. To...
New protein-fragment complementation assays (PCA) have successfully been developed to characterize protein–protein interactions in vitro and in vivo. Notably, the NanoBiT technology, employing fragment complementation of NanoLuc luciferase, sta...
Luciferase-based reporters provide a key measurement approach in a broad range of applications, from in vitro high-throughput screening to whole animal imaging. For example, luminescence intensity is widely used to measure promoter activity, protein...
This article proposes a reading of Marion’s phenomenology from an early text, arguing that the various phenomenological innovations which are introduced in this work are subordinated to the central concept of transcendence. This concept in Mari...
Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a “turn-on” bi...
The application of in vivo bioluminescent imaging in infectious disease research has significantly increased over the past years. The detection of transgenic parasites expressing wildtype firefly luciferase is however hampered by a relatively low and...
Whereas Marion’s earlier articulation of givenness has received a wide-spread hermeneutical critic justifiable within the framework of his two major initial texts on the issue (“Reduction and Givenness” and “Being Given”...
Analyzing land use changes (LUC) in both past and future scenarios is critical to optimize local ecology and formulate policies for sustainable development. We analyzed LUC characteristics in Huaibei City, China from 1985 to 2020, and used the CLUE-S...
The debate on the possibility of re-thinking transcendence at the so-called end or closure of the metaphysical tradition and its relation to the political is situated at the heart of contemporary continental philosophy of religion. This article engag...
The land use and land cover (LUC) of the Zêzere watershed (Portugal) have undergone major changes in recent decades, with the increase of artificial surfaces. This trend is quantified in some studies, but the probability of the increase of this type...
To contribute to SDG-15 about the conservation of terrestrial ecosystems, the effective management of land resources is required. In this respect, determining the land use and cover (LUC) based on remote sensing constitutes a strength. For the Arimao...
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