Open AccessArticle
Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique
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Maríafe Laguna 1,2,*, Miguel Holgado 1,2, Ana L. Hernandez 1, Beatriz Santamaría 1,3, Alvaro Lavín 1,2, Javier Soria 4, Tatiana Suarez 4, Carlota Bardina 5, Mónica Jara 5, Francisco J. Sanza 1,3 and Rafael Casquel 1,2
1
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
2
Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2. 28006 Madrid, Spain
3
BioOpticalDetection, Centro de Empresas de la UPM, Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
4
Bioftalmik. Parque Tecnológico Zamudio Ed. 800 2ª Planta 48160, Bizkaia, Spain
5
AntibodyBcn, MRB 104 Modul b UAB Campus, 08193 Bellaterra, Barcelona, Spain
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Abstract
The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1,
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The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.
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