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7,046 Results Found

  • Article
  • Open Access
763 Views
16 Pages

Epichloë Endophyte Alters Bacterial Nitrogen-Cycling Gene Abundance in the Rhizosphere Soil of Perennial Ryegrass

  • Munire Maimaitiyiming,
  • Yanxiang Huang,
  • Letian Jia,
  • Mofan Wu and
  • Zhenjiang Chen

18 July 2025

Perennial ryegrass (Lolium perenne), an important forage and turfgrass species, can establish a mutualistic symbiosis with the fungal endophyte Epichloë festucae var. lolii. Although the physiological and ecological impacts of endophyte infectio...

  • Article
  • Open Access
32 Citations
4,785 Views
17 Pages

Effect of Nitrogen Addition on Soil Microbial Functional Gene Abundance and Community Diversity in Permafrost Peatland

  • Xiuyan Ma,
  • Yanyu Song,
  • Changchun Song,
  • Xianwei Wang,
  • Nannan Wang,
  • Siqi Gao,
  • Xiaofeng Cheng,
  • Zhendi Liu,
  • Jinli Gao and
  • Yu Du

Nitrogen is the limiting nutrient for plant growth in peatland ecosystems. Nitrogen addition significantly affects the plant biomass, diversity and community structure in peatlands. However, the response of belowground microbe to nitrogen addition in...

  • Article
  • Open Access
21 Citations
6,602 Views
15 Pages

PRM1 Gene Expression and Its Protein Abundance in Frozen-Thawed Spermatozoa as Potential Fertility Markers in Breeding Bulls

  • Berlin Pandapotan Pardede,
  • Muhammad Agil,
  • Ni Wayan Kurniani Karja,
  • Cece Sumantri,
  • Iman Supriatna and
  • Bambang Purwantara

3 March 2022

Functional genes and proteins in sperm play an essential role in bulls’ reproductive processes. They are more accurate in determining bull fertility than conventional semen quality tests. Protamine-1 (PRM1) is a gene or protein crucial for pack...

  • Article
  • Open Access
19 Citations
3,438 Views
15 Pages

8 November 2021

Background: Antibiotics releasing from the manufacturing sites to the surrounding environment has been identified as a risk factor for the development of antibiotic resistance of bacterial pathogens. However, the knowledge of the abundance and distri...

  • Article
  • Open Access
2 Citations
3,409 Views
15 Pages

10 October 2023

Rice paddies are dynamic areas for microbe-mediated nitrogen cycling and this could be driven by the long-term alteration of paddy soil edaphic factors. The objective of this study is to evaluate the lasting impact of long-term compost fertilization...

  • Review
  • Open Access
4 Citations
3,292 Views
14 Pages

10 January 2025

Climate change and anthropogenic nitrogen addition alter the soil physicochemical properties and microbial activity in oligotrophic forest soil. Unbalanced and non-selective nitrogen fertilizer application is lost as gas emissions (N2O, NO) and also...

  • Communication
  • Open Access
12 Citations
3,230 Views
13 Pages

Changes in Soil Chemical Properties Due to Long-Term Compost Fertilization Regulate Methane Turnover Related Gene Abundances in Rice Paddy

  • Chungwoo Kim,
  • Denver I. Walitang,
  • Aritra Roy Choudhury,
  • Yi Lee,
  • Sanghun Lee,
  • Hyenchung Chun,
  • Tae-Young Heo,
  • Kido Park and
  • Tongmin Sa

4 March 2022

Maintaining rice yield, soil function, and fertility are essential components of long-term compost fertilization. However, paddy fields are major sources of anthropogenic methane emissions. The aim of the study is to evaluate the changes in soil chem...

  • Article
  • Open Access
9 Citations
4,001 Views
13 Pages

In this study, we sought to profile the abundances and drivers of antibiotic resistance genes in an urban river impacted by combined sewage overflow (CSO) events. Water samples were collected weekly during the summer for two years; then, quantitative...

  • Article
  • Open Access
15 Citations
4,847 Views
18 Pages

Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)

  • Datong Liu,
  • Jing Sun,
  • Dongmei Zhu,
  • Guofeng Lyu,
  • Chunmei Zhang,
  • Jian Liu,
  • Hui Wang,
  • Xiao Zhang and
  • Derong Gao

10 September 2019

Late embryogenesis-abundant (LEA) genes play important roles in plant growth and development, especially the cellular dehydration tolerance during seed maturation. In order to comprehensively understand the roles of LEA family members in wheat, we ca...

  • Article
  • Open Access
15 Citations
6,052 Views
17 Pages

Determination of Butyrate Synthesis Capacity in Gut Microbiota: Quantification of but Gene Abundance by qPCR in Fecal Samples

  • Nikola Daskova,
  • Marie Heczkova,
  • Istvan Modos,
  • Petra Videnska,
  • Petra Splichalova,
  • Helena Pelantova,
  • Marek Kuzma,
  • Jan Gojda and
  • Monika Cahova

2 September 2021

Butyrate is formed in the gut during bacterial fermentation of dietary fiber and is attributed numerous beneficial effects on the host metabolism. We aimed to develop a method for the assessment of functional capacity of gut microbiota butyrate synth...

  • Article
  • Open Access
19 Citations
4,057 Views
19 Pages

Gene Expression and Protein Abundance of Hepatic Drug Metabolizing Enzymes in Liver Pathology

  • Marek Drozdzik,
  • Joanna Lapczuk-Romanska,
  • Christoph Wenzel,
  • Sylwia Szelag-Pieniek,
  • Mariola Post,
  • Łukasz Skalski,
  • Mateusz Kurzawski and
  • Stefan Oswald

Hepatic drug metabolizing enzymes (DMEs) markedly affect drug pharmacokinetics. Because liver diseases may alter enzymatic function and in turn drug handling and clinical efficacy, we investigated DMEs expression in dependence on liver pathology and...

  • Article
  • Open Access
651 Views
21 Pages

Evolution and Reduction in Sulfonamide Resistance Genes in Aerobic Compost of Pig Manure

  • Yihao Huang,
  • Pengyan Wang,
  • Shenao Liu,
  • Shengguo Zhang,
  • Zhuqing Ren and
  • Jian Wu

17 October 2025

This study identified that the absolute abundance of 15 types of antibiotic resistance genes (ARGs) across 21 organic fertilizer samples ranged between 1.15 × 104 and 6.74 × 1010 copies/g, with sulfonamide ARGs and the intI1 gene exhibiti...

  • Article
  • Open Access
7 Citations
3,389 Views
14 Pages

Effects of Three Antibiotics on Nitrogen-Cycling Bacteria in Sediment of Aquaculture Water

  • Zhu Li,
  • Huan He,
  • Jianhe Ding,
  • Zhizhong Zhang,
  • Yifei Leng,
  • Mingjun Liao and
  • Wen Xiong

28 April 2024

Antibiotics are commonly used to prevent and control aquaculture diseases. However, long-term overuse of antibiotics not only leaves residues but also leads to changes in the nitrogen cycle in water, which threatens the survival of aquaculture organi...

  • Article
  • Open Access
8 Citations
3,203 Views
11 Pages

Bacterial Symbionts Confer Thermal Tolerance to Cereal Aphids Rhopalosiphum padi and Sitobion avenae

  • Muhammad Zeeshan Majeed,
  • Samy Sayed,
  • Zhang Bo,
  • Ahmed Raza and
  • Chun-Sen Ma

25 February 2022

High-temperature events are evidenced to exert significant influence on the population performance and thermal biology of insects, such as aphids. However, it is not yet clear whether the bacterial symbionts of insects mediate the thermal tolerance t...

  • Article
  • Open Access
2 Citations
1,693 Views
19 Pages

The Response of Rhizosphere Microbial C and N-Cycling Gene Abundance of Sand-Fixing Shrub to Stand Age Following Desert Restoration

  • Yunfei Li,
  • Bingyao Wang,
  • Zhanjun Wang,
  • Wenqiang He,
  • Yanli Wang,
  • Lichao Liu and
  • Haotian Yang

Rhizosphere microorganisms play a pivotal role in biogeochemical cycles, particularly in relation to carbon (C) and nitrogen (N) cycles. However, the impact of stand age on the composition of rhizosphere microbial communities and the abundance involv...

  • Article
  • Open Access
1 Citations
2,326 Views
14 Pages

14 December 2023

The use of sludge fermentation broth (FB) as a co-metabolic carbon source for treating 2,4,6-trichlorophenol (2,4,6-TCP) wastewater is a novel strategy. The key to the feasibility of this strategy is whether the FB can promote the growth of functiona...

  • Article
  • Open Access
21 Citations
4,193 Views
19 Pages

To investigate the role and microorganism-related mechanisms of macrophytes and assess the feasibility of Oenanthe javanica (Blume) DC. in promoting nitrogen removal in free-water surface constructed wetlands (FWS-CWS) under low temperatures (<10...

  • Article
  • Open Access
6 Citations
3,065 Views
14 Pages

Gene Expression and Protein Abundance of Nuclear Receptors in Human Intestine and Liver: A New Application for Mass Spectrometry-Based Targeted Proteomics

  • Christoph Wenzel,
  • Lisa Gödtke,
  • Anne Reichstein,
  • Markus Keiser,
  • Diana Busch,
  • Marek Drozdzik and
  • Stefan Oswald

20 July 2022

Background: Unwanted drug-drug interactions (DDIs), as caused by the upregulation of clinically relevant drug metabolizing enzymes and transporter proteins in intestine and liver, have the potential to threaten the therapeutic efficacy and safety of...

  • Article
  • Open Access
21 Citations
3,590 Views
13 Pages

Rice Residue-Based Biochar Mitigates N2O Emission from Acid Red Soil

  • Muhammad Aamer,
  • Muhammad Bilal Chattha,
  • Athar Mahmood,
  • Maria Naqve,
  • Muhammad Umair Hassan,
  • Muhammad Shaaban,
  • Fahd Rasul,
  • Maria Batool,
  • Adnan Rasheed and
  • Guoqin Huang
  • + 3 authors

3 December 2021

Biochar application is considered an effective approach to mitigating nitrous oxide (N2O) emissions from agricultural soils. However, the mechanisms of biochar to mitigate N2O emissions from acidic red soils are still unclear. Therefore, the present...

  • Article
  • Open Access
9 Citations
5,147 Views
12 Pages

The limited availability of soil phosphorus to plants under salinity stress is a major constraint for crop production in saline soils, which could be alleviated by improving mycorrhizal and soil microbial interactions. This study investigated the eff...

  • Article
  • Open Access
1 Citations
1,291 Views
20 Pages

Soil Inorganic Phosphorus Is Closely Associated with pqqC- Gene Abundance and Bacterial Community Richness in Grape Orchards with Different Planting Years

  • Xue Wang,
  • Zhubing Shao,
  • Shuo Fang,
  • Jieshan Cheng,
  • Xiaotong Guo,
  • Juan Zhang,
  • Chunyan Yu,
  • Tingting Mao,
  • Guohui Wu and
  • Hongxia Zhang

7 March 2025

The high application rate and low utilization efficiency of inorganic phosphorus (Pi) fertilizer could lead to significant P accumulation in soil. Soil P cycling is greatly affected by the planting time in perennial fruit yards. However, the mechanis...

  • Article
  • Open Access
1,366 Views
19 Pages

Copiapoa solaris is an endemic cactus species from the Antofagasta region, Chile, thriving in arid coastal ecosystems known as “fog oases,” where the rising marine moisture is the primary water source. This study investigates the role of...

  • Article
  • Open Access
5 Citations
3,371 Views
12 Pages

Effects of Dietary Vegetable Oils on Mammary Lipid-Related Genes in Holstein Dairy Cows

  • Einar Vargas-Bello-Pérez,
  • Carolina Geldsetzer-Mendoza,
  • Nathaly Cancino-Padilla,
  • María Sol Morales,
  • Heidi Leskinen,
  • Philip C. Garnsworthy,
  • Juan J. Loor and
  • Jaime Romero

27 December 2019

This study analyzed effects of vegetable oils fed to dairy cows on abundance of genes related to lipid metabolism in milk somatic cells (MSC). During 63 days, 15 cows were allocated to 3 treatments: a control diet with no added lipid the same diet su...

  • Article
  • Open Access
20 Citations
4,880 Views
16 Pages

Specific LTR-Retrotransposons Show Copy Number Variations between Wild and Cultivated Sunflowers

  • Flavia Mascagni,
  • Alberto Vangelisti,
  • Tommaso Giordani,
  • Andrea Cavallini and
  • Lucia Natali

29 August 2018

The relationship between variation of the repetitive component of the genome and domestication in plant species is not fully understood. In previous work, variations in the abundance and proximity to genes of long terminal repeats (LTR)-retrotranspos...

  • Article
  • Open Access
3 Citations
1,851 Views
18 Pages

20 June 2024

The lake littoral zone is periodically exposed to water due to water level fluctuations, driving the succession and distribution of littoral vegetation covers, which complexly affect nutrient biogeochemical transformation. However, the combined effec...

  • Article
  • Open Access
4 Citations
2,470 Views
12 Pages

Effects of Soil Rhizobia Abundance on Interactions between a Vector, Pathogen, and Legume Plant Host

  • Pooja Malhotra,
  • Saumik Basu,
  • Benjamin W. Lee,
  • Liesl Oeller and
  • David W. Crowder

22 February 2024

Soil rhizobia promote nitrogen fixation in legume hosts, maximizing their tolerance to different biotic stressors, plant biomass, crop growth, and yield. While the presence of soil rhizobia is considered beneficial for plants, few studies have assess...

  • Article
  • Open Access
10 Citations
6,754 Views
14 Pages

21 August 2012

Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new ap...

  • Article
  • Open Access
2 Citations
2,204 Views
28 Pages

Mixed forests often increase their stability and species richness in comparison to pure stands. However, a comprehensive understanding of the effects of mixed forests on soil properties, bacterial community diversity, and soil nitrogen cycling remain...

  • Article
  • Open Access
9 Citations
3,149 Views
24 Pages

14 March 2025

This study investigates the effects of different land-use types (forest, arable land, and wetland) on key soil properties, microbial communities, and nitrogen cycling in the Lesser Khingan Mountains. The results revealed that forest (FL) and wetland...

  • Article
  • Open Access
41 Citations
7,732 Views
12 Pages

Low Salivary Amylase Gene (AMY1) Copy Number Is Associated with Obesity and Gut Prevotella Abundance in Mexican Children and Adults

  • Paola León-Mimila,
  • Hugo Villamil-Ramírez,
  • Blanca E. López-Contreras,
  • Sofía Morán-Ramos,
  • Luis R. Macias-Kauffer,
  • Víctor Acuña-Alonzo,
  • Blanca E. Del Río-Navarro,
  • Jorge Salmerón,
  • Rafael Velazquez-Cruz and
  • Samuel Canizales-Quinteros
  • + 2 authors

1 November 2018

Genome-wide association studies (GWAS) have identified copy number variants (CNVs) associated with obesity in chromosomal regions 1p31.1, 10q11.22, 11q11, 16p12.3, and recently 1p21.1, which contains the salivary amylase gene (AMY1). Recent evidence...

  • Article
  • Open Access
1 Citations
1,229 Views
12 Pages

17 February 2025

Bacterial lipopolysaccharides (LPS) have been shown to promote enteric viral infections. This study assessed whether possessing elevated levels of LPS was associated with norovirus infection. Fecal samples from diarrheic norovirus-positive (DNP) (n =...

  • Article
  • Open Access
5 Citations
3,525 Views
19 Pages

The Tatun Volcanic Group (TVG), located in northern Taiwan, is characterized by acidic hot springs where the outflow of the hot springs may affect the properties of the associated lotic water bodies. We investigated the bacterial diversity and functi...

  • Article
  • Open Access
4 Citations
2,059 Views
12 Pages

6 June 2024

This study aimed to assess the influences of short-term straw return combined with nitrogen (N) fertilizer on crop yield, soil properties, the bacterial community, and soil nitrogen cycling gene abundance in a rice–rapeseed planting system. A t...

  • Article
  • Open Access
3 Citations
3,763 Views
15 Pages

Intracellular Localization and Gene Expression Analysis Provides New Insights on LEA Proteins’ Diversity in Anhydrobiotic Cell Line

  • Sabina A. Kondratyeva,
  • Taisiya A. Voronina,
  • Alexander A. Nesmelov,
  • Yugo Miyata,
  • Shoko Tokumoto,
  • Richard Cornette,
  • Maria V. Vorontsova,
  • Takahiro Kikawada,
  • Oleg A. Gusev and
  • Elena I. Shagimardanova

22 March 2022

Anhydrobiosis, an adaptive ability to withstand complete desiccation, in the nonbiting midge Polypedilum vanderplanki, is associated with the emergence of new multimember gene families, including a group of 27 genes of late embryogenesis abundant (LE...

  • Article
  • Open Access
15 Citations
5,266 Views
14 Pages

Linking Soil Microbial Properties with Plant Performance in Acidic Tropical Soil Amended with Biochar

  • Muhammad Farid Azlan Halmi,
  • Siti Nahdiyyah Hasenan,
  • Khanom Simarani and
  • Rosazlin Abdullah

8 November 2018

Soil microbial properties are frequently used as indicators of soil fertility. However, the linkage of these properties with crop biomass is poorly documented especially in biochar amended soil with high carbon:nitrogen (C:N). A short-term field tria...

  • Feature Paper
  • Article
  • Open Access
1 Citations
2,146 Views
7 Pages

27 November 2021

We investigated the extent of the heritability underestimation for molecules from an infinitesimal model in mixed model analysis. To this end, we estimated the heritability of transcription, ribosome occupancy, and translation in lymphoblastoid cell...

  • Article
  • Open Access
3 Citations
2,607 Views
14 Pages

Sediment particle size and heterogeneity play an important role in sediment denitrification through direct and indirect effects on, for example, the material exchange rate, environmental gradients, microbial biomass, and grazing pressure. However, th...

  • Article
  • Open Access
13 Citations
6,009 Views
21 Pages

Use of Metagenomic Whole Genome Shotgun Sequencing Data in Taxonomic Assignment of Dipterygium glaucum Rhizosphere and Surrounding Bulk Soil Microbiomes, and Their Response to Watering

  • Ashwag Shami,
  • Rewaa S. Jalal,
  • Ruba A. Ashy,
  • Haneen W. Abuauf,
  • Lina Baz,
  • Mohammed Y. Refai,
  • Aminah A. Barqawi,
  • Hanadi M. Baeissa,
  • Manal A. Tashkandi and
  • Aala A. Abulfaraj
  • + 1 author

18 July 2022

The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of the rhizosphere microbiomes of Dipterygium glaucum and surrounding bulk soil microbiomes, and to detect differential microbial responses due to watering....

  • Article
  • Open Access
9 Citations
3,596 Views
19 Pages

25 June 2021

Peripheral blood transcriptome is a highly promising area for biomarker development. However, transcript abundances (TA) in these cell mixture samples are confounded by proportions of the component leukocyte subpopulations. This poses a challenge to...

  • Article
  • Open Access
11 Citations
5,682 Views
16 Pages

Decoding the Divergent Subcellular Location of Two Highly Similar Paralogous LEA Proteins

  • Marie-Hélène Avelange-Macherel,
  • Adrien Candat,
  • Martine Neveu,
  • Dimitri Tolleter and
  • David Macherel

Many mitochondrial proteins are synthesized as precursors in the cytosol with an N-terminal mitochondrial targeting sequence (MTS) which is cleaved off upon import. Although much is known about import mechanisms and MTS structural features, the varia...

  • Review
  • Open Access
26 Citations
5,878 Views
15 Pages

Workability of mRNA Sequencing for Predicting Protein Abundance

  • Elena A. Ponomarenko,
  • George S. Krasnov,
  • Olga I. Kiseleva,
  • Polina A. Kryukova,
  • Viktoriia A. Arzumanian,
  • Georgii V. Dolgalev,
  • Ekaterina V. Ilgisonis,
  • Andrey V. Lisitsa and
  • Ekaterina V. Poverennaya

11 November 2023

Transcriptomics methods (RNA-Seq, PCR) today are more routine and reproducible than proteomics methods, i.e., both mass spectrometry and immunochemical analysis. For this reason, most scientific studies are limited to assessing the level of mRNA cont...

  • Article
  • Open Access
2 Citations
1,221 Views
17 Pages

20 December 2024

Agricultural soils are major anthropogenic sources of N2O emissions. The application of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) has been proved to be an effective management measure to mitigate N2O emissions. However, the influe...

  • Article
  • Open Access
895 Views
18 Pages

4 September 2025

Climate change and injudicious nitrogen addition alter the soil physico-chemical properties and microbial activity in oligotrophic forest soil, which disrupts the nitrogen cycle balance. Nevertheless, recommended fertilizer forms and levels are consi...

  • Article
  • Open Access
12 Citations
4,314 Views
15 Pages

In Nitrate-Rich Soil, Fallopia x bohemica Modifies Functioning of N Cycle Compared to Native Monocultures

  • Amélie A. M. Cantarel,
  • Soraya Rouifed,
  • Laurent Simon,
  • Julien Bourg,
  • Jonathan Gervaix,
  • Leslie Blazère,
  • Sophie Poussineau,
  • Charline Creuzé des Châtelliers and
  • Florence Piola

17 April 2020

The effects of invasive species at the ecosystem level remain an important component required to assess their impacts. Here, we conducted an experimental study with labeled nitrogen in two types of soil (low and high nitrate conditions), investigatin...

  • Article
  • Open Access
40 Citations
5,023 Views
19 Pages

The Effects of Environmental Factors on Ginsenoside Biosynthetic Enzyme Gene Expression and Saponin Abundance

  • Tao Zhang,
  • Mei Han,
  • Limin Yang,
  • Zhongming Han,
  • Lin Cheng,
  • Zhuo Sun and
  • Linlin Yang

20 December 2018

Panax ginseng C.A. Meyer is one of the most important medicinal plants in Northeast China, and ginsenosides are the main active ingredients found in medicinal ginseng. The biosynthesis of ginsenosides is regulated by environmental factors and the exp...

  • Article
  • Open Access
4 Citations
3,050 Views
18 Pages

8 September 2022

The roles of bacterial communities in the health of soil microenvironments can be more adequately defined through longer-term soil management options. Carbon dioxide (CO2) fixation by autotrophic bacteria is a principal factor in soil carbon cycles....

  • Article
  • Open Access
63 Citations
6,437 Views
15 Pages

5 September 2019

Lipoxygenases (LOXs; EC 1.13.11.12) catalyze the oxygenation of fatty acids to produce oxylipins including the jasmonate family of plant hormones. The involvement of jasmonates in plant growth and development and during abiotic stress has been docume...

  • Article
  • Open Access
20 Citations
6,657 Views
22 Pages

25 February 2022

Soil moisture is the major factor influencing microbial properties and nitrous oxide (N2O) production. Agricultural soils can be probed under wetting, wet/dry alternating, and constant moisture conditions to evaluate the combined effects of early (pr...

  • Article
  • Open Access
6 Citations
4,720 Views
22 Pages

Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen

  • Sungmin Hwang,
  • Nikita E. Chavarria,
  • Rylee K. Hackley,
  • Amy K. Schmid and
  • Julie A. Maupin-Furlow

26 September 2019

Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. vo...

  • Article
  • Open Access
6 Citations
8,669 Views
10 Pages

Myosin Gene Expression and Protein Abundance in Different Castes of the Formosan Subterranean Termite (Coptotermes formosanus)

  • Matthew R. Tarver,
  • Christopher B. Florane,
  • Christopher P. Mattison,
  • Beth A. Holloway and
  • Alan Lax

16 November 2012

The Formosan subterranean termite (Coptotermes formosanus) is an important worldwide pest, each year causing millions of dollars in structural damage and control costs. Termite colonies are composed of several phenotypically distinct castes. Termites...

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