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Molecules 2019, 24(1), 14; https://doi.org/10.3390/molecules24010014

The Effects of Environmental Factors on Ginsenoside Biosynthetic Enzyme Gene Expression and Saponin Abundance

Co-constructing Key Laboratory by Province and the Ministry of Science and Technology of Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China
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Academic Editor: Jose M. Palomo
Received: 23 November 2018 / Revised: 14 December 2018 / Accepted: 17 December 2018 / Published: 20 December 2018
(This article belongs to the Special Issue Biomolecular Catalysts)
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Abstract

Panax ginseng C.A. Meyer is one of the most important medicinal plants in Northeast China, and ginsenosides are the main active ingredients found in medicinal ginseng. The biosynthesis of ginsenosides is regulated by environmental factors and the expression of key enzyme genes. Therefore, in this experiment, ginseng in the leaf opened stage, the green fruit stage, the red fruit stage, and the root growth stage was used as the test material, and nine individual ginsenosides and total saponins (the sum of the individual saponins) were detected by HPLC (High Performance Liquid Chromatography). There was a trend of synergistic increase and decrease, and saponin accumulation and transfer in different tissues. The expression of key enzyme genes in nine synthetic pathways was detected by real-time PCR, and the correlation between saponin content, gene expression, and ecological factors was analyzed. Correlation analysis showed that in root tissue, PAR (Photosynthetically Active Radiation) and soil water potential had a greater impact on ginsenoside accumulation, while in leaf tissue, temperature and relative humidity had a greater impact on ginsenoside accumulation. The results provide a theoretical basis for elucidating the relationship between ecological factors and genetic factors and their impact on the quality of medicinal materials. The results also have guiding significance for realizing the quality of medicinal materials. View Full-Text
Keywords: Panax Ginseng; gene expression; saponin; environmental factors; HPLC Panax Ginseng; gene expression; saponin; environmental factors; HPLC
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Zhang, T.; Han, M.; Yang, L.; Han, Z.; Cheng, L.; Sun, Z.; Yang, L. The Effects of Environmental Factors on Ginsenoside Biosynthetic Enzyme Gene Expression and Saponin Abundance. Molecules 2019, 24, 14.

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