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Open AccessArticle

Effects of Dietary Vegetable Oils on Mammary Lipid-Related Genes in Holstein Dairy Cows

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Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Casilla 306, Santiago 6904411, Chile
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Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, DK-1870 Frederiksberg C, Denmark
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Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa, La Pintana, Santiago 11735, Chile
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Milk Production, Production Systems, Natural Resources Institute Finland (Luke), FI-31600 Jokioinen, Finland
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School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
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Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA
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Laboratorio de Biotecnología en Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Avda. El Libano 5524, Macul, Santiago 7830490, Chile
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Author to whom correspondence should be addressed.
Animals 2020, 10(1), 57; https://doi.org/10.3390/ani10010057
Received: 17 October 2019 / Revised: 22 December 2019 / Accepted: 23 December 2019 / Published: 27 December 2019
(This article belongs to the Section Animal Nutrition)
This study analyzed effects of vegetable oils fed to dairy cows on abundance of genes related to lipid metabolism in milk somatic cells (MSC). During 63 days (9 weeks), 15 cows were allocated to 3 treatments: a control diet with no added lipid and the same diet supplemented with olive oil (OO, 30 g/kg DM) or hydrogenated vegetable oil (HVO, 30 g/kg DM). Dietary oil supplementation (3% DM) had a modest nutrigenomic effect on biological functions such as acetate and FA activation and intra-cellular transport, lipid droplet formation, and transcription regulation in MSC. Results suggest that long-term dietary monounsaturated and saturated lipids could alter mRNA abundance in MSC from mid-lactating cows.
This study analyzed effects of vegetable oils fed to dairy cows on abundance of genes related to lipid metabolism in milk somatic cells (MSC). During 63 days, 15 cows were allocated to 3 treatments: a control diet with no added lipid the same diet supplemented with olive oil (OO, 30 g/kg DM) or hydrogenated vegetable oil (HVO, 30 g/kg DM). On days 21, 42 and 63, MSC were obtained from all cows. Relative abundance of genes involved in lipid metabolism in MSC from cows fed control on days 42 and 63 was compared with relative abundance at day 21 to evaluate fold-changes. Those genes without changes over the time were selected to analyze effects of OO and HVO. Compared with control, on day 42, PLIN2 and THRSP were upregulated by OO. Compared with control, on day 21, HVO up regulated ACACA, down regulated FABP3, and on day 63 THRSP and FABP4 were down regulated. Dietary oil supplementation (3% DM) had a modest nutrigenomic effect on different biological functions such as acetate and FA activation and intra-cellular transport, lipid droplet formation, and transcription regulation in MSC. View Full-Text
Keywords: gene abundance; mammary gland; milk somatic cells; transcriptomic; olive oil gene abundance; mammary gland; milk somatic cells; transcriptomic; olive oil
MDPI and ACS Style

Vargas-Bello-Pérez, E.; Geldsetzer-Mendoza, C.; Cancino-Padilla, N.; Morales, M.S.; Leskinen, H.; Garnsworthy, P.C.; Loor, J.J.; Romero, J. Effects of Dietary Vegetable Oils on Mammary Lipid-Related Genes in Holstein Dairy Cows. Animals 2020, 10, 57.

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  • Supplementary File 1:

    PDF-Document (PDF, 271 KB)

  • Externally hosted supplementary file 1
    Doi: 10.5281/zenodo.3497179
    Link: https://zenodo.org/record/3497179#.XahThegzZaQ
    Description: Table S1. qPCR performance of target genes and internal controls (GAPDH, EIF3K and UXT) Table S2. Relative expression of genes involved in lipid metabolism in milk somatic cells from cows fed control (no fat supplementation) on days 42 and 63 using the relative abundance of day 21 as a reference condition Table S3. Relative abundance of genes involved in lipid metabolism in milk somatic cells from cows fed with olive oil (OO) on days 42 and 63 using the relative abundance of day 21 as a reference condition Table S4. Relative abundance of genes involved in lipid metabolism in milk somatic cells from cows fed with hydrogenated vegetable oil (HVO) on days 42 and 63 using the relative abundance of day 21 as a reference condition
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