Decalcification of hard tissues such as bone and teeth is a complex process that requires using chemicals such as acids and chelating agents. Acids act faster than chelating agents, but they have a greater risk of damaging biological samples. Increas...
Background: RT-qPCR is the gold standard for quantitative gene expression analysis, but it requires homogenized tissue and thus loses spatial information. RNA in situ hybridization (ISH) preserves tissue localization but is technically challenging, e...
Histological techniques are essential for studying small crustaceans’, such as Neocaridina shrimp, anatomy and physiology. However, their small size and rapid tissue autolysis present challenges for fixation and processing. This study aimed to...
Myo-inositol hexaphosphate (phytate; IP6) is a natural compound that is abundant in cereals, legumes, and nuts, and it can bind to crystal surfaces and disturb crystal development, acting as crystallization inhibitor. The adsorption of such inhibitor...
Cetaceans greatly depend on their hearing system to perform many vital activities. The spiral ganglion is an essential component of the auditory pathway and can even be associated with injuries caused by anthropogenic noise. However, its anatomical l...
(1) Background: Osteoarthritis is a degenerative disease of the whole joint marked by cartilage–bone interface (CBI) remodeling, but methods to monitor subtle changes in mineralization are lacking. We optimized a non-destructive ultrasound imag...
Background: Bone tissue decalcification is essential for histopathological evaluation, but conventional methods using inorganic acids degrade nucleic acids, limiting molecular testing. EDTA is known to better preserve DNA, but its suitability for nex...
The most often dispersed environmental pollutants that are released both directly and indirectly into the environment that may eventually reach aquatic ecosystems and contaminate aquatic biomes are cigarette butts (CBs). Toxicants such as nicotine, d...
This paper investigates the synergistic interaction between carbonation and microcracks in hardened cement pastes. Ordinary Portland cement pastes with three different water/cement ratios of 0.4, 0.5 and 0.6, hydrated for 28 days with crack apertures...
The production of urea, used inter alia in agriculture, is increasing. Therefore, urea hydrolysis products are expected in groundwater. Due to lack of new research on the influence of urea on the technical condition of concrete structures, the change...
The demineralization process conditions the structure of the enamel and begins with a superficial decalcification procedure that makes the enamel surface porous and gives it a chalky appearance. White spot lesions (WSLs) are the first clinical sign t...
The strategic idea in this work was to increase pH values by employing natural alkali sources (i.e., HCO3− and CO32−) from four tested agro-ashes as an alternative to chemicals (i.e., lime or soda ash). The considerable proportion of carbonates and b...
Human survival is threatened by the rapid climate change due to global warming caused by the increase in CO2 emissions since the Second Industrial Revolution. This study developed a secondary cement product production technology by replacing cement,...
During orthodontic treatment, the early diagnosis of microscopic changes in soft and hard tissues, including periodontal tissue, is very important to prevent iatrogenic side effects like root resorption and periodontal diseases. Cervical periodontal...
The article analyzes the composition of concrete taken from various elements from a 100-year-old bridge in South Poland, so as to analyze its technical condition. The main methods applied during experimental work were: Designation of pH, free chlorid...
Distraction osteogenesis is a clinically established technique for lengthening, molding and shaping bone by new bone formation. The experimental evaluation of this expensive and time-consuming treatment is of high impact for better understanding of t...
To construct a novel artificial cornea biomaterial, a method to prepare collagen lamellar matrix was developed in this study using grass carp scales as raw materials. The relationship between the structure of fish scale collagen lamellar matrix and t...
The phase changes in alkali-activated slag samples when exposed to supercritical carbonation were evaluated. Ground granulated blast furnace slag was activated with five different activators. The NaOH, Na2SiO3, CaO, Na2SO4, and MgO were used as activ...
Aortic valve stenosis has become the most common valvular disease in elderly patients. Several treatments are available such as surgical aortic valve replacement and transcatheter aortic valve implantation. To date, however, there is a need to discov...
This study presents findings on the biological action of an integrated supplement containing the following components involved in osteogenesis and mineralization: vitamin D and silicon in the bioavailable and soluble form. A hypothesis that these com...
This study presents an analytical and multiscale investigation of the degradation of elastic properties in ordinary Portland cement (OPC) paste subjected to calcium leaching. Eight representative microstructures and three homogenization schemes (Mori...
The durability of C3S-C3A paste with varied C3A content (0%, 5%, 10%, and 20%) against sulfate attack at various attack ages (3 d, 7 d, 28 d, and 180 d) was investigated in this study through the examinations of corrosion product composition, Ca/Si a...
We report on the worldwide first implantation of a transcatheter valve in mitral position using a surgical approach to treat severe combined mitral valve disease, complicated by massive calcifications of the whole mitral anulus. The management of suc...
TRPC channels are critical players in cochlear hair cells and sensory neurons, as demonstrated in animal experiments. However, evidence for TRPC expression in the human cochlea is still lacking. This reflects the logistic and practical difficulties i...
The degree of ossification of the thyroid cartilage in anthropological studies is related to other methods of determining the age of a skeleton. The endochondral ossification process begins at the age of approximately 15–20 years and is general...
Introduction: The most commonly encountered foreign body in the vagina is a tampon, which is routinely removed during gynaecological examinations. While these cases are common and well-known in medical practice, there are also situations involving mu...
Iontophoresis is a noninvasive method to enhance systemic and local drug delivery by the application of an electric field. For systemic drug delivery in the oral cavity, iontophoresis was studied primarily for transbuccal delivery. Significant enhanc...
Application of next-generation sequencing (NGS) technology to routine clinical practice has enabled characterization of personalized cancer genomes to identify patients likely to have a response to targeted therapy. The proper selection of tumor samp...
The geologic disposal of radioactive waste could lead to confined conditions in which cementitious materials impose a moderate alkaline pH, in which the rocks supply sulfate ions at rather low concentrations. In this context, the purpose of this work...
In recent years, an increase in children diagnosed with molar incisor hypomineralization (MIH) has been observed. Children with MIH show a high failure rate with conservative treatment. The ICON® system (DMG, Hamburg, Germany), which is an infilt...
To investigate the effect of different CO2 concentrations on the carbonation results of slag blended cement pastes, carbonation experiments under natural (0.03% CO2) and accelerated conditions (3, 20, and 100% CO2) were investigated with various micr...
The paper assesses the durability of one-part alkali-activated slag-based mortars (AAS) in different aggressive environments, such as calcium chloride- and magnesium sulphate-rich solutions, in comparison with traditional cementitious mortars at equa...
The nacreous layer of shells and pearls is composed of aragonite crystals arranged in an organic matrix. The organic matrix contains chitin and several proteins that regulate the formation of the nacreous layer. Owing to their strong interactions in...
In this paper, we comparitvley studied acetic acid attacks on geopolymer (GP-M), calcium aluminate (CAC-M), and Portland cement (PC-M)-based mortars. Consequent formations of deteriorated or transition layers surrounding the unaltered core material w...
A new range of grouts prepared by air lime and metakaolin (MK) as a pozzolanic admixture has been obtained by using as dispersing agents two polymers, namely poly-naphthalene sulfonate (PNS) and lignosulfonate (LS), with the aim of improving the flui...
17 December 2020
The present study aimed to evaluate the effect of the oral-hygiene regime on the incidence of enamel demineralization in young patients treated with fixed appliances. Research was conducted in a group of 144 patients aged 12–18 years, divided i...
To develop a new method for extracting gelatin from the sturgeon head, the conditions for pretreatment and extraction were optimized. Treatment at 65 °C (3–3.5 h) was enough to separate the head into mixed tissues (skin, scales, pectoral fi...
(1) Background: The tissue engineering field has been working to find biomaterials that mimic the biological properties of autogenous bone grafts. (2) Aim: To evaluate the osteoconduction potential of injectable calcium phosphate cement implanted in...
Ionic calcium can help in the prevention of the process of osseous decalcification. This study aimed to evaluate the physicochemical properties and toxic effects of ionic calcium-fiber supplement (ICa+) and its impact on bone health preservation in m...
We studied changes in articular calcified cartilage (ACC) and subchondral bone (SCB) in the third carpal bones (C3) of Standardbred racehorses with naturally-occurring repetitive loading-induced osteoarthritis (OA). Two osteochondral cores were harve...
The worldwide pursuit of new eco-efficient pozzolans is ongoing. Kaolinite-based waste is an eco-friendly source of recycled metakaolinite, a highly pozzolanic product. In this study, a blended cement paste containing 20% activated coal waste (ACW) w...
Cementitious materials have potential for infrastructure development in low-temperature marine environments, including in seawater at high latitudes and in deep-sea environments (water depths of >1000 m). Although the marine deterioration of cemen...
Concrete structures have to withstand the combined effects of external load and environmental factors. Therefore, it is meaningful to study the durability of concrete under compression and carbonation. The air permeability coefficient (kAu) and pore...
To validate the reliability and implementation of an objective diagnostic method for giant cell tumour of bone (GCTB). H3-3A gene mutation testing was performed using two different methods, Sanger sequencing and immunohistochemical (IHC) assays. A to...
This experiment investigated the mechanical performance and acid resistance (when subjected to 28 days of exposure to sulfuric and nitric acid of five percent) of ambient-cured geopolymer concrete. Geopolymer concrete (GPC)—which is produced by...
Concretes can be exposed to a magnesium attack in several environments leading to the formation of magnesium silicate hydrates (M-S-H) and brucite (MH). The formation of M-S-H is likely to alter the properties of the cement matrix because it is linke...
There are treatments available for enamel demineralization or acid erosion, but they have limitations. We aimed to manufacture a device that could directly form a hydroxyapatite (HAp) film coating on the enamel with a chairside erbium-doped yttrium a...
This study investigates the long-term durability and crystallization-induced degradation mechanisms of alkali-activated slag (AAS) mortars with varying water-to-binder ratios (w/b, 0.4, 0.45, 0.5) under semi-immersion in 5 wt.% sodium sulfate solutio...
The durability of construction and building materials under sulphate environments is an important indicator to evaluate their service life. In this study, the physical and mechanical behaviours of wood-ash-based alkali-activated materials (AAMs) inco...
This study involves the preparation of scale collagen peptides (SCPs) with whitening activity from silver carp (Hypophthalmichthys molitrix) and their characterization and peptide sequence identification. In this article, scanning electron microscopy...
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