Forensic Sciences

Background: Estimating the postmortem interval (PMI) is complicated by extrinsic environmental and intrinsic individual factors. Methods: Improved accuracy may be achieved through a better understanding of desiccation. This study examines moisture loss and desiccation in human remains in western North Carolina, validating previous research in central Texas. Ten donated individuals were placed across three seasonal trials at Western Carolina University’s Forensic Osteology Research Station (FOREST). Soft tissue moisture measurements were recorded from 20 locations on the body using a Delmhorst RDM-3^TM meter, and environmental data were recorded on-site. Results: Moisture content declined rapidly until ~500 accumulated degree days (ADD), after which patterns became highly variable. Linear mixed-effects models identified temperature as the strongest predictor of moisture loss, particularly in spring and fall, while precipitation was the most influential in summer, coinciding with rapid skeletonization. Compared to central Texas, western North Carolina exhibited less consistent moisture loss patterns and greater environmental variability. Fixed effects explained 36–63% of moisture variation across body regions, with conditional R² values modestly higher when accounting for individual differences. Conclusions: These findings highlight the importance of region-specific research for PMI estimation. Full article

Background: The Tawahka ethnic group, with approximately 2690 individuals in northeastern Honduras, represents one of the country’s smallest indigenous communities. No genetic studies have been published on this population, and population-specific databases are essential for forensic applications. Methods: Allele frequencies for 23 autosomal short tandem repeats (STRs) loci were analyzed in 100 unrelated Tawahka individuals (61 females, 39 males) from the municipality of Wampusirpi. Deoxyribonucleic acid (DNA) was extracted from blood on Fast Technology for Analysis of nucleic acids (FTA) cards and amplified using the PowerPlex Fusion 6C System. Statistical parameters were calculated using Genepop v4.2 and Arlequin v5.3.2.2. Results: All loci showed substantial polymorphism with no Hardy–Weinberg equilibrium deviations after Bonferroni correction (α = 0.0022). Expected heterozygosity ranged from 0.4968 to 0.8812. Combined power of discrimination was 99.9999% and combined chance of exclusion was 99.99%. Conclusions: This first genetic characterization of the Tawahka population provides essential reference data for forensic identification, paternity testing, and population genetics studies. The dataset contributes to understanding indigenous Central American genetic diversity and ensures accurate forensic analyses for individuals of Tawahka ancestry following Combined DNA Index System (CODIS) and European Standard Set (ESS) standards. Full article

Background/Objectives: Forensic anthropological analyses and reports include diverse information that may help in establishing identifications, cause or manner of death determinations, taphonomic alterations, or other circumstances involving decedents. However, within forensic anthropology in the United States, there remains no unifying standardization for analyzing cases or writing reports that can be used independent of work environment, education, or case circumstance, which is potentially problematic for a field with 32.4% of practitioners being sole practitioners, as reported here. While some broad “best practice” guidelines exist, they do not provide a significant, nuanced, or unifying set of methodological, terminology, or reporting standards. Methods: In order to explore whether a lack of standardization is problematic in forensic anthropology, a survey was conducted to explore reporting practices, followed standards, and personal experiences regarding overall practices that lead to case reports. Results: Among the 106 respondents, there were multiple areas of agreement, which suggests that unofficial reporting standardization exists within the field. Further, 71.3% agreed that standardization in case reporting is important, and 60.3% supported the adoption of universal standards. However, other areas, such as cognitive bias, terminology, education, and training experiences, varied among respondents. Standardization is important, as 23.2% reported that their laboratory lacks a Standard Operating Procedure; 28.0% reported that their laboratory lacks reporting guidelines; and 24.4% reported that their casework was not peer reviewed. Discussion/Conclusions: The findings from this study suggest that overarching standardization for report writing, terminology, and methodology should be created to provide a baseline reference for all forensic anthropologists, regardless of work context and educational background. Full article

Background: Fatal hemorrhage from ruptured varicose veins is a rare event that may mimic violent death given the quantity and distribution of blood at the scene. Accurate determination of cause and manner of death requires careful scene investigation, autopsy, histology, and ancillary methods such as bloodstain pattern analysis (BPA). Case presentation: We describe an 80-year-old woman found deceased at home, with evidence of extensive bloodstaining, no significant external trauma except a small laceration over a varicosity, and ultimately a ruptured branch of the small saphenous vein. The cause of death was hemorrhagic shock due to spontaneous varicose vein rupture. Discussion: This case reinforces the importance of integrating death scene findings (including shoe prints, bloodstain distribution), anatomical dissection, histology, and toxicology to exclude non-natural causes. We also review recent similar cases in the literature, noting risk factors, presentation, and common pitfalls. Conclusions: Forensic investigations of massive bleeding in elderly, isolated individuals should include varicose vein rupture in the differential. A multidisciplinary approach is essential to avoid misclassification of manner of death. Full article

Background: Sex estimation from skeletal remains is important for forensic identification, but many methodologies focus on complete elements despite high fragmentation rates in operational contexts. The aim of this study was to develop and validate discriminant function equations for sex estimation between complete and fragmented Thai femora. Materials and Methods: A total of 560 adult femora (280 males and 280 females) were used for measurements of eight osteometric variables. Then, discriminant function analysis was applied to complete femora and anatomically isolated segments, including proximal, diaphyseal, and distal, with leave-one-out cross-validation. Results: All measurements showed significant sexual dimorphism, with percentage differences ranging from 6.56% to 42.27%. Complete femur stepwise analysis achieved 90.47% accuracy by using four optimally selected variables, performing comparably to eight-variable models. Isolated segment accuracies varied substantially: proximal segments achieved 89.64% accuracy, differing by only 0.83 percentage points from complete performance; distal segments demonstrated 86.25% accuracy from bicondylar width alone; and diaphyseal segments achieved 80.88%. Combined segment approaches demonstrated synergistic effects approaching complete femur performance. Conclusions: These population-specific equations provided validated methodologies for sex estimation from complete and fragmentary Thai femora. Anatomical region selection still maintained high classification accuracy despite skeletal incompleteness, in line with the fragmentary conditions commonly encountered in forensic and archeological contexts. Full article

Background/Objectives: Motorcycle accidents have grown to become a significant public health thread worldwide. Most of the victims are described to be males of young age, some of lower socioeconomic status. Throughout the literature, head and spinal injuries are described as the most common injuries, while chest injuries and lower extremity fractures are also frequently described. Moreover, driving under the influence of alcohol has been widely described as a major predisposing factor. We conducted a retrospective cohort study to categorize risk factors, including demographic information and alcohol/psychoactive substance consumption, as well as pre-existing medical conditions. Correspondingly, we identified the most common injury patterns and attempted to establish a connection between time to hospital and survival rates. Methods: Cases of motorcycle-related road traffic accidents (RTAs) examined by the authors during 2021–2023 were included in the study sample (94 cases in total). This retrospective cohort study assessed survival time (in days) from accident to death. Kaplan–Meier curves, stratified by key categorical variables, were used to analyze survival probabilities over time. Univariate Cox regression was used to assess each variable’s effect on survival. The association between exposure and mortality was analyzed using hazard ratios (HRs) and 95% confidence intervals (CIs). Results: Head injuries were associated with poorer outcomes. Chest injuries reduced median survival to 1.68 h compared to 5.85 days in cases that had not sustained chest injuries. Abdominal injuries also shortened survival (1.632 h vs. 1.896 h), as did multiple-site injuries (1.584 h vs. 0.2 days for single/double-site). Positive toxicology for psychoactive substances lowered survival to 1.32 h compared to 1.752 h in cases with negative toxicological findings. Multiple-site injuries and head, chest and abdominal injuries appeared to significantly affect the survival of victims. Positive toxicological examination results for psychoactive substances also appeared to heavily impact survival. Full article

Background/Objectives: Establishing the identity of unknown individuals has always been one of the primary objectives of anthropologists and forensic pathologists in judicial contexts. Particularly when human remains are found in advanced stages of decomposition, carbonization, or fragmentation conditions that may compromise the efficacy of techniques such as DNA analysis or dental comparison innovative methodologies, including craniofacial superimposition, are employed, often supplemented by further examinations. This study presents the discovery of an individual in an advanced state of decomposition, transitioning from the colliquative to the semi-skeletal phase, demonstrating how degenerative processes can alter soft tissues to the extent of hindering genetic investigations. Methods: The multidisciplinary investigation conducted to resolve the case is described in two phases: the first, of an anthropological and medico-legal nature, aimed at reconstructing the biological profile (sex, age, stature, ancestry); the second, anthropological in focus, directed toward identification through craniofacial superimposition, applying two established methods from the literature the linear method and the computer-assisted comparison approach. Results: The results obtained from both investigative phases proved decisive, providing a significant and anticipated resolution for the authorities involved. Conclusions: This judicial case ultimately reaffirms the critical importance of multidisciplinary collaboration in forensic investigations. Full article

Background/Objectives: Determination of the biological profile, particularly sex and stature, constitutes an essential component for individual identification in forensic and archaeological anthropology; however, validation of anthropometric equations remains inadequately implemented in contemporary research. This study addresses two limitations: the isolated development of sex and stature estimation methods and the lack of rigorous validation using independent samples. Methods: In this research, we analyzed 400 well-preserved scapulae from a Northeastern Thai population divided into training (n = 300: 150 male scapulae, 150 female scapulae) and validation (n = 100: 50 male scapulae, 50 female scapulae) groups. Eight standardized measurements were used for both sex and stature estimation. Results: All measurements demonstrated significant sexual dimorphism, with males exhibiting larger dimensions. For sex estimation, a multivariate model incorporating the maximum scapular height, maximum length of the spine, and scapula weight achieved 96.3% accuracy in the training samples and maintained 95.0% accuracy in independent validation. For stature estimation, a three-variable equation combining scapula weight, longitudinal scapular length, and maximum scapular breadth was strongly correlated (R = 0.769, SEE = 5.32 cm) with consistent performance across validation samples. Conclusions: Validation testing confirmed the high accuracy, reliability, and stability of both equations when applied to independent samples, with no significant differences in performance metrics between training and validation groups. These validated equations provide reliable standards for forensic practitioners analyzing scapular remains in practical applications within the northeastern Thai population. Full article

Background: The analysis of gunshot residue (GSR) is crucial for gaining information on how a crime occurred. This study presents an innovative proof of concept for measuring shooting distances by performing Flash-Pulse active Thermography (FPT). Compared to conventional chemical methods, FPT offers a significant advantage by digitalizing the residue pattern in a non-destructive manner. Methods: Thermal images of cotton canvases, both white and colored, were analyzed to quantify the distribution of gunshot residues after shooting from several distances, specifically focusing on smoke and semi-burnt powders. The proposed approach uses contrast and radial intensity profiles to extract exponential coefficients, which are dependent on the shooting distance. Results: Employing a sigmoid model to fit the coefficients over distance and to derive a characteristic feature used as a classification metric, firing distances can be classified into short, medium, and long range and can be predicted with an uncertainty of less than 5 cm for distances between 18 and 38 cm under the tested conditions. Considerations regarding the influence of different weapons and ammunition are reported, suggesting the potential for a general approach. Conclusions: The methodology has been validated on several samples, demonstrating its feasibility for specific forensic applications. Its most robust use is as a weapon- and ammunition-specific calibration tool, supporting case-specific distance estimation analysis. Full article

Background: The DASH risk assessment scheme is used across the UK to identify and manage instances of domestic abuse. Recent studies have questioned whether the scheme can identify offenders who go on to commit further acts of domestic abuse, in particular serious violence, and therefore whether it is fit for purpose. Methods: We therefore tested the ability of the DASH to predict future instances of deadly or persistent domestic abuse. From a database of ≈25,000 incidents, we compared DASH assessments which preceded an incident of “deadly violence” or was the first in a series of “persistent abuse”. These groups were compared to a control group where there was no further incident of domestic abuse. Results: The proportion of “high-risk” stratifications was approximately 5 times higher in the deadly violence group compared to the control group. Prediction accuracy assessed via signal detection theory showed the DASH was a moderate predictor of deadly violence (AUC = 0.67). The DASH also showed predictive accuracy in identifying persistent offenders (AUC = 0.62). While these results are encouraging and are similar in efficacy to other risk assessment schemes used in the prediction of domestic violence, the results identified that many individual items of the DASH were not predictive. The inclusion of non-predictive items within the DASH adds “noise” and error into the risk evaluation. The development of a shortened version of the DASH, removing these ineffectual items, was shown to have even higher predictive value for deadly violence (AUC = 0.80). Conclusions: We stress, however, that the role of risk assessment is not to predict violence per se, but to prevent violence via the accurate identification of dangerous perpetrators and via effective intervention and safeguarding of victims. Despite this, research such as this is imperative to evaluate if the risk assessment schemes selected by practitioners and police are fit for purpose. Full article

Background/Objectives: Chronic pain in women arises from a complex interplay of biological, psychological, and social factors. Despite its impact, validated screens for these bio-psycho-social risk factors are lacking. This study aimed to develop and validate a new screening and assessment tool to prevent chronic pain onset and detect hidden experiences of violence or trauma in women, also supporting individualized treatment. Methods: Conducted from December 2023 to June 2024 as part of a larger project, the original instrument comprised 36 bio-psycho-social risk factors. Rasch analysis was used for validation, assessing Infit, Outfit, DIF, ROC curves, and reliability indexes. Results: The sample included 239 women (100 victims), with 103 experiencing chronic pain, and 136 pain-free. Seven items were excluded due to poor fit, resulting in a 29-item version that met Rasch model criteria. Conclusions: The validated 29-item checklist, available in Italian, supports the bio-psycho-social model by identifying risk factors for chronic pain onset and detecting violence-related variables in diagnosed individuals. It offers clinicians and operators a practical tool to guide prevention and tailor personalized treatments. In addition, the RFCP-CK holds forensic relevance by offering measurable indicators that can support medico-legal evaluations, especially in cases where chronic pain is claimed to be a consequence of violence. Full article

Background/Objectives: Toolmark analysis on cartridge cases offers critical insights in forensic ballistics, as the impressions left on cartridge cases by firearm components—such as the firing pin, breech face, and annulus—carry distinctive patterns and act as unique identifiers that can be used for firearm linkage. This study aims to develop a systematic and interpretable feature extraction pipeline for these regions to support future automation and comparison studies in forensic cartridge case analysis. Methods: A dataset of 20 high-resolution cartridge case images was prepared, and each region of interest (firing pin impression, breech face, and annulus) was manually annotated using the LabelMe tool. ImageJ and Python-based scripts were employed for feature extraction, capturing geometric descriptors (area, perimeter, circularity, and eccentricity) and texture-based features (Local Binary Patterns and Haralick statistics). In total, 61 quantitative features were derived from the annotated regions. Similarity between cartridge cases was evaluated using Euclidean distance metrics after normalization. Results: The extracted and calibrated region-wise geometric and texture features demonstrated distinct variation patterns across firing pin, breech face, and annulus regions. Pairwise similarity analysis revealed measurable intra-class differences, indicating the discriminative potential of the extracted features even within cartridges likely fired from the same firearm. Conclusions: This study provides a foundational, region-wise quantitative framework for analysing cartridge case impressions. The extracted dataset and similarity outcomes establish a baseline for subsequent research on firearm identification and model-based classification in forensic ballistics. Full article

Background: Cancer-related cachexia remains a significant cause of death, particularly for undiagnosed or untreated malignancies. Lymphomas, especially in uncommon locations, may go unrecognized until their advanced stages. Methods: We report the case of a 34-year-old woman who died from cancer-related cachexia due to undiagnosed, untreated cervical non-Hodgkin lymphoma. Four months postpartum, she reported having excluded malignancy through medical investigations, which were later confirmed to have never been performed. The Judicial Authority ordered an autopsy to determine the cause of death. A narrative literature review was conducted via PubMed using the terms “Non-Hodgkin Lymphoma” and “Autopsy,” limited to English-language human studies published between January 2000 and February 2025. Results: At autopsy, marked fat depletion and a 1350 g cervical mass were found, with significant anatomical distortion and airway narrowing due to epiglottic edema. Microscopic examination identified a diffuse large B-cell lymphoma of germinal center origin. A literature review on lymphoma-related autopsy findings identified common diagnostic challenges, including nonspecific symptoms, rapid clinical deterioration, the rarity of certain subtypes, and a lack of medical compliance. Conclusions: Early recognition and proper investigation of lymphoproliferative disorders are crucial to prevent fatal outcomes. Postmortem findings can offer valuable insights into missed diagnoses and inform strategies to reduce diagnostic delay. Full article

Background: Sex estimation is a basic step of human identification in both legal cases and archeological contexts. The highest accuracy for sex estimation is achieved when a complete skeleton is available, though there are situations, such as cremated, dismembered, and otherwise taphonomically altered skeletal remains, where a complete skeleton is not available. The aim of the present preliminary study was to evaluate the usefulness of four non-metric skull traits that are considered taphonomically resilient for sex estimation and their potential application in forensic cases. Methods: Non-metric skull traits of 100 skulls from the Bass Donated Skeletal Collection were analyzed. These traits included foramen magnum shape, zygomatic arch extension with respect to the external auditory canal, sigmoid notch, and gonial angle muscle attachment. A discriminant function analysis model was used to develop specific formulae for sex estimation. Results: The foramen magnum and sigmoid notch showed no significant differences between males and females. The zygomatic arch extension (ZAE) and gonial angle morphology (GO) showed strong, significant differences between the sexes. However, gonial angle morphology has shown to be affected by edentulism. Based on the ZAE, the function obtained by the discriminant function analysis was sex = 2.469*ZAE − 1.247, with a result of zero pointing to males and result of one pointing to females, which correctly classified 79.8% of the original cases. Conclusions: This study highlights the value of four different skull traits and their potential use in forensic cases. Of all the evaluated traits, zygomatic arch extension was the best indicator for sex estimation. This anatomical region corresponds to a highly resistant skeletal structure. Full article

Background/Objectives: The Commission d’examen des troubles mentaux (CETM), under Quebec’s Tribunal Administratif du Québec, reviews individuals found not criminally responsible on account of mental disorder (NCRMD). These hearings seek to balance public safety with reintegration, guided largely by treatment team recommendations. Despite the CETM’s central role in forensic psychiatry, limited empirical data exist on how its decisions align with clinical advice and which dynamic risk factors influence outcomes. This study aimed to (1) profile the CETM’s 2023 caseload, (2) evaluate concordance between CETM dispositions and treatment team recommendations, and (3) examine clinical, social, and legal factors associated with decision-making. Methods: We conducted a retrospective review of 1721 judgments issued by the CETM in 2023, retrieved from the publicly accessible Société Québécoise d’information juridique (SOQUIJ) database. Eligible cases included annual NCRMD review hearings, excluding trial fitness assessments and repeated hearings within the same year. A structured coding grid documented sociodemographic, administrative, legal, and clinical information, with emphasis on dynamic risk factors such as treatment adherence, substance use, and recent aggression. Descriptive analyses summarized population characteristics and concordance between clinical recommendations and CETM decisions. Results: The cohort was predominantly male (85%) with a mean age of 41 years. Psychotic disorders were the most frequent primary diagnoses (76%), frequently accompanied by substance use and antisocial traits. Most patients (79.6%) had prior psychiatric hospitalizations, while 25.5% had prior incarcerations. Nearly half displayed recent aggression or non-compliance. Treatment teams most often recommended conditional discharge (55%), followed by detention with conditions (21%) and unconditional release (19%). CETM decisions aligned with recommendations in 83.6% of cases; when divergent, rulings were more restrictive (8.6%) than permissive (4.6%). Conclusions: This study provides the first large-scale profile of Quebec’s CETM. High concordance with clinical teams was observed, but restrictive decisions were more frequent in cases of disagreement. The findings underscore the importance of incorporating standardized risk assessment tools to enhance transparency, consistency, and balance in forensic decision-making. Full article

Background: The present exploratory study aimed to investigate the relationship between arginine vasopressin (AVP) secretion and cerebral edema and evaluate its usefulness as an index for assessing severity of cerebral edema. Methods: Forensic autopsy was performed within 96 h after death in 143 cases, and samples of right heart blood and cerebrospinal fluid (CSF) were collected. Serum AVP levels and CSF were then measured and relationships with brain weight (BW) and normalized BW were investigated. Results: No relationships between serum AVP levels or CSF and age, sex, survival, or postmortem period were identified. A comparison in regard to cause of death revealed lower CSF AVP concentrations in cases of blunt head injury and cerebrovascular disease. In addition, a correlation was observed between serum AVP levels and non-normalized or normalized BW with blunt head injury or asphyxia. Receiver operating characteristic analysis revealed that neither serum nor CSF AVP concentrations yielded cutoff values readily applicable to the diagnosis of cerebral edema. Conclusions: The present findings suggest that postmortem AVP concentrations remain relatively stable and may be involved in the formation of cerebral edema. The findings also highlighted issues such as the influence of confounding factors associated with individual pathologies and the verification of AVP stability in postmortem samples. Thus, the usefulness of AVP as an indicator of cerebral edema in forensic evaluation remains unclear. Full article

Background/Objectives: Incomplete cut marks produced during dismemberment are often interpreted as indicative of saw class characteristics. However, empirical validation of these associations remains limited, with prior studies examining six or fewer saws. Considering the wide variety of saws available, it is critical to assess the reliability of reported relationships between kerf features and saw classification using a larger sample, particularly in light of the serious legal consequences of erroneous conclusions. This study examines the statistical relationships between five incomplete cut traits—kerf profile shape (KPS), kerf length shape (KLS), floor dip (FD), kerf flare (KF), and floor striae (FS)—and saw class characteristics, including tooth set, tooth shape, teeth-per-inch, power, handle orientation, and cut direction. Methods: Kerf features were scored on a sample of 472 incomplete cuts made with 34 power and hand saws. Results: In reciprocating saws, W-shaped KPS was exclusively associated with crosscut, alternating saws (100%; p < 0.001), with hourglass-shaped KLS also primarily made by alternating sets (95.6%). Necked KLS was linked to wavy sets (76.8%; p < 0.001). FD, though rare, could be correctly assigned to teeth-per-inch groups (86.4%), and was also predominantly associated with alternating saws (90.9%; p < 0.001). Undulating FS were indicative of alternating saws with less than 20 teeth-per-inch (100%, p < 0.001). In contrast, KF showed no strong relationship with saw class characteristics, including handle side. Conclusions: The results of this large-scale analysis support most reported relationships in the saw mark literature but challenge assumptions that KF reliably indicates handle orientation or cut direction, suggesting instead that its location may reflect sawyer technique. Full article

Background/Objectives: This study investigated the use of artificial intelligence (AI) to identify and match barefoot prints belonging to the same individual on soft and sandy soil substrates. Recognizing footprints on soil is challenging due to low contrast and variability in impressions. Methods: We introduce Deep Learning Footprint Identification Technology (DeepFIT), based on a modified You Only Look Once (YOLOv11s) algorithm, using three methods, namely, Bounding Box (BBox), 16 anatomical landmarks, and automatically segmented outlines (Auto-Seg). An Extra Small Detection Head (XSDH) was added to improve feature extraction at smaller scales and enhance generalization through multi-scale supervision, reducing overfitting to specific spatial patterns. Results: Forty adults (20 males, 20 females) participated, with 600 images per individual. As the number of individuals in model training increased, the BBox model’s accuracy declined, resulting in misclassification on the test set. The average performance accuracy across both substrates was 77% for BBox, 90% for segmented outlines, and 96% for anatomical landmarks. Conclusions: The landmark method was the most reliable for identifying and matching barefoot prints on both soft and sandy soils. This approach can assist forensic practitioners in linking suspects to crime scenes and reconstructing events from footprint evidence, providing a valuable tool for forensic investigations. Full article

Background/Objectives: Overkill is generally defined as violence demonstrably exceeding the force required to cause death, often involving a disproportionate number of inflicted injuries. Typically linked to specific mental states, this phenomenon can serve diverse perpetrator purposes, from expressing sadistic impulses to deliberately contaminating forensic evidence. This study re-examines the historical case of Mary Jane Kelly, the canonical final victim of Jack the Ripper, to offer a historical perspective on extreme violence and its influence on modern forensic frameworks. Methods: We employed a retrospective analysis, combining a systematic literature review with application of the Homicide Injury Scale to documented autopsy findings from 1888. Results: The autopsy revealed systematic post-mortem mutilation following a fatal neck laceration. Application of the Homicide Injury Scale confirmed violence exceeding lethal force. Conclusions: This case represents a foundational example of organized overkill behavior, demonstrating the value of applying modern forensic frameworks to historical cases to inform comprehensive overkill analysis. Full article