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501 Results Found

  • Review
  • Open Access
1 Citations
1,775 Views
52 Pages

Conventional wastewater treatment methods typically achieve 70–90% removal efficiency for organic pollutants. However, the global wastewater crisis—with 80% of wastewater discharged untreated—demands innovative solutions to overcome...

  • Article
  • Open Access
2,549 Views
11 Pages

An In Vitro Artificial Wound Slough–Biofilm Model Developed for Evaluating a Novel Antibiofilm Technology

  • Rui Chen,
  • Jeanne Saint Bezard,
  • Marcus J. Swann,
  • Fergus Watson and
  • Steven L. Percival

Eschar and slough in wounds serve as a reservoir for microorganisms and biofilms, damaged/devitalised cells, and inflammatory chemokines/cytokines, which work to initiate and prolong persistent inflammation and increase the risk of infection. Biofilm...

  • Article
  • Open Access
3 Citations
2,399 Views
14 Pages

8 December 2023

This study investigates the water-saving and emission-reduction effects of biofilm technology on the pond culture of largemouth bass (Micropterus pallidus) and Japanese eel (Anguilla japonica) using a combination of biofilm water purification grids a...

  • Article
  • Open Access
1 Citations
1,738 Views
11 Pages

7 March 2024

Background: This in vitro study set out to find out how well oral cavity-dwelling bacteria can form biofilms and adhere on the surfaces of zirconium oxide samples created by 3D printing and milling technologies. Methods: 5 strains of microorganisms w...

  • Article
  • Open Access
4 Citations
2,457 Views
21 Pages

1 June 2023

The northwest of China has a shortage of water resources, and classic sewage treatment techniques have a high cost and produce waste nitrogen and phosphorus. In order to achieve inexpensive discharge standards, an anaerobic biofilm coupled with ABR t...

  • Article
  • Open Access
15 Citations
3,923 Views
28 Pages

In-Vitro Biofilm Removal Efficacy Using Water Jet in Combination with Cold Plasma Technology on Dental Titanium Implants

  • Rutger Matthes,
  • Lukasz Jablonowski,
  • Lea Miebach,
  • Vinay Pitchika,
  • Birte Holtfreter,
  • Christian Eberhard,
  • Leo Seifert,
  • Torsten Gerling,
  • Rabea Schlüter and
  • Sander Bekeschus
  • + 1 author

13 January 2023

Peri-implantitis-associated inflammation can lead to bone loss and implant failure. Current decontamination measures are ineffective due to the implants’ complex geometry and rough surfaces providing niches for microbial biofilms. A modified wa...

  • Article
  • Open Access
43 Citations
4,254 Views
13 Pages

Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study

  • Felice Panebianco,
  • Selene Rubiola,
  • Francesco Chiesa,
  • Tiziana Civera and
  • Pierluigi Aldo Di Ciccio

26 June 2021

Among food-borne pathogens, Listeria monocytogenes continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but...

  • Review
  • Open Access
60 Citations
7,119 Views
18 Pages

Managing spoilage and pathogenic bacteria contaminations represents a major challenge for the food industry, especially for the dairy sector. Biofilms formed by these microorganisms in food processing environment continue to pose concerns to food man...

  • Article
  • Open Access
38 Citations
5,273 Views
13 Pages

10 April 2023

The aim of this study was to evaluate the adhesion and biofilm formation of Candida albicans (C. albicans) on conventionally fabricated, milled, and 3D-printed denture base resin materials in order to determine the susceptibility of denture contamina...

  • Review
  • Open Access
7 Citations
6,441 Views
21 Pages

Integrated Application of Biofloc Technology in Aquaculture: A Review

  • Changwei Li,
  • Zhenbo Ge,
  • Limin Dai and
  • Yuan Chen

15 July 2025

Although biofloc technology (BFT) currently offers advantages such as improving aquaculture water quality, providing natural bait for cultured animals, and reducing pests and diseases, single BFT systems face technical bottlenecks, including the comp...

  • Article
  • Open Access
1,561 Views
11 Pages

Quaternary Ammonium Silica Nanoparticles for Antimicrobial Implantable Medical Devices: An In Vitro Study

  • Eitam Weiss,
  • Ariel Berl,
  • Ofir Shir-az,
  • Biader Samih Bilal,
  • Ervin I. Weiss,
  • Yossi Paitan,
  • Natan Zaltsman,
  • Alexander Golberg and
  • Avshalom Shalom

12 December 2024

Biofilm formation on prostheses and implanted devices can lead to serious complications and increased healthcare expenditures. Once formed, biofilm management is difficult and may involve a long course of antibiotics, additional surgery, and, occasio...

  • Review
  • Open Access
51 Citations
11,654 Views
17 Pages

Shifting from Conventional to Organic Filter Media in Wastewater Biofiltration Treatment: A Review

  • Zhang Zhan Loh,
  • Nur Syamimi Zaidi,
  • Achmad Syafiuddin,
  • Ee Ling Yong,
  • Raj Boopathy,
  • Ahmad Beng Hong Kueh and
  • Dedy Dwi Prastyo

17 September 2021

Biofiltration is a promising wastewater treatment green technology employed to remove various types of pollutants. The efficiency of biofiltration relies on biofilm, and its performance is significantly influenced by various factors such as dissolved...

  • Review
  • Open Access
19 Citations
8,472 Views
12 Pages

11 November 2013

Investigations of microbial ecology and diversity have been greatly enhanced by the application of culture-independent techniques. One such approach, metagenomics, involves sample collections from soil, water, and other environments. Extracted nuclei...

  • Review
  • Open Access
12 Citations
6,566 Views
12 Pages

The application of seawater desalination technology using a reverse osmosis (RO) membrane has been expanding because it requires less energy compared with other distillation methods. Even in Middle Eastern countries where energy costs are lower such...

  • Review
  • Open Access
126 Citations
29,370 Views
18 Pages

28 August 2020

Selection of appropriate river water treatment methods is important for the restoration of river ecosystems. An in-depth review of different river water treatment technologies has been carried out in this study. Among the physical-engineering process...

  • Article
  • Open Access
15 Citations
4,044 Views
14 Pages

10 August 2020

Phosphorus is one of the non-renewable natural resources. High concentration of phosphorus in surface water leads to undesirable eutrophication of the water ecosystem. It is therefore necessary to develop new technologies not only for capturing phosp...

  • Review
  • Open Access
13 Citations
5,180 Views
22 Pages

Microbial Ecology of Granular Biofilm Technologies for Wastewater Treatment: A Review

  • Aurora Rosa-Masegosa,
  • Alejandro Rodriguez-Sanchez,
  • Susanna Gorrasi,
  • Massimiliano Fenice,
  • Alejandro Gonzalez-Martinez,
  • Jesus Gonzalez-Lopez and
  • Barbara Muñoz-Palazon

Nowadays, the discharge of wastewater is a global concern due to the damage caused to human and environmental health. Wastewater treatment has progressed to provide environmentally and economically sustainable technologies. The biological treatment o...

  • Review
  • Open Access
38 Citations
12,579 Views
27 Pages

Archaeal Diversity in Biofilm Technologies Applied to Treat Urban and Industrial Wastewater: Recent Advances and Future Prospects

  • Kadiya Calderón,
  • Alejandro González-Martínez,
  • Cinta Gómez-Silván,
  • Francisco Osorio,
  • Belén Rodelas and
  • Jesús González-López

9 September 2013

Biological wastewater treatment (WWT) frequently relies on biofilms for the removal of anthropogenic contaminants. The use of inert carrier materials to support biofilm development is often required, although under certain operating conditions microo...

  • Article
  • Open Access
11 Citations
3,310 Views
16 Pages

New Adapted In Vitro Technology to Evaluate Biofilm Formation and Antibiotic Activity Using Live Imaging under Flow Conditions

  • Cassandra Pouget,
  • Catherine Dunyach-Remy,
  • Alix Pantel,
  • Sophie Schuldiner,
  • Albert Sotto and
  • Jean-Philippe Lavigne

23 September 2021

The polymicrobial nature of biofilms and bacterial interactions inside chronic wounds are keys for the understanding of bacterial cooperation. The aim of this present study was to develop a technique to study and visualize biofilm in live imaging und...

  • Article
  • Open Access
15 Citations
5,479 Views
13 Pages

A Minimally Invasive Microsensor Specially Designed for Simultaneous Dissolved Oxygen and pH Biofilm Profiling

  • Xavier Guimerà,
  • Ana Moya,
  • Antonio David Dorado,
  • Xavi Illa,
  • Rosa Villa,
  • David Gabriel,
  • Xavier Gamisans and
  • Gemma Gabriel

1 November 2019

A novel sensing device for simultaneous dissolved oxygen (DO) and pH monitoring specially designed for biofilm profiling is presented in this work. This device enabled the recording of instantaneous DO and pH dynamic profiles within biofilms, improvi...

  • Article
  • Open Access
14 Citations
4,020 Views
10 Pages

Organo-Selenium-Containing Polyester Bandage Inhibits Bacterial Biofilm Growth on the Bandage and in the Wound

  • Phat Tran,
  • Tyler Enos,
  • Keaton Luth,
  • Abdul Hamood,
  • Coby Ray,
  • Kelly Mitchell and
  • Ted W. Reid

The dressing material of a wound plays a key role since bacteria can live in the bandage and keep re-infecting the wound, thus a bandage is needed that blocks biofilm in the bandage. Using an in vivo wound biofilm model, we examined the effectiveness...

  • Article
  • Open Access
9 Citations
4,307 Views
13 Pages

Plasma-treated water (PTW) has emerged as a potential sanitizing agent. This study evaluated antibacterial activity, inhibition of invasion, and biofilm disruption effects of PTW against Salmonella Typhimurium. Minimum inhibitory concentrations (MICs...

  • Feature Paper
  • Article
  • Open Access
13 Citations
13,160 Views
10 Pages

Heterotrophic Plate Count for Bottled Water Safety Management

  • Anna Rygala,
  • Joanna Berlowska and
  • Dorota Kregiel

24 June 2020

Heterotrophic bacteria are able to form biofilms in water processing systems, adhering to pipe materials and colonizing surfaces. The aim of our research was to identify the critical points in the process of bottled water production at which controls...

  • Article
  • Open Access
6 Citations
2,159 Views
21 Pages

Naringenin-Loaded Solid Lipid Nanoparticles: Physical–Chemical Characterization and In Vitro Antibacterial Activity

  • Federica De Gaetano,
  • Francesco Caridi,
  • Noemi Totaro,
  • Consuelo Celesti,
  • Valentina Venuti,
  • Giovanna Ginestra,
  • Antonia Nostro,
  • Silvana Tommasini,
  • Cinzia Anna Ventura and
  • Rosanna Stancanelli

8 February 2025

Currently, problems related to antibiotic resistance are shifting the focus of pharmaceutical research towards natural molecules with antibacterial properties. Among them, flavonoids represent promising molecules with strong antibacterial features; h...

  • Review
  • Open Access
2 Citations
2,327 Views
35 Pages

Strategies for Biofouling Control: A Review from an Environmental Perspective of Innovation and Trends

  • Virgínia Rayanne Soares de Souza,
  • Camila Ferreira Alves,
  • Larissa Felix de Lucena,
  • Luana Caroline Costa Silva,
  • Everthon de Albuquerque Xavier,
  • Cláudio José Galdino da Silva Jr.,
  • Attilio Converti,
  • Renata Laranjeiras Gouveia and
  • Leonie Asfora Sarubbo

9 October 2025

Biofouling is the colonization and attachment of sessile organisms on submerged surfaces, whether natural or artificial. The presence of these communities compromises the structural integrity, operational efficiency, and durability of coastal structu...

  • Review
  • Open Access
384 Citations
17,861 Views
14 Pages

6 September 2013

Biofilm formation by human bacterial pathogens on implanted medical devices causes major morbidity and mortality among patients, and leads to billions of dollars in healthcare cost. Biofilm is a complex bacterial community that is highly resistant to...

  • Review
  • Open Access
12 Citations
7,249 Views
34 Pages

Targeted Anti-Biofilm Therapy: Dissecting Targets in the Biofilm Life Cycle

  • Fanqiang Bu,
  • Mengnan Liu,
  • Zixu Xie,
  • Xinyu Chen,
  • Guofeng Li and
  • Xing Wang

12 October 2022

Biofilm is a crucial virulence factor for microorganisms that causes chronic infection. After biofilm formation, the bacteria present improve drug tolerance and multifactorial defense mechanisms, which impose significant challenges for the use of ant...

  • Communication
  • Open Access
1 Citations
2,609 Views
9 Pages

Developing an Electrical System toward the Prevention of Heat Ventilation Air Conditioning Contamination

  • Sang Kuy Han,
  • Young Wook Kim,
  • Bon-Sang Koo,
  • Hae Won Choi and
  • Soonbum Lee

21 November 2022

Biofilms comprise diverse bacteria and fungi with slime material, especially in humid conditions. The surface of the heat ventilation and air conditioning (HVAC) system, especially at the evaporator, is in high moisture conditions due to the compress...

  • Review
  • Open Access
5 Citations
3,784 Views
22 Pages

Insights into Aeration Intensification in Biofilm Reactors for Efficient Wastewater Treatment

  • Hassimi Abu Hasan,
  • Nur Asyiqin Azahar and
  • Mohd Hafizuddin Muhamad

23 June 2025

Aeration used in wastewater treatment is energy-intensive, subsequently increasing the cost of treatment. Aeration is used to supply oxygen that is required for bacterial metabolism that degrades organic compounds in wastewater. In this review, we wi...

  • Article
  • Open Access
1 Citations
1,373 Views
15 Pages

Characterization and Multi-Omics Basis of Biofilm Formation by Lactiplantibacillus plantarum

  • Ruitang Ma,
  • Dong Zhao,
  • Rongqing Zhou,
  • Jia Zheng and
  • Chongde Wu

Lactiplantibacillus plantarum is a kind of common lactic acid bacteria, which plays an important role in the production of fermented foods. In general, the formation of biofilm is conducive to the adaptability of cells in the face of fierce competiti...

  • Article
  • Open Access
10 Citations
6,679 Views
24 Pages

A Reproducible Method for Growing Biofilms on Polystyrene Surfaces: Biomass and Bacterial Viability Evolution of Pseudomonas fluorescens and Staphylococcus epidermidis

  • Valeria Angarano,
  • Cindy Smet,
  • Simen Akkermans,
  • Theodora Akritidou,
  • Bart Huyck,
  • Andre Chieffi and
  • Jan F. M. Van Impe

30 June 2020

Since biofilm development represents a crucial issue within industrial, clinical and domestic sectors, innovative technologies/approaches (e.g., light technology for inactivation, antibiofilm coatings) are required to eradicate them. In this multidis...

  • Review
  • Open Access
12 Citations
4,384 Views
23 Pages

Advances in the Application of Quorum Sensing to Regulate Electrode Biofilms in Bioelectrochemical Systems

  • Shen Wang,
  • Xinglei Zhuang,
  • Weiliang Dong,
  • Fengxue Xin,
  • Honghua Jia and
  • Xiayuan Wu

Bioelectrochemical systems (BESs) are an emerging technology for wastewater treatment and resource recovery. These systems facilitate electron transfer between microorganisms and electrodes, enabling their application in various fields, such as elect...

  • Review
  • Open Access
662 Citations
83,427 Views
32 Pages

Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment

  • Satish Sharma,
  • James Mohler,
  • Supriya D. Mahajan,
  • Stanley A. Schwartz,
  • Liana Bruggemann and
  • Ravikumar Aalinkeel

Biofilm is complex and consists of bacterial colonies that reside in an exopolysaccharide matrix that attaches to foreign surfaces in a living organism. Biofilm frequently leads to nosocomial, chronic infections in clinical settings. Since the bacter...

  • Brief Report
  • Open Access
6 Citations
2,735 Views
12 Pages

A Non-Electrolysis Bioelectric Effect for Gingivitis and Hygiene Contamination Biofilm Removal

  • Young Wook Kim,
  • Jihyun Lee,
  • Sang Kuy Han,
  • Bon-Sang Koo,
  • Taeguen Park,
  • Hyun Mok Park and
  • Byoungdoo Lee

A combinatorial biofilm treatment involving a low dose of antibiotics along with a small amount of electricity is known as the bioelectric effect (BE). When an external electric field or current is applied, biofilms can be affected by the resulting e...

  • Review
  • Open Access
303 Citations
21,258 Views
14 Pages

Biofilm Forming Lactobacillus: New Challenges for the Development of Probiotics

  • María José Salas-Jara,
  • Alejandra Ilabaca,
  • Marco Vega and
  • Apolinaria García

Probiotics are live bacteria, generally administered in food, conferring beneficial effects to the host because they help to prevent or treat diseases, the majority of which are gastrointestinal. Numerous investigations have verified the beneficial e...

  • Article
  • Open Access
13 Citations
3,794 Views
10 Pages

8 April 2020

Biofouling is inevitable in wastewater reclamation when using membrane technology. In particular, the extracellular polymeric substances (EPS) from biofilm is a major contributor to biofouling. Coagulation is critical in the process of reusing wastew...

  • Review
  • Open Access
4 Citations
6,059 Views
46 Pages

Biofilms Exposed: Innovative Imaging and Therapeutic Platforms for Persistent Infections

  • Manasi Haval,
  • Chandrashekhar Unakal,
  • Shridhar C. Ghagane,
  • Bijay Raj Pandit,
  • Esther Daniel,
  • Parbatee Siewdass,
  • Kingsley Ekimeri,
  • Vijayanandh Rajamanickam,
  • Angel Justiz-Vaillant and
  • Pramod Bhasme
  • + 5 authors

Biofilms constitute a significant challenge in the therapy of infectious diseases, offering remarkable resistance to both pharmacological treatments and immunological elimination. This resilience is orchestrated through the regulation of extracellula...

  • Review
  • Open Access
37 Citations
6,896 Views
36 Pages

A Technological Understanding of Biofilm Detection Techniques: A Review

  • Spyridon Achinas,
  • Stijn Keimpe Yska,
  • Nikolaos Charalampogiannis,
  • Janneke Krooneman and
  • Gerrit Jan Willem Euverink

15 July 2020

Biofouling is a persistent problem in almost any water-based application in several industries. To eradicate biofouling-related problems in bioreactors, the detection of biofilms is necessary. The current literature does not provide clear supportive...

  • Article
  • Open Access
17 Citations
4,244 Views
15 Pages

Biofilm commonly forms on the surfaces of cellulosic biomass but its roles in cellulose degradation remain largely unexplored. We used Bacillus subtilis to study possible mechanisms and the contributions of two major biofilm components, extracellular...

  • Review
  • Open Access
23 Citations
4,539 Views
20 Pages

26 October 2021

Biofilms cause problems in the food industry due to their persistence and incompetent hygiene processing technologies. Interest in photodynamic inactivation (PDI) for combating biofilms has increased in recent years. This technique can induce microbi...

  • Feature Paper
  • Review
  • Open Access
76 Citations
12,251 Views
8 Pages

Oral Biofilms: Development, Control, and Analysis

  • Daniela Berger,
  • Aviva Rakhamimova,
  • Andrew Pollack and
  • Zvi Loewy

31 August 2018

The oral cavity harbors hundreds of microbial species that are present either as planktonic cells or incorporated into biofilms. The majority of the oral microbes are commensal organisms. Those that are pathogenic microbes can result in oral infectio...

  • Article
  • Open Access
3 Citations
2,356 Views
17 Pages

Sublethal Damage Caused by Cold Plasma on Bacillus cereus Cells: Impact on Cell Viability and Biofilm-Forming Capacity

  • Laura Eced-Rodríguez,
  • Michael Beyrer,
  • Dolores Rodrigo,
  • Alejandro Rivas,
  • Consuelo Esteve and
  • Maria Consuelo Pina-Pérez

13 October 2024

The Bacillus cereus group represents a serious risk in powdered and amylaceous foodstuffs. Cold plasma (the fourth state of matter) is emerging as an alternative effective nonthermal technology for pasteurizing a wide range of matrices in solid, liqu...

  • Article
  • Open Access
67 Citations
5,998 Views
12 Pages

Potential of Carvacrol and Thymol in Reducing Biofilm Formation on Technical Surfaces

  • Maciej Walczak,
  • Marta Michalska-Sionkowska,
  • Daria Olkiewicz,
  • Patrycja Tarnawska and
  • Oliwia Warżyńska

Polyvinyl chloride (PVC), polypropylene (PP), polyethylene (PE), and stainless steel (SS) are commonly used in medicine and food production technologies. During contact with microorganisms on the surface of these materials, a microbial biofilm is for...

  • Review
  • Open Access
14 Citations
6,413 Views
22 Pages

Electrochemical Control of Biofilm Formation and Approaches to Biofilm Removal

  • Iveta Brožková,
  • Libor Červenka,
  • Petra Moťková,
  • Michaela Frühbauerová,
  • Radovan Metelka,
  • Ivan Švancara and
  • Milan Sýs

21 June 2022

This review deals with microbial adhesion to metal-based surfaces and the subsequent biofilm formation, showing that both processes are a serious problem in the food industry, where pathogenic microorganisms released from the biofilm structure may po...

  • Review
  • Open Access
11 Citations
5,515 Views
13 Pages

18 January 2023

The growth of biofilm, which is caused by microorganism accumulation and growth on wetted surfaces, may damage industrial piping systems, increase maintenance and cleaning costs for the system sterilization, and even divulge the immune system into hi...

  • Review
  • Open Access
3 Citations
2,857 Views
16 Pages

Biofilm formation is important for microbial survival, adaptation, and persistence within mutualistic and pathogenic systems in the Vibironaceae. Biofilms offer protection against environmental stressors, immune responses, and antimicrobial treatment...

  • Review
  • Open Access
3 Citations
4,056 Views
33 Pages

Greener Approaches to Combat Biofilm’s Antimicrobial Resistance on 3D-Printed Materials: A Systematic Review

  • Khaoula Sebbar,
  • Amal El Aabedy,
  • Saad Ibnsouda Koraichi,
  • Songul Ulag,
  • Oguzhan Gunduz and
  • Soumya Elabed

28 March 2024

In recent years, Additive Manufacturing (AM), commonly referred to as 3D printing, has garnered the attention of the scientific community due to its capacity to transform ordinary and traditional items into customized materials at an affordable cost...

  • Review
  • Open Access
20 Citations
6,254 Views
48 Pages

New Methodologies as Opportunities in the Study of Bacterial Biofilms, Including Food-Related Applications

  • Francesca Coppola,
  • Florinda Fratianni,
  • Vittorio Bianco,
  • Zhe Wang,
  • Michela Pellegrini,
  • Raffaele Coppola and
  • Filomena Nazzaro

Traditional food technologies, while essential, often face limitations in sensitivity, real-time detection, and adaptability to complex biological systems such as microbial biofilms. These constraints have created a growing demand for more advanced,...

  • Review
  • Open Access
7 Citations
5,836 Views
18 Pages

Acid Rock Drainage or Not—Oxidative vs. Reductive Biofilms—A Microbial Question

  • Margarete Kalin,
  • William N. Wheeler and
  • Sören Bellenberg

7 May 2018

Measures to counteract Acid Rock Drainage (ARD) generation need to start at the mineral surface, inhibiting mineral-oxidizing, acidophilic microbes. Laboratory and long-term field tests with pyrite-containing mining wastes—where carbonaceous ph...

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