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Article

Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study

Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, Italy
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Author to whom correspondence should be addressed.
Academic Editors: Fernando Pérez-Rodriguez and Araceli Bolívar
Foods 2021, 10(7), 1484; https://doi.org/10.3390/foods10071484
Received: 28 May 2021 / Revised: 18 June 2021 / Accepted: 23 June 2021 / Published: 26 June 2021
(This article belongs to the Special Issue Listeria in Food: Prevalence and Control)
Among food-borne pathogens, Listeria monocytogenes continues to pose concerns to food business operators due to its capacity to form biofilm in processing environments. Ozone may be an eco-friendly technology to control microbial contaminations, but data concerning its effect on Listeria monocytogenes biofilm are still limited. In this study, the effect of gaseous ozone at 50 ppm on planktonic cells and biofilm of reference and food-related Listeria monocytogenes strains was evaluated. Ozone caused a reduction in microbial loads of 3.7 ± 0.4 and 3.9 ± 0.4 Log10 CFU/mL after 10 and 30 min, respectively. A complete inactivation of planktonic cells after 6 h of treatment was observed. Biofilm inhibition and eradication treatments (50 ppm, 6 h) resulted in a significant decrease of the biofilm biomass for 59% of the strains tested, whilst a slight dampening of live cell loads in the biofilm state was observed. In conclusion, gaseous ozone is not sufficient to completely counteract Listeria monocytogenes biofilm, but it may be useful as an additional tool to contrast Listeria monocytogenes free-living cells and to improve the existing sanitization procedures in food processing environments. View Full-Text
Keywords: antimicrobial; biofilm; Listeria monocytogenes; ozone; eco-friendly technology; foodborne pathogens; food processing environment; planktonic cells; food industries; food safety antimicrobial; biofilm; Listeria monocytogenes; ozone; eco-friendly technology; foodborne pathogens; food processing environment; planktonic cells; food industries; food safety
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MDPI and ACS Style

Panebianco, F.; Rubiola, S.; Chiesa, F.; Civera, T.; Di Ciccio, P.A. Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study. Foods 2021, 10, 1484. https://doi.org/10.3390/foods10071484

AMA Style

Panebianco F, Rubiola S, Chiesa F, Civera T, Di Ciccio PA. Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study. Foods. 2021; 10(7):1484. https://doi.org/10.3390/foods10071484

Chicago/Turabian Style

Panebianco, Felice, Selene Rubiola, Francesco Chiesa, Tiziana Civera, and Pierluigi A. Di Ciccio. 2021. "Effect of Gaseous Ozone on Listeria monocytogenes Planktonic Cells and Biofilm: An In Vitro Study" Foods 10, no. 7: 1484. https://doi.org/10.3390/foods10071484

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