Next Article in Journal
Design and Performance Analysis of a Supercritical Carbon Dioxide Heat Exchanger
Previous Article in Journal
An Enhanced Precoder for Multi User Multiple-Input Multiple-Output Downlink Systems
Due to scheduled maintenance work on our core network, there may be short service disruptions on this website between 16:00 and 16:30 CEST on September 25th.
Article

A Reproducible Method for Growing Biofilms on Polystyrene Surfaces: Biomass and Bacterial Viability Evolution of Pseudomonas fluorescens and Staphylococcus epidermidis

1
BioTeC+-Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, 9000 Gent, Belgium
2
Procter&Gamble, Newcastle Innovation Center, Newcastle NE12 9TS, UK
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(13), 4544; https://doi.org/10.3390/app10134544
Received: 23 April 2020 / Revised: 12 June 2020 / Accepted: 23 June 2020 / Published: 30 June 2020
(This article belongs to the Section Applied Biosciences and Bioengineering)
Since biofilm development represents a crucial issue within industrial, clinical and domestic sectors, innovative technologies/approaches (e.g., light technology for inactivation, antibiofilm coatings) are required to eradicate them. In this multidisciplinary scenario, protocols for the development of biofilms are necessary, particularly, in laboratories (not specialised in biofilm science) lacking in sophisticated devices for their growth. A protocol was developed for growing Pseudomonas fluorescens (Gram-negative) biofilms on wide, flat, polystyrene surfaces within 24 h. Several factors, such as inoculum level, area size and growth medium concentration, were investigated. Biofilm development was studied via viable cells and biomass quantification. A comparative analysis between kinetics and growth parameters, estimated using the Baranyi and Roberts model, was conducted at different inoculum levels (104 and 107 CFU/mL). The inoculum levels did not influence the final population within the 24-h-grown biofilms, but they influenced the total biomass development, which followed different kinetics. Confocal laser scanning microscopy confirmed that overnight growth allowed for development of a densely packed biofilm with its 3D structure. The developed protocol was validated for Staphylococcus epidermidis (Gram-positive). The present work is the first study to develop an easy-to-use protocol to obtain highly reproducible biofilms, on flat polystyrene surfaces, with no need for sophisticated technologies. View Full-Text
Keywords: biofilms; crystal violet; growth curve; polystyrene; flat surface; Pseudomonas fluorescens; Staphylococcus epidermidis biofilms; crystal violet; growth curve; polystyrene; flat surface; Pseudomonas fluorescens; Staphylococcus epidermidis
Show Figures

Figure 1

MDPI and ACS Style

Angarano, V.; Smet, C.; Akkermans, S.; Akritidou, T.; Huyck, B.; Chieffi, A.; Impe, J.F.M.V. A Reproducible Method for Growing Biofilms on Polystyrene Surfaces: Biomass and Bacterial Viability Evolution of Pseudomonas fluorescens and Staphylococcus epidermidis. Appl. Sci. 2020, 10, 4544. https://doi.org/10.3390/app10134544

AMA Style

Angarano V, Smet C, Akkermans S, Akritidou T, Huyck B, Chieffi A, Impe JFMV. A Reproducible Method for Growing Biofilms on Polystyrene Surfaces: Biomass and Bacterial Viability Evolution of Pseudomonas fluorescens and Staphylococcus epidermidis. Applied Sciences. 2020; 10(13):4544. https://doi.org/10.3390/app10134544

Chicago/Turabian Style

Angarano, Valeria, Cindy Smet, Simen Akkermans, Theodora Akritidou, Bart Huyck, Andre Chieffi, and Jan F.M.V. Impe 2020. "A Reproducible Method for Growing Biofilms on Polystyrene Surfaces: Biomass and Bacterial Viability Evolution of Pseudomonas fluorescens and Staphylococcus epidermidis" Applied Sciences 10, no. 13: 4544. https://doi.org/10.3390/app10134544

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop