You are currently on the new version of our website. Access the old version .

128 Results Found

  • Article
  • Open Access
6 Citations
6,800 Views
10 Pages

Microbial diversity has always presented taxonomic challenges. With the popularity of next-generation sequencing technology, more unculturable bacteria have been sequenced, facilitating the discovery of additional new species and complicated current...

  • Systematic Review
  • Open Access
4 Citations
2,130 Views
15 Pages

Gut Microbial Taxonomy and Its Role as a Biomarker in Aortic Diseases: A Systematic Review and Future Perspectives

  • Dina Neiroukh,
  • Aida Hajdarpasic,
  • Cagri Ayhan,
  • Sherif Sultan and
  • Osama Soliman

18 November 2024

Background/Objectives: Evidence of the association between the gut microbiome and cardiovascular diseases has accumulated. An imbalance or dysbiosis of this system has been shown to play a role in the pathogenesis of cardiovascular events, including...

  • Article
  • Open Access
5 Citations
3,014 Views
21 Pages

17 January 2023

Studies of ant biodiversity are important to understand their group better, as well as to extend our knowledge on the evolution of their associated organisms. Host-associated microbial communities, and particularly bacterial communities, are shaped b...

  • Article
  • Open Access
6 Citations
2,841 Views
22 Pages

Shotgun metagenomic sequencing was used to investigate the diversity of the microbial community of Cheddar cheese ripened over 32 months. The changes in taxa abundance were compared from assembly-based, non-assembly-based, and mOTUs2 sequencing pipel...

  • Article
  • Open Access
210 Citations
9,830 Views
25 Pages

The Taxon Hypothesis Paradigm—On the Unambiguous Detection and Communication of Taxa

  • Urmas Kõljalg,
  • Henrik R. Nilsson,
  • Dmitry Schigel,
  • Leho Tedersoo,
  • Karl-Henrik Larsson,
  • Tom W. May,
  • Andy F. S. Taylor,
  • Thomas Stjernegaard Jeppesen,
  • Tobias Guldberg Frøslev and
  • Kessy Abarenkov
  • + 11 authors

Here, we describe the taxon hypothesis (TH) paradigm, which covers the construction, identification, and communication of taxa as datasets. Defining taxa as datasets of individuals and their traits will make taxon identification and most importantly...

  • Article
  • Open Access
168 Views
23 Pages

Carbon (C) is crucial for nutrient cycling and the assembly of microbial populations in the soil. However, it is still unclear how the C-source utilization characteristics of microbes in distinct types of soils respond to changes in soil phosphorus (...

  • Article
  • Open Access
48 Citations
11,875 Views
11 Pages

TaxCollector: Modifying Current 16S rRNA Databases for the Rapid Classification at Six Taxonomic Levels

  • Adriana Giongo,
  • Austin G. Davis-Richardson,
  • David B. Crabb and
  • Eric W. Triplett

21 July 2010

The high level of conservation of 16S ribosomal RNA gene (16S rRNA) in all Prokaryotes makes this gene an ideal tool for the rapid identification and classification of these microorganisms. Databases such as the Ribosomal Database Project II (RDP-II)...

  • Perspective
  • Open Access
6 Citations
4,161 Views
10 Pages

On a Non-Discrete Concept of Prokaryotic Species

  • Juan M. Gonzalez,
  • Elena Puerta-Fernández,
  • Margarida M. Santana and
  • Bhagwan Rekadwad

The taxonomic concept of species has received continuous attention. A microbial species as a discrete box contains a limited number of highly similar microorganisms assigned to that taxon, following a polyphasic approach. In the 21st Century, with th...

  • Article
  • Open Access
3 Citations
2,637 Views
13 Pages

24 February 2024

Staphylococcus schleiferi and Staphylococcus coagulans, closely related bacterial species within the Staphylococcus genus, present a challenge in classification and diagnosis due to their close genetic proximity and overlapping phenotypic features. M...

  • Article
  • Open Access
2,561 Views
9 Pages

11 March 2022

A Gram-stain-negative, facultatively aerobic, pink and oval bacterium, designed OS4T, was isolated from a sediment sample taken from a coastal zone in China. The growth of OS4T occurred at 20–37 °C (optimal 25 °C), pH 7.0–8.5 (opt...

  • Article
  • Open Access
4 Citations
4,670 Views
17 Pages

Sporobolomyces lactucae sp. nov. (Pucciniomycotina, Microbotryomycetes, Sporidiobolales): An Abundant Component of Romaine Lettuce Phylloplanes

  • Samira Fatemi,
  • Danny Haelewaters,
  • Hector Urbina,
  • Samuel Brown,
  • Makenna L. Houston and
  • M. Catherine Aime

16 March 2022

Shifts in food microbiomes may impact the establishment of human pathogens, such as virulent lineages of Escherichia coli, and thus are important to investigate. Foods that are often consumed raw, such as lettuce, are particularly susceptible to such...

  • Article
  • Open Access
10 Citations
5,269 Views
15 Pages

Spatial Metagenomic Analysis in Understanding the Microbial Diversity of Thar Desert

  • Jagdish Parihar,
  • Suraj P. Parihar,
  • Prashanth Suravajhala and
  • Ashima Bagaria

17 March 2022

The arid and semi-arid regions of Rajasthan are one of the most extreme biomes of India, possessing diverse microbial communities that exhibit immense biotechnological potential for industries. Herein, we sampled study sites from arid and semi-arid r...

  • Article
  • Open Access
26 Citations
6,918 Views
11 Pages

The taxonomic composition of microbial communities can be assessed using universal marker amplicon sequencing. The most common taxonomic markers are the 16S rDNA for bacterial communities and the internal transcribed spacer (ITS) region for fungal co...

  • Article
  • Open Access
10 Citations
2,455 Views
13 Pages

16 February 2023

Background: This study sought to characterize the saliva microbiota of Candida carriage Sjögren’s syndrome (SS) patients compared to oral candidiasis and healthy patients by high-throughput sequencing. Methods: Fifteen patients were includ...

  • Article
  • Open Access
123 Citations
25,770 Views
8 Pages

Microbial Community Analysis of Sauerkraut Fermentation Reveals a Stable and Rapidly Established Community

  • Michelle A. Zabat,
  • William H. Sano,
  • Jenna I. Wurster,
  • Damien J. Cabral and
  • Peter Belenky

12 May 2018

Despite recent interest in microbial communities of fermented foods, there has been little inquiry into the bacterial community dynamics of sauerkraut, one of the world’s oldest and most prevalent fermented foods. In this study, we utilize 16S...

  • Communication
  • Open Access
1,725 Views
7 Pages

CPGminer: An Interactive Dashboard to Explore the Genomic Features and Taxonomy of Complete Prokaryotic Genomes

  • Jaehyun Kim,
  • Sunghyun Yoon,
  • Sandeep Kondakala,
  • Steven L. Foley,
  • Mark Hart,
  • Dong-Heon Baek,
  • Wenjun Wang,
  • Sung-Kwan Kim,
  • John B. Sutherland and
  • Ohgew Kweon
  • + 1 author

Prokaryotes, the earliest forms of life on Earth, play crucial roles in global biogeochemical processes in virtually all ecosystems. The ever-increasing amount of prokaryotic genome sequencing data provides a wealth of information to examine fundamen...

  • Systematic Review
  • Open Access
18 Citations
3,893 Views
23 Pages

The Tissue-Associated Microbiota in Colorectal Cancer: A Systematic Review

  • Carolina Pinto da Costa,
  • Patrícia Vieira,
  • Melissa Mendes-Rocha,
  • Joana Pereira-Marques,
  • Rui Manuel Ferreira and
  • Ceu Figueiredo

12 July 2022

The intestinal microbiome is associated with colorectal cancer. Although the mucosal microbiota better represents an individual’s local microbiome, studies on the colorectal cancer microbiota mainly reflect knowledge obtained from fecal samples...

  • Article
  • Open Access
15 Citations
5,386 Views
14 Pages

Informative Regions In Viral Genomes

  • Jaime Leonardo Moreno-Gallego and
  • Alejandro Reyes

18 June 2021

Viruses, far from being just parasites affecting hosts’ fitness, are major players in any microbial ecosystem. In spite of their broad abundance, viruses, in particular bacteriophages, remain largely unknown since only about 20% of sequences obtained...

  • Article
  • Open Access
8 Citations
2,860 Views
13 Pages

pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale

  • Ying Xu,
  • Jianwei Wang,
  • Qingjie Liu,
  • Qun Zhang,
  • Jiazhong Wu,
  • Minghui Zhou,
  • Yong Nie and
  • Xiao-Lei Wu

Oil reservoirs are one of the most important deep subsurface biospheres. They are inhabited by diverse microorganisms including bacteria and archaea with diverse metabolic activities. Although recent studies have investigated the microbial communitie...

  • Article
  • Open Access
1 Citations
2,011 Views
17 Pages

This study introduces a groundbreaking approach for the exploration and utilization of electrotrophic acetogens, essential for advancing microbial electrosynthesis systems (MES). Our initial focus was the development of Solid-Phase Electrochemical Is...

  • Article
  • Open Access
2,735 Views
13 Pages

Trait-Based Method of Quantitative Assessment of Ecological Functional Groups in the Human Intestinal Microbiome

  • Andrew I. Kropochev,
  • Sergey A. Lashin,
  • Yury G. Matushkin and
  • Alexandra I. Klimenko

11 January 2023

We propose the trait-based method for quantifying the activity of functional groups in the human gut microbiome based on metatranscriptomic data. It allows one to assess structural changes in the microbial community comprised of the following functio...

  • Article
  • Open Access
1 Citations
3,189 Views
22 Pages

Industrial wastewaters display a complex and diverse range of physicochemical properties that are measured, studied, and treated by businesses and water service providers. Less frequently measured are the microbial communities in these wastes, despit...

  • Feature Paper
  • Article
  • Open Access
11 Citations
4,324 Views
16 Pages

28 August 2020

Advanced onsite wastewater treatment systems (OWTS) use biological nitrogen removal (BNR) to mitigate the threat that N-rich wastewater poses to coastal waterbodies and groundwater. These systems lower the N concentration of effluent via sequential m...

  • Article
  • Open Access
4 Citations
2,460 Views
18 Pages

Exploring Gut Microbiome Composition and Circulating Microbial DNA Fragments in Patients with Stage II/III Colorectal Cancer: A Comprehensive Analysis

  • Ippokratis Messaritakis,
  • Andreas Koulouris,
  • Eleni Boukla,
  • Konstantinos Vogiatzoglou,
  • Ilias Lagkouvardos,
  • Evangelia Intze,
  • Maria Sfakianaki,
  • Maria Chondrozoumaki,
  • Michaela Karagianni and
  • John Souglakos
  • + 10 authors

18 May 2024

Background: Colorectal cancer (CRC) significantly contributes to cancer-related mortality, necessitating the exploration of prognostic factors beyond TNM staging. This study investigates the composition of the gut microbiome and microbial DNA fragmen...

  • Article
  • Open Access
9 Citations
2,841 Views
14 Pages

A Multivariate Approach to Study the Bacterial Diversity Associated to the Wooden Shelves Used for Aging Traditional Sicilian Cheeses

  • Raimondo Gaglio,
  • Gabriele Busetta,
  • Riccardo Gannuscio,
  • Luca Settanni,
  • Giuseppe Licitra and
  • Massimo Todaro

7 March 2022

The present study was carried to correlate the microbial diversity of the biofilms developed on the wooden boards used for aging traditional Sicilian cheeses with cheese typology. To this end, the microbial diversity of the shelves in contact with th...

  • Article
  • Open Access
3 Citations
2,995 Views
15 Pages

4 September 2021

This study examined the correlation between non-metallic organic tanning agents (NOTAs) and wastewater containing double-triazine (bistriazine). Specifically, high-throughput sequencing was used to analyze how the physical and chemical properties of...

  • Review
  • Open Access
2 Citations
4,703 Views
17 Pages

Challenges in the Identification of Environmental Bacterial Isolates from a Pharmaceutical Industry Facility by 16S rRNA Gene Sequences

  • Juliana Nunes Ramos,
  • Luciana Veloso da Costa,
  • Verônica Viana Vieira and
  • Marcelo Luiz Lima Brandão

7 July 2025

Microbial contamination is a critical challenge for the pharmaceutical industry, especially in thermosensitive sterile products, and can compromise their quality and safety. The accurate identification of microorganisms is essential to trace sources...

  • Review
  • Open Access
20 Citations
10,285 Views
24 Pages

Optimizing the Gut Microbiota for Individualized Performance Development in Elite Athletes

  • Svenja Nolte,
  • Karsten Krüger,
  • Claudia Lenz and
  • Karen Zentgraf

5 December 2023

The human gut microbiota can be compared to a fingerprint due to its uniqueness, hosting trillions of living organisms. Taking a sport-centric perspective, the gut microbiota might represent a physiological system that relates to health aspects as we...

  • Article
  • Open Access
6 Citations
4,421 Views
10 Pages

Measuring Dietary Botanical Diversity as a Proxy for Phytochemical Exposure

  • Henry J. Thompson,
  • Jack O. Levitt,
  • John N. McGinley,
  • Paulette Chandler,
  • Patricia M. Guenther,
  • Inge Huybrechts and
  • Mary C. Playdon

14 April 2021

The study of natural plant molecules and their medicinal properties, pharmacognosy, provides a taxonomy for botanical families that represent diverse chemical groupings with potentially distinct functions in relation to human health. Yet, this reserv...

  • Article
  • Open Access
3 Citations
1,785 Views
31 Pages

This study evaluates the potential of biorefinery and dairy wastewater as substrates for electricity generation in double chamber Microbial Fuel Cells (DCMFC), focusing on their microbial taxonomy and electrochemical viability. Taxonomic analysis usi...

  • Article
  • Open Access
56 Citations
44,466 Views
20 Pages

Differential Effects of Western and Mediterranean-Type Diets on Gut Microbiota: A Metagenomics and Metabolomics Approach

  • Claudia Barber,
  • Marianela Mego,
  • Carlos Sabater,
  • Fernando Vallejo,
  • Rogger Alvaro Bendezu,
  • Marcela Masihy,
  • Francisco Guarner,
  • Juan Carlos Espín,
  • Abelardo Margolles and
  • Fernando Azpiroz

30 July 2021

Our aim was to determine the effect of diet on gut microbiota, digestive function and sensations, using an integrated clinical, metagenomics and metabolomics approach. We conducted a cross-over, randomised study on the effects of a Western-type diet...

  • Article
  • Open Access
7 Citations
3,832 Views
14 Pages

Taxonomic Binning Approaches and Functional Characteristics of the Microbial Community during the Anaerobic Digestion of Hydrolyzed Corncob

  • Luz Breton-Deval,
  • Ilse Salinas-Peralta,
  • Jaime Santiago Alarcón Aguirre,
  • Belkis Sulbarán-Rangel and
  • Kelly Joel Gurubel Tun

24 December 2020

Maize forms the basis of Mexican food. As a result, approximately six million tons of corncob are produced each year, which represents an environmental issue, as well as a potential feedstock for biogas production. This research aimed to analyze the...

  • Article
  • Open Access
12 Citations
5,891 Views
13 Pages

Spatial Variations of Bacterial Communities of an Anaerobic Lagoon-Type Biodigester Fed with Dairy Manure

  • Marleny García-Lozano,
  • Inty Omar Hernández-De Lira,
  • David H. Huber and
  • Nagamani Balagurusamy

1 July 2019

Anaerobic digestion technology is being widely employed for sustainable management of organic wastes generated in animal farms, industries, etc. Nevertheless, biodigester microbiome is still considered a “black box” because it is regulate...

  • Article
  • Open Access
3 Citations
6,019 Views
15 Pages

Temporal Comparison of Microbial Community Structure in an Australian Winery

  • Cristian Varela,
  • Kathleen Cuijvers and
  • Anthony Borneman

Most modern fermented foods and beverages are produced in fit-for-purpose facilities which are designed to ensure not only a reliable product, but also one safe for consumption. Despite careful hygiene, microorganisms can colonise these facilities an...

  • Article
  • Open Access
2 Citations
2,551 Views
17 Pages

11 March 2023

(1) Background: Microbial conversion of gaseous molecules, such as CO2, CO and H2, to valuable compounds, has come to the forefront since the beginning of the 21st century due to increasing environmental concerns and the necessity to develop alternat...

  • Article
  • Open Access
10 Citations
2,788 Views
13 Pages

Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing

  • Fen Hou,
  • Junjie Du,
  • Ye Yuan,
  • Xihui Wu and
  • Sai Zhao

7 April 2021

Aged refuse is widely considered to have certain soil fertility. 16S rRNA amplicon sequencing is used to investigate the microbial community of aged refuse. The aged refuse is found to contain higher soil fertility elements (total nitrogen, total pho...

  • Article
  • Open Access
1,814 Views
13 Pages

A Protocol for the Characterization of Diatom Communities in Mountain Glaciers

  • Susana Chamorro,
  • María Borrego-Ramos,
  • Carlos Silva,
  • Dayana Estrada,
  • Sara López,
  • José Salazar,
  • Jennifer Moyón,
  • Eloy Becares and
  • Saúl Blanco

27 November 2024

This research presents a significant contribution to the methodologies and protocols for studying diatom communities in cryoconite holes on glaciers. Cryoconite holes are unique microenvironments found on glacial surfaces that support intricate micro...

  • Review
  • Open Access
3 Citations
2,488 Views
12 Pages

From Species to Genes: A New Diagnostic Paradigm

  • Sinead Fahy,
  • James A. O’Connor,
  • Roy D. Sleator and
  • Brigid Lucey

Molecular diagnostics has the potential to revolutionise the field of clinical microbiology. Microbial identification and nomenclature have, for too long, been restricted to phenotypic characterisation. However, this species-level view fails to wholl...

  • Article
  • Open Access
59 Citations
6,758 Views
17 Pages

Soil Microbial Diversity and Community Composition in Rice–Fish Co-Culture and Rice Monoculture Farming System

  • Noppol Arunrat,
  • Chakriya Sansupa,
  • Praeploy Kongsurakan,
  • Sukanya Sereenonchai and
  • Ryusuke Hatano

20 August 2022

Soil microorganisms play an important role in determining nutrient cycling. The integration of fish into rice fields can influence the diversity and structural composition of soil microbial communities. However, regarding the rice–fish co-cultu...

  • Article
  • Open Access
16 Citations
2,987 Views
13 Pages

Seasonal Changes in the Distinct Taxonomy and Function of the Gut Microbiota in the Wild Ground Squirrel (Spermophilus dauricus)

  • Xiaoying Yang,
  • Yuchen Yao,
  • Xueying Zhang,
  • Jiahui Zhong,
  • Fuli Gao,
  • Haolin Zhang,
  • Yingying Han,
  • Qiang Weng and
  • Zhengrong Yuan

13 September 2021

Seasonal breeding is a normal phenomenon in which animals adapt to natural selection and reproduce only in specific seasons. Large studies have reported that the gut microbiota is closely related to reproduction. The purpose of this study was to expl...

  • Article
  • Open Access
10 Citations
3,604 Views
16 Pages

Exploring Longitudinal Gut Microbiome towards Metabolic Functional Changes Associated in Atopic Dermatitis in Early Childhood

  • Preecha Patumcharoenpol,
  • Amornthep Kingkaw,
  • Massalin Nakphaichit,
  • Pantipa Chatchatee,
  • Narissara Suratannon,
  • Gianni Panagiotou and
  • Wanwipa Vongsangnak

20 September 2023

Atopic dermatitis (AD) is a prevalent inflammatory skin disease that has been associated with changes in gut microbial composition in early life. However, there are limited longitudinal studies examining the gut microbiome in AD. This study aimed to...

  • Article
  • Open Access
1 Citations
1,484 Views
15 Pages

Metagenomic Insights into Microbial Signatures in Thrombi from Acute Ischemic Stroke Patients Undergoing Endovascular Treatment

  • Kasthuri Thirupathi,
  • Sherief Ghozy,
  • Abdullah Reda,
  • Wasantha K. Ranatunga,
  • Mars A. Ruben,
  • Zarrintan Armin,
  • Oana M. Mereuta,
  • Sekhon Prabhjot,
  • Daying Dai and
  • Ramanathan Kadirvel
  • + 2 authors

6 February 2025

Background: Variability in recanalization success during endovascular treatment for acute ischemic stroke (AIS) has led to increased interests in thrombus composition and associated cellular materials. While evidence suggests that bacteria may influe...

  • Article
  • Open Access
8 Citations
3,279 Views
19 Pages

13 December 2022

Moss-dominated biocrusts are widespread in degraded mining ecosystems and play an important role in soil development and ecosystem primary succession. In this work, the soil microbial community structure under moss-dominated biocrusts in ionic rare e...

  • Article
  • Open Access
37 Citations
4,976 Views
21 Pages

Demonstration of Allium sativum Extract Inhibitory Effect on Biodeteriogenic Microbial Strain Growth, Biofilm Development, and Enzymatic and Organic Acid Production

  • Viorica Maria Corbu,
  • Irina Gheorghe,
  • Ioana Cristina Marinaș,
  • Elisabeta Irina Geană,
  • Maria Iasmina Moza,
  • Ortansa Csutak and
  • Mariana Carmen Chifiriuc

27 November 2021

To the best of our knowledge, this is the first study demonstrating the efficiency of Allium sativum hydro-alcoholic extract (ASE) againstFigure growth, biofilm development, and soluble factor production of more than 200 biodeteriogenic microbial str...

  • Article
  • Open Access
25 Citations
4,407 Views
12 Pages

Exploring the Association between Gut and Urine Microbiota and Prostatic Disease including Benign Prostatic Hyperplasia and Prostate Cancer Using 16S rRNA Sequencing

  • Kai-Yen Tsai,
  • Deng-Chyang Wu,
  • Wen-Jeng Wu,
  • Jiunn-Wei Wang,
  • Yung-Shun Juan,
  • Ching-Chia Li,
  • Chung-Jung Liu and
  • Hsiang-Ying Lee

Numerous microorganisms residing in the gastrointestinal and genitourinary tracts affect host health. We investigated stool and voided urine samples collected from patients with benign prostatic hyperplasia (BPH) or prostate cancer (PC) and a control...

  • Article
  • Open Access
6 Citations
1,863 Views
24 Pages

Stochasticity Highlights the Development of Both the Gastrointestinal and Upper-Respiratory-Tract Microbiomes of Neonatal Dairy Calves in Early Life

  • A. Nathan Frazier,
  • Logan Ferree,
  • Aeriel D. Belk,
  • Khalid Al-Lakhen,
  • M. Caitlin Cramer and
  • Jessica L. Metcalf

27 January 2025

The microbiome of dairy calves undergoes extensive change due to various forces during the first weeks of life. Importantly, diseases such as bovine respiratory disease (BRD) and calf diarrhea can have profound impacts on the early-life microbiome. T...

  • Article
  • Open Access
2 Citations
2,840 Views
11 Pages

8 January 2022

The division of hard and soft feces is an effective digestion strategy in the order Lagomorpha. Although previous studies have reported that hard and soft feces differ in morphology and component, the discrepancy in the microbiome remains unclear. Th...

  • Review
  • Open Access
23 Citations
1,540 Views
16 Pages

Endofungal bacteria are bacterial symbionts of fungi that exist within fungal hyphae and spores. There is increasing evidence that these bacteria, alone or in combination with their fungal hosts play a critical role in tripartite symbioses with plant...

  • Article
  • Open Access
13 Citations
5,056 Views
27 Pages

Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

  • Tamara Nazina,
  • Tamara Babich,
  • Nadezhda Kostryukova,
  • Diyana Sokolova,
  • Ruslan Abdullin,
  • Tatyana Tourova,
  • Vitaly Kadnikov,
  • Andrey Mardanov,
  • Nikolai Ravin and
  • Kenji Kato
  • + 6 authors

8 February 2020

The goal of the present work was to investigate the physicochemical and radiochemical conditions and the microbial diversity in groundwater collected near the Lake Karachai (Russia), which was formerly used for the disposal of liquid radioactive wast...

of 3