Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Soil Sample Acquisition
2.2. Physical and Chemical Indicator Measurements
2.3. DNA Extraction and High-Throughput Sequencing
2.4. High-Throughput Sequencing Data Analysis
2.5. Statistical Analysis
3. Results
3.1. Comparison of Physical and Chemical Factors
3.2. Microbial Community Structure Composition
3.3. Diversity of the Microbial Community
3.4. The Interactions between the Microorganisms and Physical or Chemical Indicators
3.5. The Predictive Function Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
Abbreviations
MSW | municipal solid waste |
SOC | soil organic carbon |
TK | total potassium |
TP | total phosphorus |
TN | total nitrogen |
Hg | mercury |
As | arsenic |
Pb | lead |
Cr | chromium |
Ni | nickel |
Cd | cadmium |
Zn | zinc |
CSPSA | control standards of pollutants in sludge for agricultural use |
SCAL | soil contamination risk of agricultural land |
Appendix A
Metal (mg/kg) | Aged Refuse | Control | CSPSA Class A Upper Limit | CSPSAU Class B Upper Limit | SCAL Upper Limit |
---|---|---|---|---|---|
Pb | 38.48 ± 4.1 | 16.92 ± 0.92 | 300 | 1000 | 170 |
Cr | 46.36 ± 4.53 | 65.89 ± 4.32 | 500 | 1000 | 250 |
Ni | 29.12 ± 3.28 | 32.19 ± 2.01 | 100 | 200 | 190 |
Cd | 0.42 ± 0.06 | 0.21 ± 0.01 | 3 | 15 | 0.6 |
Zn | 163.83 ± 9.67 | 58.67 ± 1.81 | 1200 | 3000 | 300 |
As | 8.45 ± 0.33 | 13.18 ± 0.38 | 30 | 75 | 25 |
Hg | 0.15 ± 0.02 | 0.06 ± 0.01 | 3 | 15 | 3.4 |
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Samples | Simpson | Chao1 | ACE | Shannon |
---|---|---|---|---|
T1 | 0.993826 | 3118.17 | 3207.86 | 9.33 |
T2 | 0.996125 | 3321.49 | 3708.06 | 9.69 |
T3 | 0.995019 | 3411.14 | 3652.43 | 9.59 |
CK1 | 0.998292 | 3049 | 3049 | 10.73 |
CK2 | 0.997839 | 3000 | 3000 | 10.56 |
CK3 | 0.997648 | 3349.22 | 3359.57 | 10.7 |
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Hou, F.; Du, J.; Yuan, Y.; Wu, X.; Zhao, S. Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing. Sustainability 2021, 13, 4111. https://doi.org/10.3390/su13084111
Hou F, Du J, Yuan Y, Wu X, Zhao S. Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing. Sustainability. 2021; 13(8):4111. https://doi.org/10.3390/su13084111
Chicago/Turabian StyleHou, Fen, Junjie Du, Ye Yuan, Xihui Wu, and Sai Zhao. 2021. "Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing" Sustainability 13, no. 8: 4111. https://doi.org/10.3390/su13084111