Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules...
Understanding the mechanisms of hydrocarbon migration, accumulation, and alteration, particularly how evolution controls these processes, is critical for exploring lithologic hydrocarbons in reservoirs. In the complex tectonic settings of the contine...
The Central Depression of the Junggar Basin relies heavily on Permian lacustrine mudstone for deep-seated hydrocarbon sealing. This research investigated how the fluorescence parameters of caprock samples responded to the leakage of palaeo-oil zones...
Fluorescence, the absorption and re-emission of photons with longer wavelengths, is one of those amazing phenomena of Nature. Its discovery and utilization had, and still has, a major impact on biological and biomedical research, since it enables res...
Three-dimensional (3D) printing technology holds great potential to fabricate complex constructs in the field of regenerative medicine. Researchers in the surgical fields have used 3D printing techniques and their associated biomaterials for educatio...
Lipid rafts are dynamic microdomains in the membrane, rich in cholesterol and sphingolipids, that are critical for biological processes like cell signalling, membrane trafficking, and protein organization. Their essential role is claimed in both phys...
Distinguishing composite remnants from tooth structure after trauma splint removal can be challenging. This study aimed to compare the Fluorescence-aided Identification Technique (FIT) with conventional light illumination (CONV) in terms of accuracy...
Atmospheric peroxides, especially hydrogen peroxide (H2O2), are essential oxidants. The peroxide concentration is closely related to the extent of OH radicals and the O3 cycle in the tropospheric atmospheric chemistry. However, only a few studies hav...
In order to clarify the local environment during interpolymer complex formation between poly(carboxylic acids) and poly(acrylamide derivatives) with different N-substitutions, a fluorescence label technique was used. 3-(2-propenyl)-9-(4-N,N-dimethyla...
In the past decade(s), fluorescence microscopy and laser scanning confocal microscopy (LSCM) have been widely employed to investigate biological and biomimetic systems for pharmaceutical applications, to determine the localization of drugs in tissues...
Diffuse low-grade gliomas are infiltrative tumors whose margins are not distinguishable from the adjacent healthy brain parenchyma. The aim was to precisely examine the results provided by the intraoperative use of macroscopic fluorescence in diffuse...
F resonance energy transfer (FRET) occurs when the distance between a donor fluorophore and an acceptor is within 10 nm, and its application often necessitates fluorescent labeling of biological targets. However, covalent modification of biomolecules...
Colorectal liver metastases (CRLM) afflict a significant proportion of patients with colorectal cancer (CRC), ranging from 25% to 30% of patients throughout the course of the disease. In recent years, there has been a surge of interest in the applica...
This comprehensive review aims to explore the applications of indocyanine green (ICG) in robot-assisted urological surgery through a detailed examination of fluorescence-guided techniques. An extensive literature search was conducted in PubMed/MEDLIN...
Protein amyloid aggregation has been associated with more than 50 human disorders, including the most common neurodegenerative disorders Alzheimer’s and Parkinson’s disease. Interfering with this process is considered as a promising thera...
We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques...
The pharmaceutical industry has witnessed exponential growth in production volumes, driven by factors such as an aging global population and the COVID-19 pandemic. To meet the demand for high product quality alongside increased productivity, there is...
Citrus diseases are significant threats to citrus groves, causing financial losses through reduced fruit size, blemishes, premature fruit drop, and tree death. The detection of citrus diseases via leaf inspection can improve grove management and miti...
Rose Bengal (RB) is a fluorescent dye with several potential biomedical applications, particularly in dermatology. Due to RB’s poor physicochemical properties, several advanced delivery systems have been developed as a potential tool to promote...
Dual or multimodal imaging probes have emerged as powerful tools that improve detection sensitivity and accuracy in disease diagnosis by imaging techniques. Two imaging techniques that are complementary and do not use ionizing radiation are magnetic...
Imaging methods based on visible luminescence induced by ultraviolet (UV) radiation are well consolidated in the investigation of ancient works of art, to map varnishes, retouches, and possibly some pigments. As far as contemporary art is involved, t...
The provenance study of archaeological materials is an important step in understanding the cultural and economic life of ancient human communities. One of the most popular approaches in provenance studies is to obtain the chemical composition of mate...
The paper presents four new sites where bright green Ulva thalli were found inhabiting freshwater (a river, a ditch, the Milet Canal) and marine (on the rocky shore of the Adriatic Sea) habitats in Montenegro. The aims of this study were to determine...
X-ray fluorescence (XRF) spectrometry has proven to be a core, non-destructive, analytical technique in cultural heritage studies mainly because of its non-invasive character and ability to rapidly reveal the elemental composition of the analyzed art...
This study evaluates the potential of quantum dots (QDs) as a marking method for Mediterranean fruit flies (Ceratitis capitata) (Medfly) in comparison to traditional fluorescent powder. As a highly destructive pest impacting a wide variety of fruit c...
Fatliquoring is one of the most important processes in leather making, in which the collagen fibers are split effectively, while the crust acquires a good softness and mechanical strength. The effectiveness of fatliquoring depends not only on the var...
With the rapid scientific and technological changes that occur every day, a new kind of necessity, real-time, rapid, and accurate detection methods, preferably also non- or minimally invasive and non-destructive, has emerged. One such method is laser...
In the implementation of mosquito control strategy programs using Sterile Insect Technique and other rear and release strategies, knowledge on the dispersion, competitiveness and survival of mosquitos is considered essential. To assess these paramete...
Flow Mediated Skin Fluorescence (FMSF) is a new non-invasive method for assessing vascular circulation and/or metabolic regulation. It enables assessment of both vasoconstriction and vasodilation. The method measures stimulation of the circulation in...
The requirements for early diagnostics as well as effective treatment of insidious diseases such as cancer constantly increase the pressure on development of efficient and reliable methods for targeted drug/gene delivery as well as imaging of the tre...
This study proposes a method for estimating the spectral images of fluorescence spectral distributions emitted from plant grains and leaves without using a spectrometer. We construct two types of multiband imaging systems with six channels, using ord...
Genetically-encoded fluorescent sensors have been actively developed over the last few decades and used in live imaging and drug screening. Real-time monitoring of drug action in a specific cellular compartment, organ, or tissue type; the ability to...
The interaction between plasmonic nanoparticles and organic dye molecules plays an important role in varied photonic and optoelectronic applications. In this work, we systematically investigate the optical properties of a water-soluble perylenediimid...
The fluorescence decays (FDs) of 27 dried vegetative bacteria, bacterial endospores, fungi, and pollens were measured and determined using a stroboscopic technique. Pulsed nanosecond LED sources, emitting light at wavelengths of 280, 340, and 460 nm,...
This research explores the use of the pepN activity fluorescent sensor DCM-Ala in bacterial biofilms, emphasizing its significance due to the critical role of biofilms in various biological processes. Advanced imaging techniques were employed to visu...
Several strategies, including UV detection with a diode array detector (DAD), laser-induced fluorescence (LIF), derivatization reactions, the use of micelles in the separation buffer, as well as online preconcentration techniques based on pressure-as...
Anthracyclines are a group of antineoplastic compounds used to treat acute leukemia and other cancers, and they are excreted after consumption by patients. These chemicals are often found in sewage at very low concentration levels. For this reason, t...
Monitoring the biological fate of medicaments within the environments of cancer cells is an important challenge which is nowadays the object of intensive studies. In this regard, rhodamine-based supramolecular systems are one of the most suitable pro...
Vulnerable atherosclerotic carotid plaques are prone to rupture, resulting in ischemic strokes. In contrast to radiological imaging techniques, molecular imaging techniques have the potential to assess plaque vulnerability by visualizing diseases-spe...
This work provides a synthesis of an initial experience in the development of accessible imaging techniques and their implementation on a real case: the analysis of colonial Hispano-American paintings at the Complejo Museográfico Provincial &l...
Casein glycomacropeptide (GMP) is known to promote the in vitro growth of Bifidobacteria and Lactobacilli. In this paper, we used conventional culture techniques and fluorescent in situ hybridization (FISH) techniques to investigate the effect of cas...
This paper presents the application of three non-destructive techniques in the study of an agricultural area on the west coast of Peloponnese, Greece. The applied methods include (a) electromagnetic geophysical research using a handheld EM profiler (...
The detection of biological agents using optical systems is an open field of research. Currently, different spectroscopic techniques allow to detect and classify chemical agents while a fast and accurate technique able to identify biological agents i...
Analytical devices for bacterial detection are an integral part of modern laboratory medicine, as they permit the early diagnosis of diseases and their timely treatment. Therefore, special attention is directed to the development of and improvements...
The exhibition “The Ladies of Art”, held at the Palazzo Reale in Milan in 2021, focused on the history of women artists during the 16th and 17th centuries. As part of the exhibition, a series of diagnostic analyses have been carried out o...
The advancement of super-resolution imaging (SRI) relies on fluorescent proteins with novel photochromic properties. Using light, the reversibly switchable fluorescent proteins (RSFPs) can be converted between bright and dark states for many photocyc...
The effect of the chemical structure of selected phenolic acids on the molecular organization of gliadins was investigated with the application of Fourier Transform Infrared (FTIR) technique, steady-state, and time-resolved fluorescence spectroscopy....
This study presents the results of the technical investigation carried out on several English portrait miniatures painted in the 16th and 17th century by Nicholas Hilliard and Isaac Oliver, two of the most famous limners working at the Tudor and Stua...
Maximal safe surgical resection remains a critical component of glioblastoma (GBM) management, improving both survival and quality of life. However, complete tumor removal is hindered by the infiltrative nature of GBM and its proximity to eloquent br...
The ‘Tomb of the Philosophers’, owing its name to the iconographic theme depicted on the interior of its walls, offers a rare example of high-quality early Hellenistic painting, which enhances our knowledge on the use of painting material...
of 72