The goal of this study was to develop the
Listeria species-specific PCR assays based on a house-keeping gene (
lmo1634) encoding alcohol acetaldehyde
dehydrogenase (Aad), previously designated as
Listeria adhesion protein (LAP), and compare results with a label-free light scattering sensor, BARDOT
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The goal of this study was to develop the
Listeria species-specific PCR assays based on a house-keeping gene (
lmo1634) encoding alcohol acetaldehyde
dehydrogenase (Aad), previously designated as
Listeria adhesion protein (LAP), and compare results with a label-free light scattering sensor, BARDOT (bacterial rapid detection using optical scattering technology). PCR primer sets targeting the
lap genes from the species of
Listeria sensu stricto were designed and tested with 47
Listeria and 8 non-
Listeria strains. The resulting PCR primer sets detected either all species
of
Listeria sensu stricto or individual
L. innocua,
L. ivanovii and
L. seeligeri,
L. welshimeri, and
L. marthii without producing any amplified products from other bacteria tested. The PCR assays with
Listeria sensu stricto-specific primers also successfully detected all species of
Listeria sensu stricto and/or
Listeria innocua from mixed culture-inoculated food samples, and each bacterium in food was verified by using the light scattering sensor that generated unique scatter signature for each species of
Listeria tested. The PCR assays based on the house-keeping gene
aad (
lap) can be used for detection of either all species of
Listeria sensu stricto or certain individual
Listeria species in a mixture from food with a detection limit of about 10
4 CFU/mL.
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