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Open AccessArticle

An Apta-Biosensor for Colon Cancer Diagnostics

Department of Nanobiotechnology, School of New Sciences and Technologies, University of Tehran, Tehran 14395-1561, Iran
Department of Biotechnology and Pharmacy Engineering, Faculty of Chemical Engineering, University of Tehran, Tehran 4563-11155, Iran
Research Center of New Technologies in Life Science Engineering, University of Tehran, Tehran 1417963891, Iran
Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran 1419963111, Iran
Department of Electrical Engineering and Computer Science, York University, Toronto, ON M3J1P3, Canada
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Sensors 2015, 15(9), 22291-22303;
Received: 18 May 2015 / Revised: 19 August 2015 / Accepted: 26 August 2015 / Published: 3 September 2015
(This article belongs to the Special Issue Label-Free Sensing)
This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics. View Full-Text
Keywords: aptasensor; aptamer; cyclic voltammetry (CV); colon cancer; biosensor; fluorescence labeling aptasensor; aptamer; cyclic voltammetry (CV); colon cancer; biosensor; fluorescence labeling
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Raji, M.A.; Amoabediny, G.; Tajik, P.; Hosseini, M.; Ghafar-Zadeh, E. An Apta-Biosensor for Colon Cancer Diagnostics. Sensors 2015, 15, 22291-22303.

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