7,181 Results Found

Cloning and expression plasmids are the workhorses of modern molecular biology. Despite the pathway paved by synthetic biology, laboratories around the globe still relay on standard cloning techniques using plasmids with reporter proteins for positiv...

To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are requ...

Over 40 years ago, scientists imagined ways cloning could aid conservation of threatened taxa. The cloning of Dolly the sheep from adult somatic cells in 1996 was the breakthrough that finally enabled the conservation potential of the technology. Unt...

Appropriate reliance on code clones significantly reduces development costs and hastens the development process. Reckless cloning, in contrast, reduces code quality and ultimately adds costs and time. To avoid this scenario, many researchers have pro...

Software clones may cause vulnerability proliferation, which highlights the importance of investigating clone-incurred vulnerabilities. In this paper, we propose a framework for automatically managing clone-incurred vulnerabilities. Two innovations o...

Lymphocyte activation gene 3 (LAG3) is a T cell inhibitory receptor that promotes tumor cell immune escape and is a potential target for cancer diagnostic and immunotherapeutic applications. We used automated capillary electrophoresis (ACE), surface...

Software clones are code fragments with similar or nearly similar functionality or structures. These clones are introduced in a project either accidentally or deliberately during software development or maintenance process. The presence of clones pos...

For many years, it was of interest to identify the sequences encoding the two melatonin receptors (MT1 and MT2) from various species. After publishing the basic molecular characterization of the human, rat, mouse, sheep, and platypus MT1, MT2, or Mel...

Code clone detection is an important research topic in the field of software engineering. It is significant in developing software and solving software infringement disputes to discover code clone phenomenon effectively in and between software system...

To increase public understanding in cloned animals produced by somatic cell nuclear transfer technology, our previous study investigated the carcass trait and meat quality of the clones (paper accepted), and this study we further evaluate differences...

With progress in quantum technologies, the field of quantum networks has emerged as an important area of research. In the last few years, there has been substantial progress in understanding the correlations present in quantum networks. In this artic...

Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics stud...

With the rapid development of the internet of things, radio frequency identification (RFID) technology plays an important role in various fields. However, RFID systems are vulnerable to cloning attacks. This is the fabrication of one or more replicas...

Major histocompatibility complex class I (MHC-I) gene expression in the placenta is modulated to tailor the maternal immune response to fetal antigens during pregnancy. This study evaluated MHC-I expression through immunohistochemistry (IHC) using an...

In this research, in vitro culture was applied to evaluate five alfalfa lines for salt tolerance and to select and multiply the most resistant clones. The pregerminated seeds were tested on a semisolid culture medium enriched with four different NaCl...

Code cloning is a common practice in software development, where developers reuse existing code to accelerate programming speed and enhance work efficiency. Existing clone-detection methods mainly focus on code clones within a single programming lang...

The vulnerability of the speaker identity verification system to attacks using voice cloning was examined. The research project assumed creating a model for verifying the speaker’s identity based on voice biometrics and then testing its resista...

The paper presents some forestry aspects of using Inger and Tordis willow clones to obtain woody biomass and remedy degraded soils. The methodological aspects regarding the planting of willow seedlings, the evaluation of the survival rate, the evalua...

The physical and chemical properties of poplar clones largely determine their suitability for different applications. The main objective of this study was to investigate clonal variation in four hybrid poplar clones grown at three sites in North Chin...

Infectious cloning of plant viruses is a powerful tool for studying the reverse genetic manipulation of viral genes in virus–host plant interactions, contributing to a deeper understanding of the life history and pathogenesis of viruses. Yet, m...

Poplar (Populus) clones are widely used for riparian afforestation owing to their fast growth and ecological benefits. However, selecting suitable clones for site-specific conditions remains a key challenge. In this study, we evaluated the survival a...

Improving productivity to reduce the cost of biologics manufacturing and ensure that therapeutics can reach more patients remains a major challenge faced by the biopharmaceutical industry. Chinese hamster ovary (CHO) cell lines are commonly prepared...

The technique of cloning has wide applications in animal husbandry and human biomedicine. However, the very low developmental efficiency of cloned embryos limits the application of cloning. Ectopic XIST-expression-induced abnormal X chromosome inacti...

To select excellent clones characterized by fast growth and high-quality wood of Catalpa fargesii Bur., growth traits, including height and diameter at breast height (DBH) at 1, 9, 11 and 13 years old, were collected and wood properties, including th...

IAN873, Dongfang93114 and Reyan73397, created through vegetative propagation for their high yield and excellent cold resistance, are major clones planted in China. In this work, latexes with rubber particles of the same size from these clones are sep...

Campylobacter jejuni clone SA has emerged as the predominant cause of ovine abortion outbreaks in the United States (US). Despite the fact that commercial Campylobacter vaccines are available, their efficacy in protecting abortion induced by C. jejun...

We apply the data cloning method to estimate a medium-scale dynamic stochastic general equilibrium model. The data cloning algorithm is a numerical method that employs replicas of the original sample to approximate the maximum likelihood estimator as...

Cytoplasm injection cloning technology (CICT) is an efficient technique for evaluating the developmental potential of cloned embryos. In this study, we investigated the effects of donor cell type on the developmental potential and quality of cloned b...

Begomovirus has become a potential threat to the agriculture sector. It causes significant losses to several economically important crops. Given this considerable loss, the development of tools to study viral genomes and function is needed. Infectiou...

Honey bees (Apis mellifera) play a crucial role in agriculture through their pollination activities. However, they have faced significant health challenges over the past decades that can limit colony performance and even lead to collapse. A primary c...

The development of the wireless sensor networks technology commonly named WSNs has been gaining a significantly increased amount of attention from researchers over the last few decades. Its large number of sensor nodes is one of the features that mak...

In recent years, plantations of Aquilaria sinensis in China have been dominated by Qi-nan, yet there remains limited research on the growth evaluation and breeding of these clones. In this study, a multi-point joint variance analysis, an additive mai...

Multiresistant Escherichia coli has been disseminated worldwide, and it is one of the major causative agents of nosocomial infections. E. coli has a remarkable and complex genomic plasticity for taking up and accumulating genetic elements; thus, mult...

This paper addresses the problem of IoT security caused by code cloning when developing a massive variety of different smart devices. A clone detection method is proposed to identify clone-caused vulnerabilities in IoT software. A hybrid solution com...

A forward genetic approach is a powerful tool for identifying the genes underlying the phenotypes of interest. However, the conventional map-based cloning method is lengthy, requires a large mapping population and confirmation of many candidate genes...

Source code clones are common in software development as part of reuse practice. However, they are also often a source of errors compromising software maintainability. The existing work on code clone detection mainly focuses on clones in a single pro...

Somatic embryogenesis (SE) cloning techniques for Nordmann fir Christmas tree production have been pursued and refined in Denmark, and three SE clonal trials with 201 SE clones from seeds of 27 Nordmann fir trees and seedlings from bulk harvest seeds...

Decades of antimicrobial overuse to treat respiratory disease in foals have promoted the emergence and spread of zoonotic multidrug-resistant (MDR) Rhodococcus equi worldwide. Three main R. equi MDR clonal populations—2287, G2106, and G2017&mda...

Abnormalities in animals cloned via somatic cell nuclear transfer (SCNT) have been reported. In this study, to produce bomb-sniffing dogs, we successfully cloned four healthy dogs through SCNT using the same donor genome from the skin of a male Germa...

The technique of pig cloning holds great promise for the livestock industry, life science, and biomedicine. However, the prenatal death rate of cloned pig embryos is extremely high, resulting in a very low cloning efficiency. This limits the developm...

Radio Frequency Identification (RFID) technology is widely applied in various scenarios, including logistics tracking, supply chain management, and target monitoring. In these contexts, the malicious cloning of legitimate tag information can lead to...

Cloning from historically cryopreserved cells offers a potential means to restore lost genetic variation or increase the representation of particular lineages within bottlenecked species, provided such biobanked materials are archived for such geneti...

Internet of things (IoT) technologies have recently gained much interest from numerous industries, where devices, machines, sensors, or simply things are linked with each other over open communication networks. However, such an operation environment...

Tea clones and seed varieties released from Tocklai Tea Research Institutes are suitable primarily for manufacturing black tea (CTC/orthodox) while green tea manufactured from these clones are poor in quality. This led to identifying germplasm suitab...

Natural rubber from different Hevea braziliensis clones, namely RRIM600, RRIT251, PB235 and BPM24, exhibit unique properties. The influences of the various fresh natural rubber latex and cream concentrated latex on the non-rubber components related p...

Algae are attractive organisms for biotechnology applications such as the production of biofuels, medicines, and other high-value compounds due to their genetic diversity, varied physical characteristics, and metabolic processes. As new species are b...

Clones of Catalpa bungei and Catalpa fargesii f. duclouxii were studied over several years in central China to explore genetic variation in growth traits and to identify clones of high wood yield and high stability. The genetic parameters for height,...

Expression cloning from cDNA is an important technique for acquiring genes encoding novel fluorescent proteins. However, the probability of in-frame cDNA insertion following the first start codon of the vector is normally only 1/3, which is a cause o...

As Large Language Models (LLMs) continue to advance, their capabilities in code clone detection have garnered significant attention. While much research has assessed LLM performance on human-generated code, the proliferation of LLM-generated code rai...

Willows are suitable candidates for phytoremediation projects. A pot experiment was conducted to evaluate the potential of using Salix unrooted cuttings for the phytoremediation of lead/zinc (Pb/Zn) and copper (Cu) mine tailings. Cuttings of 14 Salix...