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243 Results Found

  • Review
  • Open Access
42 Citations
9,025 Views
18 Pages

Molecular Mechanism and Agricultural Application of the NifA–NifL System for Nitrogen Fixation

  • Wenyao Zhang,
  • Yihang Chen,
  • Keyang Huang,
  • Feng Wang and
  • Ziqing Mei

Nitrogen–fixing bacteria execute biological nitrogen fixation through nitrogenase, converting inert dinitrogen (N2) in the atmosphere into bioavailable nitrogen. Elaborating the molecular mechanisms of orderly and efficient biological nitrogen...

  • Article
  • Open Access
26 Citations
6,599 Views
21 Pages

Comparative Genomics Provides Insights into the Taxonomy of Azoarcus and Reveals Separate Origins of Nif Genes in the Proposed Azoarcus and Aromatoleum Genera

  • Roberto Tadeu Raittz,
  • Camilla Reginatto De Pierri,
  • Marta Maluk,
  • Marcelo Bueno Batista,
  • Manuel Carmona,
  • Madan Junghare,
  • Helisson Faoro,
  • Leonardo M. Cruz,
  • Federico Battistoni and
  • Euan K. James
  • + 5 authors

7 January 2021

Among other attributes, the Betaproteobacterial genus Azoarcus has biotechnological importance for plant growth-promotion and remediation of petroleum waste-polluted water and soils. It comprises at least two phylogenetically distinct groups. The &ld...

  • Article
  • Open Access
7 Citations
2,415 Views
22 Pages

Secure NIfTI Image Authentication Scheme for Modern Healthcare System

  • Kamred Udham Singh,
  • Turki Aljrees,
  • Ankit Kumar and
  • Teekam Singh

24 April 2023

Advances in digital neuroimaging technologies, i.e., MRI and CT scan technology, have radically changed illness diagnosis in the global healthcare system. Digital imaging technologies produce NIfTI images after scanning the patient’s body. COVI...

  • Article
  • Open Access
13 Citations
9,661 Views
12 Pages

9 January 2013

Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertio...

  • Article
  • Open Access
5 Citations
4,448 Views
13 Pages

Sweet potato cultivars obtained from different nursery farmers were cultivated in an experimental field from seedling-stage to harvest, and the acetylene reduction activity (ARA) of different parts of the plant as well as the nifH genes associated wi...

  • Article
  • Open Access
12 Citations
4,868 Views
13 Pages

Paenibacillus is a genus of Gram-positive, facultative anaerobic and endospore-forming bacteria. Genomic sequence analysis has revealed that a compact nif (nitrogen fixation) gene cluster comprising 9–10 genes nifBHDKENX(orf1)hesAnifV is conser...

  • Article
  • Open Access
895 Views
16 Pages

31 March 2025

(1) Background: Rhizobia can promote plant growth by providing essential nutrients such as NH4+ and PO43−; thus, rhizobia that can tolerate the stress of heavy metals will be conducive to the phytoremediation of heavy-metal-contaminated soils....

  • Article
  • Open Access
18 Citations
5,537 Views
15 Pages

6 December 2018

The nematicidal activity of the common plant-pathogenic bacterium Pseudomonas syringae against certain nematodes has been recently identified, but little is known about its virulence factors. In the current study, predictive analysis of nematode-viru...

  • Perspective
  • Open Access
10 Citations
4,668 Views
22 Pages

13 November 2020

The family of nitrogenase enzymes catalyzes the reduction of atmospheric dinitrogen (N2) to ammonia under remarkably benign conditions of temperature, pressure, and pH. Therefore, the development of synthetic complexes or materials that can similarly...

  • Article
  • Open Access
54 Citations
11,517 Views
21 Pages

The phylogeny of nitrogenase has only been analyzed using the structural proteins NifHDK. As nifHDKENB has been established as the minimum number of genes necessary for in silico prediction of diazotrophy, we present an updated phylogeny of diazotrop...

  • Article
  • Open Access
41 Citations
9,333 Views
19 Pages

Metabolomic Profiling of Bradyrhizobium diazoefficiens-Induced Root Nodules Reveals Both Host Plant-Specific and Developmental Signatures

  • Martina Lardi,
  • Valérie Murset,
  • Hans-Martin Fischer,
  • Socorro Mesa,
  • Christian H. Ahrens,
  • Nicola Zamboni and
  • Gabriella Pessi

Bradyrhizobium diazoefficiens is a nitrogen-fixing endosymbiont, which can grow inside root-nodule cells of the agriculturally important soybean and other host plants. Our previous studies described B. diazoefficiens host-specific global expression c...

  • Article
  • Open Access
28 Citations
6,231 Views
20 Pages

Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris

  • Martina Lardi,
  • Yilei Liu,
  • Gabriela Purtschert,
  • Samanta Bolzan de Campos and
  • Gabriella Pessi

15 December 2017

Paraburkholderia phymatum belongs to the β-subclass of proteobacteria. It has recently been shown to be able to nodulate and fix nitrogen in symbiosis with several mimosoid and papilionoid legumes. In contrast to the symbiosis of legumes with α-prote...

  • Article
  • Open Access
5 Citations
2,778 Views
14 Pages

Characteristics of Microbial Abundance in Rhizosphere and Non-Rhizosphere Soils of Permafrost Peatland, Northeast China

  • Chao Gong,
  • Xiuyan Ma,
  • Yanyu Song,
  • Dan Zhang,
  • Mengyuan Zhu,
  • Xianwei Wang,
  • Siqi Gao,
  • Jinli Gao and
  • Changchun Song

3 September 2023

The rhizosphere microenvironment is crucial to plant–soil physiological processes. The differences among microbial communities in the rhizosphere and non-rhizosphere peatland topsoil (0–15 cm) and subsoil (15–30 cm) in five plant co...

  • Review
  • Open Access
33 Citations
8,905 Views
15 Pages

Cytomegalovirus (CMV) Pneumonitis: Cell Tropism, Inflammation, and Immunity

  • Luís Fonseca Brito,
  • Wolfram Brune and
  • Felix R. Stahl

Human cytomegalovirus (HCMV) is an opportunistic pathogen causing disease mainly in immunocompromised patients or after congenital infection. HCMV infection of the respiratory tract leads to pneumonitis in the immunocompromised host, which is often a...

  • Article
  • Open Access
1 Citations
2,198 Views
12 Pages

Insights into Genetic and Physiological Characteristics of Clover Rhizobia in Afghanistan Soils

  • Safiullah Habibi,
  • Michiko Yasuda,
  • Shafiqullah Aryan,
  • Tadashi Yokoyama,
  • Kalimullah Saighani and
  • Naoko Ohkama-Ohtsu

Livestock production in Afghanistan highly relies on grazing and clover feed, which is a key component of pastures and forage crops. This study elucidated the genetic diversity of clover-nodulating rhizobia in different ecological regions and their e...

  • Article
  • Open Access
1 Citations
1,580 Views
18 Pages

Spatial and Seasonal Analysis of Phyllosphere Bacterial Communities of the Epiphytic Gymnosperm Zamia pseudoparasitica

  • Lilisbeth Rodríguez-Castro,
  • Adriel M. Sierra,
  • Juan Carlos Villarreal Aguilar and
  • Kristin Saltonstall

Phyllosphere microbial communities influence the growth and productivity of plants, particularly in epiphytic plants, which are disconnected from nutrients available in the soil. We characterized the phyllosphere of 30 individuals of the epiphytic cy...

  • Article
  • Open Access
3 Citations
3,442 Views
10 Pages

First-Principle Investigation of Hypothetical NiF4 Crystal Structures

  • Tilen Lindič,
  • Anthony Schulz and
  • Beate Paulus

15 November 2022

An important synthetic route for the fluorinated organic compounds is electrochemical fluorination (ECF). This is a process taking place on a nickel anode immersed in anhydrous HF. Even though the mechanism is not fully resolved, it is believed that...

  • Article
  • Open Access
11 Citations
5,116 Views
10 Pages

Purple Sulfur Bacteria Dominate Microbial Community in Brazilian Limestone Cave

  • Eric L. S. Marques,
  • João C. T. Dias,
  • Eduardo Gross,
  • Adriana B. de Cerqueira e Silva,
  • Suzana R. de Moura and
  • Rachel P. Rezende

The mineralogical composition of caves makes the environment ideal for inhabitation by microbes. However, the bacterial diversity in the cave ecosystem remains largely unexplored. In this paper, we described the bacterial community in an oxic chamber...

  • Article
  • Open Access
62 Citations
5,014 Views
26 Pages

Unraveling Nitrogen Fixing Potential of Endophytic Diazotrophs of Different Saccharum Species for Sustainable Sugarcane Growth

  • Rajesh Kumar Singh,
  • Pratiksha Singh,
  • Anjney Sharma,
  • Dao-Jun Guo,
  • Sudhir K. Upadhyay,
  • Qi-Qi Song,
  • Krishan K. Verma,
  • Dong-Ping Li,
  • Mukesh Kumar Malviya and
  • Yang-Rui Li
  • + 2 authors

Sugarcane (Saccharum officinarum L.) is one of the world’s highly significant commercial crops. The amounts of synthetic nitrogen (N2) fertilizer required to grow the sugarcane plant at its initial growth stages are higher, which increases the...

  • Article
  • Open Access
3 Citations
2,487 Views
16 Pages

Biochar and Flooding Increase and Change the Diazotroph Communities in Tropical Paddy Fields

  • Yunzhong Chen,
  • Yan Zhao,
  • Yuqin Wang,
  • Xinrui Liu,
  • Xiaofeng Qin,
  • Qiqi Chen,
  • Qilin Zhu,
  • Ahmed S. Elrys,
  • Yanzheng Wu and
  • Lei Meng

Biological nitrogen fixation (BNF) can reduce synthetic N fertilizer application and improve N-use efficiency. However, knowledge about the effect of biochar and water management regimes on soil diazotrophic microorganisms in tropical paddy fields re...

  • Article
  • Open Access
6 Citations
3,079 Views
6 Pages

Improvement of the NIFS Atom and Molecular Database

  • Masahiko Emoto,
  • Izumi Murakami,
  • Daiji Kato,
  • Masanobu Yoshida,
  • Masatoshi Kato and
  • Setsuo Imazu

11 September 2019

The NIFS (National Institute for Fusion Science) Atom and Molecular Database, which has been available online since 1997, is a numerical atomic and molecular database of collision processes that is important for fusion research. This database provide...

  • Article
  • Open Access
7 Citations
4,069 Views
22 Pages

Metabolomics and Dual RNA-Sequencing on Root Nodules Revealed New Cellular Functions Controlled by Paraburkholderia phymatum NifA

  • Paula Bellés-Sancho,
  • Martina Lardi,
  • Yilei Liu,
  • Leo Eberl,
  • Nicola Zamboni,
  • Aurélien Bailly and
  • Gabriella Pessi

Paraburkholderia phymatum STM815 is a nitrogen-fixing endosymbiont that nodulate the agriculturally important Phaseolus vulgaris and several other host plants. We previously showed that the nodules induced by a STM815 mutant of the gene encoding the...

  • Article
  • Open Access
33 Citations
5,811 Views
16 Pages

Nitrogen-Fixing Bacteria Associated with Peltigera Cyanolichens and Cladonia Chlorolichens

  • Katerin Almendras,
  • Jaime García,
  • Margarita Carú and
  • Julieta Orlando

25 November 2018

Lichens have been extensively studied and described; however, recent evidence suggests that members of the bacterial community associated with them could contribute new functions to the symbiotic interaction. In this work, we compare the nitrogen-fix...

  • Article
  • Open Access
3 Citations
3,000 Views
16 Pages

11 August 2022

The sweet potato (Ipomoea batatas (L.) Lam.) is an important and widely grown crop, and the nitrogenase reductase (nifH) gene is the most widely sequenced marker gene used to identify nitrogen-fixing bacteria and archaea. There have been many example...

  • Article
  • Open Access
16 Citations
3,864 Views
13 Pages

Composition and Activity of N2-Fixing Microorganisms in Mangrove Forest Soils

  • Yo-Jin Shiau,
  • Yu-Te Lin,
  • Rita S. W. Yam,
  • Ed-Haun Chang,
  • Jhe-Ming Wu,
  • Tsu-Hsuan Hsu and
  • Chih-Yu Chiu

22 June 2021

Mangrove forests are considered to be a highly productive ecosystem, but they are also generally nitrogen (N)-limited. Thus, soil N2 fixation can be important for the stability of both mangrove ecosystem functions and upland N supply. This study eval...

  • Article
  • Open Access
29 Citations
6,903 Views
16 Pages

Plant growth promoting endophytic bacteria, which can fix nitrogen, plays a vital role in plant growth promotion. Previous authors have evaluated the effect of Gluconacetobacter diazotrophicus Pal5 inoculation on plants subjected to different sources...

  • Article
  • Open Access
33 Citations
6,369 Views
32 Pages

Key Aroma Compounds of Dark Chocolates Differing in Organoleptic Properties: A GC-O Comparative Study

  • Zoé Deuscher,
  • Karine Gourrat,
  • Marie Repoux,
  • Renaud Boulanger,
  • Hélène Labouré and
  • Jean-Luc Le Quéré

15 April 2020

Dark chocolate samples were previously classified into four sensory categories. The classification was modelled based on volatile compounds analyzed by direct introduction mass spectrometry of the chocolates’ headspace. The purpose of the study...

  • Review
  • Open Access
6 Citations
5,023 Views
13 Pages

16 August 2021

Eukaryotic organisms such as plants are unable to utilise nitrogen gas (N2) directly as a source of this essential element and are dependent either on its biological conversion to ammonium by diazotrophic prokaryotes, or its supply as chemically synt...

  • Article
  • Open Access
5 Citations
3,600 Views
20 Pages

Total and diazotrophic bacteria were assessed in the rhizosphere soils of native and encroaching legumes growing in the Succulent Karoo Biome (SKB), South Africa. These were Calobota sericea, Lessertia diffusa, Vachellia karroo, and Wiborgia monopter...

  • Review
  • Open Access
13 Citations
3,335 Views
18 Pages

Lupin, a Unique Legume That Is Nodulated by Multiple Microsymbionts: The Role of Horizontal Gene Transfer

  • Abdelhakim Msaddak,
  • Mohamed Mars,
  • Miguel A. Quiñones,
  • M. Mercedes Lucas and
  • José J. Pueyo

Lupin is a high-protein legume crop that grows in a wide range of edaphoclimatic conditions where other crops are not viable. Its unique seed nutrient profile can promote health benefits, and it has been proposed as a phytoremediation plant. Most rhi...

  • Article
  • Open Access
9 Citations
3,002 Views
16 Pages

19 January 2022

Degumming is the most important link in the textile industry. The main purpose of degumming is to effectively remove non-cellulose substances in plant bast fibers. In this research, we propose an electro-Fenton (EF) system with a nickel-foam (Ni-F) c...

  • Article
  • Open Access
1 Citations
1,081 Views
18 Pages

Screening and Validation of Rhizobial Strains for Improved Lentil Growth

  • Tianda Chang,
  • Tao Yang,
  • Meng Ren,
  • Xinghui Li,
  • Xuerui Fang,
  • Bingjie Niu,
  • Hongbin Yang,
  • Lixiang Wang and
  • Ximing Chen

Lentil is a nutritionally valuable legume crop, rich in protein, carbohydrates, amino acids, and vitamins, and is also used as green manure. Symbiotic nitrogen fixation (SNF) plays a crucial role in lentil growth and development, yet there is limited...

  • Article
  • Open Access
13 Citations
4,497 Views
20 Pages

Paraburkholderia phymatum Homocitrate Synthase NifV Plays a Key Role for Nitrogenase Activity during Symbiosis with Papilionoids and in Free-Living Growth Conditions

  • Paula Bellés-Sancho,
  • Martina Lardi,
  • Yilei Liu,
  • Sebastian Hug,
  • Marta Adriana Pinto-Carbó,
  • Nicola Zamboni and
  • Gabriella Pessi

20 April 2021

Homocitrate is an essential component of the iron-molybdenum cofactor of nitrogenase, the bacterial enzyme that catalyzes the reduction of dinitrogen (N2) to ammonia. In nitrogen-fixing and nodulating alpha-rhizobia, homocitrate is usually provided t...

  • Article
  • Open Access
2 Citations
1,953 Views
14 Pages

27 April 2024

Electrochemical fluorination on nickel anodes, also known as the Simons’ process, is an important fluorination method used on an industrial scale. Despite its success, the mechanism is still under debate. One of the proposed mechanisms involves...

  • Article
  • Open Access
2 Citations
1,335 Views
21 Pages

The Phytoremediation of Arsenic-Contaminated Waste by Poa labillardieri, Juncus pauciflorus, and Rytidosperma caespitosum

  • Feizia Huslina,
  • Leadin S. Khudur,
  • Julie A. Besedin,
  • Kamrun Nahar,
  • Kalpit Shah,
  • Aravind Surapaneni,
  • Pacian Netherway and
  • Andrew S. Ball

Phytoremediation represents a potentially effective and environmentally friendly technology to remediate arsenic (As) in mine waste soils. However, soil amendments are often required to improve phytoremediation due to depleted nutrients in mine waste...

  • Article
  • Open Access
14 Citations
5,307 Views
11 Pages

Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture

  • Jyoti A. Mohite,
  • Kumal Khatri,
  • Kajal Pardhi,
  • Shubha S. Manvi,
  • Rutuja Jadhav,
  • Shilpa Rathod and
  • Monali C. Rahalkar

27 September 2023

Rice fields are one of the important anthropogenic sources of methane emissions. Methanotrophs dwelling near the rice roots and at the oxic–anoxic interface of paddy fields can oxidize a large fraction of the generated methane and are therefore...

  • Article
  • Open Access
9 Citations
4,708 Views
12 Pages

NIFS Atomic and Molecular Numerical Database for Collision Processes

  • Izumi Murakami,
  • Masatoshi Kato,
  • Masahiko Emoto,
  • Daiji Kato,
  • Hiroyuki A. Sakaue and
  • Tomoko Kawate

9 October 2020

The National Institute for Fusion Science (NIFS) has compiled and developed atomic and molecular numerical databases for various collision processes and makes it accessible from the internet to the public. The database contains numerical data of cros...

  • Review
  • Open Access
38 Citations
10,327 Views
14 Pages

The Fe Protein: An Unsung Hero of Nitrogenase

  • Andrew J. Jasniewski,
  • Nathaniel S. Sickerman,
  • Yilin Hu and
  • Markus W. Ribbe

Although the nitrogen-fixing enzyme nitrogenase critically requires both a reductase component (Fe protein) and a catalytic component, considerably more work has focused on the latter species. Properties of the catalytic component, which contains two...

  • Article
  • Open Access
15 Citations
4,173 Views
14 Pages

NifS and NifU (encoded by nifS and nifU) are generally dedicated to biogenesis of the nitrogenase Fe–S cluster in diazotrophs. However, nifS and nifU are not found in N2-fixing Paenibacillus strains, and the mechanisms involved in Fe–S cluster biosyn...

  • Article
  • Open Access
3 Citations
1,569 Views
18 Pages

Phytobacter palmae WL65, isolated from the rice rhizosphere, was confirmed as P. palmae through whole-genome analysis. WL65 exhibited key plant growth-promoting (PGP) characteristics, including nitrogen fixation (nifA, nifB, nifD, nifE, nifF, nifH, n...

  • Article
  • Open Access
16 Citations
2,759 Views
10 Pages

Plasmonic Gold Nanoisland Film for Bacterial Theranostics

  • Shih-Hua Tan,
  • Sibidou Yougbaré,
  • Hsuan-Ya Tao,
  • Che-Chang Chang and
  • Tsung-Rong Kuo

21 November 2021

Plasmonic nanomaterials have been intensively explored for applications in biomedical detection and therapy for human sustainability. Herein, plasmonic gold nanoisland (NI) film (AuNIF) was fabricated onto a glass substrate by a facile seed-mediated...

  • Article
  • Open Access
4 Citations
2,159 Views
14 Pages

17 April 2024

Plasmonic metal nanomaterials have been extensively investigated for their utilizations in biomedical sensing and treatment. In this study, plasmonic Au@Ag core–shell nanoisland films (Au@AgNIFs) were successfully grown onto a glass substrate u...

  • Communication
  • Open Access
1,346 Views
9 Pages

Non-symbiotic diazotrophic microbes are important contributors to global N budgets in cereal crops. Knowledge of the biogeography of the organisms in this functional guild increases our understanding of biological N fixation in diverse locations and...

  • Article
  • Open Access
8 Citations
4,093 Views
13 Pages

26 May 2021

Non-indigenous fish species (NIFS) can cause severe ecological impacts on the invaded ecosystems and are considered as one of the leading factors of freshwater biodiversity loss. Unraveling the spatial overlap between NIFS and critically endangered (...

  • Article
  • Open Access
4 Citations
1,969 Views
12 Pages

Nitrogen-Fixing Paenibacillus haidiansis and Paenibacillus sanfengchensis: Two Novel Species from Plant Rhizospheres

  • Weilong Zhang,
  • Miao Gao,
  • Rui Hu,
  • Yimin Shang,
  • Minzhi Liu,
  • Peichun Lan,
  • Shuo Jiao,
  • Gehong Wei and
  • Sanfeng Chen

Two strains, M1 and H32 with nitrogen-fixing ability, were isolated from the rhizospheres of different plants. Genome sequence analysis showed that a nif (nitrogen fixation) gene cluster composed of nine genes (nifB nifH nifD nifK nifE nifN nifX hesA...

  • Article
  • Open Access
6 Citations
2,269 Views
11 Pages

Our objective is to develop a robust method to quantify valsartan (VAL) and nifedipine (NIF) in developed a self-nanoemulsifying drug delivery formulation. An in-house ultra-high-performance liquid chromatography (UHPLC UV) method has been developed...

  • Article
  • Open Access
9 Citations
3,607 Views
12 Pages

30 June 2021

Thermoleptolyngbya is a genus of non-heterocystous cyanobacteria that are typical inhabitants of hot spring microbial mats. These filamentous cyanobacteria are capable of nitrogen fixation. In this study, we examined the genome sequences of five publ...

  • Review
  • Open Access
4 Citations
4,682 Views
13 Pages

The availability of fixed nitrogen limits overall agricultural crop production worldwide. The so-called modern “green revolution” catalyzed by the widespread application of nitrogenous fertilizer has propelled global population growth. It...

  • Article
  • Open Access
5 Citations
2,779 Views
16 Pages

Memory CD8 T Cells Protect against Cytomegalovirus Disease by Formation of Nodular Inflammatory Foci Preventing Intra-Tissue Virus Spread

  • Rafaela Holtappels,
  • Jürgen Podlech,
  • Kirsten Freitag,
  • Niels A. Lemmermann and
  • Matthias J. Reddehase

25 May 2022

Cytomegaloviruses (CMVs) are controlled by innate and adaptive immune responses in an immunocompetent host while causing multiple organ diseases in an immunocompromised host. A risk group of high clinical relevance comprises transiently immunocomprom...

  • Review
  • Open Access
39 Citations
12,698 Views
38 Pages

Mechanism of Iron–Sulfur Cluster Assembly: In the Intimacy of Iron and Sulfur Encounter

  • Batoul Srour,
  • Sylvain Gervason,
  • Beata Monfort and
  • Benoit D’Autréaux

Iron–sulfur (Fe–S) clusters are protein cofactors of a multitude of enzymes performing essential biological functions. Specialized multi-protein machineries present in all types of organisms support their biosynthesis. These machineries e...

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