Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture
Abstract
:1. Introduction
2. Materials and Methods
2.1. Methanotrophs Used and Genome Analysis for the Detection of Plant Growth Promotion Genes/Pathways
2.2. Pot Experiments Using Methanotrophs as Bioinoculants
2.2.1. Trial with Pure Cultures of Type I and Type II Methanotrophs
2.2.2. Trial with Mixed Cultures
3. Results and Discussion
3.1. Confirmation of Nitrogen Fixation Potential in Methanotrophs
3.2. Effect of Methanotrophic Bioinoculant on Rice Plant Growth
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name of the Organism | Representative Strain | Genome NCBI Number | Identification Using pmoA Gene | Identification Using 16S rRNA Gene | ||||
---|---|---|---|---|---|---|---|---|
Strain Name | GeneBank Accession Number | Nearest Match (with Type Strain) | % Similarity | Nearest Match (with Type Strain) | % Similarity | |||
pmoA Gene | 16S rRNA Gene | |||||||
Methylocucumis oryzae | BM10 | MT366581 | MN462841 | LAJX01 * | Methylococcaceae bacterium Sn10-6 | 100 | Methylococcaceae bacterium Sn10-6 | 100 |
Ca.Methylobacter coli | BlB1 | MH424899.2 | JADMKV01 | JADMKV01 | Methylobacter marinus A45 | 97.57 | Methylobacter marinus A45 | 98.5 |
Methylocystis spp. | Sn-Cys | KT156638.1 | MZ562889.1 | JAERVJ01 | Methylocystis iwaonis SS37A-Re | 99.34 | Methylocystis iwaonis SS37A-Re | 99.93 |
Methylomonas spp. | Kb3 | KP862532 | KM995837 | PIZT01 | Methylomonas denitrificans FJG1 | 95.72 | Methylomonas denitrificans strain FJG1 | 99.13 |
Methylomonas spp. | WWC4 | MH806338.1 | MH64454.1 | JAATWI01 | Methylomonas methanica S1 | 93 | Methylomonas koyamae FwR12E-Y | 97 |
Ca. Methylobacter oryzae | KRF1 | MH806336.1 | MK511847.1 | RYFG02 | Methylobacter tundripaludum SV96 | 90 | Methylobacter tundripaludum SV96 | 98.6 |
Methylolobus aquaticus | FWC3 | MH806335.1 | MH7895511 | SEYW01 | Methylocaldum marinum S8 | 85.9 | Methylocaldum marinum S8 | 94 |
Methylosinus sporium | KRF6 | WP_216281891.1 | MZ562999.1 | JAHLJF0.1 | Methylosinus sporium NCIMB 11126 | 91 | Methylosinus sporium NCIMB 11126 | 98.43 |
Sr. No. | Strain Name | Mean Plant Height (cm) | % Increase in Plant Height Compared to Control | Flowering Stage (Day after Sowing) | Total Yield (Weight of the Grains (g)) | % Increase in Total Yield Compared to Control |
---|---|---|---|---|---|---|
1 | Ca. Methylobacter coli strain BlB1 | 88 ± 3 | 12 | 110 | 22 | 38 |
2 | Methylocucumis oryzae strain BM10 | 88 ± 3 | 12 | 97 | 21 | 31 |
3 | Methylomagnum ishizawai strain KRF4 | 91 ± 3 | 16 | 106 | 18 | 13 |
4 | Methylomonas sp. Kb3 | 87 ± 7 | 12 | 111 | 18 | 13 |
5 | Methylosinus trichosporium strain KRF10 | 85 ± 7 | 12 | 107 | 17 | 6 |
6 | Methylomonas sp. WWC4 | 85 ± 7 | 12 | 107 | 17 | 6 |
7 | Ca. Methylobacter oryzae strain KRF1 | 84 ± 3 | 8 | 111 | 17 | 6 |
8 | Methylolobus aquaticus strain FWC3 | 83 ± 7 | 8 | 111 | 16 | - |
9 | Methylosinus sporium strain KRF6 | 77 ± 4 | - | 107 | 13 | - |
10 | Methylocystis sp. Sn-Cys | 88 ± 1 | 12 | 107 | 15 | - |
11 | Control pots 2021 (mean) | 78 ± 3 | - | 113 | 16 | - |
12 | Type I Methylomonas consortia | 78 ± 2 | 13 | 114 | 23 | 35 |
13 | Type II Methylosinus-Methylocystis consortia | 75 ± 2 | 9 | 114 | 19 | 12 |
14 | Control pots 2022 (mean) | 69 ± 2 | - | 113 | 17 | - |
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Mohite, J.A.; Khatri, K.; Pardhi, K.; Manvi, S.S.; Jadhav, R.; Rathod, S.; Rahalkar, M.C. Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture. Methane 2023, 2, 361-371. https://doi.org/10.3390/methane2040024
Mohite JA, Khatri K, Pardhi K, Manvi SS, Jadhav R, Rathod S, Rahalkar MC. Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture. Methane. 2023; 2(4):361-371. https://doi.org/10.3390/methane2040024
Chicago/Turabian StyleMohite, Jyoti A., Kumal Khatri, Kajal Pardhi, Shubha S. Manvi, Rutuja Jadhav, Shilpa Rathod, and Monali C. Rahalkar. 2023. "Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture" Methane 2, no. 4: 361-371. https://doi.org/10.3390/methane2040024