Methane

Wetlands are the most important natural sources of methane. Studies on the distribution and diversity of methanotrophs, especially in tropical wetlands, are limited. The studies on wetland methanotrophs help bridge the gap in the literature for understanding the community structure of methanotrophs in tropical wetlands. Our present study documents the methanotroph diversity from various wetland habitats across Western India. Samples from various sites, such as freshwater ponds, lake sediments, mangroves, etc., located in Western India, were collected and enriched for methanotroph isolation. An established protocol for the isolation of methanotrophs from Indian rice fields, involving serial dilution and long-term incubations, was slightly modified and used. Obtaining entirely pure cultures of methanotrophs is a labor-intensive and technically challenging process. Hence, for primary level characterization, ‘methanotroph monocultures’, which have a single methanotroph culture with minimal contamination, were established. Twenty monocultures and eight pure cultures of methanotrophs were obtained in this study. The pmoA gene has been used for the phylogenetic characterization of methanotrophs for the last 25 years. Monocultures were from seven genera: the Methylomonas, Methylocystis, Methylosinus, Methylocaldum, Methylocucumis, Methylomagnum, and Methylolobus genera. Eight pure cultures were obtained, which were strains of Methylomonas koyamae, Methylosinus sporium, and Methylolobus aquaticus. A maximum number of cultures belonged to the Type I genus Methylomonas and to the Type II genus Methylocystis. Thus, the cultivation-based community studies of methanotrophs from wetland habitats in India expanded the current knowledge about the methanotroph diversity in such regions. Additionally, the cultivation approach helped us obtain new methanotrophs from this previously unexplored habitat, which can be used for further biotechnological and environmental applications. The isolated monocultures can either be used as MMCs (mixed methanotroph consortia) for environmental applications or further purified and used as pure cultures. Full article

This work examined the effects of secondary annular flow area on flame stability in an experimental diffusion flame burner. The burner was composed of a horizontally mounted, rectangular chamber that utilized a retractable spark plug for ignition and an inverse coaxial injector. The primary and secondary gaseous reactants were oxygen and methane, respectively. Three injectors were assessed to have a fixed primary flow area and secondary flow impingement angle of 30 degrees with the primary flow and distinct secondary annular flow areas. Resultant flames and flame standoff distances were recorded via optical windows aligned parallel to the burner axis. Flame stability regime maps were generated based on the reactant equivalence ratio, the methane Reynolds number, and the injector secondary annular flow area. Results showed that among the injectors, the greater the secondary annular flow area with an impingement angle, the better the likelihood of generating a stable, anchored, fuel-rich diffusion flame for hydrogen production over the largest range of Reynolds numbers. As the secondary flow area decreased, stable diffusion flames transitioned from existing at highly turbulent flows to experiencing near-blowoff or no ignition under the same conditions. Secondary annular flow area significantly influences the location and range of stable, anchored methane/oxygen diffusion flames. Full article

Quantifying methane (CH₄) emissions is essential for climate change mitigation; however, current estimation methods often suffer from substantial uncertainties, particularly at the site level. This study introduces a drone-based approach for measuring CH₄ emissions using an open-path Tunable Diode Laser Absorption Spectroscopy (TDLAS) sensor mounted parallel to the ground, rather than in the traditional nadir-pointing configuration. Controlled CH₄ release experiments were conducted to evaluate the method’s accuracy, employing a modified mass-balance technique to estimate emission rates. Two wind data processing strategies were compared: a logarithmic wind profile (LW) and a constant scalar wind speed (SW). The LW approach yielded highly accurate results, with an average recovery rate of 98%, while the SW approach showed greater variability with increasing distance from the source, although it remained reliable in close proximity. The method demonstrated the ability to quantify emissions as low as 0.08 g s⁻¹ with approximately 4% error, given sufficient sampling. These findings suggest that the proposed UAV-based system is a promising, cost-effective tool for accurate CH₄ emission quantification in sectors, such as agriculture, energy, and waste management, where traditional monitoring techniques may be impractical or limited. Full article

Biogas is a renewable energy source produced by anaerobic digestion of organic waste. It can be upgraded to bio-methane by removing carbon dioxide, water and impurities. The present work focuses on carbon dioxide removal using both physical and chemical absorption in a micromixer. The absorption efficiency in the micromixer was studied under various conditions of co-current gas–liquid flow. With physical absorption, 25% of carbon dioxide could be removed from the biogas stream (with a liquid flowrate of 40 mL/min and a gas flowrate of 25 mL/min). In absorption with a chemical reaction, up to 88% of the carbon dioxide was eliminated with a catalyst concentration of 77.4 mol·m⁻³. In both cases, the space time was below 3 s. Liquid-side mass transfer coefficients as large as 3.5 s⁻¹ were achieved, which is at least two orders of magnitude higher than those reported in conventional absorbers. Full article

An in vitro and an in vivo study were conducted to investigate the effects of a blend of cinnamaldehyde, eugenol, and capsicum oleoresin (CEC) on rumen fermentation parameters, animal performance, and methane (CH₄) emissions in dairy cows. Continuous culture fermenters (CCF) were utilized to test one of two treatments: (1) CON; no supplementation and (2) CEC supplemented at 0.0125 g/d. The basal diet consisted of grass hay and concentrate (50:50). Supplementation with CEC increased (p < 0.01) total volatile fatty acids (VFA; mM) and decreased (p = 0.02) CH₄ concentration compared with CON in vitro. Additionally, protozoa abundance tended (p = 0.07) to decrease in CEC compared with CON. The in vivo experiment utilized forty Holstein-Friesian dairy cows (32% primiparous and 68% multiparous) averaging 163 ± 48 days in milk (DIM) and 38 ± 6.2 kg/d of milk yield (MY). Cows were blocked by parity and randomly assigned to one of two treatments: (1) CON; no supplementation and (2) CEC supplemented at 1.2 g/cow/d. The basal diet consisted of grass hay and concentrate (40:60). Individual CH₄ emissions were recorded using the sniffer technique. Dry matter intake (DMI) and eating rate were increased (p < 0.01; 3.6% and 5.2%, respectively), while feed efficiency decreased (p < 0.05) in CEC compared with CON. Additionally, CEC decreased (p = 0.02) CH₄ yield by 16.4% and tended to reduce daily CH₄ production (p = 0.09) and CH₄ intensity (p = 0.08) by 13.4% and 14.0%, respectively. Supplementing CEC decreased CH₄ concentration in vitro and CH₄ yield in vivo without negatively impacting performance parameters. Full article

This study assesses the energy, economic, and environmental implications of switching Tanzania’s road transport sector to natural gas, which is slowly transitioning. In energy, the main goal is to identify the energy demand for petroleum fuel (diesel and petrol) and natural gas during the transition, while in the economy, the government revenue in the form of taxes for shifted and unshifted vehicles, as well as the loss in government revenue from petroleum fuel revenue post-transition, is assessed. In the environment, carbon emission in terms of carbon dioxide equivalent (CO_2e), carbon tax revenues, and carbon credit revenues post-transition is estimated. The shift involved 10, 20, and 30% of the road vehicle population. The 10, 20, and 30% shift targeted about 142,247, 183,893, and 225,540 vehicles, which in turn dropped diesel and petrol demand by 7 and 3.68%, 7 and 3.8%, and 15 and 7.5%, respectively. In natural gas, the demand started at 0.0916 billion kg and grew exponentially by 200% and later by 300%. The transition has consequences in government revenue, which takes the form of taxes on petroleum products. The shift from 10 to 30% could lead to foregone taxes amounting to Tanzania shilling TZS 0.09, 0.31, and 0.54 trillion (US$ 33,358,680, US$ 11,490,212, and US$ 20,015,208), indicating a tax loss of about 3, 9, and 15%. Contrary, the government may benefit from these losses by lowering the amount of foreign currency necessary for oil importation. In environmental benefits, the 10, 20, and 30% shift could offset approximately 8,959,198.92119, 8,438,863.65528, and 7,918,528.38937 tCO_2e, equivalent to 5.4, 10.97, and 16.47% of the road emissions. The post-transition road emissions might result in a carbon tax revenue of about US$ 71,673,591.37, 67,510,909.24, and 63,348,227.11 per year. The post-transition carbon credit revenue of about US$ 20,813,410.64, 41,626,821.27, and 62,440,231.91 is expected annually. The findings are critical for policy design and promoting a transition in the road transport sector. Full article

The present study investigates a two-stage process aimed at producing biogas from food waste leachates (FWL) through an experimental approach. The first stage involves biohydrogen production via dark fermentation (DF), while the second focuses on biomethane production through anaerobic digestion (AD). The substrate consists of leachates derived from fruit and vegetable waste, which are introduced into two continuous stirred-tank reactors (CSTR1) with two different inoculum-to-substrate ratios (ISR). Dark fermentation occurs in these reactors. The effluent from the CSTRs is then fed into two additional reactors for methanogenesis. All reactors operated under mesophilic conditions. During the DF stage, hydrogen yields were relatively low, with a maximum of 8.2 NmL H₂/g VS added (ISR = 0.3) and 6.1 NmL H₂/g VS added (ISR = 0.5). These results were attributed to limited biodegradation of volatile solids (VS), which reached only 21.9% and 23.6% in each respective assay. Similarly, the removal of organic matter was modest. In contrast, the AD stage demonstrated more robust methane production, achieving yields of 275.2 NmL CH₄/g VS added (ISR = 0.3) and 277.5 NmL CH₄/g VS added (ISR = 0.5). The system exhibited significant organic matter degradation, with VS biodegradability reaching 66%, and COD removal efficiencies of 50.8% (ISR = 0.3) and 60.1% (ISR = 0.5). The primary focus of the study was to monitor and quantify the production of the two biofuels, biohydrogen and biomethane. In conclusion, this study provides an assessment of the two biochemical conversion pathways, detailing the generation of two valuable and utilizable gaseous products. This research examines the process-specific operational conditions governing gas production, with a focus on optimizing process parameters to enhance yield and overall efficiency. Full article

Ruminal methanogenesis represents considerable energy loss within the fermentative processes mediated by microbial populations, by means of which up to 12% of gross energy intake is driven away from microbial protein synthesis (MPS). This review explores the relationship between methane (CH₄) synthesis and emission with MPS in beef cattle, focusing on the nutritional, biochemical, and microbial factors modulating these processes. The synthesis of CH₄ by ruminal archaea is essential for maintaining redox balance during the fermentation of carbohydrates. This process diverts metabolic H₂ from energy-efficient pathways like propionate synthesis, which could otherwise enhance microbial growth. Dietary factors, including carbohydrate fermentability, N synchronization, and passage rate, modulate MPS. Diets based on roughage might enhance CH₄ synthesis while impairing MPS efficiency by reducing diet digestibility and promoting microbial shifts towards methanogenic populations. Potential mitigation strategies, including plant secondary metabolites, CH₄ inhibitors, and controlled forage-to-concentrate ratios, demonstrate the potential to reduce CH₄ emissions while enhancing nutrient utilization. This review underscores the need for integrated approaches combining dietary strategies, advanced feed additives, and improved prediction models to optimize ruminal fermentation, enhance MPS, and reduce the environmental footprint of beef cattle systems. Full article

Methane, a potent greenhouse gas, has a global warming potential over 84 times greater than carbon dioxide over its relevant lifespan. Current atmospheric methane concentrations are at a record high, significantly contributing to near-term climate warming. Agriculture, particularly livestock, is a major methane emitter, accounting for 40% of global total emissions, with enteric fermentation in ruminants accounting for 90% of agricultural methane emissions. The recent interest in mitigating these emissions has centered on seaweeds, such as Asparagopsis taxiformis, which contain bromoform, a bioactive compound shown to significantly reduce enteric methane production. However, bromoform raises environmental concerns including its potential carcinogenicity and ozone-depletion effects. This study systematically reviews the environmental and ozone-related impacts of scaling up seaweed production for enteric methane reduction in livestock. Key challenges include sustainability, biodiversity risks, and upstream emissions possibly offsetting the methane reduction gains. Animal health concerns, such as reduced weight gain and mucosal irritation, also warrant attention. Additionally, supply chain logistics, cultivation and harvesting practices, and bromoform retention remain underdeveloped. The limited assessment of the ozone depletion potential underscores the need for further research. These findings highlight the need for techno-feasibility and life cycle assessment before scaling up seaweed-based solutions. A broader approach to methane mitigation, beyond feed additives, is essential to ensure sustainable outcomes for livestock agriculture. Full article

Anaerobic digesters fed with dairy cow slurry struggle to achieve economic viability, particularly when animals are housed seasonally, so additional feedstocks are usually required. This study applied experimentally derived data from the co-digestion of cow slurry (CS) and food waste (FW) to the UK dairy herd as a whole, and at average (AH) and large (LH) herd sizes of 160 and 770 animals, respectively. The experimental data confirmed stable operation at an organic loading rate (OLR) of 5 g VS L⁻¹ day⁻¹ at CS:FW ratios of 3:1 and 6:1 on a wet weight basis, and these parameters were considered for both AH and LH by herd size and country (Scotland, England, Wales, Northern Ireland) in order to provide energy production and policy observations. The results showed that these scenarios could provide between 959 to 23,867 GJ per year, and that a targeted policy intervention could affect slurry treatment from a significant number of animals in a relatively small number of large herds across the UK. For a more detailed analysis, better data are required on non-domestic FW arisings and FW transportation needs. Full article

This study evaluates the energy recovery from biogas generated through the anaerobic co-digestion of the Organic Fraction of Urban Solid Waste (OFUSW) with lime mud (LM). This approach aims to mitigate environmental impacts such as greenhouse gas emissions and pollution while promoting energy recovery for a diversified power matrix. Life cycle assessment (LCA) methodology, in accordance with the NBR ISO 14040 and 14044 standards, was used to compare five scenarios for the disposal of LM. The results highlight that the co-digestion scenario showed significant environmental benefits in 8 out of the 18 categories evaluated, such as reductions in eutrophication, acidification, and climate change. Additionally, the digestate produced helped avoid further environmental impacts. The integration of urban and industrial waste demonstrates the potential to enhance biogas productivity, generate savings for the pulp and paper industry, and promote sustainable practices. The innovation lies in the synergistic use of LM as a co-substrate, improving the efficiency of the anaerobic process and maximizing biogas production. This research provides a solid scientific foundation for decision-making in public policies and industrial practices, positioning itself as a viable and innovative proposal for the integrated management of solid waste and sustainable energy. Full article

This study employs DFT at the APFD/def2-TZVP level, with SMD solvation in THF, to investigate the catalytic activation of methane by [(κ³-CNC)Fe(N₂O)]²⁺ cation complexes. The catalytic mechanism encompasses three key steps: oxygen atom transfer (OAT), hydrogen atom abstraction (HAA), and oxygen radical rebound (ORR). The computational results identify OAT as the rate-determining step, with activation barriers of −10.2 kcal/mol and 5.0 kcal/mol for κ¹-O- and κ¹-N-bound intermediates in the gas and solvent phases, respectively. Methane activation proceeds via HAA, with energy barriers of 16.0–25.2 kcal/mol depending on the spin state and solvation, followed by ORR, which occurs efficiently with barriers as low as 6.4 kcal/mol. The triplet (S = 1) and quintet (S = 2) spin states exhibit critical roles in the catalytic pathway, with intersystem crossing facilitating optimal reactivity. Spin density analysis highlights the oxyl radical character of the Fe^IV=O intermediate as being essential for activating methane’s strong C–H bond. These findings underscore the catalytic potential of CNC-ligated iron complexes for methane functionalization and demonstrate their dual environmental benefits by utilizing methane and reducing nitrous oxide, a potent greenhouse gas. Full article

The effects of milling on the anaerobic degradability of wheat straw and corn stover were investigated. Pretreatment was carried out by an industrial-scale device, able to process over one ton per hour. After 28 days of digestion under mesophilic conditions, the cumulative methane production from the pretreated straw (250 Nm³ t⁻¹ of volatile solids) was 49.2% greater than that from the raw material. Pretreated stover reached a cumulative methane yield of 219.8 Nm³ t⁻¹ of volatile solids, gaining 10.1% as compared to the feedstock. The specific electrical energy requirements for pretreatment were 66.6 kWh t⁻¹ for processed straw and 64.8 kWh t⁻¹ for stover; these consumptions were not significantly different. With reference to biomethane production, the impact of raw material on the production cost decreased from EUR 0.418 Nm⁻³ to EUR 0.328 Nm⁻³ for pretreated straw, whereas it increased by 5.8% for corn stover, whose pretreatment, therefore, was not economically feasible. Full article

In order to solve the wall damage problem of roadways with deep and high stress in methane explosion accidents, mathematical-physical analysis models for the dynamic response damage of roadway walls were established by LS-Dyna software in this paper, and the models were validated to be effective. The roadway wall displacement, stress, and deformation characteristics under the methane explosion impact load were numerical simulated and the response and damage evolution process of the roadway wall was studied. The results indicate that the model established in this study can reflect the dynamic response damage characteristics of the roadway wall. The damage of the roadway wall caused by the methane explosion impact load was mainly concentrated in the methane accumulation section, but the maximum principal stress of the roadway wall near the methane accumulation section was still high, and the damage possibility was also high. The dynamic response damage of the roadway wall decreased with the increase in the distance from the initiation explosion point. The stress response of the curved part of the roadway roof was the most severe, and the stress response of the side part was second to that of the roof. The stress changes at the corners were significant, but the deformation was small. The bottom plate was minimally affected by the methane explosion impact loads. The arch top and two sides of the roadway were first subjected to significant impact, resulting in a high-pressure zone. The peak pressure of the side part was relatively high, and the difference in peak pressure between the corner and the bottom plate was not significant. Full article

Methane is a strong green gas that has higher GWP. Methane emissions, therefore, form one of the critical focuses within climate change mitigation policy. Indeed, the present study represents a very novel analysis of methane emission within the ESG framework by using the data across 193 countries within the period of 2011–2020. Methane reduction on account of ESG delivers prompt climate benefits and thereby preserves the core environment, social, and governance objectives. In spite of its importance, the role of methane remains thinly explored within ESG metrics. This study analyzes how factors like renewable energy use, effective governance, and socioeconomic settings influence the emission rate of the study subject, as many previous ESG studies are deficient in considering methane. By using econometric modeling, this research identifies that increasing methane emissions remain unabated with the improvement of ESG performances around the world, particularly within key agricultural and fossil fuel-based industrial sectors. Renewable energy cuts emissions, but energy importation simply transfers the burdens to exporting nations. It therefore involves effective governance and targeted internationational cooperation, as socioeconomic elements act differently in different developed and developing countries to drive various emission sources. These findings strongly call for balanced, targeted strategies to integrate actions of mitigation into ESG goals related to methane abatement. Full article

Steam reforming is an important industrial process for hydrogen production. Acetone, the by-product of phenol production from cumene peroxidation, is a useful source of hydrogen due to its availability and low value compared to hydrogen fuel. This study aimed to utilize the Gibbs free energy minimization method using the Soave–Redlich–Kwong (SRK) equation of state (EOS) to conduct a thermodynamic analysis of the steam reforming process for pure component acetone. The steam reforming process is temperature dependent, with increasing temperatures leading to higher hydrogen production. Competing reactions, particularly the exothermic reverse water–gas shift, impact hydrogen yields beyond 650 °C. The study identified 600 °C as the optimum temperature to strike a balance between maximizing hydrogen production and minimizing the reverse water–gas shift’s impact. The optimal hydrogen yield (70 mol%) was achieved at a steam-to-oil ratio (STOR) of 12. High STOR values shift the equilibrium of the water–gas shift reaction towards hydrogen production due to increased steam, effectively consuming acetone and favoring the desired product. Atmospheric pressure is optimum for hydrogen production because the equilibrium of gas phase reactions shifts in favor of the lighter components at lower pressures. Full article

The evaluation of measurement uncertainty of natural gas properties calculated from composition data are an essential aspect of fiscal metering in the trade of natural gas. For conformity assessment, and in gas allocation, it is essential to have a reliable value for the uncertainty. This need is also reflected in, e.g., ISO 6976, the standard for computing natural gas properties, which follows the requirements of the “Guide to the expression of uncertainty in measurement” much more closely. Normalised compositions and their associated standard uncertainties do not suffice for this purpose. A novel algorithm is provided to recover these correlations from the normalised fractions and associated standard uncertainties, enabling the industry work with the data already stored in their repositories. The standard uncertainties are reproduced within 2%, which is acceptable for uncertainty calculations. The correlation coefficients obtained from the recovery algorithm agree with the ones obtained by normalisation. Full article

This study aimed to obtain the energy recovery potential of the biogas produced from anaerobic digestion (AD) of the sludge from a wastewater treatment plant (WWTP), including the use of biochar as an additive for substrate co-digestion and catalyst for methane production. We carried out the following steps: chemical–physical laboratory analyses of sludge samples; the building, operation, and monitoring of an experimental prototype of a batch bioreactor of 2.5 L for the AD of the sludge (with and without the addition of charcoal); qualitative measurements of biogas; the study of charcoal morphology; and the projection of useful energy generation from the AD sludge after treatment. A study on the economic viability and avoided greenhouse gas (GHG) emissions was performed based on the experimental results. The substrate showed alterations in all the physicochemical parameters evaluated after AD, such as a reduction of 35% in the biochemical oxygen demand (BOD) analysis; the experiment carried out using biochar showed positive results regarding the speed of CH₄ production and a greater potential for energy recovery. Enterprises from 2000 kW onwards would present an internal rate of return (IRR) equal to or higher than the minimum attractiveness rate (MAR) of 15%. The USD 95.28/MWh tariff presented economic feasibility for the studied scenarios. WWTPs that produce enough sludge to generate power of 2000 kW would need to process the waste of 117,200 inhabitants with charcoal addition and 136,000 without charcoal. It would be possible to avoid the emission of 2307.97 tCO₂/year (2000 kW). According to the results obtained, this study revealed that using alternative energies based on anaerobic digestion and biochar can generate positive results regarding methane production, and its application as an energy source in a WWTP proved to be economically viable at a specific level of power production. Full article

During the process of mining underground coal, the coal emits a large amount of methane into the mining space, which may lead to methane accumulation and exceed explosion safety limits When the methane encounters a fire source, a methane explosion may occur. The forceful impact caused by a methane explosion in an underground roadway can cause serious damage to the roadway structures and even lead to the collapse of the ventilation system. At the same time, the explosion impact may result in the death of workers and cause physical injury to the surviving workers. Therefore, it is necessary to study the damage effect and injury range of methane explosions. On the basis of the damage criteria and damage characteristics of methane explosions, according to the overpressure distribution of shock waves in the propagation process of a methane explosion, the explosion hazard range is divided into four ranges (from inside to outside): death range, serious injury range, minor injury range, and safety range. Four injury degrees of shock wave overpressure to personal body (slight, medium, serious injury, death), and seven damage degrees of overpressure to structures are also analyzed. The thresholds of their damage (destruction) are determined. On this basis, an experimental system and numerical simulation are constructed to measure damage characteristics, the overpressure value, and the range distance of a methane explosion with different initial explosion intensities. According to the experimental and numerical results, the attenuation formula of a methane explosion shock wave in the propagation process is derived. The research results show that the overpressure and impulse of shock waves are selected as the damage criteria for comprehensive evaluation, and the overpressure criterion is suitable of determining the injury (failure) range over long distances. The four injury ranges are in line with the actual situation and are reasonable. The injury degree also conforms to the medical results, which can be used to guide the injury degree of mine methane explosions. The injury range caused by methane explosions with different initial explosion intensities is reasonable and is basically consistent with the on-site situation. The derived attenuation formula and calculated safety distance are in good agreement with the experimental and numerical results. The research results can provide guidance and help in the escape, rescue, and protection of coal mine underground person. Full article