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Neutral red (NR) is a phenazine dye that has been implicated in electron transfer processes in methanogenic archaea. NR has been previously observed to enhance methane production but its effects on Methanosarcina barkeri are unknown. This study aimed to investigate the effects of NR on M. barkeri DSM-804. M. barkeri cultures were grown in the presence of 10 and 250 µM NR for four weeks, and proteomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results showed that methane production was significantly reduced in the presence of NR, at lower concentrations of both 10 and 250 µM NR treatments, compared to the control. Proteomic analysis revealed the downregulation of proteins related to substrate metabolism and methanogenesis, such as the heterodisulfide reductase subunits D (HDRD_METBF) and E (HDRE_METBF), suggesting that NR hindered essential metabolic processes. Proteomic analysis also revealed that M. barkeri lacked methanophenazine in its membrane, which is a component essential for electron transport via neutral red (NR) that supports enhanced growth and methane production. Further research is needed to explore the role of methanophenazine and understand the mechanisms underlying NR’s effects of NR on methanogenesis in M. barkeri.

19 December 2025

Adapted from MetaCyc Pathway: acetoclastic methanogenesis. The numbers such as 2.7.2.1, 2.7.2.15, 2.3.1.8, 2.3.3.-, 2.1.1.-, 2.3.3.- are EC numbers assigned by the International Union of Biochemistry and Molecular Biology (IUBMB). EC 2.7.2.1/2.7.2.15—Acetate kinase; EC 2.3.1.8—Phosphotransacetylase; EC 2.3.3.-—Acetyl-CoA decarbonylase/synthase; EC2.1.1.-—Methyl-H4SPT:CoM methyltransferase (Mtr); EC 1.2.7.- (shown as 2.3.3.- in MetaCyc shorthand—Carbon monoxide dehydrogenase.

Agriculture is a significant contributor to greenhouse gas (GHG) emissions, with enteric methane (EntCH4) from cattle production being a major source. In Zambia, cattle play a critical role in rural livelihoods and food security, yet the contribution of cattle production systems to national GHG emissions remains poorly quantified. This study used the Intergovernmental Panel on Climate Change (IPCC) Tier 2 method to estimate EntCH4 from Zambia’s cattle population from 1994 to 2022. The Tier 2 method provides a more accurate estimate than the Tier 1 method by incorporating country-specific data on cattle population demographics, husbandry, and feeding practices. The results show significant variations in EntCH4 over time, driven by changes in cattle population dynamics and production practices. This study underscored the importance of transitioning from the generalized Tier 1 to the Tier 2 method to capture the unique characteristics of Zambia’s cattle production systems. The present findings provide critical insights for developing targeted mitigation strategies that will support Zambia’s ongoing efforts to address climate change while promoting sustainable livestock production.

15 December 2025

Using Methane to Support Renewables for Decarbonisation

  • Stephen A. Lloyd and
  • William J. Atteridge

The cost of “carbon net zero by year 2050” for the UK will be high, and this target date can only be achieved if the project is undertaken in a progressive and timely manner; otherwise, costs will escalate. The base power source behind the UK approach to “net zero” is nuclear fission electricity power stations, and the ones currently on order are running significantly late. Renewables will provide some supply together with interconnectors, but only approx. twenty percent of the planned wind turbines are in place. The electricity distribution grid must change to satisfy the UK’s planned “electricity-based” future. Energy use for transport is also a significant fraction of total UK energy consumption and we include predictions for their associated emissions. These must be reduced in a progressive and timely fashion. Intermittent support for unreliable renewables is necessary and methods employing both liquid as well as gaseous fuels are suggested. Means to use and upgrade the existing infrastructure are considered, and a few of the basic building blocks of the future are examined regarding their installation without significant interruption to the basic UK economy. ANR/AMR and SMR are included as potential renewables support as well as base load generators, and the approx. quantity of CO2e emissions avoided is estimated. Even though methane is a powerful greenhouse gas, the main support for renewables will be UK natural gas (methane content ~95%), with Avtur/diesel as a recommended reserve. It is suggested that methane has a significant short- to medium-term future as a transition fuel.

12 December 2025

Rice cultivation accounts for roughly 10% of worldwide anthropogenic greenhouse gas emissions, making it a significant source of methane (CH4) Despite modest observational constraints, estimates of worldwide CH4 emissions from rice agriculture range from 18–115 Tg CH4 yr−1. CH4 is a potent greenhouse gas, and its oxidation produces tropospheric ozone (O3), which is harmful to public health and crop production when combined with nitrogen oxides (NOx) and sunlight. Elevated O3 levels reduce air quality, crop productivity, and human respiratory health. This study presents an AI-driven framework that combines ensemble learning, hyperparameter optimisation (HPs), and SHAP-based explainability to enhance CH4 emission predictions from rice paddies in India, Bangladesh, and Vietnam. The model consists of two stages: (1) a classification stage to distinguish between zero and non-zero CH4 emissions, and (2) a regression stage to estimate emission magnitudes for non-zero situations. The framework also incorporates O3 and asthma incidence data to assess the downstream impacts of CH4-driven ozone formation on air quality and health outcomes. Understanding the factors that drive optimal model performance and the relative importance of features affecting model outputs is still an ongoing field of research. To address these issues, we present an integrated approach that utilises recent improvements in model optimisation and employs SHapley Additive ExPlanations (SHAP) to find the most relevant variables affecting methane (CH4) emission forecasts. In addition, we developed a web-based artificial intelligence platform to help policymakers and stakeholders with climate strategy and sustainable agriculture by visualising methane fluxes from 2018 to 2020, ensuring practical applicability. Our findings show that ensemble learning considerably improves the accuracy of CH4 emission prediction, minimises uncertainty, and shows the wider benefits of methane reduction for climate stability, air quality, and public health.

12 November 2025

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Methane - ISSN 2674-0389