Diagnosing and treating many infectious diseases depends on correctly identifying the causative pathogen. Characterization of pathogen-specific nucleic acid sequences by PCR is the most sensitive and specific method available for this purpose, althou...
Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. Its success is based on fluorescence of organic dye molecules under carefully engineered laser illumination. In this paper we d...
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological...
The stems of Dendrobium officinale Kimura et Migo, named Tie-pi-shi-hu, is one of the most endangered and precious species in China. Because of its various pharmacodynamic effects, D. officinale is widely recognized as a high-quality health food in...
Supplemental lighting with light-emitting diode (LED) lamps and/or high-pressure sodium (HPS) lamps was applied to increase the activity of the photosynthetic apparatus and thus productivity of greenhouse cucumber (Cucumis sativus L.) in a high-wire...
The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon...
Glutathione (GSH) is an antioxidant thiol that has a vital role in the pathogenesis of various human diseases such as cardiovascular disease and cancer. Hence, it is necessary to study effective methods of GSH evaluation. In our work, an effective GS...
Potassium (K+) is the most abundant cation in the cytosol and is maintained at high concentrations within the mitochondrial matrix through potassium channels. However, many effects of K+ at such high concentrations on mitochondria and the underlying...
Excessive estrogen exposure is connected with increased risk of breast cancer and has been shown to promote epithelial-mesenchymal-transition. Malignant cancer cells accumulate changes in cell mechanical and biochemical properties, often leading to c...
Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed...
Oxidized regenerated cellulose (ORC) is an approved absorbable hemostat in neurosurgery, and contains 18–21% carboxylic acid groups. This modification leads to a low pH in aqueous solutions. Therefore, the aim of study was to analyze the pH-dep...
The study of growth and possible metastasis in animal models of tumors would benefit from reliable cell labels for noninvasive whole-organism imaging techniques such as magnetic resonance imaging. Genetically encoded cell-tracking reporters have the...
The global market shows high demand for products of natural origin to reduce the use of synthetic substances in the food, pharmaceutical and cosmetics industries. One of the opportunities for acquiring natural compounds of industrial value is the use...
Research progresses have led to the development of different kinds of nanoplatforms to deliver drugs through different biological membranes. Particularly, nanocarriers represent a precious means to treat skin pathologies, due to their capability to s...
High-quality in-depth imaging of three-dimensional samples remains a major challenge in modern microscopy. Selective plane illumination microscopy (SPIM) is a widely used technique that enables imaging of living tissues with subcellular resolution. H...
The leaching effect of metals has led to the introduction of government regulations for the safety of the environment and humans. This has led to the search for new alloys with long-lasting sustainability. Herein, we wish to report a new brass alloy...
It has been shown that the cultivation of plants under glass coated with nano-sized upconversion luminophores led to an increase in plant productivity and the acceleration of plant adaptation to ultraviolet radiation. In the present work, we examined...
The development of MRI probes is of interest for labeling antibiotic-resistant fungal infections based on yeast. Our work showed that yeast cells can be labeled with high-spin Fe(III) complexes to produce enhanced T2 water proton relaxation. These Fe...
A novel and efficient method for synthesizing Au-decorated ZnO nanoparticles (NPs) with enhanced photocatalytic activity is presented. The synthesis involves a two-step process: hydrothermal preparation of ZnO NPs followed by nonthermal plasma-assist...
Advances in antimicrobial activities of molecule-containing, multiple guanidinium groups against antibiotics-resistant bacteria should be noted. The synthesized polyoctamethylene monoguanidine hydrochloride (POGH), carrying cationic amphiphilic moiet...
Non-invasive detection of polarized macrophages in tumors is an urgent task in terms of combined antitumor therapy. By analyzing the fluorescence lifetime of the metabolic cofactors—the reduced form of nicotinamide adenine dinucleotide (NADH) a...
New fluorescent systems for photocatalysis, sensors, labeling, etc., are in great demand. Amphiphilic ones are of special interest since they can form functional colloidal systems that can be used in aqueous solutions. A new macrocycle platform for c...
Cellular memory is a critical ability that allows microorganisms to adapt to potentially detrimental environmental fluctuations. In the unicellular eukaryote Saccharomyces cerevisiae, cellular memory can take the form of faster or slower responses wi...
Multidrug-resistant Cryptococcus neoformans is an encapsulated yeast causing a high mortality rate in immunocompromised patients. Recently, the synthetic peptide Mo-CBP3-PepII emerged as a potent anticryptococcal molecule with an MIC50 at low concent...
In contrast to enamel, dentin surfaces have been rarely used as substrates for studies evaluating the effects of experimental rinsing solutions on oral biofilm formation. The aim of the present in situ study was to investigate the effects of tannic a...
Biological applications of silver nanoparticles (AgNPs) depend on the covalently attached or adsorbed proteins. A series of biological effects of AgNPs within cells are determined by the size, shape, aspect ratio, surface charge, and modifiers. Herei...
This study investigated the antiviral activity of aqueous leaf extract of Costus speciosus (TB100) against influenza A. Pretreatment of TB100 in RAW264.7 cells enhanced antiviral activity in an assay using the green fluorescence-expressing influenza...
This chapter describes in summary form some of the most important research on chromosome segregation, from the discovery and naming of mitosis in the nineteenth century until around 1990. It gives both historical and scientific background for the nin...
The aim of this study was to prevent initial changes in Drosophila melanogaster oocytes under simulated weightlessness and hypergravity at the 2 g level. Phospholipids with polyunsaturated fatty acids in the tail groups (essential phospholipids) at a...
Recent developments in microfluidic devices, nanoparticle chemistry, fluorescent microscopy, and biochemical techniques such as genetic identification and antibody capture have provided easier and more sensitive platforms for detecting and diagnosing...
The effects of ultrasound on the molecular weight distribution and emulsifying properties of both xanthan gum (XG) and propylene glycol alginate (PGA) were investigated. The results showed that ultrasonic treatment at different intensities decreased...
The electrorheological (ER) properties of composite materials consisting of a nematic liquid crystal (LC) and gold nanoparticles (GNPs) coated with bistolane-based mesogenic groups were studied. The GNPs were coated by normal alkyl chains and the flu...
In recent years several approaches have been developed to address the chromatin status and its changes in eukaryotic cells under different conditions—but only few are applicable in living cells. Fluorescence lifetime imaging microscopy (FLIM) i...
Fungal secretomes are known to contain a multitude of components involved in nutrition, cell growth or biotic interactions. Recently, extra-cellular vesicles have been identified in a few fungal species. Here, we used a multidisciplinary approach to...
Mortars from the cistern in Amaiur Castle (Navarre, Spain) were analysed to assess the mortar manufacturing process and application techniques. To this end, optical microscopy, scanning electron microscopy (SEM), thermogravimetric analysis, X-ray flu...
Fluorescence microscopy was applied to understand adhesion interfaces developed within laminated composite sandwiches formed between poly(lactic acid) (PLA) and wood veneers. Composites formed with maple veneer had greater tensile bond strength when...
This study explores the potential anticancer effects of lesbicoumestan from Lespedeza bicolor against human leukemia cancer cells. Flow cytometry and fluorescence microscopy were used to investigate antiproliferative effects. The degradation of mucos...
Background: Recent studies evidence that blue-LED-light irradiation can modulate cell responses in the wound healing process within 24 h from treatment. This study aims to investigate blue-light (410–430 nm) photobiomodulation used in a murine...
The subject of Mg-based biodegradable materials, used for medical applications, has been extensively studied throughout the years. It is a known fact that alloying Mg with biocompatible and non-toxic elements improves the biodegradability of the allo...
The ongoing tendency to create environmentally friendly building materials is nowadays connected with the use of reactive magnesia-based composites. The aim of the presented research was to develop an ecologically sustainable composite material based...
Two macrocyclic Schiff bases derived from o-phenylenediamine and 2-hydroxy-5-methylisophthalaldehyde L1 or 2-hydroxy-5-tert-butyl-1,3-benzenedicarboxaldehyde L2, respectively, were obtained and characterized by X-ray crystallography and spectroscopy...
Hemorrhage, particularly non-compressible torso bleeding, remains the leading cause of preventable death in trauma. Self-propelling hemostats composed of thrombin-calcium carbonate (CaCO3) particles and protonated tranexamic acid (TXA+) have been sho...
In this study, we investigated the inhibitory effects of emodin on pyroptosis in rheumatoid arthritis (RA) synovial cells by modulating the HIF-1α/NLRP3 inflammasome pathway and mitochondrial autophagy. By employing a chemically induced hypoxia...
Mycenaean glass artifacts, such as beads and relief plaques, are highly susceptible to degradation, which can significantly modify their visual attributes and pose classification challenges. Corrosion on glass and faience artifacts has often led to m...
Recently, fluorescent sensors have gained considerable attention due to their high sensitivity, low cost and noninvasiveness. Among the different materials that can be used for this purpose, carbon dots (CDs) represent valuable candidates for applica...
Bacterial resistance to β-lactam antibiotics, particularly new generation cephalosporins, is a major public health concern. In Escherichia coli, resistance to these antibiotics is mainly mediated by extended-spectrum β-lactamases (ESBL), wh...
Adipose tissue plays a crucial role in the tumor microenvironment (TME), where its secreted extracellular vesicles (EVs) are involved in the complex signaling between tumor cells and surrounding stromal components. This study aims to unravel the mech...
Atmospheric-pressure low-temperature plasma (APLTP) was used to study the bactericidal mechanism against Escherichia coli (E. coli) and its application in the sterilization of fresh-cut cucumbers. The morphological changes of E. coli cells subjected...
Advances in medicinal chemistry have led to the development of anticancer and anti-infectious drugs. However, many types of cancer and viral infections such as hepatitis B virus or SARS-CoV-2 are still treated ineffectively. Therefore, further develo...
Virus filtration is an essential unit operation used to validate clearance of adventitious virus during the manufacture of biopharmaceutical products such as monoclonal antibodies. Obtaining at least a 10,000-fold reduction in virus particles in the...
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