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Open AccessArticle

Hyperosmotic Stress Response Memory is Modulated by Gene Positioning in Yeast

Université de Paris, Laboratoire Matière et Systèmes Complexes, CNRS UMR 7057, F-75013 Paris, France
Université de Paris, Laboratoire Génomes, Biologie Cellulaire et Thérapeutiques, CNRS UMR7212, INSERM U944, Centre de Recherche St Louis, F- 75010 Paris, France
Authors to whom correspondence should be addressed.
Cells 2019, 8(6), 582;
Received: 15 May 2019 / Revised: 11 June 2019 / Accepted: 12 June 2019 / Published: 13 June 2019
(This article belongs to the Special Issue Nucleolar Organization and Functions in Health and Disease)
Cellular memory is a critical ability that allows microorganisms to adapt to potentially detrimental environmental fluctuations. In the unicellular eukaryote Saccharomyces cerevisiae, cellular memory can take the form of faster or slower responses within the cell population to repeated stresses. Using microfluidics and fluorescence time-lapse microscopy, we studied how yeast responds to short, pulsed hyperosmotic stresses at the single-cell level by analyzing the dynamic behavior of the stress-responsive STL1 promoter (pSTL1) fused to a fluorescent reporter. We established that pSTL1 exhibits variable successive activation patterns following two repeated short stresses. Despite this variability, most cells exhibited a memory of the first stress as decreased pSTL1 activity in response to the second stress. Notably, we showed that genomic location is important for the memory effect, since displacement of the promoter to a pericentromeric chromatin domain decreased the transcriptional strength of pSTL1 and led to a loss of memory. This study provides a quantitative description of a cellular memory that includes single-cell variability and highlights the contribution of chromatin structure to stress memory. View Full-Text
Keywords: chromosome organization; cellular memory; single cell; stress response; yeast chromosome organization; cellular memory; single cell; stress response; yeast
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Ben Meriem, Z.; Khalil, Y.; Hersen, P.; Fabre, E. Hyperosmotic Stress Response Memory is Modulated by Gene Positioning in Yeast. Cells 2019, 8, 582.

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