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  • Article
  • Open Access
4 Citations
3,515 Views
19 Pages

CLSM-Guided Imaging for Quantifying Endodontic Disinfection

  • Rebecca Mattern,
  • Sabrina Ernst,
  • Sarah Böcher,
  • Andreas Braun,
  • Johannes-Simon Wenzler and
  • Georg Conrads

Elimination of microbes in the root canal system is crucial for achieving long-term success in endodontic treatment. Further efforts in study design and standardization are needed in order to improve the validity and comparability of in vitro results...

  • Article
  • Open Access
545 Views
17 Pages

CLSM-Guided Imaging to Visualize the Depth of Effective Disinfection in Endodontics

  • Rebecca Mattern,
  • Sarah Böcher,
  • Gerhard Müller-Newen,
  • Georg Conrads,
  • Johannes-Simon Wenzler and
  • Andreas Braun

1 December 2025

Background/Objectives: Important goals of endodontic treatment procedures are to effectively eliminate microorganisms from the root canal system and prevent reinfection. Despite advances in techniques, these goals continue to be difficult to achieve...

  • Article
  • Open Access
4 Citations
1,295 Views
14 Pages

The Influence of CLSM Magnification on the Measured Roughness of Differently Prepared Dental Materials

  • Martin Rosentritt,
  • Anne Schmutzler,
  • Sebastian Hahnel and
  • Laura Kurzendorfer-Brose

5 December 2024

This in vitro study investigated how varying magnifications (5×, 10×, 20×, and 50×) using a confocal laser scanning microscope (CLSM) influence the measured surface roughness parameters, Ra/Sa and Rz/Sz, of various materials w...

  • Article
  • Open Access
1 Citations
4,836 Views
11 Pages

Mosaic-CLSM Assessment of Bacterial Spatial Distribution in Cosmetic Matrices According to Matrix Viscosity and Bacterial Hydrophobicity

  • Samia Almoughrabie,
  • Chrisse Ngari,
  • Romain Briandet,
  • Valérie Poulet and
  • Florence Dubois-Brissonnet

The reliability of the challenge test depends, among other parameters, on the spatial distribution of microorganisms in the matrix. The present study aims to quickly identify factors that are susceptible to impair a uniform distribution of inoculated...

  • Article
  • Open Access
1 Citations
928 Views
24 Pages

The large-scale reuse of shield tunneling muck remains a major challenge in urban construction. This study proposes a geopolymeric-controlled low-strength material (GC-CLSM) utilizing shield tunneling muck as the primary raw material and a novel alka...

  • Article
  • Open Access
18 Citations
7,636 Views
22 Pages

Application Prospects of FTIR Spectroscopy and CLSM to Monitor the Drugs Interaction with Bacteria Cells Localized in Macrophages for Diagnosis and Treatment Control of Respiratory Diseases

  • Igor D. Zlotnikov,
  • Alexander A. Ezhov,
  • Maksim A. Vigovskiy,
  • Olga A. Grigorieva,
  • Uliana D. Dyachkova,
  • Natalia G. Belogurova and
  • Elena V. Kudryashova

12 February 2023

Visualization of the interaction of drugs with biological cells creates new approaches to improving the bioavailability, selectivity, and effectiveness of drugs. The use of CLSM and FTIR spectroscopy to study the interactions of antibacterial drugs w...

  • Article
  • Open Access
8 Citations
2,490 Views
13 Pages

1 December 2022

Styrene–butadiene–styrene (SBS) polymer is extensively employed for asphalt pavement construction, and its degradation significantly damages the durability of asphalt concrete. However, the effect of aging protocols on the degradation of...

  • Article
  • Open Access
13 Citations
3,662 Views
18 Pages

4 March 2019

This study investigated the mix design and engineering properties of controlled low-strength material (CLSM) by partial replacement of fine aggregate using water purification sludge (WPS). First, an investigation was performed at laboratory scale to...

  • Article
  • Open Access
1 Citations
1,476 Views
23 Pages

1 July 2025

Conventional controlled low-strength material (CLSM) is a self-consolidating cementitious material with high flowability and low strength, traditionally composed of cement, sand, and water. This study explores the sustainable utilization of off-speci...

  • Article
  • Open Access
21 Citations
3,154 Views
12 Pages

2 June 2021

Fly ash, the main component for controlled low-strength material (CLSM), has physical and chemical characteristics according to the resources used in the thermal power plant, and thus fly ash type can influence the physical and strength properties of...

  • Article
  • Open Access
21 Citations
3,408 Views
21 Pages

23 September 2020

In this study, the effects of the mixing conditions of waste paper sludge ash (WPSA) on the strength and bearing capacity of controlled low-strength material (CLSM) were evaluated, and the optimal mixing conditions were used to evaluate the strength...

  • Article
  • Open Access
10 Citations
2,638 Views
16 Pages

6 February 2024

When backfilling narrow spaces, controlled low-strength materials (CLSM) can be used to achieve an effective backfilling effect. The pipeline engineering in Yahnghe Avenue of Suqian, China, provides a favorable on-site condition for the use of CLSM....

  • Article
  • Open Access
5 Citations
2,655 Views
10 Pages

A Feasibility Study for Immediate Histological Assessment of Various Skin Biopsies Using Ex Vivo Confocal Laser Scanning Microscopy

  • Hanna Ogrzewalla,
  • Matthias Möhrle,
  • Gisela Metzler,
  • Thomas Eigentler,
  • Anne-Kristin Münch and
  • Stephan Forchhammer

2 December 2022

Background: Digitally stained ex vivo confocal laser scanning microscopy (CLSM) scans are a possible alternative to formalin-fixed and paraffin-embedded (FFPE) and hematoxylin-eosin (H&E) stained slides. This study explores the diagnostic accurac...

  • Article
  • Open Access
8 Citations
1,715 Views
17 Pages

27 July 2023

Numerous studies have been conducted on fiber-reinforced concrete; however, comparative investigations specifically focusing on the utilization of fibers in CLSM remain limited. In this study, we conducted a systematic investigation into the mechanic...

  • Article
  • Open Access
22 Citations
3,620 Views
15 Pages

The Effect of Bottom Ash Ball-Milling Time on Properties of Controlled Low-Strength Material Using Multi-Component Coal-Based Solid Wastes

  • Tianxiang Chen,
  • Ning Yuan,
  • Shanhu Wang,
  • Xinfei Hao,
  • Xinling Zhang,
  • Dongmin Wang and
  • Xuan Yang

11 August 2022

As the conventional disposal method for industrial by-products and wastes, landfills can cause environmental pollution and huge economic costs. However, some secondary materials can be effectively used to develop novel underground filling materials....

  • Article
  • Open Access
30 Citations
4,707 Views
11 Pages

15 August 2018

The bottom ash of a municipal solid waste incinerator (MSWI) and water filter silt (WFS) were applied to a controlled low-strength material (CLSM) in the present study. The CLSM of the control group was composed of cement, water, and fine aggregates....

  • Article
  • Open Access
8 Citations
4,090 Views
15 Pages

Spinning Disk Confocal Microscopy for Optimized and Quantified Live Imaging of 3D Mitochondrial Network

  • Somaieh Ahmadian,
  • Patrick J. Lindsey,
  • Hubert J. M. Smeets,
  • Florence H. J. van Tienen and
  • Marc A. M. J. van Zandvoort

Mitochondria are the energy factories of a cell, and depending on the metabolic requirements, the mitochondrial morphology, quantity, and membrane potential in a cell change. These changes are frequently assessed using commercially available probes....

  • Article
  • Open Access
4 Citations
1,892 Views
15 Pages

11 February 2023

In this study, the optimum mixing ratio for backfill was determined by analyzing the mechanical properties and microstructure of controlled low-strength material (CLSM)―the backfill material for road excavation and restoration―using the t...

  • Interesting Images
  • Open Access
7 Citations
2,326 Views
8 Pages

In Vivo Monitoring of Corneal Dendritic Cells in the Subbasal Nerve Plexus during Trastuzumab and Paclitaxel Breast Cancer Therapy—A One-Year Follow-Up

  • Sebastian Bohn,
  • Nadine Stache,
  • Karsten Sperlich,
  • Stephan Allgeier,
  • Bernd Köhler,
  • Andreas Bartschat,
  • Ha-Vy Do,
  • Christian George,
  • Rudolf F. Guthoff and
  • Katharina Anna Sterenczak
  • + 2 authors

Paclitaxel and trastuzumab have been associated with adverse effects including chemotherapy-induced peripheral neuropathy (CIPN) or ocular complications. In vivo confocal laser scanning microscopy (CLSM) of the cornea could be suitable for assessing...

  • Proceeding Paper
  • Open Access
446 Views
9 Pages

Optical and Mechanical Characterization of Lignocaine-Impregnated Maltose-Based Dissolvable Microneedles

  • Arifah Syahirah Rahman,
  • Fook-Choe Cheah,
  • Mohd Eusoff Azizol Nashriby,
  • Mae-Lynn Catherine Bastion,
  • Chang Fu Dee,
  • Muhamad Ramdzan Buyong,
  • Mohd Ambri Mohamed,
  • Xin Yun Chua,
  • Poh Choon Ooi and
  • Azrul Azlan Hamzah
  • + 5 authors

14 January 2026

Dissolvable microneedles (DMNs) represent an innovative approach to patient-friendly drug delivery, eliminating the need for conventional hypodermic injections. This study reports on the fabrication, Confocal Laser Scanning Microscopy (CLSM)-based op...

  • Article
  • Open Access
1,671 Views
19 Pages

From Microstructure to Shade Shift: Confocal and Spectrophotometric Evaluation of Peroxide-Induced Dental Bleaching

  • Berivan Laura Rebeca Buzatu,
  • Magda Mihaela Luca,
  • Atena Galuscan,
  • Adrian Ovidiu Vaduva,
  • Aurora Doris Fratila,
  • Ramona Dumitrescu,
  • Ruxandra Sava-Rosianu,
  • Octavia Balean,
  • Roxana Buzatu and
  • Daniela Jumanca

1 July 2025

Background/Objectives: Tooth bleaching is a widely requested aesthetic procedure in modern dentistry. However, the structural effects of high-concentration peroxide-based bleaching agents on enamel remain insufficiently understood. This study aims to...

  • Article
  • Open Access
4 Citations
2,957 Views
11 Pages

Three-Dimension Resolution Enhanced Microscopy Based on Parallel Detection

  • Zhimin Zhang,
  • Shaocong Liu,
  • Minfei He,
  • Yuran Huang,
  • Cuifang Kuang,
  • Yubing Han,
  • Xiang Hao and
  • Xu Liu

22 March 2021

Pixel reassignment image scanning microscopy (PRISM) is a useful tool to improve the resolution of confocal laser scanning microscopy (CLSM) only equipped with a detector array. However, while it can improve the lateral resolution, it has little effe...

  • Article
  • Open Access
893 Views
15 Pages

Austenite Grain Growth Behavior and Dynamic Model of ADB790E Hydropower Steel

  • Yuxuan Liu,
  • Hongmei Zhang,
  • Zhongxiang Yu and
  • Zhengyi Jiang

17 September 2025

The growth behavior of austenite grains in ADB790E hydropower steel under the synergistic effects of heating temperature and holding time was studied using in situ quenching and an in situ high-temperature confocal laser scanning microscope (HT-CLSM)...

  • Article
  • Open Access
34 Citations
4,592 Views
11 Pages

2 May 2018

This study aims to investigate the effect of adding circulating fluidized bed combustion (CFBC) ash, desulfurization slag, air-cooled blast-furnace slag and coal bottom ash to the controlled low-strength material (CLSM). Test methods include slump fl...

  • Communication
  • Open Access
9 Citations
5,443 Views
8 Pages

2 December 2021

Glass fiber is widely used in various modern industrial applications because it has the advantages of good electrical insulation performance and good process ability. Inevitably, some flawed glass fiber generated during manufacturing processes become...

  • Article
  • Open Access
2 Citations
1,430 Views
8 Pages

21 July 2023

Magnesia is one of the vital and extensively used refractory components. In this study, the dissolution of magnesia is investigated at 1450, 1500, and 1550 °C in three silicate slags in the CaO–Al2O3–SiO2–MgO system using high-t...

  • Article
  • Open Access
25 Citations
3,849 Views
12 Pages

Crystallinity of Bitumen via WAXD and DSC and Its Effect on the Surface Microstructure

  • Georgios Pipintakos,
  • Hilde Soenen,
  • Bart Goderis,
  • Johan Blom and
  • Xiaohu Lu

24 May 2022

It is well documented that most bituminous binders contain crystallisable material. This crystallisable fraction, often referred to as paraffinic or natural wax, is associated with the bitumen’s origin and has an influence on its rheological pe...

  • Article
  • Open Access
10 Citations
5,786 Views
12 Pages

7 October 2020

In this study, we evaluated the invasion of bacteria into the dentinal tubules of retrofilled extracted human teeth, and the influence of different fluorescently labeled retrograde filling materials on the bacterial invasion and viability, by means o...

  • Article
  • Open Access
11 Citations
2,597 Views
12 Pages

Antilisterial Effectiveness of Origanum vulgare var. hirtum and Coridothymus capitatus Essential Oils and Hydrolates Alone and in Combination

  • Serena D’Amato,
  • Chiara Rossi,
  • Francesca Maggio,
  • Luca Valbonetti,
  • Vincenzo Savini,
  • Antonello Paparella and
  • Annalisa Serio

12 March 2024

The antimicrobial activity of Origanum vulgare var. hirtum (O) and Coridothymus capitatus (C) essential oils (EOs) and hydrolates (HYs) of the same botanical species was evaluated on sixteen L. monocytogenes strains from food and clinical origins. Th...

  • Article
  • Open Access
14 Citations
3,885 Views
11 Pages

The stabilization of probiotics for application in non-refrigerated food products is a challenging task. In the present study, probiotic Lactobacillus paracasei (Lacticaseibacillus paracasei) ATCC 55544 cells were immobilized in a dairy matrix compri...

  • Article
  • Open Access
11 Citations
2,683 Views
20 Pages

Acyclovir (ACV) controls cutaneous herpes, genital herpes, herpes keratitis, varicella zoster, and chickenpox. From previously reported ACV formulations, we continued to explore the permeation behavior of the optimized ACV loaded optimized ethosome (...

  • Article
  • Open Access
3 Citations
2,400 Views
12 Pages

25 July 2024

Membrane surface fouling has always been a critical issue for the long-term operation of polymeric membranes. Therefore, it is crucial to develop new approaches to prevent fouling. While developing new approaches, characterization methods are greatly...

  • Article
  • Open Access
36 Citations
6,431 Views
11 Pages

11 February 2019

The purpose of this study was to compare the penetration ability of calcium silicate root canal sealers and conventional resin-based sealer using confocal laser scanning microscopy (CLSM). A total of 60 recently extracted single-rooted human premolar...

  • Article
  • Open Access
8 Citations
2,512 Views
13 Pages

20 July 2023

A slight alteration of the enamel surface is inevitable upon debonding of orthodontic brackets, adhesive removal, and finishing/polishing. The aim of this in vitro study was to compare two adhesives and three polishing methods by measuring enamel sur...

  • Article
  • Open Access
9 Citations
3,261 Views
14 Pages

Beyond the Limits of Light: An Application of Super-Resolution Confocal Microscopy (sCLSM) to Investigate Eocene Amber Microfossils

  • Dmitry D. Vorontsov,
  • Vasiliy B. Kolesnikov,
  • Elena E. Voronezhskaya,
  • Evgeny E. Perkovsky,
  • Marielle M. Berto,
  • Joseph Mowery,
  • Ronald Ochoa and
  • Pavel B. Klimov

23 March 2023

Amber is known as one of the best sources of fossil organisms preserved with exceptional fidelity. Historically, different methods of imaging have been applied to amber, including optical microscopy and microtomography. These methods are sufficient t...

  • Article
  • Open Access
2 Citations
1,730 Views
19 Pages

The Effect of Mold Flux Wetting Conditions with Varying Crucible Materials on Crystallization

  • Muhammad Anwarul Nazim,
  • Arezoo Emdadi,
  • Todd Sander and
  • Ronald O’Malley

6 March 2025

Understanding mold flux crystallization is essential for assessing heat transfer during steel casting. The complexity of the mold gap presents challenges in identifying the optimal testing method and nucleation type. This study investigates how varia...

  • Article
  • Open Access
10 Citations
8,266 Views
10 Pages

Aerobic granules were cultivated in a sequencing batch reactor (SBR). COD and ammonia nitrogen removal rate were 94% and 99%, respectively. The diameter, settling velocity and SVI10 of granules ranged from 2 to 5 mm, 80 to 110 m/h and about 40 mL/g...

  • Article
  • Open Access
4 Citations
1,499 Views
15 Pages

Optimizing Cement Content in Controlled Low-Strength Soils: Effects of Water Content and Hydration Time

  • Yilian Luo,
  • Liangwei Jiang,
  • Libing Qin,
  • Qiang Luo,
  • David P. Connolly and
  • Tengfei Wang

3 December 2024

The Ethylene Diamine Tetra-acetic Acid (EDTA) titration test is widely used for determining cement content, but its reliability is influenced by the hydration process of cement, which is affected by factors such as water content and hydration time. D...

  • Article
  • Open Access
16 Citations
3,363 Views
15 Pages

Interaction between Fish Skin Gelatin and Pea Protein at Air-Water Interface after Ultrasound Treatment

  • Davide Odelli,
  • Krystalia Sarigiannidou,
  • Alberto Soliani,
  • Rodolphe Marie,
  • Mohammad Amin Mohammadifar,
  • Flemming Jessen,
  • Giorgia Spigno,
  • Mar Vall-llosera,
  • Antonio Fernandes de Carvalho and
  • Federico Casanova
  • + 1 author

23 February 2022

The interaction between fish skin gelatin (FG) and pea protein isolate (PPI) was investigated at the air-water interface (A-W) before and after a high intensity (275 W, 5 min) ultrasound treatment (US). We analyzed the properties of the single protei...

  • Article
  • Open Access
38 Citations
4,450 Views
12 Pages

Green Synthesis of Silver Nanoparticles from Camellia sinensis and Its Antimicrobial and Antibiofilm Effect against Clinical Isolates

  • Syed Ghazanfar Ali,
  • Mohammad Jalal,
  • Hilal Ahmad,
  • Diwakar Sharma,
  • Akil Ahmad,
  • Khalid Umar and
  • Haris Manzoor Khan

8 October 2022

The green synthesis method of was used for the synthesis of silver nanoparticles using Camellia sinensis (green tea). The Camellia sinensis silver nanoparticles (CS-AgNPs) were characterized using different techniques, including UV-Vis (ultra violet-...

  • Article
  • Open Access
6 Citations
2,849 Views
13 Pages

14 March 2023

Gastrotrichs are highly diverse and abundant in all aquatic ecosystems; however, they are often overlooked. During a biodiversity survey in Sardinia (Italy), a new species of gastrotrich herein described was discovered. Specimens of Urodasys bifidost...

  • Article
  • Open Access
14 Citations
3,669 Views
22 Pages

Optimization, In Vitro and Ex Vivo Assessment of Nanotransferosome Gels Infused with a Methanolic Extract of Solanum xanthocarpum for the Topical Treatment of Psoriasis

  • Nilanchala Sahu,
  • Perwez Alam,
  • Asad Ali,
  • Neeraj Kumar,
  • Rama Tyagi,
  • Swati Madan,
  • Ramanpreet Walia and
  • Shikha Saxena

2 February 2024

The goal of this investigation is to improve the topical delivery of medicine by preparing and maximizing the potential of a nanotransferosome gel infused with Solanum xanthocarpum methanolic extract (SXE) to provide localized and regulated distribut...

  • Article
  • Open Access
11 Citations
3,966 Views
13 Pages

Biofilm virulence is mainly based on its bacterial cell surrounding biofilm matrix, which contains a scaffold of exopolysaccharides, carbohydrates, proteins, lipids, and nucleic acids. Targeting these nucleid acids or proteins could enable an efficie...

  • Article
  • Open Access
1 Citations
2,470 Views
13 Pages

17 August 2023

Vital gluten is increasingly researched as a non-food product for biodegradable materials. During processing, the protein network is confronted with increased thermal and mechanical stress, altering the network characteristics. With the prospect of u...

  • Article
  • Open Access
1 Citations
2,269 Views
12 Pages

Structural Studies of Mexican Husk Tomato (Physalis ixocarpa) Fruit Cutin

  • Daniel Arrieta-Baez,
  • Camila Quezada Huerta,
  • Giovana Simone Rojas-Torres,
  • María de Jesús Perea-Flores,
  • Héctor Francisco Mendoza-León and
  • Mayra Beatriz Gómez-Patiño

28 December 2023

Green tomato (Physalis ixocarpa) is a specie native to Mexico, and it is known as “tomatillo” or “husk tomato”. The fruit contains vitamins, minerals, phenolic compounds, and steroidal lactones, presenting antimicrobial activi...

  • Article
  • Open Access
17 Citations
3,291 Views
17 Pages

6 November 2023

In a continuous search for an essential antidiabetic agent, Sida cordifolia hydroalcoholic (SCHA) extract-loaded chitosan nanoparticles (SCHA-CS-NP) were optimized. The Box–Behnken design (BBD Design-Expert software, version 14) with three para...

  • Article
  • Open Access
49 Citations
7,002 Views
14 Pages

The aim of this present work was to prepare triamcinolone acetonide (TA)-loaded nanostructured lipid carriers (TA-loaded NLCs) for buccal drug delivery systems using the Box-Behnken design. A hot homogenization method was used to prepare the TA-loade...

  • Review
  • Open Access
23 Citations
6,275 Views
25 Pages

Traditional Microscopic Techniques Employed in Dental Adhesion Research—Applications and Protocols of Specimen Preparation

  • Agnieszka Nawrocka,
  • Ireneusz Piwonski,
  • Salvatore Sauro,
  • Annalisa Porcelli,
  • Louis Hardan and
  • Monika Lukomska-Szymanska

21 October 2021

Microscopy is a traditional method to perform ex vivo/in vitro dental research. Contemporary microscopic techniques offer the opportunity to observe dental tissues and materials up to nanoscale level. The aim of this paper was to perform a literature...

  • Article
  • Open Access
13 Citations
4,400 Views
18 Pages

Morphology of Neutrophils during Their Activation and NETosis: Atomic Force Microscopy Study

  • Viktoria Sergunova,
  • Vladimir Inozemtsev,
  • Nina Vorobjeva,
  • Elena Kozlova,
  • Ekaterina Sherstyukova,
  • Snezhanna Lyapunova and
  • Aleksandr Chernysh

2 September 2023

Confocal microscopy and fluorescence staining of cellular structures are commonly used to study neutrophil activation and NETosis. However, they do not reveal the specific characteristics of the neutrophil membrane surface, its nanostructure, and mor...

  • Article
  • Open Access
1 Citations
727 Views
38 Pages

24 November 2025

This study presents an integrated digital and archaeometric investigation of the Roman fortress of Sacidava, located in Dobrogea, Romania. Combining 3D digital reconstruction and advanced material analysis, the research explores both the original arc...

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