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  • Article
  • Open Access
8 Citations
3,415 Views
16 Pages

Selective Leaching of Arsenic from Copper Concentrates in Hypochlorite Medium

  • Maria Cecilia Hernández,
  • Oscar Benavente,
  • Antoni Roca,
  • Evelyn Melo and
  • Víctor Quezada

27 October 2023

The selective leaching of arsenic using sodium hypochlorite was evaluated in order to reduce its concentration in a copper concentrate. The best conditions achieved in the synthetic concentrate were applied to the industrial concentrate. First, the i...

  • Article
  • Open Access
457 Views
14 Pages

Surface Characterization of Chalcopyrite Dissolution in Hypochlorite Medium

  • Oscar Benavente,
  • María-Cecilia Hernández,
  • Evelyn Melo,
  • Víctor Quezada,
  • Joan Vignals and
  • Antoni Roca

29 November 2025

The surface of chalcopyrite was studied by XPS characterization for an unleached chalcopyrite, and, after being leached in an alkaline oxidant medium at room temperature, pH 12.5, and [ClO] 0.34 M, the reaction of enargite presented high selec...

  • Article
  • Open Access
8 Citations
3,584 Views
12 Pages

Anti-Hypochlorite and Catalytic Activity of Commercially Available Moringa oleifera Diet Supplement

  • Karolina Starzak,
  • Bernadette Creaven,
  • Arkadiusz Matwijczuk,
  • Alicja Matwijczuk and
  • Dariusz Karcz

12 September 2019

Aiming at the assessment of the pro-health, and especially anti-hypochlorite properties of Moringa oleifera species a representative, commercially available Moringa oleifera dietary supplement was used as a substrate for the preparation of aqueous Mo...

  • Article
  • Open Access
14 Citations
3,791 Views
11 Pages

The Products of Probiotic Bacteria Effectively Treat Persistent Enterococcus faecalis Biofilms

  • Shatha Safadi,
  • Harsh Maan,
  • Ilana Kolodkin-Gal,
  • Igor Tsesis and
  • Eyal Rosen

Objectives: Enterococcus faecalis is a Gram-positive commensal bacterium that possesses various survival and virulence factors, including the ability to compete with other microorganisms, invade dentinal tubules, and resist nutritional deprivation. E...

  • Article
  • Open Access
5 Citations
4,393 Views
18 Pages

Symbiotic Seed Germination and Seedling Development of Epidendrum geminiflorum Knuth from Ecuador

  • Paulina H. Quijia-Lamiña,
  • Luis E. Baquero,
  • Michael E. Kane and
  • Lawrence W. Zettler

8 February 2023

A greater understanding of the relationship between native orchids and their mycorrhizal symbionts is needed to ensure more effective orchid conservation strategies. A protocol for symbiotic seed germination and seedling development was developed for...

  • Article
  • Open Access
12 Citations
3,971 Views
13 Pages

Antibiofilm Properties of Antiseptic Agents Used on Pseudomonas aeruginosa Isolated from Diabetic Foot Ulcers

  • Koko Barrigah-Benissan,
  • Jerome Ory,
  • Catherine Dunyach-Remy,
  • Cassandra Pouget,
  • Jean-Philippe Lavigne and
  • Albert Sotto

24 September 2022

In diabetic foot ulcers (DFUs), biofilm formation is a major challenge that promotes wound chronicity and delays healing. Antiseptics have been proposed to combat biofilms in the management of DFUs. However, there is limited evidence on the activity...

  • Article
  • Open Access
9 Citations
3,238 Views
18 Pages

Optimization of Propagation of the Polish Strain of Aldrovanda vesiculosa in Tissue Culture

  • Marzena Parzymies,
  • Magdalena Pogorzelec and
  • Alicja Świstowska

23 September 2022

Aldrovanda vesiculosa is a rare and critically endangered carnivorous plant species. Its populations have declined worldwide, so there is a need to protect the species from extinction. The research was conducted to establish an effective method of in...

  • Article
  • Open Access
2 Citations
1,446 Views
12 Pages

The Establishment of a Highly Efficient In Vitro Regeneration System for Viburnum opulus L. ‘Roseum’

  • Yajing Ning,
  • Hao Dong,
  • Xinxin Zhang,
  • Yanhua Li,
  • Chengpeng Cui and
  • Shujuan Li

26 January 2025

Viburnum opulus L. ‘Roseum’ is a highly valuable ornamental plant for landscaping, but it has a long propagation cycle and low propagation coefficient. In this study, stem segments with axillary buds from Viburnum opulus L. ‘Roseum&...

  • Article
  • Open Access
1 Citations
2,582 Views
13 Pages

20 October 2022

Embryos of the sunflower moth, Homoeosoma electellum (Hulst), were cryopreserved after modification to the method that was previously described for Pectinophora gossipiella. The workflow to develop the protocol consisted of methods to weaken the embr...

  • Article
  • Open Access
28 Citations
6,765 Views
11 Pages

19 April 2021

Aldrovanda vesiculosa is a carnivorous water plant which is endangered by extinction worldwide. The number of natural stands and populations has decreased; therefore, there is a need for its active protection. The best method would be an in vitro cul...

  • Article
  • Open Access
6 Citations
2,721 Views
19 Pages

Culture Shock: An Investigation into the Tolerance of Pathogenic Biofilms to Antiseptics in Environments Resembling the Chronic Wound Milieu

  • Justyna Paleczny,
  • Malwina Brożyna,
  • Bartłomiej Dudek,
  • Aleksandra Woytoń,
  • Grzegorz Chodaczek,
  • Marta Szajnik and
  • Adam Junka

8 December 2023

Credible assessment methods must be applied to evaluate antiseptics’ in vitro activity reliably. Studies indicate that the medium for biofilm culturing should resemble the conditions present at the site of infection. We cultured S. aureus, S. e...

  • Article
  • Open Access
9 Citations
3,949 Views
13 Pages

Asymbiotic Seed Germination and In Vitro Seedling Development of the Endangered Orchid Species Cypripedium guttatum

  • Hyeong Bin Park,
  • Jiae An,
  • Kee-Hwa Bae,
  • Seung Hyo Hong,
  • Hwan Joon Park,
  • Seongjun Kim,
  • Chang Woo Lee,
  • Byoung-Doo Lee,
  • Ju Hyoung Baek and
  • Jung Eun Hwang
  • + 1 author

7 November 2023

Cypripedium guttatum is a highly restricted terrestrial orchid that faces increasing endangerment owing to its habitat destruction and illegal collection. Compared to epiphytic orchids, terrestrial orchids such as C. guttatum have harder seed coats a...

  • Article
  • Open Access
2 Citations
2,600 Views
11 Pages

Optimizing Green Globular Body Induction for Micropropagation of Microsorum pteropus ‘Windeløv’

  • Wirawan Suwannamali,
  • Kuang-Teng Wang,
  • Chia-Chen Su,
  • Phunsin Kantha,
  • Yuh Tzean and
  • Tsung-Meng Wu

Microsorum pteropus ‘Windeløv’ is a water fern from Asia commonly used in aquarium landscapes. This study aimed to develop a mass production method through the induction of green globular bodies (GGBs) from leaf explants of M. pter...

  • Article
  • Open Access
12 Citations
7,726 Views
12 Pages

The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds

  • Anita Bošnjak Mihovilović,
  • Snježana Kereša,
  • Boris Lazarević,
  • Snježana Topolovec Pintarić,
  • Katarina Martinko,
  • Zvjezdana Marković,
  • Katarina Turkalj and
  • Ivanka Habuš Jerčić

In vitro plants that are free of pathogens are crucial for biotechnological breeding methods. The present study investigates the effects of sterilization with sodium hypochlorite (NaClO) and the addition of Plant Preservative Mixture (PPMTM) to the g...

  • Communication
  • Open Access
9 Citations
6,230 Views
10 Pages

A Complete Micropropagation Protocol for Black-Leaved Zamioculcas zamiifolia (Lodd.) Engl. ‘Dowon’

  • Amirhossein Pourhassan,
  • Behzad Kaviani,
  • Dariusz Kulus,
  • Natalia Miler and
  • Naser Negahdar

Zamioculcas zamiifolia, a drought-resistant plant in the family Araceae, is a popular ornamental potted foliage plant originating from tropical east and subtropical southeast Africa. The growth and propagation rate of this species is low in conventio...

  • Article
  • Open Access
12 Citations
3,955 Views
10 Pages

Aerosol Survival, Disinfection and Formalin Inactivation of Nipah Virus

  • Sophie J. Smither,
  • Lin S. Eastaugh,
  • Lyn M. O’Brien,
  • Amanda L. Phelps and
  • Mark S. Lever

16 September 2022

Nipah virus is a relatively newly discovered emerging virus on the WHO list of priority pathogens which has the potential to cause outbreaks with high fatality rates. Whilst progress is being made in the development of animal models for evaluating va...

  • Article
  • Open Access
3 Citations
2,018 Views
13 Pages

14 January 2025

In this study, an attempt was made to micropropagate the Siberian motherwort (Leonurus sibiricus L.) in in vitro cultures. The aim of this study was to compare methods of sterilization of seeds, which served as a source of initial explants to induce...

  • Article
  • Open Access
1,501 Views
17 Pages

Establishment of an Efficient Regeneration System of Rosa ‘Pompon Veranda’

  • Qin Zhou,
  • Yuexin Zhang,
  • Ruijie Li,
  • Miao Tian,
  • Changlong Zhong,
  • Xiongbo Jiang and
  • Wei Zhang

29 July 2025

Roses are one of the most essential ornamental flowers in the world. At present, traditional techniques such as cross breeding are mainly used in rose breeding. The inefficiency of the in vitro regeneration system has become the limiting step for the...

  • Article
  • Open Access
1 Citations
1,693 Views
14 Pages

29 March 2025

Propagation of hinoki cypress (Japanese cypress, Chamaecyparis obtusa, Cupressaceae) through adventitious bud multiplication was performed using leaf-segment explants from cutting plants of selected adult trees. Explants were successfully surface-ste...

  • Article
  • Open Access
1 Citations
1,224 Views
17 Pages

Induction and Enlargement of Bulblets in Lilium brownii var. viridulum In Vitro

  • Zheng Zhang,
  • Jingfang Wei,
  • Leifeng Xu,
  • Jun Ming and
  • Panpan Yang

18 September 2025

Lilium brownii var. viridulum is a popular natural health product that combines medicine and food. However, the lack of high-quality and efficient propagation technology has been a significant impediment to the development of the L. brownii var. viri...

  • Article
  • Open Access
1 Citations
1,090 Views
15 Pages

Biotic Elicitor-Driven Enhancement of In Vitro Micropropagation and Organogenesis in Solanum tuberosum L. cv. Fianna

  • Mario James-Forest,
  • Ma del Carmen Ojeda-Zacarías,
  • Alhagie K. Cham,
  • Héctor Lozoya-Saldaña,
  • Rigoberto E. Vázquez-Alvarado,
  • Emilio Olivares-Sáenz and
  • Alejandro Ibarra-López

24 September 2025

This study evaluates the impact of biotic elicitors and hormone regimes on the in vitro establishment, shoot multiplication, and organogenesis of Solanum tuberosum L. cv. Fianna under controlled laboratory conditions. Explants derived from pre-treate...

  • Article
  • Open Access
10 Citations
3,806 Views
15 Pages

Electrochemical Conversion of the Lignin Model Veratryl Alcohol to Veratryl Aldehyde Using Manganese(III)-Schiff Base Homogeneous Catalysts

  • Lara Rouco,
  • M. Isabel Fernández-García,
  • Ana M. González-Noya,
  • Gustavo González-Riopedre,
  • Alexei M. Tyryshkin and
  • Marcelino Maneiro

20 August 2019

Lignin and other colored structures need to be bleached after the Kraft process in the pulp industry. Development of environmentally-safe bleaching catalysts or electrocatalysts constitutes an attractive strategy for selective removal of lignin. Seve...

  • Article
  • Open Access
20 Citations
7,325 Views
26 Pages

The High Impact of Staphylococcus aureus Biofilm Culture Medium on In Vitro Outcomes of Antimicrobial Activity of Wound Antiseptics and Antibiotic

  • Justyna Paleczny,
  • Adam Junka,
  • Malwina Brożyna,
  • Karolina Dydak,
  • Monika Oleksy-Wawrzyniak,
  • Daria Ciecholewska-Juśko,
  • Ewelina Dziedzic and
  • Marzenna Bartoszewicz

26 October 2021

The staphylococcal biofilm-based infections of wounds still pose a significant therapeutical challenge. Treated improperly, they increase the risk of limb amputation or even death of the patient. The present algorithms of infected wound treatment inc...

  • Article
  • Open Access
1 Citations
918 Views
14 Pages

In Vitro Propagation Protocol for Porlieria chilensis: Efficient Ex Vitro Rooting and Acclimatization

  • Francesca Guerra,
  • Loreto Badilla,
  • Ricardo Cautín and
  • Mónica Castro

Porlieria chilensis Johnst. (guayacán), an endemic Chilean species native to the sclerophyllous forests, is experiencing a significant population decline. Typically growing as a shrub or small tree on sunny slopes and rocky soils, its populati...

  • Article
  • Open Access
1 Citations
1,165 Views
19 Pages

18 August 2025

Hypolepis punctata, an aromatic fern with insect-resistant and ornamental potential. Up to date, no studies have reported its micropropagation, particularly using vegetative organs as explants. The optimized stolon sterilization (81.11%) employed 75%...

  • Proceeding Paper
  • Open Access
1 Citations
1,401 Views
5 Pages

Dicentra × hybrida is a popular ornamental hybrid, cultivated in parks and gardens worldwide. To date, there are no reports on establishing a plant tissue culture system in this species. This study aims to develop an efficient micropropagation protoc...

  • Article
  • Open Access
16 Citations
7,274 Views
11 Pages

Effects of Vitamin B12 Deficiency on Amyloid-β Toxicity in Caenorhabditis elegans

  • Arif Andra,
  • Shoko Tanigawa,
  • Tomohiro Bito,
  • Atsushi Ishihara,
  • Fumio Watanabe and
  • Yukinori Yabuta

High homocysteine (Hcy) levels, mainly caused by vitamin B12 deficiency, have been reported to induce amyloid-β (Aβ) formation and tau hyperphosphorylation in mouse models of Alzheimer’s disease. However, the relationship between B12 deficiency and A...

  • Article
  • Open Access
1,485 Views
17 Pages

25 April 2025

Selenium has been classified as a strategic element as it is required for the technology and energy industry. It is not found in abundance in the Earth’s crust, which is why about 90% of selenium is obtained from the treatment of anode slimes,...

  • Article
  • Open Access
526 Views
17 Pages

4 January 2026

In the present study, we developed an efficient and reproducible protocol for in vitro regeneration and Agrobacterium tumefaciens-mediated genetic transformation of Broussonetia papyrifera (L.) L’Hér. ex Vent. (paper mulberry) using leaf...

  • Article
  • Open Access
51 Citations
16,371 Views
17 Pages

26 March 2021

‘Bentong’ ginger is the most popular variety of Zingiber officinale in Malaysia. It is vegetatively propagated and requires a high proportion of rhizomes as starting planting materials. Besides, ginger vegetative propagation using its rhizomes is acc...

  • Article
  • Open Access
844 Views
14 Pages

Micropropagation of Quillaja saponaria: A Biotechnological Solution for Conservation and Sustainable Commercial Use of This Endemic Chilean Woody Species

  • Francesca Guerra,
  • Miriam Montecinos,
  • Ingrid Salgado,
  • Javier González,
  • Ricardo Cautín and
  • Mónica Castro

Quillaja saponaria Molina, a tree species endemic to central Chile, is critical to the pharmaceutical and biotechnology industries due to its triterpenic saponins, which exhibit potent immunostimulant, antiviral, and surfactant activities. However, t...

  • Article
  • Open Access
10 Citations
4,646 Views
11 Pages

Feverfew (Tanacetum parthenium) is a well-known multi-functional plant with anti-inflammatory, cardiotonic, antiangiogenic, and anticancer effects. The therapeutic value of this plant is due to its phytochemical constitutes, especially parthenolide....

  • Article
  • Open Access
735 Views
19 Pages

Blackberry (Rubus L.) cultivation in Kazakhstan is constrained by the limited availability of certified planting material and the absence of standardized micropropagation protocols adapted to local conditions. This study aimed to optimize the key sta...

  • Article
  • Open Access
2,997 Views
17 Pages

The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (Prunus canescens × Prunus avium): A Novel Cherry Rootstock

  • Ikra Manzoor,
  • Khalid Mushtaq Bhat,
  • Mohammad Amin Mir,
  • Narendran M. Nair,
  • Aashiq Hussain Pandit,
  • Ume Kulsum,
  • Shoeb Quadri,
  • Smithal Deshmukh and
  • Taras Pasternak

“GiSelA 17” (Prunus canescens × Prunus avium) is a novel cherry clonal rootstock with the ability to bear fruit early and resist replant situations, and it has a high tolerance to the menaces of Prunus dwarf virus (PDV) and Prunus n...

  • Article
  • Open Access
17 Citations
7,160 Views
9 Pages

The Antibacterial Efficacy and In Vivo Toxicity of Sodium Hypochlorite and Electrolyzed Oxidizing (EO) Water-Based Endodontic Irrigating Solutions

  • Sung-Chih Hsieh,
  • Nai-Chia Teng,
  • Chia Chun Chu,
  • You-Tai Chu,
  • Chung-He Chen,
  • Liang-Yu Chang,
  • Chieh-Yun Hsu,
  • Ching-Shuan Huang,
  • Grace Ying-Wen Hsiao and
  • Jen-Chang Yang

7 January 2020

The objective of this study was to evaluate the antibacterial efficacy against Enterococcus faecalis and Streptococcus mutans and in vivo toxicity using embryonic zebrafish assays of sodium hypochlorite (NaOCl) and electrolyzed oxidizing (EO) water (...

  • Feature Paper
  • Article
  • Open Access
16 Citations
3,497 Views
8 Pages

CBCT-Based Assessment of Vapor Lock Effects on Endodontic Disinfection

  • Francesco Puleio,
  • Angelo Sergio Lizio,
  • Valentina Coppini,
  • Roberto Lo Giudice and
  • Giuseppe Lo Giudice

23 August 2023

Background: The phenomenon of vapor lock, characterized by the formation of gas bubbles, poses challenges in achieving effective cleaning and debridement during endodontic treatments. This study aimed to evaluate the presence of vapor lock and to qua...

  • Article
  • Open Access
10 Citations
2,907 Views
14 Pages

Cytotoxic Effects on Gingival Mesenchymal Stromal Cells and Root Surface Modifications Induced by Some Local Antimicrobial Products Used in Periodontitis Treatment

  • Irina Lupșe,
  • Emoke Pall,
  • Lucian Barbu Tudoran,
  • Adriana Elena Bulboacă,
  • Andreea Ciurea,
  • Iulia Cristina Micu,
  • Alexandra Roman,
  • Ada Gabriela Delean,
  • Alexandrina Muntean and
  • Andrada Soancă

3 September 2021

(1) Background: this study aims to test the cytotoxicity of three antimicrobial products used in periodontitis treatment on gingival mesenchymal stem cells (gMSCs) and their influence on root surfaces and gMSC adhesion. We tested the null hypothesis...

  • Article
  • Open Access
6 Citations
3,391 Views
11 Pages

Cytotoxicity and Efficacy in Debris and Smear Layer Removal of HOCl-Based Irrigating Solution: An In Vitro Study

  • Goda Bilvinaite,
  • Ruta Zongolaviciute,
  • Saulius Drukteinis,
  • Virginija Bukelskiene and
  • Elisabetta Cotti

In the present study we evaluated the cytotoxicity of super-oxidized water on human gingival fibroblasts and its efficacy in debris and smear layer removal from root canal walls. Cultured gingival fibroblasts were exposed to super-oxidized water (Ste...

  • Article
  • Open Access
334 Views
18 Pages

Establishment and Shoot Development Responses of Hylocereus undatus Under Plant Growth Regulator Treatments

  • Cristina Pech-Jiménez,
  • Paloma A. Salgado-Mercado,
  • Alejandra Hernandez and
  • Liliana S. Muñoz-Ramírez

14 February 2026

Background/Objectives: Hylocereus undatus is a high-value crop whose conventional propagation is inefficient for commercial scaling. This study aimed to develop an optimized protocol for in vitro establishment and to define optimal plant growth regul...

  • Article
  • Open Access
21 Citations
9,963 Views
10 Pages

Suaeda aralocaspica is an annual halophyte in the Amaranthaceae in the saline deserts of central Asia. This plant has succulent leaves and grape-like fruits and is a potential horticultural plant. To obtain the efficient sterilization method and opti...

  • Article
  • Open Access
36 Citations
7,197 Views
24 Pages

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

  • Han-Yong Park,
  • Kyung-Su Kim,
  • Gunes Ak,
  • Gokhan Zengin,
  • Zoltán Cziáky,
  • József Jekő,
  • Kathalingam Adaikalam,
  • Kihwan Song,
  • Doo-Hwan Kim and
  • Iyyakkannu Sivanesan

5 April 2021

This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of Kaempferia parviflora to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxid...

  • Article
  • Open Access
12 Citations
2,952 Views
15 Pages

27 May 2023

In recent years, there has been growing interest in the application of UV/chlorine advanced oxidation processes for wastewater treatment. However, few studies have investigated this process in a strongly basic medium (pH > 10), which is a common c...

  • Article
  • Open Access
10 Citations
4,302 Views
22 Pages

10 May 2022

Recently, environmental and ecological concerns have become a major issue owing to the shortage of resources, high cost, and so forth. In my research, I present an innovative, environmentally friendly, and economical way to prepare nanocellulose from...

  • Article
  • Open Access
9 Citations
2,534 Views
19 Pages

Innovative Curved-Tip Reactor for Non-Thermal Plasma and Plasma-Treated Water Generation: Synergistic Impact Comparison with Sodium Hypochlorite in Dental Root Canal Disinfection

  • Raúl Arguello-Sánchez,
  • Régulo López-Callejas,
  • Benjamín Gonzalo Rodríguez-Méndez,
  • Rogelio Scougall-Vilchis,
  • Ulises Velázquez-Enríquez,
  • Antonio Mercado-Cabrera,
  • Rosendo Peña-Eguiluz,
  • Raúl Valencia-Alvarado and
  • Carlo Eduardo Medina-Solís

17 November 2023

Non-thermal plasmas (NTPs), known as cold atmospheric plasmas (CAPs), hold great potential for diverse medical applications, including dentistry. However, traditional linear and rigid dielectric barrier discharge reactors used for NTP generation enco...

  • Systematic Review
  • Open Access
4 Citations
2,899 Views
11 Pages

Chitosan’s Ability to Remove the Smear Layer—A Systematic Review of Ex Vivo Studies

  • Ana Ferreira-Reguera,
  • Inês Ferreira,
  • Irene Pina-Vaz,
  • Benjamín Martín-Biedma and
  • José Martín-Cruces

14 January 2025

Background and Objectives: This systematic review aimed to compare the effect of chitosan in smear layer removal with other commonly used chelators during root canal treatment. Materials and Methods: The PRISMA guidelines were followed. Ex vivo studi...

  • Article
  • Open Access
1 Citations
1,818 Views
15 Pages

29 June 2025

Introduction: Successful root canal therapy relies on thorough cleaning and disinfection to eliminate microorganisms and residual pulp tissue. Advanced irrigation activation techniques, including Sonic, Ultrasonic, and Diode Laser activation, have im...