Culture Shock: An Investigation into the Tolerance of Pathogenic Biofilms to Antiseptics in Environments Resembling the Chronic Wound Milieu
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Microorganisms and Culture Conditions
- Staphylococcus aureus ATCC 6538;
- Staphylococcus epidermidis PCM 2118;
- Pseudomonas aeruginosa ATCC 27853;
- Escherichia coli ATCC 25922;
- Candida albicans ATCC 10231.
4.2. Ability of the Strains to Form Biofilms
4.2.1. Biomass Measurement
4.2.2. Metabolic Activity Measurement (Richards Method)
4.2.3. Confocal Microscopy
4.3. Antimicrobial and Antibiofilm Activities of Selected Antimicrobials
4.3.1. Antimicrobials
- Polyhexamethylene biguanide hydrochloride (PHMB)—Prontosan® wound irrigation solution (B. Braun, Melsungen, Germany), containing polyhexamethylene biguanide (0.1%), undecylenamidopropyl betaine (0.1%), and purified water.
- Super-oxidized solution with hypochlorites (SOHs)—Granudacyn® wound irrigation solution (Molnycke, Gothenburg, Sweden) containing water, sodium chloride, hypochlorous acid (0.005%), and sodium hypochlorite (0.005%).
- Povidone-iodine (PVP-I)—Braunol® skin solution liquid (B. Braun, Melsungen, Germany), containing 7.5% of iodized povidone with 10% available iodine, sodium dihydrogen phosphate dihydrate, sodium iodate, macrogol 9 lauryl ether, sodium hydroxide, and purified water.
4.3.2. Minimal Inhibitory Concentration (MIC)
4.3.3. Minimal Biofilm Eradication Concentration (MBEC)
4.3.4. Cellulose-Based Biofilm (CBB) Model
4.4. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Minimal Inhibitory Concentration (mg/L) | ||||||
---|---|---|---|---|---|---|
PHMB | SOHs | PVP-I | ||||
MH | IVWM | MH | IVWM | MH | IVWM | |
S. aureus | 2 | 2 | R | R | 1172 | 937.5 |
S. epidermidis | 0.5 | 0.5 | 5 | R | 586 | 4688 |
E. coli | 2 | 9 | R | R | 2344 | 9375 |
P. aeruginosa | 8 | 63 | R | R | 9375 | 9375 |
C. albicans | 0.5 | 0.5 | R | R | 2344 | 9375 |
Minimal Biofilm Eradication Concentration (mg/L) | ||||||
---|---|---|---|---|---|---|
PHMB | SOHs | PVP-I | ||||
MH | IVWM | MH | IVWM | MH | IVWM | |
S. aureus | 125 | 250 | 0.100 | 100 | 938 | 18,750 |
S. epidermidis | ND | ND | ND | ND | ND | ND |
E. coli | 63 | 125 | 100 | 100 | 9380 | 18,750 |
P. aeruginosa | 500 | 250 | R | R | 18,750 | 18,750 |
C. albicans | 15 | 500 | 100 | R | 9380 | 18,750 |
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Paleczny, J.; Brożyna, M.; Dudek, B.; Woytoń, A.; Chodaczek, G.; Szajnik, M.; Junka, A. Culture Shock: An Investigation into the Tolerance of Pathogenic Biofilms to Antiseptics in Environments Resembling the Chronic Wound Milieu. Int. J. Mol. Sci. 2023, 24, 17242. https://doi.org/10.3390/ijms242417242
Paleczny J, Brożyna M, Dudek B, Woytoń A, Chodaczek G, Szajnik M, Junka A. Culture Shock: An Investigation into the Tolerance of Pathogenic Biofilms to Antiseptics in Environments Resembling the Chronic Wound Milieu. International Journal of Molecular Sciences. 2023; 24(24):17242. https://doi.org/10.3390/ijms242417242
Chicago/Turabian StylePaleczny, Justyna, Malwina Brożyna, Bartłomiej Dudek, Aleksandra Woytoń, Grzegorz Chodaczek, Marta Szajnik, and Adam Junka. 2023. "Culture Shock: An Investigation into the Tolerance of Pathogenic Biofilms to Antiseptics in Environments Resembling the Chronic Wound Milieu" International Journal of Molecular Sciences 24, no. 24: 17242. https://doi.org/10.3390/ijms242417242