Search Results (487)

This study characterizes the chemical defense system of the invasive longhorn beetle Aromia bungii, a destructive pest of Prunus trees, addressing the limited understanding of chemical defensive mechanisms in Cerambycidae. High-speed cameras, environmental scanning electron microscopy (ESEM), dissection, and micro-CT imaging were used to investigate defensive behavior, and the structure of the defense system, in this beetle. Both sexes of A. bungii possess a pair of triangular, sac-like defensive glands symmetrically located in the metathorax, attached to the metasternum. Upon mechanical stimulation, white liquid defensive substances are rapidly ejected through a pair of slit-shaped openings (~200 µm) at the metasternum corners, without gland eversion, reaching over 50 cm. The average weight of substances ejected in first sprays was 7.95 ± 0.79 mg for females and 8.62 ± 2.13 mg for males (mean ± se), with no significant difference between sexes. However, the weight in second sprays after 10 days was significantly lower, at 2.93 ± 0.54 mg for females and 2.22 ± 0.40 mg for males (mean ± se), suggesting that the beetles cannot re-synthesize the substances soon after spray. The weight of ejected substances had no correlation with beetle body weight. Our findings represent the first detailed morphological and functional description of a chemical defense system in Cerambycidae, revealing a specialized metasternal gland and spray mechanism. The substantial but likely non-renewable defensive substances reflect an adaptive trade-off in energy allocation between reproduction and defense in this species that exhibits high fecundity but a short lifespan at the adult stage. Full article

Background: This study investigates the global burden of early-onset bladder cancer (EOBC) from 1990 to 2021, highlighting regional disparities and the growing role of metabolic risk factors. Early-onset bladder cancer (EOBC), diagnosed before age 50, is an emerging global health concern. While less common than kidney cancer, EOBC contributes substantially to mortality and disability-adjusted life years (DALYs), with marked sex disparities. Its global epidemiology remains unassessed systematically. Methods: Using GBD 1990–2021 data, we analyzed EOBC incidence, prevalence, mortality, and DALYs across 204 countries in individuals aged 15–49. Trends were examined via segmented regression, EAPC, and Bayesian age-period-cohort modeling. Inequality was quantified using SII and CI. Decomposition and SDI-efficiency frontier analyses were introduced. Results: From 1990 to 2021, EOBC incidence rose 62.2%, prevalence 73.1%, deaths 15.3%, and DALYs 15.8%. Middle-SDI regions bore the highest burden. Aging drove trends in high-SDI areas and population growth in low-SDI regions. Over 25% of high-SDI countries underperformed in incidence/prevalence control. Smoking remained the leading risk factor, with rising hyperglycemia burdens in high-income areas. Males carried over twice the female burden, peaking at age 45–49. Conclusions: EOBC shows sustained global growth with middle-aged concentration and significant regional disparities. Structural inefficiencies highlight the need for enhanced screening, early warning, and tailored resource allocation. Full article

Background: Eating disorders are increasingly diagnosed in young women, particularly during adolescence. The recently described Anorexic Readiness Syndrome (ARS) is more common than full-blown anorexia. It has been identified in female athletes engaging in disciplines focusing on the aesthetics of the body, in women involved in recreational exercise and in those who are not physically active but strive to achieve the “perfect” figure. The study aimed to assess the severity and prevalence of ARS in women regularly engaging in body-shaping physical activity. Methods: The study included 659 women aged ≥ 14 years who engaged in regular body-shaping physical activity, provided informed consent to participate in the study (in the case of minors, also the consent of a parent or legal guardian), and had no diagnosed chronic diseases. The level of ARS was assessed using a questionnaire measuring attitudes toward food, supplemented with a specially designed survey consisting of 32 questions and a personal data form. Based on the frequency of body-shaping physical activity, participants were divided into two groups: the study group comprised women exercising ≥ 3 times per week (n = 301), while women exercising < 3 times per week constituted the control group (n = 358). The analyses examined the relationships between ARS, frequency of body-shaping physical activity, BMI, and menstrual irregularities. Results: Medium or high ARS level was identified in over 96% (n = 637) of the respondents. The level of ARS was significantly related to the allocation into the group (p = 0.034) and the weekly hours of physical activity (p = 0.011 in the control group; p = 0.020 in the study group). There was a correlation between ARS and menstrual irregularities (p = 0.001). Weak but significant correlations were identified for awareness of eating disorders (V = 0.20; p = 0.001), adherence to a special diet (V = 0.18; p < 0.001) and self-assessed health (V = 0.18; p < 0.001). Conclusions: Higher ARS levels were observed in women reporting greater weekly physical activity. No significant associations were found between ARS and body mass index or body weight. Medium and high ARS levels were significantly associated with self-reported menstrual disturbances, while most participants with elevated ARS were unaware of disordered eating risk. Full article

Background: This study seeks to evaluate the 23 year experience of the İnonu University Liver Transplantation Institute from a public health perspective by examining demographic patterns, etiological factors, and transplantation trends between 2002 and 2025. Aims: This analysis aims to provide insights into the epidemiological landscape of liver transplantation in Türkiye from a public health perspective. Methods: In this retrospective cross sectional study, we analyzed 4011 liver transplant procedures performed between March 2002 and March 2025. Recipient demographics, disease etiologies, donor characteristics, and patients geographic distribution were assessed to delineate regional health needs and service utilization patterns. Results: A total of 4011 patients were included. The cohort comprised 2618 males (65.3%) and 1393 females (34.7%). Recipients were classified as adult (n = 3232, 80.9%) or pediatric (n = 779, 19.1%). Among adults, infectious etiologies were the most prevalent (35.5%), followed by cryptogenic liver cirrhosis (24.7%). In contrast, pediatric patients most commonly presented with toxic etiologies (29.4%), metabolic disorders (22.6%) and bile duct diseases (15.9%). Most liver transplantations were performed using living donors (n = 3481, 86.8%), while deceased donors accounted for 530 procedures (13.2%). Additionally, 244 living donor liver transplantations were performed via liver paired exchange (LPE). Conclusions: These findings may inform resource allocation, health policy development, and the optimization of transplantation services. This center-based model offers a useful framework for characterizing regional health needs and strengthening community health, particularly through prevention, screening, and early intervention strategies for liver diseases. Full article

Background/Objectives: Long COVID (LC) has been linked to fatigue, exercise intolerance, and autonomic dysfunction, but sex-stratified data on cardiovascular responses to maximal exercise—an essential component of personalized medicine—are scarce. This study aimed to examine hemodynamic, autonomic, and functional responses during and up to 24 h after a cardiopulmonary exercise test (CPET) in young adults with and without Long COVID (LC). Methods: In this cross-sectional study, we assessed 38 physically active adults, who were allocated into four subgroups stratified by clinical condition (LC or control) and biological sex: control–female (CON-F; n = 10), LC–female (LC-F; n = 10), control–male (CON-M; n = 10), and LC–male (LC-M; n = 8). Outcomes included systolic (SBP) and diastolic blood pressure (DBP), heart rate (HR), cardiac output (CO), total (TPR) and peripheral vascular resistance (PVR), pulse wave velocity (PWV), augmentation index (AIx@75), and heart rate variability (HF, LF, LF/HF), assessed at rest, peak effort, recovery (1, 3, 5, 10, 30, and 60 min), and through 24 h ambulatory blood pressure monitoring (ABPM) after CPET. Results: SBP increase appropriately during exercise, with higher peaks in males (p < 0.01), and returned to baseline within 5 min across all groups. HR recovery was preserved; however, LC-F showed lower values than CON-F at 3, 5, and 10 min (126 vs. 144 bpm, p = 0.020; 119 vs. 136 bpm, p = 0.020; 94 vs. 109 bpm, p = 0.011), though all groups normalized by 60 min. PWV, AIx@75, TPR and PVR exhibited expected sex-related patterns without LC-related impairments. HRV indices showed transient post-exercise shifts (HF↓, LF↑, LF/HF↑). Ambulatory monitoring confirmed preserved circadian modulation, with normal systolic dipping (11–13%) and no abnormal nocturnal patterns. Conclusions: Young physically active adults with LC showed preserved hemodynamic, autonomic, and vascular responses during and after maximal exercise. These findings contribute to personalized medicine by showing that individualized, sex-stratified cardiovascular assessments reveal no clinically relevant impairments in this population, supporting tailored clinical decision making and exercise prescription. Full article

Two rearing systems are used for Plectropomus leopardus: sea-cage culture and the land-based flow-through aquaculture system. Cages approximate natural conditions and yield many high-quality eggs but offer limited control over ovarian development; the land-based system is highly controllable yet ovaries develop slowly and seldom reach full maturity. We compared these systems by analyzing growth–gonad relationships, monthly hormone profiles (GnRH, E2, GnIH), and hypothalamic transcriptomes in 14- and 18-month-old females. Within each system, body weight did not predict gonadal stage and energy allocation was size-independent. In cages, ovaries reached full maturity with normal histology; in tanks, gonads of all sizes remained at stage III, indicating arrested development. Serum GnRH and E2 displayed parallel increases from 12 to 14 months, declined at 16 months and surged at 18 months in both systems, while GnIH fluctuated inversely, suggesting antagonistic control. Transcriptome analysis identified fshr, cyp11a1 and sox17 as key down-regulated genes in tank-reared fish. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment implicated GnRH, oxidative phosphorylation, ribosome and Wnt pathways in ovarian progression. These findings elucidate reproductive constraints under artificial conditions and provide molecular targets for controllable breeding of P. leopardus. Full article

Within the context of China’s green economy aimed at sustainable development, research on the linkage between water resources and industry has garnered considerable attention in the academic community. However, the impact of total body water (TBW) transfer and allocation embodied in the labor force—the primary economic actors—has not been addressed in the economic sector. On methodology, the “EEIO-SDA-SPD-II” (ISSI) model employed in this study encompasses measurements methods, such as an environmentally extended input–output model (EEIO), structural decomposition analysis (SDA), structural path decomposition (SPD), and the imbalance index (II), to explore the crucial paths, driving factors, and distribution of water transfer in TWB spanning 15 Chinese industries between 2007 and 2022. The findings indicate that the shifts in TBW in the manufacturing sector are more discernible when viewed through the lens of social driving factors. The construction business exhibits the most significant increase in male total body water (MTBW), whereas the education sector reflects the rapid growth in female total body water (FTBW). Pertaining to final demand, domestic consumption constitutes the primary contributor category to the increase in TWB, followed by fixed capital formation and exports. According to the SPD results, the construction sector exerts the greatest influence on the transfer of MTBW, while the education sector is characterized by the highest path coefficient value for FTBW. In contrast, the manufacturing sector shows the most pronounced initial path. Based on the imbalance index analysis, agriculture derives the greatest economic gains from TBW input, whereas the education sector yields the lowest. Full article

The search for natural alternatives to in-feed antibiotics is a central focus in sustainable poultry production. This study investigated the individual and combined effects of Sanguinarine (SA) and Achyranthes bidentata polysaccharides (ABPS), two bioactive compounds derived from Chinese herbs, on the growth performance and immune function of yellow-feathered broilers. A total of 1728 one-day-old female broilers were randomly allocated to 36 treatment groups in a 6 × 6 factorial arrangement, with factors being six levels of SA (0, 0.4, 0.5, 0.6, 0.7, and 0.75 mg/kg) and six levels of ABPS (0, 200, 300, 400, 500, and 600 mg/kg). Over an 8-week period, growth performance indicators, including average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR), were monitored. Immune status was assessed through relative weights of immune organs (thymus, bursa of Fabricius, spleen) and lymphocyte proliferation at 4 and 8 weeks of age. The results demonstrated that SA supplementation significantly improved ADG during the initial 0–2-week period, while its effects on ADFI and FCR varied across different growth stages. ABPS supplementation was most effective in reducing FCR throughout the experiment, with the optimal effect observed at 500 mg/kg. Regarding immune parameters, SA at 0.7 mg/kg yielded the highest relative immune organ weights and lymphocyte proliferation at 4 weeks, whereas 0.5 mg/kg was most effective for lymphocyte proliferation at 8 weeks. ABPS at 500 mg/kg consistently promoted immune organ development and lymphocyte proliferation. A significant synergistic interaction between SA and ABPS was identified for several key outcomes. This interaction influenced ADG and ADFI at 8 weeks of age, relative thymus weight at 4 weeks, and lymphocyte proliferation at both 4 and 8 weeks. Notably, the combination of SA at 0.5 mg/kg and ABPS at 300 mg/kg (A₃B₃) produced a marked enhancement in lymphocyte proliferation. In conclusion, dietary supplementation with SA, ABPS, or their combination can modulate growth performance and enhance immune function in yellow-feathered broilers. The effects are dose-dependent and can be synergistic, with the combination of 0.5 mg/kg SA and 300 mg/kg ABPS showing particular promise for boosting cell-mediated immunity. These findings support the potential of SA and ABPS as beneficial natural feed additives for antibiotic-free poultry production. Full article

To meet market supply and demand, producers must accurately schedule processing dates to ensure optimal pricing. This study developed a practical feeding program system for local Taiwanese chicken breeds, including Guzao males, Huangjin females, and Red Junglefowl males. The system integrates daily predictions of cage-level body weight to guide each flock toward a target weight before the planned processing date. Four prediction models were evaluated, including random forest, XGBoost, Extra Trees, and an artificial neural network. The best-performing model was embedded into the system, and an Extra Trees model was used to estimate the total remaining ration and update daily feed allocations under standard feeding conditions. A validation experiment was conducted using a 54-day batch of Guzao males, during which cage-level data were collected. The feed conversion ratio of birds managed under the feeding program was compared with that of conventional feeding. The results provide preliminary support for the feasibility of a data-guided feeding program system with potential agricultural application value, although additional batches and cross-farm evaluations are needed to confirm generalizability and operational performance. Full article

Objective: This study aimed to investigate annual and seasonal trends, as well as regional differences, in female breast cancer mortality in Hungary between 2004 and 2023. Methods: Data on cancer mortality were obtained from the publicly available nationwide population register. Poisson and quasi-Poisson regression models were applied to investigate the annual trend in breast cancer mortality rates. Cyclic trends in mortality were analysed using the Walter–Elwood method, and regional differences in age-standardised mortality rates (ASMRs) were evaluated across Hungarian regions. Results: Over the two decades studied, a total of 42,779 deaths from breast cancer were recorded. A significant declining trend in annual ASMRs for female breast cancer was observed during the study period (IRR = 0.996; 95% CI [0.993–0.998]; p = 0.002). Seasonal analysis revealed a significant cyclic pattern, with the highest number of deaths occurring during the winter months, peaking in December. The highest age-standardised breast cancer mortality rate (43.9 ± 0.2 per 100,000 female persons per year) was observed in the Capital region (Budapest), while the lowest ASMR (36.2 ± 0.25 per 100,000 female persons per year) was found in the Northern Great Plain region (p = 0.028). Conclusions: Although Hungary has implemented a free national breast cancer screening programme, this study demonstrates that breast cancer mortality remains high in the country. Additionally, breast cancer mortality exhibits significant regional and seasonal variation. These findings underscore the need for targeted public health interventions and optimised resource allocation to improve outcomes. Full article

Background/Objectives: Globally, multiple sclerosis (MS) management is associated with substantial economic burden, but its impact in Saudi Arabia has not been fully quantified. The aim of this study was to estimate the direct medical costs of MS in Saudi Arabia and identify its main cost drivers from a public healthcare payer perspective. Methods: A retrospective, prevalence-based, single-center cost-of-illness analysis was conducted between 2019 and 2020. A bottom-up micro-costing approach was used to estimate the annual direct medical costs. Descriptive and inferential statistics were used. Results: A total of 193 patients with MS were included (mean age, 33 years; 62% female; 66% with relapsing-remitting MS). Overall, 48% of patients were at a mild disease stage, and 57% had a disease duration of less than five years. The total cost incurred during the study period was $4,157,436. The annual direct medical cost per patient is $21,541 ± 1475. Costs were significantly associated with EDSS score and frequent relapses, resulting in an increase of $729 and $1349, respectively. Additionally, disease-modifying therapies were identified as a major cost driver (74%). Conclusions: MS poses a significant financial burden on the Saudi healthcare system. The results of this study can inform policy development and guide resource allocation in planning healthcare services for patients with MS. Full article

Ischemic heart disease (IHD) remains the predominant cause of global mortality, necessitating accurate incidence forecasting for effective prevention strategies. Existing statistical models inadequately capture nonlinear epidemiological patterns, while deep learning approaches lack clinical interpretability. We constructed an interpretable predictive framework combining particle swarm optimization (PSO), bidirectional long short-term memory (BiLSTM) networks, and a novel multi-scale attention mechanism. Age-standardized incidence rates (ASIRs) from the Global Burden of Disease (GBD) 2021 database (1990–2021) were stratified across 24 sex-age subgroups and processed through 10-year sliding windows with advanced feature engineering. SHapley Additive exPlanations (SHAP) provided a three-level interpretability analysis (global, local, and component). The framework achieved superior performance metrics: mean absolute error (MAE) of 0.0164, root mean squared error (RMSE) of 0.0206, and R² of 0.97, demonstrating a 93.96% MAE reduction compared to ARIMA models and a 75.99% improvement over CNN–BiLSTM architectures. SHAP analysis identified females aged 60–64 years and males aged 85–89 years as primary predictive contributors. Architectural analysis revealed the residual connection captured 71.0% of the predictive contribution (main trends), while the BiLSTM–Attention pathway captured 29.0% (complex nonlinear patterns). This interpretable framework transforms opaque algorithms into transparent systems, providing precise epidemiological evidence for public health policy, resource allocation, and targeted intervention strategies for high-risk populations. Full article

Estrogen deficiency is a primary cause of osteoporosis, compromising bone mineral density that may impair peri-implant healing. Given the compromised bone environment associated with estrogen deficiency, strategies such as particle reduction via sonochemistry are promising approaches to enhance regenerative outcomes. However, its effects in promoting bone formation remain insufficiently explored. Therefore, this study evaluated the potential of two sonicated biomaterials to improve peri-implant repair in ovariectomized rats. Fifty female rats were allocated into five groups: blood clot (CLOT), Biogran^® (BGN), sonicated Biogran^® (BGS), Bio-Oss^® (BON), and sonicated Bio-Oss^® (BOS). Tibial peri-implant defects were created 30 days after ovariectomy and analyzed 28 days later by removal torque, microcomputed tomography, and confocal microscopy. BGS exhibited the highest removal torque (6.28 Ncm), followed by BON (5.37 Ncm), BOS (3.92 Ncm), BGN (3.15 Ncm), and CLOT (2.58 Ncm). Micro-CT revealed bone volume fraction (BV/TV) values of 8.07% (CLOT), 6.47% (BOS), 6.02% (BGS), 5.55% (BGN), and 2.84% (BON). For the trabecular number (Tb.N), BGS (1.11 mm⁻¹) showed a significant increase compared with BGN (0.69 mm⁻¹), p < 0.05. These findings show that sonochemically modified bioactive glass improves mechanical stability and trabecular microarchitecture under estrogen-deficient conditions. However, further studies are needed to standardize sonication parameters for different biomaterials and expand their translational applicability. Full article

Background: This study aimed to examine the immediate effects of the Nordic Hamstring Exercise (NHE) on the stiffness of the biceps femoris (BF) and semitendinosus (ST) muscles. Methods: This parallel-group randomised controlled trial followed CONSORT 2025 guidelines. Twenty-four physically active adults (16 females, 8 males) were randomly assigned to an NHE group (n = 12) or a control group (n = 12) using 1:1 gender-stratified randomisation. The NHE group performed 3 sets of 10 repetitions of the NHE, while the control group remained inactive. Muscle stiffness of the BF and ST was assessed pre- and post-intervention using the MyotonPro device. Results: No significant overall changes in hamstring stiffness were observed within or between groups (p > 0.05). Within the NHE group, the ST tended to increase in stiffness (11.25 N/m, p = 0.057), while the BF showed a small, non-significant reduction (−12.00 N/m, p = 0.696). The difference in changes between BF and ST was significant (p = 0.039). Independent of group allocation, males demonstrated significantly higher baseline stiffness than females for BF (258.13 vs. 195.81 N/m, p < 0.001) and for ST (247.88 vs. 174.00 N/m, p = 0.003). Regression analysis showed that only height predicted the change in ST stiffness after NHE (R = 0.625, R² = 0.39, p = 0.030). Conclusions: A single NHE session did not alter overall hamstring stiffness but produced opposite, muscle-specific effects. More research with larger, uniform samples is needed to confirm these findings. Full article

Background: Individuals with severe disability require intensive and long-term healthcare, rehabilitation and social support. Updated population data are essential to inform planning and resource allocation. This study aimed to quantify—with a cross-sectional analysis conducted in 2025—the demographic, clinical, socioeconomic, and geographic characteristics of individuals with severe disability within the Provincial Health Authority (ASP) of Catania (Sicily) in Italy, and to identify statistically significant differences across subgroups. Methods: A cross-sectional analysis was conducted on 3277 individuals officially certified as having severe disability under the Italian Ministerial Decree of 26 September 2016. Data were extracted from administrative records and stratified by age, sex, clinical classification, income level, and healthcare district. Associations were tested using chi-square statistics. Results: Participants had a mean age of 39.14 ± 28.64 years; Minors represented 33% of the disability cohort (vs. 19.4% minors in the general provincial population) with a mean age 10.28 ± 3.55. Adults accounted for 67% of the cohort (vs. 81% adults in the general population), with a mean age of 69.94 ± 24.61 years. Females constituted 43% of the sample (compared with 51% females in the provincial population), whereas males represented 57% (vs. 49% males in the general population). Most individuals (95.9% of the cohort) had an income level below €25,000/year. Conclusions: The study reveals substantial demographic, socioeconomic, and clinical heterogeneity among individuals with severe disability and highlights significant district-level disparities. Notably, minors appear markedly over-represented in the disability cohort compared with the general population, while females are under-represented, indicating potential age- and sex-related differences in disability burden, access to assessment, or underlying diagnostic patterns. These findings indicate the need for stratified, district-sensitive planning approaches, ensuring equitable access to services and optimizing allocation of healthcare and social resources. Full article