Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious Idesia polycarpa Maxim Leaves during Reproductive Stages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Sample Acquisition
2.3. Determination of Leaf Nutrients and Analysis
2.4. Determination of Endophytic Bacteria in Leaves
2.4.1. Total DNA Extraction, PCR Amplification, and Sequencing
2.4.2. Diversity Analysis of Endophytic Bacteria
2.5. Determination of Metabolites in Leaves
2.5.1. Metabolite Extraction
2.5.2. The UPLC–MS/MS Analysis
2.6. Data Analysis
3. Results
3.1. Leaf Nutrient Characteristics in Different Periods
3.2. Differences of Endophytes in Leaves at Different Stages
3.2.1. OTU Distribution and Alpha Diversity
3.2.2. Phylum Level Analysis of Leaf Endophytic Bacteria Community
3.2.3. Genus Level Analysis of Leaf Endophytic Bacteria Community
3.2.4. Difference Analysis of Endophytes in Leaves
3.3. Leaf Metabolite Difference
3.3.1. Sample Principal Component Analysis
3.3.2. Screening of Differential Metabolites in Leaves
3.3.3. Metabolic Pathway Analysis
3.4. Correlation Analysis of Leaf Nutrients with the Microbial Community and Differential Metabolites
4. Discussion
4.1. Nutrient Contents in Idesia Polycarpa Leaves
4.2. Endophytes in Idesia Polycarpa Leaves
4.3. Metabolites in Idesia polycarpa Leaves
4.4. Leaf Nutrients with the Microbial Community and Differential Metabolites
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Period | Sexuality | pH | Total Carbon g·kg−1 | Total Nitrogen g·kg−1 | Total Phosphorus g·kg−1 | Total Potassium g·kg−1 |
---|---|---|---|---|---|---|
May | CS | 5.70 ± 0.48 a | 551.17 ± 43.57 a | 34.15 ± 0.63 a | 4.60 ± 0.17 a | 10.78 ± 1.10 a |
XS | 5.73 ± 0.53 a | 521.20 ± 13.67 a | 29.32 ± 1.89 b | 5.13 ± 0.1 a | 8.80 ± 1.34 a | |
July | CS | 5.67 ± 0.04 a | 446.13 ± 3.53 a | 18.40 ± 1.98 a | 4.37 ± 0.41 a | 9.90 ± 2.98 a |
XS | 5.72 ± 0.09 a | 439.39 ± 4.55 a | 17.62 ± 2.14 a | 3.17 ± 0.33 a | 3.08 ± 0.58 b | |
October | CS | 6.15 ± 0.03 a | 489.20 ± 12.53 a | 24.84 ± 2.57 a | 6.82 ± 0.42 a | 5.24 ± 0.03 a |
XS | 5.97 ± 0.05 b | 492.86 ± 2.89 a | 23.83 ± 2.21 a | 7.96 ± 0.54 a | 4.56 ± 0.39 a |
Samples | Goods Coverage | Shannon | Simpson | ACE Richness |
---|---|---|---|---|
CS5 | 1.00 ± 0.00 a | 1.44 ± 0.6 bc | 0.30 ± 0.06 c | 197.38 ± 144.84 a |
XS5 | 1.00 ± 0.00 a | 0.93 ± 0.06 c | 0.27 ± 0.01 c | 55 ± 13.05 a |
CS10 | 1.00 ± 0.00 a | 5.18 ± 0.17 a | 0.92 ± 0.01 a | 157 ± 6.03 a |
XS10 | 1.00 ± 0.00 a | 3.51 ± 1.23 ab | 0.69 ± 0.12 b | 236.67 ± 78.56 a |
Samples | Goods Coverage | Shannon | Simpson | ACE Richness |
---|---|---|---|---|
CS5 | 1.00 ± 0.00 a | 5.12 ± 0.25 a | 0.95 ± 0.01 a | 67.33 ± 1.45 ab |
XS5 | 1.00 ± 0.00 a | 5.14 ± 0.57 a | 0.95 ± 0.02 a | 105.33 ± 25.67 a |
CS10 | 1.00 ± 0.00 a | 3.85 ± 0.59 a | 0.82 ± 0.09 a | 67 ± 13.53 ab |
XS10 | 1.00 ± 0.00 a | 4.53 ± 0.14 a | 0.92 ± 0.02 a | 49.33 ± 3.33 b |
Comparison Group | Total Number of Differential Metabolites | VIP Value Top Five Metabolites | Log2 FC | p-Value | VIP | Metabolite Types |
---|---|---|---|---|---|---|
CS5 vs. XS5 | 108 | Cyclo (l-phe-l-pro) | 9.671 | 0.000 | 1.288 | Amino acids and derivatives |
2-Phenylethyl beta-d-glucopyranoside | 13.186 | 0.001 | 1.288 | Phenolic acids | ||
Salireposide | 10.385 | 0.002 | 1.288 | Phenolic acids | ||
4-O-(6′-O-glucosyl-p-coumaroyl)-4-hydroxybenzyl alcohol | 3.356 | 0.000 | 1.288 | Phenolic acids | ||
4-Ethoxyphenol | 11.342 | 0.005 | 1.288 | Phenolic acids | ||
CS10 vs. XS10 | 87 | 3,4-Dimethoxyphenol | 10.558 | 0.004 | 1.346 | Phenolic acids |
8-Hydroxyguanosine | 11.931 | 0.004 | 1.346 | Nucleotides and derivatives | ||
Salireposide | −10.336 | 0.005 | 1.346 | Phenolic acids | ||
Diethyl phosphate | 9.431 | 0.004 | 1.346 | Organic acids | ||
Cordycepin (3′-Deoxyadenosine) | 10.013 | 0.005 | 1.346 | Nucleotides and derivatives | ||
CS5 vs. CS10 | 245 | 1-Stearidonoyl-glycerol | −15.663 | 0.000 | 1.175 | Lipids |
Cyclo(l-phe-l-pro) | 8.051 | 0.000 | 1.175 | Amino acids and derivatives | ||
4-Ethoxyphenol | 12.138 | 0.001 | 1.175 | Phenolic acids | ||
Coniferaldehyde | −12.447 | 0.001 | 1.175 | Phenolic acids | ||
Benzoic acid | 11.595 | 0.001 | 1.175 | Phenolic acids | ||
XS5 vs. XS10 | 254 | 1-Stearidonoyl-glycerol | −12.498 | 0.000 | 1.166 | Lipids |
4-(3,4,5-Trihydroxybenzoxy) benzoic acid | 3.737 | 0.000 | 1.166 | Phenolic acids | ||
1,18-Octadecanediol | −10.018 | 0.001 | 1.166 | Lipids | ||
9,12-Octadecadien-6-ynoic acid | −11.759 | 0.002 | 1.166 | Lipids | ||
4-Hydroxy-3-methoxymandelate | −14.586 | 0.003 | 1.166 | Organic acids |
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Feng, J.; Rana, S.; Liu, Z.; Wang, Y.; Cai, Q.; Geng, X.; Zhou, H.; Zhang, T.; Wang, S.; Xue, X.; et al. Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious Idesia polycarpa Maxim Leaves during Reproductive Stages. Life 2022, 12, 2041. https://doi.org/10.3390/life12122041
Feng J, Rana S, Liu Z, Wang Y, Cai Q, Geng X, Zhou H, Zhang T, Wang S, Xue X, et al. Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious Idesia polycarpa Maxim Leaves during Reproductive Stages. Life. 2022; 12(12):2041. https://doi.org/10.3390/life12122041
Chicago/Turabian StyleFeng, Jian, Sohel Rana, Zhen Liu, Yanmei Wang, Qifei Cai, Xiaodong Geng, Huina Zhou, Tao Zhang, Shasha Wang, Xiaoyan Xue, and et al. 2022. "Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious Idesia polycarpa Maxim Leaves during Reproductive Stages" Life 12, no. 12: 2041. https://doi.org/10.3390/life12122041