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4,538 Results Found

  • Article
  • Open Access
38 Citations
9,529 Views
10 Pages

20 August 2012

PAirwise Sequence Comparison (PASC) is a tool that uses genome sequence similarity to help with virus classification. The PASC tool at NCBI uses two methods: local alignment based on BLAST and global alignment based on Needleman-Wunsch algorithm. It...

  • Article
  • Open Access
13 Citations
8,512 Views
16 Pages

28 July 2021

Picobirnaviruses (PBVs) are small, double stranded RNA viruses with an ability to infect a myriad of hosts and possessing a high degree of genetic diversity. PBVs are currently classified into two genogroups based upon classification of a 200 nt sequ...

  • Article
  • Open Access
19 Citations
4,998 Views
16 Pages

Interlaboratory Comparison Study on Ribodepleted Total RNA High-Throughput Sequencing for Plant Virus Diagnostics and Bioinformatic Competence

  • Yahya Z. A. Gaafar,
  • Marcel Westenberg,
  • Marleen Botermans,
  • Krizbai László,
  • Kris De Jonghe,
  • Yoika Foucart,
  • Luca Ferretti,
  • Denis Kutnjak,
  • Anja Pecman and
  • Heiko Ziebell
  • + 6 authors

12 September 2021

High-throughput sequencing (HTS) technologies and bioinformatic analyses are of growing interest to be used as a routine diagnostic tool in the field of plant viruses. The reliability of HTS workflows from sample preparation to data analysis and resu...

  • Article
  • Open Access
5 Citations
3,046 Views
28 Pages

25 November 2022

Parvalbumins are small molecules with important functions in Ca2+ signaling, but their sequence comparisons to date, especially in fish, have been relatively poor. We here, characterize sequence motifs that distinguish parvalbumin subfamilies across...

  • Article
  • Open Access
5 Citations
4,668 Views
11 Pages

7 June 2017

This study tends to assess implicit and explicit types of motor learning in patients with Multiple Sclerosis (MS) and normal peers by means of a serial reaction time. Sample size was 15 for each group and Samples included 30 patients with MS and 30 n...

  • Article
  • Open Access
8 Citations
5,695 Views
15 Pages

Complete Whole Genome Sequences of Escherichia coli Surrogate Strains and Comparison of Sequence Methods with Application to the Food Industry

  • Dustin A. Therrien,
  • Kranti Konganti,
  • Jason J. Gill,
  • Brian W. Davis,
  • Andrew E. Hillhouse,
  • Jordyn Michalik,
  • H. Russell Cross,
  • Gary C. Smith,
  • Thomas M. Taylor and
  • Penny K. Riggs

In 2013, the U.S. Department of Agriculture Food Safety and Inspection Service (USDA-FSIS) began transitioning to whole genome sequencing (WGS) for foodborne disease outbreak- and recall-associated isolate identification of select bacterial species....

  • Article
  • Open Access
3 Citations
2,365 Views
10 Pages

31 October 2024

Next-generation sequencing (NGS) for HIV drug resistance (DR) testing has an increasing number of applications for the detection of low-abundance drug-resistant variants (LA-DRVs) in regard to its features as a quasi-species. However, there is less i...

  • Article
  • Open Access
2 Citations
2,936 Views
24 Pages

Sequence–structure alignment for protein sequences is an important task for the template-based modeling of 3D structures of proteins. Building a reliable sequence–structure alignment is a challenging problem, especially for remote homolog...

  • Article
  • Open Access
22 Citations
12,837 Views
20 Pages

17 March 2015

A tripartite comparison of Archaea phylogeny and taxonomy at and above the rank order is reported: (1) the whole-genome-based and alignment-free CVTree using 179 genomes; (2) the 16S rRNA analysis exemplified by the All-Species Living Tree with 366 a...

  • Article
  • Open Access
1,756 Views
15 Pages

Genomic Analysis of a Novel Torradovirus “Rehmannia Torradovirus Virus”: Two Distinct Variants Infecting Rehmannia glutinosa

  • Yanhong Qin,
  • Shuhao Lu,
  • Yi Wen,
  • Shaojian Li,
  • Suxia Gao,
  • Desheng Zhang,
  • Xuemeng Li,
  • Jin Yang,
  • Li Gu and
  • Chuantao Lu
  • + 2 authors

Rehmannia glutinosa, a crucial medicinal plant native to China, is extensively cultivated across East Asia. We used high-throughput sequencing to identify viruses infecting R. glutinosa with mosaic, leaf yellowing, and necrotic symptoms. A novel Torr...

  • Article
  • Open Access
1 Citations
1,912 Views
19 Pages

Unexpected Genetic Diversity of Nostocales (Cyanobacteria) Isolated from the Phyllosphere of the Laurel Forests in the Canary Islands (Spain)

  • Nereida M. Rancel-Rodríguez,
  • Nicole Sausen,
  • Carolina P. Reyes,
  • Antera Martel Quintana,
  • Barbara Melkonian and
  • Michael Melkonian

A total of 96 strains of Nostocales (Cyanobacteria) were established from the phyllosphere of the laurel forests in the Canary Islands (Spain) and the Azores (Portugal) using enrichment media lacking combined nitrogen. The strains were characterized...

  • Article
  • Open Access
42 Citations
13,566 Views
34 Pages

Information Theory in Computational Biology: Where We Stand Today

  • Pritam Chanda,
  • Eduardo Costa,
  • Jie Hu,
  • Shravan Sukumar,
  • John Van Hemert and
  • Rasna Walia

6 June 2020

“A Mathematical Theory of Communication” was published in 1948 by Claude Shannon to address the problems in the field of data compression and communication over (noisy) communication channels. Since then, the concepts and ideas developed...

  • Article
  • Open Access
3 Citations
3,385 Views
19 Pages

Genomic Diversity among Actinomyces naeslundii Strains and Closely Related Species

  • Sadaf Rasheed Mughal,
  • Sadia Ambreen Niazi,
  • Thuy Do,
  • Steven C. Gilbert,
  • Xavier Didelot,
  • David R. Radford and
  • David Beighton

The aim of this study was to investigate and clarify the ambiguous taxonomy of Actinomyces naeslundii and its closely related species using state-of-the-art high-throughput sequencing techniques, and, furthermore, to determine whether sub-cluste...

  • Article
  • Open Access
16 Citations
3,141 Views
22 Pages

Ranaviruses are promiscuous pathogens that threaten lower vertebrates globally. In the present study, two ranaviruses (SCRaV and MSRaV) were isolated from two fishes of the order Perciformes: mandarin fish (Siniperca chuatsi) and largemouth bass (Mic...

  • Article
  • Open Access
17 Citations
4,685 Views
15 Pages

Sequence Comparison of Vaginolysin from Different Gardnerella Species

  • Erin M. Garcia,
  • Myrna G. Serrano,
  • Laahirie Edupuganti,
  • David J. Edwards,
  • Gregory A. Buck and
  • Kimberly K. Jefferson

20 January 2021

Gardnerella vaginalis has recently been split into 13 distinct species. In this study, we tested the hypotheses that species-specific variations in the vaginolysin (VLY) amino acid sequence could influence the interaction between the toxin and vagina...

  • Article
  • Open Access
10 Citations
3,231 Views
13 Pages

Development of an Online Genome Sequence Comparison Resource for Bacillus cereus sensu lato Strains Using the Efficient Composition Vector Method

  • Kui Wang,
  • Changlong Shu,
  • Alejandra Bravo,
  • Mario Soberón,
  • Hongjun Zhang,
  • Neil Crickmore and
  • Jie Zhang

12 June 2023

An automated method was developed for differentiating closely related B. cereus sensu lato (s.l.) species, especially biopesticide Bacillus thuringiensis, from other human pathogens, B. anthracis and B. cereus sensu stricto (s.s.). In the current res...

  • Article
  • Open Access
1,010 Views
19 Pages

Development and Implementation of a Pilot Intent Recognition Model Based on Operational Sequences

  • Xiaodong Mao,
  • Lishi Ding,
  • Xiaofang Sun,
  • Liping Pang,
  • Ye Deng and
  • Xin Wang

29 August 2025

With the advancement of intelligent human–computer interaction (IHCI) technology, the accurate recognition of an operator’s intent has become essential for improving the collaborative efficiency in complex tasks. To address the challenges...

  • Article
  • Open Access
1 Citations
2,500 Views
16 Pages

Structural Similarities, in Relation with the Cross-Reactivity, of Hymenoptera Allergenic Dipeptidyl Peptidases IV—An Overall Comparison Including a New Dipeptidyl Peptidase IV Sequence from Vespa velutina

  • Rafael I. Monsalve,
  • Manuel Lombardero,
  • Lars H. Christensen,
  • Beatriz Núñez-Acevedo,
  • David González-de-Olano,
  • Miriam Sobrino-García,
  • Rosita M. Castillo-Loja,
  • Susana B. Bravo,
  • Manuela Alonso-Sampedro and
  • Carmen Vidal

14 November 2023

(1) Background: Dipeptidyl Peptidases IV (DPPIVs), present in many organisms, are minor components in the venoms of Hymenoptera, where they have been identified as cross-reactive allergenic molecules. Considering that the structure of homologous DPPI...

  • Article
  • Open Access
28 Citations
3,838 Views
17 Pages

A Sequence Variation in GmBADH2 Enhances Soybean Aroma and Is a Functional Marker for Improving Soybean Flavor

  • Linlin Qian,
  • Hangxia Jin,
  • Qinghua Yang,
  • Longming Zhu,
  • Xiaomin Yu,
  • Xujun Fu,
  • Man Zhao and
  • Fengjie Yuan

The vegetable soybean (Glycine max L. Merr.) plant is commonly consumed in Southeast Asian countries because of its nutritional value and desirable taste. A “pandan-like” aroma is an important value-added quality trait that is rarely foun...

  • Article
  • Open Access
8 Citations
4,491 Views
12 Pages

The Plastid Genome of Deschampsia cespitosa (Poaceae)

  • Jorge O. Chiapella,
  • Michael H. J. Barfuss,
  • Zhi-Qing Xue and
  • Josef Greimler

9 January 2019

Plastid genome analysis of non-model organisms provides valuable information for basic research e.g., molecular evolutionary genomics, phylogeny and phylogeography. Deschampsia cespitosa is the most widespread species of the genus and it is a common...

  • Article
  • Open Access
3 Citations
4,174 Views
16 Pages

ExpressHeart: Web Portal to Visualize Transcriptome Profiles of Non-Cardiomyocyte Cells

  • Gang Li,
  • Changfei Luan,
  • Yanhan Dong,
  • Yifang Xie,
  • Scott C. Zentz,
  • Rob Zelt,
  • Jeff Roach,
  • Jiandong Liu,
  • Li Qian and
  • Yuchen Yang
  • + 1 author

19 August 2021

Unveiling the molecular features in the heart is essential for the study of heart diseases. Non-cardiomyocytes (nonCMs) play critical roles in providing structural and mechanical support to the working myocardium. There is an increasing amount of sin...

  • Article
  • Open Access
1 Citations
1,479 Views
17 Pages

25 November 2024

The locations of hot pulsating variables in the H–R diagram are found using the effective temperatures derived from spectroscopic analysis and luminosities from Gaia parallaxes. Frequency peaks extracted from TESS photometry were used to compar...

  • Article
  • Open Access
13 Citations
6,503 Views
26 Pages

21 July 2022

Six nearly identical versions of an ancient printed book, The Song of Enlightenment with Commentaries by Buddhist Monk Nammyeong Cheon (南明泉和尙頌證道歌), have been found in Korea since the 1920...

  • Article
  • Open Access
17 Citations
7,449 Views
12 Pages

22 March 2016

Epipolar resampling is the procedure of eliminating vertical disparity between stereo images. Due to its importance, many methods have been developed in the computer vision and photogrammetry field. However, we argue that epipolar resampling of image...

  • Article
  • Open Access
18 Citations
5,144 Views
14 Pages

10 January 2023

A novel Babesia sp. infecting goats was discovered based on the molecular findings obtained in the current study, which was conducted in the Mediterranean region of Türkiye. The goal of this study was to isolate this species of Babesia (Babesia...

  • Article
  • Open Access
18 Citations
5,821 Views
37 Pages

Mycobacterium avium (M. a.) subsp. paratuberculosis (MAP) is a worldwide-distributed obligate pathogen in ruminants causing Johne’s disease. Due to a lack of complete subtype III genome sequences, there is not yet conclusive information about g...

  • Article
  • Open Access
4 Citations
3,598 Views
16 Pages

Sequence, Structure, and Binding Site Analysis of Kirkiin in Comparison with Ricin and Other Type 2 RIPs

  • Stefania Maiello,
  • Rosario Iglesias,
  • Letizia Polito,
  • Lucía Citores,
  • Massimo Bortolotti,
  • José M. Ferreras and
  • Andrea Bolognesi

3 December 2021

Kirkiin is a new type 2 ribosome-inactivating protein (RIP) purified from the caudex of Adenia kirkii with a cytotoxicity compared to that of stenodactylin. The high toxicity of RIPs from Adenia genus plants makes them interesting tools for biotechno...

  • Article
  • Open Access
7 Citations
2,905 Views
19 Pages

The Complete Chloroplast Genome Sequence of Eupatorium fortunei: Genome Organization and Comparison with Related Species

  • Kan Yan,
  • Juan Ran,
  • Songming Bao,
  • Yimeng Li,
  • Rehmat Islam,
  • Nai Zhang,
  • Wei Zhao,
  • Yanni Ma and
  • Chao Sun

25 December 2022

Eupatorium fortunei Turcz, a perennial herb of the Asteraceae family, is one of the horticultural and medicinal plants used for curing various diseases and is widely distributed in China and other Asian countries. It possesses antibacterial, antimeta...

  • Article
  • Open Access
5 Citations
4,773 Views
28 Pages

2 July 2020

This work aims to analyze the characteristics and importance that design techniques for disassembly assume in the modern design phase of a mechanism. To this end, the study begins by considering a three-dimensional model of a gear motor, taken from t...

  • Article
  • Open Access
10 Citations
4,255 Views
19 Pages

20 December 2019

Ionising radiation (IR) is known to induce a wide variety of lesions in DNA. In this review, we compared three different techniques that examined the DNA sequence preference of IR-induced DNA damage at nucleotide resolution. These three techniques we...

  • Article
  • Open Access
34 Citations
10,048 Views
9 Pages

Authentication of Cordyceps sinensis by DNA Analyses: Comparison of ITS Sequence Analysis and RAPD-Derived Molecular Markers

  • Kelly Y. C. Lam,
  • Gallant K. L. Chan,
  • Gui-Zhong Xin,
  • Hong Xu,
  • Chuen-Fai Ku,
  • Jian-Ping Chen,
  • Ping Yao,
  • Huang-Quan Lin,
  • Tina T. X. Dong and
  • Karl W. K. Tsim

15 December 2015

Cordyceps sinensis is an endoparasitic fungus widely used as a tonic and medicinal food in the practice of traditional Chinese medicine (TCM). In historical usage, Cordyceps specifically is referring to the species of C. sinensis. However, a number o...

  • Article
  • Open Access
4 Citations
2,806 Views
27 Pages

Lithium-, Phosphorus-, and Fluorine-Rich Intrusions and the Phosphate Sequence at Segura (Portugal): A Comparison with Other Hyper-Differentiated Magmas

  • Michel Cathelineau,
  • Marie-Christine Boiron,
  • Andreï Lecomte,
  • Ivo Martins,
  • Ícaro Dias da Silva and
  • Antonio Mateus

8 March 2024

Near the Segura pluton, hyper-differentiated magmas enriched in F, P, and Li migrated through shallowly dipping fractures, which were sub-perpendicular to the schistosity of the host Neoproterozoic to Lower Cambrian metasedimentary series, to form tw...

  • Article
  • Open Access
3 Citations
1,936 Views
16 Pages

13 October 2024

Malanin is a new type II ribosome-inactivating protein (RIP) purified from Malania oleifera, a rare, endangered tree is only found in the southwest of Guangxi Province and the southeast of Yunnan Province, China. The gene coding sequence of malanin w...

  • Article
  • Open Access
13 Citations
2,857 Views
9 Pages

Comparison of Hepatitis E Virus Sequences from Humans and Swine, The Netherlands, 1998–2015

  • Boris M. Hogema,
  • Renate W. Hakze-van der Honing,
  • Michel Molier,
  • Hans L. Zaaijer and
  • Wim H. M. van der Poel

29 June 2021

Pigs are suspected to be a major source of zoonotic hepatitis E virus (HEV) infection in industrialized countries, but the transmission route(s) from pigs to humans are ill-defined. Sequence comparison of HEV isolates from pigs with those from blood...

  • Article
  • Open Access
3 Citations
1,374 Views
18 Pages

10 January 2025

The shift fork shaft is a key component in transmissions, connecting the shift fork in order to adjust the gear engagement. This study investigates the effects of different welding sequences on deformation and residual stress during plasma welding of...

  • Article
  • Open Access
1,825 Views
20 Pages

The Effect of the I-Arch on the Buccal Alveolar Crest in Comparison with the Traditional Archwire Sequence: A Randomized Controlled Clinical Trial

  • Salam Mouhamad Omar Nakawah,
  • Mohamed Hasan Youssef,
  • Ornella Rossi,
  • Giovanna Perrotti and
  • Tiziano Testori

6 February 2025

Background/Objectives: The nature, diameter, and cross-section of orthodontic archwires affect tooth movement and the surrounding alveolar bone. Researchers have explored different features of archwires to optimize treatment outcomes. In this context...

  • Article
  • Open Access
9 Citations
4,350 Views
15 Pages

Comparison of Extracellular Vesicle Isolation Methods for miRNA Sequencing

  • Meritxell Llorens-Revull,
  • Brenda Martínez-González,
  • Josep Quer,
  • Juan Ignacio Esteban,
  • Gonzalo Núñez-Moreno,
  • Pablo Mínguez,
  • Idoia Burgui,
  • Ricardo Ramos-Ruíz,
  • María Eugenia Soria and
  • Maria Isabel Costafreda
  • + 13 authors

MicroRNAs (miRNAs) encapsulated in extracellular vesicles (EVs) are potential diagnostic and prognostic biomarkers. However, discrepancies in miRNA patterns and their validation are still frequent due to differences in sample origin, EV isolation, an...

  • Article
  • Open Access
9 Citations
6,633 Views
17 Pages

Selecting 16S rRNA Primers for Microbiome Analysis in a Host–Microbe System: The Case of the Jellyfish Rhopilema nomadica

  • Noga Barak,
  • Eduard Fadeev,
  • Vera Brekhman,
  • Dikla Aharonovich,
  • Tamar Lotan and
  • Daniel Sher

Amplicon sequencing of the 16S rRNA gene is extensively used to characterize bacterial communities, including those living in association with eukaryotic hosts. Deciding which region of the 16S rRNA gene to analyze and selecting the appropriate PCR p...

  • Article
  • Open Access
9 Citations
3,809 Views
16 Pages

Protein engineering is actively pursued in industrial and laboratory settings for high thermostability. Among the many protein engineering methods, rational design by bioinformatics provides theoretical guidance without time-consuming experimental sc...

  • Article
  • Open Access
8 Citations
6,090 Views
20 Pages

10 March 2021

Linked-read sequencing was developed to aid the detection of large structural variants (SVs) from short-read sequencing efforts. We performed a systematic evaluation to determine if linked-read exome sequencing provides more comprehensive and clinica...

  • Article
  • Open Access
17 Citations
3,839 Views
16 Pages

Complete Genome Sequencing of Tick-Borne Encephalitis Virus Directly from Clinical Samples: Comparison of Shotgun Metagenomic and Targeted Amplicon-Based Sequencing

  • Samo Zakotnik,
  • Nataša Knap,
  • Petra Bogovič,
  • Tomaž Mark Zorec,
  • Mario Poljak,
  • Franc Strle,
  • Tatjana Avšič-Županc and
  • Miša Korva

10 June 2022

The clinical presentation of tick-borne encephalitis virus (TBEV) infection varies from asymptomatic to severe meningoencephalitis or meningoencephalomyelitis. The TBEV subtype has been suggested as one of the most important risk factors for disease...

  • Article
  • Open Access
41 Citations
5,352 Views
13 Pages

Multi-Laboratory Comparison of Next-Generation to Sanger-Based Sequencing for HIV-1 Drug Resistance Genotyping

  • Neil T. Parkin,
  • Santiago Avila-Rios,
  • David F. Bibby,
  • Chanson J. Brumme,
  • Susan H. Eshleman,
  • P. Richard Harrigan,
  • Mark Howison,
  • Gillian Hunt,
  • Hezhao Ji and
  • Cheryl Jennings
  • + 12 authors

27 June 2020

Next-generation sequencing (NGS) is increasingly used for HIV-1 drug resistance genotyping. NGS methods have the potential for a more sensitive detection of low-abundance variants (LAV) compared to standard Sanger sequencing (SS) methods. A standardi...

  • Article
  • Open Access
2,488 Views
17 Pages

Free Volatile Compounds as Chemophenetic Markers—Comparison with ITS2 and ITS1-5.8S-ITS2 Sequence Data for 18 Species of the Genus Veronica

  • Marija Nazlić,
  • Dario Kremer,
  • Željana Fredotović,
  • Željka Trumbić,
  • Valerija Dunkić and
  • Jasna Puizina

(1) Background: The purpose of this study was to compare the free volatile compounds of 18 Veronica species (Plantaginaceae), as previously analyzed by gas chromatography coupled with mass spectrometry, with their DNA sequences for internal transcrib...

  • Article
  • Open Access
81 Citations
13,586 Views
13 Pages

A Comparison of Whole Genome Sequencing of SARS-CoV-2 Using Amplicon-Based Sequencing, Random Hexamers, and Bait Capture

  • Jalees A. Nasir,
  • Robert A. Kozak,
  • Patryk Aftanas,
  • Amogelang R. Raphenya,
  • Kendrick M. Smith,
  • Finlay Maguire,
  • Hassaan Maan,
  • Muhannad Alruwaili,
  • Arinjay Banerjee and
  • Samira Mubareka
  • + 7 authors

15 August 2020

Genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is increasingly important to monitor the transmission and adaptive evolution of the virus. The accessibility of high-throughput methods and polymerase chain reaction (P...

  • Article
  • Open Access
7 Citations
4,309 Views
27 Pages

The T Cell Receptor (TRB) Locus in Tursiops truncatus: From Sequence to Structure of the Alpha/Beta Heterodimer in the Human/Dolphin Comparison

  • Giovanna Linguiti,
  • Sofia Kossida,
  • Ciro Leonardo Pierri,
  • Joumana Jabado-Michaloud,
  • Geraldine Folch,
  • Serafina Massari,
  • Marie-Paule Lefranc,
  • Salvatrice Ciccarese and
  • Rachele Antonacci

14 April 2021

The bottlenose dolphin (Tursiops truncatus) belongs to the Cetartiodactyla and, similarly to other cetaceans, represents the most successful mammalian colonization of the aquatic environment. Here we report a genomic, evolutionary, and expression stu...

  • Article
  • Open Access
7 Citations
3,548 Views
17 Pages

Molecular and Structure–Properties Comparison of an Anionically Synthesized Diblock Copolymer of the PS-b-PI Sequence and Its Hydrogenated or Sulfonated Derivatives

  • Nikolaos Politakos,
  • Ioannis Moutsios,
  • Gkreti-Maria Manesi,
  • Konstantinos Artopoiadis,
  • Konstantina Tsitoni,
  • Dimitrios Moschovas,
  • Alexey A. Piryazev,
  • Denis S. Kotlyarskiy,
  • Galder Kortaberria and
  • Apostolos Avgeropoulos
  • + 1 author

28 November 2021

An approach to obtaining various nanostructures utilizing a well-studied polystyrene-b-poly(isoprene) or PS-b-PI diblock copolymer system through chemical modification reactions is reported. The complete hydrogenation and partial sulfonation to the s...

  • Article
  • Open Access
1 Citations
5,509 Views
19 Pages

29 August 2022

The research aims to compare the use of 3D materials and video prompting presentations in terms of their effectiveness and efficiency in implementing the CRA teaching strategy. Four students with developmental retardation, aged 8 to 10, participated...

  • Article
  • Open Access
18 Citations
3,366 Views
22 Pages

13 April 2023

From December 2020 to February 2022, 66 lava fountains (LF) occurred at Etna volcano (Italy). Despite their short duration (an average of about two hours), they produced a strong impact on human life, environment, and air traffic. In this work, the m...

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