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209 Results Found

  • Article
  • Open Access
167 Citations
20,150 Views
22 Pages

Targeting the 16S rRNA Gene for Bacterial Identification in Complex Mixed Samples: Comparative Evaluation of Second (Illumina) and Third (Oxford Nanopore Technologies) Generation Sequencing Technologies

  • Raf Winand,
  • Bert Bogaerts,
  • Stefan Hoffman,
  • Loïc Lefevre,
  • Maud Delvoye,
  • Julien Van Braekel,
  • Qiang Fu,
  • Nancy HC Roosens,
  • Sigrid CJ De Keersmaecker and
  • Kevin Vanneste

31 December 2019

Rapid, accurate bacterial identification in biological samples is an important task for microbiology laboratories, for which 16S rRNA gene Sanger sequencing of cultured isolates is frequently used. In contrast, next-generation sequencing does not req...

  • Article
  • Open Access
17 Citations
5,074 Views
21 Pages

Identification of miRNAs and Their Targets Involved in Flower and Fruit Development across Domesticated and Wild Capsicum Species

  • Carlos Lopez-Ortiz,
  • Yadira Peña-Garcia,
  • Menuka Bhandari,
  • Venkata Lakshmi Abburi,
  • Purushothaman Natarajan,
  • John Stommel,
  • Padma Nimmakayala and
  • Umesh K. Reddy

MicroRNAs (miRNAs) are regulators of the post-transcription stage of gene activity documented to play central roles in flower and fruit development in model plant species. However, little is known about their roles and differences in domesticated and...

  • Article
  • Open Access
2 Citations
2,586 Views
14 Pages

31 October 2024

The fauna of Lepidoptera in the Mediterranean is still inadequately documented. As a result, even remotely complete DNA barcode libraries (mt. COI (cytochrome c oxidase 1) gene) are lacking in most areas. This proposed gap is being analyzed for the f...

  • Article
  • Open Access
1,453 Views
35 Pages

23 May 2025

An annotated list of liverworts of the Murmansk Region is compiled based on a critical compilation of publications and label data available in the information system CRIS (L.). It includes 210 species, 2 subspecies and 8 varieties, which is 59 specie...

  • Article
  • Open Access
9 Citations
4,361 Views
18 Pages

DNA Barcoding of Trichobilharzia (Trematoda: Schistosomatidae) Species and Their Detection in eDNA Water Samples

  • Nikolaus Helmer,
  • Christoph Hörweg,
  • Helmut Sattmann,
  • Susanne Reier,
  • Nikolaus U. Szucsich,
  • Jana Bulantová and
  • Elisabeth Haring

12 January 2023

We designed and tested species-specific PCR primers to detect Trichobilharzia species via environmental DNA (eDNA) barcoding in selected Austrian water bodies. Tests were performed with eDNA samples from the field as well as with artificial samples f...

  • Feature Paper
  • Article
  • Open Access
8 Citations
3,721 Views
13 Pages

25 October 2021

Based on literature data, unpublished material and the results of the year-round monitoring at selected sites island-wide by the Cyprus Dragonfly Study Group since 2013, we acquired an excellent knowledge of the diversity and status of the Odonata of...

  • Article
  • Open Access
4 Citations
3,018 Views
12 Pages

Genetic Analysis as a Tool to Improve the Monitoring of Stranded Cetaceans in Chile

  • Sebastián Kraft,
  • Francisca Rodríguez,
  • Carlos Olavarría,
  • Elie Poulin and
  • María José Pérez-Álvarez

19 May 2023

Cetacean strandings are a valuable source of information for several studies from species richness to conservation and management. During the examination of strandings, taxonomic and sex identification might be hindered for several reasons. Molecular...

  • Article
  • Open Access
1 Citations
3,081 Views
10 Pages

25 January 2022

The method of pellet group count is commonly used for estimating population trends of ungulates; however, in the case of species of similar body size, the misidentification rate can be high. Our aim was to find a metrical threshold between pellet gro...

  • Article
  • Open Access
29 Citations
4,981 Views
21 Pages

Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress

  • Zujun Yin,
  • Xiulan Han,
  • Yan Li,
  • Junjuan Wang,
  • Delong Wang,
  • Shuai Wang,
  • Xiaoqiong Fu and
  • Wuwei Ye

5 December 2017

Small RNAs play an important role in regulating plant responses to abiotic stress. Depending on the method of salt application, whether sudden or gradual, plants may experience either salt shock or salt stress, respectively. In this study, small RNA...

  • Article
  • Open Access
8 Citations
3,812 Views
13 Pages

Chemical and Genotypic Variations in Aniba rosiodora from the Brazilian Amazon Forest

  • Diana R. Amazonas,
  • Celso Oliveira,
  • Lauro E. S. Barata,
  • Eric J. Tepe,
  • Massuo J. Kato,
  • Rosa H. V. Mourão and
  • Lydia F. Yamaguchi

25 December 2020

Aniba rosiodora has been exploited since the end of the nineteenth century for its essential oil, a valuable ingredient in the perfumery industry. This species occurs mainly in Northern South America, and the morphological similarity among different...

  • Review
  • Open Access
4 Citations
5,298 Views
20 Pages

3 January 2023

Species belonging to the superorder Batoidea have been poorly assessed due to the lack of information on their life history aspects and their limited economic value. This work presents an overview of skates and rays inhabiting the marine Greek waters...

  • Article
  • Open Access
846 Views
15 Pages

A Sea Anemone Once Thought to Be Invasive in Argentina Is Native to the Southern Atlantic Coast

  • Ricardo González-Muñoz,
  • Jeferson Durán-Fuentes,
  • Agustín Garese,
  • Carlos Spano,
  • Humberto Díaz,
  • Sérgio N. Stampar and
  • Fabián H. Acuña

21 October 2025

Non-indigenous species represent a significant threat to marine biodiversity, and accurate taxonomic identification is critical for effective management. This study revisits the long-standing record of the Australian sea anemone Oulactis muscosa in A...

  • Article
  • Open Access
3 Citations
2,986 Views
20 Pages

Echinoids and Crinoids from Terra Nova Bay (Ross Sea) Based on a Reverse Taxonomy Approach

  • Alice Guzzi,
  • Maria Chiara Alvaro,
  • Matteo Cecchetto and
  • Stefano Schiaparelli

21 July 2023

The identification of species present in an ecosystem and the assessment of a faunistic inventory is the first step in any ecological survey and conservation effort. Thanks to technological progress, DNA barcoding has sped up species identification a...

  • Article
  • Open Access
4 Citations
1,542 Views
13 Pages

4 November 2023

It is unreliable to identify marine fishes only by external morphological features. Species misidentification brings great challenges to fishery research, resource monitoring and ecomanagement. Sillago ingenuua is an important part of commercial mari...

  • Article
  • Open Access
3 Citations
1,896 Views
24 Pages

25 September 2024

Plantago (plantains, Plantaginaceae) is a cosmopolitan genus including over 250 species used as functional foods, forage, and traditional medicine. Among them, Plantago lanceolata is commonly used as an ingredient of herbal products, but the close si...

  • Article
  • Open Access
33 Citations
5,097 Views
20 Pages

New Insights into Interspecific Hybridization in Lemna L. Sect. Lemna (Lemnaceae Martinov)

  • Luca Braglia,
  • Diego Breviario,
  • Silvia Gianì,
  • Floriana Gavazzi,
  • Jacopo De Gregori and
  • Laura Morello

15 December 2021

Duckweeds have been increasingly studied in recent years, both as model plants and in view of their potential applications as a new crop in a circular bioeconomy perspective. In order to select species and clones with the desired attributes, the corr...

  • Article
  • Open Access
8 Citations
4,983 Views
20 Pages

11 January 2023

Biodiversity is considered one of the most significant parameters for characterizing aquatic environments. The value of species diversity is taken into account when assessing the ecological state and quality of the ecosystem. However, erroneous taxon...

  • Article
  • Open Access
3,158 Views
19 Pages

Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus Anoscopus with Particular Reference to the Putative British Endemic Anoscopus duffieldi (Hemiptera: Cicadellidae)

  • Joanna Redihough,
  • Isa-Rita M. Russo,
  • Alan J. A. Stewart,
  • Igor Malenovský,
  • Jennifer E. Stockdale,
  • Rosemary J. Moorhouse-Gann,
  • Michael R. Wilson and
  • William O. C. Symondson

13 November 2020

The subfamily Aphrodinae (Hemiptera: Cicadellidae) contains ~33 species in Europe within four genera. Species in two genera in particular, Aphrodes and Anoscopus, have proved to be difficult to distinguish morphologically. Our aim was to determine th...

  • Feature Paper
  • Article
  • Open Access
2 Citations
1,987 Views
15 Pages

Unusual Findings of Human-Associated Four-Nucleated Entamoeba Species in Captive Wild Animals

  • Lorena Esteban-Sánchez,
  • Juan José García-Rodríguez and
  • Francisco Ponce-Gordo

3 January 2025

Several species of amoebae in the genus Entamoeba forming four-nucleated mature cysts are pathogenic, but their identification is challenging due to a lack of distinctive morphological traits. Furthermore, host species cannot reliably be used as a cr...

  • Article
  • Open Access
377 Views
21 Pages

9 December 2025

Acyl-CoA-binding proteins (ACBPs) are essential lipid carrier proteins involved in plant lipid metabolism. However, the systematic identification and expression profiles of the ACBP gene family in citrus species remain poorly understood. Here, Citrus...

  • Article
  • Open Access
21 Citations
5,581 Views
12 Pages

Characterization of Fecal Microbiota with Clinical Specimen Using Long-Read and Short-Read Sequencing Platform

  • Po-Li Wei,
  • Ching-Sheng Hung,
  • Yi-Wei Kao,
  • Ying-Chin Lin,
  • Cheng-Yang Lee,
  • Tzu-Hao Chang,
  • Ben-Chang Shia and
  • Jung-Chun Lin

26 September 2020

Accurate and rapid identification of microbiotic communities using 16S ribosomal (r)RNA sequencing is a critical task for expanding medical and clinical applications. Next-generation sequencing (NGS) is widely considered a practical approach for dire...

  • Article
  • Open Access
4 Citations
2,104 Views
14 Pages

2 April 2024

The traditional taxonomy of freshwater invertebrates is a labor-intensive process requiring extensive knowledge and experience. In addition, this science is largely subjective, which makes its digitalization difficult. However, accurate species attri...

  • Article
  • Open Access
4 Citations
2,533 Views
19 Pages

29 September 2024

Plant extracellular vesicles are non-self-replicating particles released by living plant cells and delimited by a lipid bilayer. They contain a large amount of lipids, RNA, and proteins. Seed vigor plays an important role in agricultural production a...

  • Article
  • Open Access
1,571 Views
27 Pages

Well-Known, Misidentified, or Unnamed? A DNA Barcode-Based Reassessment of the Lepidoptera Fauna of Cyprus Supported by Morphology

  • Peter Huemer,
  • Özge Özden,
  • Erwin Rennwald,
  • Ian Barton,
  • Jari Junnilainen,
  • Axel Hausmann,
  • Erik J. van Nieukerken and
  • Paul D. N. Hebert

19 December 2025

This study presents the first comprehensive molecular analysis of the Lepidoptera fauna of Cyprus based on DNA barcoding. A total of 1859 DNA barcode sequences were generated, representing 701 Barcode Index Numbers (BINs) and thus putative species. M...

  • Brief Report
  • Open Access
1,187 Views
12 Pages

Development of a Simple and Accurate Molecular Protocol Using 16SrRNA for Species-Specific Identification of Achromobacter spp.

  • Giulia Maria Saitta,
  • Laura Veschetti,
  • Rebecca Feletti,
  • Angela Sandri,
  • Marzia Boaretti,
  • Paola Melotti,
  • Maria Carelli,
  • Maria M. Lleò,
  • Giovanni Malerba and
  • Caterina Signoretto

The Achromobacter genus comprises 22 species and various genogroups. Some species with higher virulence or antibiotic resistance are more likely to cause chronic infections in people with cystic fibrosis (CF). Current identification methods often fai...

  • Article
  • Open Access
5 Citations
2,447 Views
19 Pages

The Binding of Pseudomonas aeruginosa to Cystic Fibrosis Bronchial Epithelial Model Cells Alters the Composition of the Exosomes They Produce Compared to Healthy Control Cells

  • Víctor Lozano-Iturbe,
  • Noelia Blanco-Agudín,
  • Emma Vázquez-Espinosa,
  • Iván Fernández-Vega,
  • Jesús Merayo-Lloves,
  • Fernando Vazquez,
  • Rosa M. Girón and
  • Luis M. Quirós

Cystic fibrosis (CF) is a genetic disease that causes dehydration of the surface of the airways, increasing lung infections, most frequently caused by Pseudomonas aeruginosa. Exosomes are nanovesicles released by cells that play an essential role in...

  • Article
  • Open Access
2 Citations
1,874 Views
14 Pages

Study on the Function of SlWRKY80 in Tomato Defense against Meloidogyne incognita

  • Yinxia Chen,
  • Zhize Wang,
  • Weidan Nie,
  • Tingjie Zhao,
  • Yule Dang,
  • Chenghao Feng,
  • Lili Liu,
  • Chaonan Wang and
  • Chong Du

15 August 2024

WRKY transcription factors (TFs) can participate in plant biological stress responses and play important roles. SlWRKY80 was found to be differentially expressed in the Mi-1- and Mi-3-resistant tomato lines by RNA-seq and may serve as a key node for...

  • Article
  • Open Access
416 Views
14 Pages

Palms Beyond the Forests: The Ex Situ Conservation at the Rio de Janeiro Botanical Garden

  • Claudia Maria Ferrari Barbosa,
  • Diego Rafael Gonzaga,
  • Thiago Favares,
  • Claudine Massi Mynssen,
  • Marcus Alberto Nadruz Coelho and
  • Thaís Moreira Hidalgo de Almeida

The Rio de Janeiro Botanical Garden (JBRJ) holds one of the most diverse ex situ palm collections among tropical botanical gardens. This study describes the current composition of the collection, its taxonomic breadth, and its relevance for conservat...

  • Article
  • Open Access
2 Citations
1,863 Views
67 Pages

21 July 2024

The Nearctic aphid genus Drepanaphis Del Guercio, 1909, the largest within the subfamily Drepanosiphinae (Hemiptera: Aphididae), is characterised by distinctive dorsal abdominal tubercles. This study presents a comprehensive taxonomic revision of the...

  • Article
  • Open Access
3 Citations
1,925 Views
17 Pages

23 December 2024

Sandflies, small insects primarily from the Psychodidae family, are commonly found in sandy, tropical, and subtropical regions. Most active during dawn and dusk, female sandflies feed on blood to facilitate egg production. In doing so, they can trans...

  • Article
  • Open Access
3 Citations
4,421 Views
12 Pages

3 September 2020

Wabash Pigtoe, Fusconaia flava, and the related Round Pigtoe, Pleurobema sintoxia, are freshwater mussels (Bivalvia: Unionidae: Pleurobemini) native to the Great Lakes region of North America. Fusconaia flava is considered widespread and relatively c...

  • Article
  • Open Access
5 Citations
2,729 Views
19 Pages

I Like This New Me: Unravelling Population Structure of Mediterranean Electric Rays and Taxonomic Uncertainties within Torpediniformes

  • Riccardo Melis,
  • Simone Di Crescenzo,
  • Alessia Cariani,
  • Alice Ferrari,
  • Valentina Crobe,
  • Andrea Bellodi,
  • Antonello Mulas,
  • Laura Carugati,
  • Elisabetta Coluccia and
  • Rita Cannas
  • + 1 author

13 September 2023

The present study focused on the three species of electric rays known to occur in the Mediterranean Sea: Torpedo torpedo, Torpedo marmorata and Tetronarce nobiliana. Correct identification of specimens is needed to properly assess the impact of fishe...

  • Review
  • Open Access
703 Views
42 Pages

9 December 2025

Plant microRNAs (miRNAs) are endogenous non-coding RNAs (~20–24 nucleotides) that regulate gene expression post-transcriptionally, playing critical roles in plant growth, development, and stress responses. This review systematically examines AI...

  • Article
  • Open Access
2 Citations
3,921 Views
47 Pages

The Lost MIS 11c Mammalian Fauna from Via dell’Impero (Rome, Italy)

  • Maria Rita Palombo,
  • Biagio Giaccio,
  • Lorenzo Monaco,
  • Roberta Martino,
  • Marina Amanatidou and
  • Luca Pandolfi

4 December 2024

This research presents an in-depth analysis of large mammal remains first discovered in 1932 in the archaeological area of ancient Rome, central Italy, during the work for the opening of Via dell’Impero (VFI). This work describes the faunal ass...

  • Article
  • Open Access
2,608 Views
26 Pages

DNA Barcoding Southwestern Atlantic Skates: A 20-Year Effort in Building a Species Identification Library

  • Ezequiel Mabragaña,
  • Valeria Gabbanelli,
  • Florencia Matusevich,
  • Diego Martín Vazquez,
  • Sergio Matías Delpiani,
  • Victoria Malvina Lenain,
  • Juan José Rosso,
  • Mariano González-Castro,
  • Robert Hanner and
  • Juan Martín Díaz de Astarloa

25 April 2025

The skate fauna in the Southwest Atlantic Ocean (SWA; 34–55° S) is represented by ~32 species, many of which share external features that have led to misidentifications and deficient fishery statistics. The use of DNA barcoding to discrimin...

  • Article
  • Open Access
7 Citations
3,601 Views
29 Pages

Diversity of Arbuscular Mycorrhizal Fungi in Distinct Ecosystems of the North Caucasus, a Temperate Biodiversity Hotspot

  • Andrey P. Yurkov,
  • Alexey A. Kryukov,
  • Anastasiia O. Gorbunova,
  • Tatyana R. Kudriashova,
  • Anastasia I. Kovalchuk,
  • Anastasia I. Gorenkova,
  • Ekaterina M. Bogdanova,
  • Yuri V. Laktionov,
  • Peter M. Zhurbenko and
  • Maria F. Shishova
  • + 5 authors

24 December 2023

Background: Investigations that are focused on arbuscular mycorrhizal fungus (AMF) biodiversity is still limited. The analysis of the AMF taxa in the North Caucasus, a temperate biodiversity hotspot, used to be limited to the genus level. This study...

  • Article
  • Open Access
81 Citations
43,087 Views
17 Pages

Individual Tree-Crown Detection and Species Identification in Heterogeneous Forests Using Aerial RGB Imagery and Deep Learning

  • Mirela Beloiu,
  • Lucca Heinzmann,
  • Nataliia Rehush,
  • Arthur Gessler and
  • Verena C. Griess

6 March 2023

Automatic identification and mapping of tree species is an essential task in forestry and conservation. However, applications that can geolocate individual trees and identify their species in heterogeneous forests on a large scale are lacking. Here,...

  • Communication
  • Open Access
2 Citations
1,754 Views
9 Pages

Development of a Multiplex Polymerase Chain Reaction Method for Rapid and Accurate Identification of Girella punctata and G. leonina (Teleostei: Girellidae)

  • Eun-Mi Kim,
  • Mi-Nan Lee,
  • Chun-Mae Dong,
  • Jae-Koo Noh,
  • Eun-Soo Noh,
  • Woo-Jin Kim,
  • Bo-Hye Nam and
  • Young-Ok Kim

12 August 2023

Girella punctata and Girella leonina are economically important species found in the East Sea; along the southern coast of Korea; south of Hokkaido, Japan; around Taiwan; and in the East China sea. In Korea, these two species hold high value, particu...

  • Article
  • Open Access
1 Citations
1,584 Views
17 Pages

15 May 2024

The distribution of liverworts in the North Pacific is still poorly understood. Scapania umbrosa, which is rare in Asia, was first recorded during the study of the liverwort flora of the Commander Islands (the westernmost Aleutians). The results of t...

  • Article
  • Open Access
15 Citations
4,416 Views
11 Pages

The Issue of Misidentification of Kojic Acid with Flufuran in Aspergillus flavus

  • Marina DellaGreca,
  • Gaetano De Tommaso,
  • Maria Michela Salvatore,
  • Rosario Nicoletti,
  • Andrea Becchimanzi,
  • Mauro Iuliano and
  • Anna Andolfi

In the course of investigations on the complex phenomenon of bee decline, Aspergillus flavus was isolated from the haemocoel of worker bees. Observations on the metabolomic profile of this strain showed kojic acid to be the dominant product in cultur...

  • Article
  • Open Access
12 Citations
4,325 Views
13 Pages

Paper-Based DNA Biosensor for Rapid and Selective Detection of miR-21

  • Alexander Hunt,
  • Sri Ramulu Torati and
  • Gymama Slaughter

8 October 2024

Cancer is the second leading cause of death globally, with 9.7 million fatalities in 2022. While routine screenings are vital for early detection, healthcare disparities persist, highlighting the need for equitable solutions. Recent advancements in c...

  • Article
  • Open Access
7 Citations
3,625 Views
15 Pages

2 January 2023

The leafhopper species, Mukaria splendida Distant, is economically important due to itsstatus as a pest on bamboo and was recently reported to bea vector for phytoplasmal disease. Morphological identification is often difficult and requires a high le...

  • Review
  • Open Access
305 Views
38 Pages

5 January 2026

Cell line misidentification, first exposed when HeLa cells were shown to contaminate dozens of “unique” cultures, now compromises roughly one in five lines and renders thousands of papers potentially unreliable, propagating unreliable dat...

  • Article
  • Open Access
2 Citations
1,275 Views
16 Pages

7 August 2024

Sisyrinchium, a large genus of the Iridaceae family, is divided into ten sections and defined by genetic, morphological and phylogenetic traits. The section Viperella, though monophyletic, encounters taxonomic hurdles, particularly within the Sisyrin...

  • Article
  • Open Access
3 Citations
3,395 Views
14 Pages

20 June 2023

Tremoctopods are epipelagic argonautoid octopods characterized by their expanded dorsal webs and strong sexual size dimorphism, with dwarfed males. The scarcity of taxonomic features attributed to this genus presents a challenge, and there is growing...

  • Article
  • Open Access
9 Citations
4,155 Views
23 Pages

Taxonomy and Melanism Patterns of Freshwater Leeches in the Genus Glossiphonia (Hirudinea: Glossiphoniidae) from Northeast Asia

  • Ivan N. Bolotov,
  • Tatyana A. Eliseeva,
  • Alexander V. Kondakov,
  • Alexander V. Kropotin,
  • Olga V. Aksenova,
  • Yulia V. Bespalaya,
  • Oksana V. Travina,
  • Mikhail Y. Gofarov,
  • Sang Ki Kim and
  • Maxim V. Vinarski
  • + 1 author

8 June 2023

Freshwater leeches belonging to the genus Glossiphonia Johnson, 1816 are fairly common benthic annelids in freshwater ecosystems throughout Eurasia, but the taxonomy of this group remains poorly resolved, and the species content of some local faunas...

  • Article
  • Open Access
1,469 Views
59 Pages

17 November 2025

The aim of this study was to supplement current knowledge on the species diversity of Opostegidae in Central America and the Caribbean and to compare this diversity with that of other regions. We examined historical material and conducted fieldwork i...

  • Article
  • Open Access
64 Citations
11,075 Views
26 Pages

25 March 2015

Strigolactones were described as a new group of phytohormones in 2008 and since then notable large number of their functions has been uncovered, including the regulation of plant growth and development, interactions with other organisms and a plant’...

  • Article
  • Open Access
8 Citations
2,674 Views
15 Pages

8 February 2024

CircRNA, a recently characterized non-coding RNA (ncRNA) variant, functions as a molecular sponge, exerting regulatory control by binding to microRNA (miRNA) and modulating the expression of downstream proteins, either promoting or inhibiting their e...

  • Communication
  • Open Access
1 Citations
2,681 Views
12 Pages

A Standardized Nomenclature Design for Systematic Referencing and Identification of Animal Cellular Material

  • Lisa Schrade,
  • Nancy Mah,
  • Anita Bandrowski,
  • Ying Chen,
  • Johannes Dewender,
  • Sebastian Diecke,
  • Christian Hiepen,
  • Madeline A. Lancaster,
  • Tomas Marques-Bonet and
  • Andreas Kurtz
  • + 9 authors

23 May 2024

The documentation, preservation and rescue of biological diversity increasingly uses living biological samples. Persistent associations between species, biosamples, such as tissues and cell lines, and the accompanying data are indispensable for using...

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