Search Results (37,780)

The biospeckle laser (BSL) technique is recognized as a sensitive method for detecting biological activity and has been successfully applied for seed vigor testing. Given these achievements, whether the integration of BSL into automated systems can provide complementary information on the seed imbibition process remains limited. Addressing this gap represents a significant challenge with strong potential for technological innovation. This study presents an automated laser-optical system designed to monitor the imbibition process of multiple seeds over time using a mechanized carousel. The developed apparatus integrates all necessary components for the illumination and image acquisition of eight seeds across programmable time intervals, controlled by an industrial-grade programmable controller. Validation using maize seeds (Zea mays L.) over a 36-h period confirmed the system’s reliability. BSL indices enabled the characterization of internal biological activity throughout imbibition, revealing dynamic processes that remained undetected in previous discrete-time analyses. These results highlight the potential of the proposed system for more comprehensive and continuous seed monitoring. The successful automated laser-optical system with relative humidity control opens great potential in seeds research and daily industrial analysis. The test of the proposed system in other seeds is the next challenge, regarding the thick and colored coats. The design of larger carousels is a possible step forward, which will demand studies of the limits linked to the illumination and image acquisition time performed in each seed. Full article

The melon fly, Zeugodacus cucurbitae (Coquillett), is recognized as a globally significant quarantine pest, and it ranks among the most destructive insect species infesting cucurbit and solanaceous crops. However, the molecular mechanisms governing reproductive regulation in female Z. cucurbitae remain poorly characterized, particularly those underlying the reproductive processes mediated by microRNAs (miRNAs). In this study, we firstly identified the ovary-specific gene ZcCTL-S1 in Z. cucurbitae via transcriptomic analysis, and subsequently predicted its targeted miRNAs using bioinformatics approaches. Among these miRNAs, overexpression or inhibition of miR-971-1 and miR-let-7 led to corresponding inverse changes in the transcriptional level of ZcCTL-S1. Notably, only miR-let-7 displayed markedly elevated expression levels in Z. cucurbitae ovaries. Further analyses confirmed that miR-let-7 exhibited a direct targeting relationship with ZcCTL-S1, via a combinatorial approach involving in vivo RNA immunoprecipitation, in vitro dual-luciferase reporter assays, and site-directed mutagenesis techniques. Phenotypic analyses showed that both knockdown of ZcCTL-S1 and overexpression of miR-let-7 significantly inhibited egg hatchability, ultimately compromising the female reproductive capacity of Z. cucurbitae. Collectively, these findings identify a novel miRNA-gene regulatory module in the reproductive development of Z. cucurbitae, and provide novel insights for the development of gene- or miRNA-based pest control strategies. Full article

The vaginal microbiome (VM), a complex and dynamic microbial ecosystem, is now recognized as a central determinant of female reproductive and gynecologic health. Under homeostatic conditions, a Lactobacillus-dominant ecosystem maintains vaginal acidity, provides colonization resistance, and modulates mucosal immunity. Conversely, vaginal dysbiosis—characterized by Lactobacillus depletion and anaerobic or aerobic overgrowth—is associated with infectious vaginitis, increased susceptibility to sexually transmitted infections, and non-infectious conditions such as genitourinary syndrome of menopause. This review provides an integrated overview of the composition, functional characteristics, and host interactions of the VM across health and disease. We highlight major mechanisms by which microbial dysbiosis contributes to disease pathogenesis, including biofilm formation, altered microbial metabolism, and immune dysregulation. In addition, we discuss the translational potential of the VM as a source of diagnostic and prognostic biomarkers and as a target for emerging microbiome-dependent therapeutic strategies. Collectively, current evidence supports the view that vaginal dysbiosis is a heterogeneous and context-dependent state driven by distinct pathogen- and host-related mechanisms, underscoring the importance of prioritizing microbiome restoration rather than pathogen eradication alone. Full article

Conventional Fiber-Reinforced Polymer (FRP) materials may exhibit certain performance uncertainties in harsh environments, limiting their reliability for structural strengthening. To address this, Basalt Fiber-Reinforced Polymer (BFRP) plates fabricated with silicate-modified epoxy resin are proposed for the flexural strengthening of reinforced concrete (RC) beams. The research aims to evaluate their short-term strengthening performance and establish a reliable calculation method for flexural capacity. Four-point bending tests were conducted to investigate the effects of BFRP plate thickness and end anchorage configuration on failure modes, flexural capacity, and ductility. Finite element simulations incorporating interfacial bond–slip behavior reproduced typical debonding failures, followed by a comprehensive parametric analysis. Based on the experimental and numerical results, a modified BFRP plate strain formula at debonding was proposed to establish a calculation method for the flexural capacity of BFRP-strengthened beams governed by debonding failure. The results indicate that beams without end anchorage were prone to interfacial debonding, where increasing the plate thickness from 0.5 mm to 2 mm raised the flexural capacity gain from 4.5% to 15% but intensified the ductility reduction from 42.9% to 64.9%. Conversely, applying mechanical anchorage improved the ductility index by over 20% compared to unanchored counterparts. The adopted FRP–concrete bond–slip constitutive model accurately characterizes interfacial debonding behavior, and the proposed flexural capacity model demonstrates high accuracy with overall deviations within 5%. It can be concluded that the novel BFRP plates exhibit strengthening behavior comparable to existing FRP systems. Effective end anchorage further enhances flexural capacity and prevents brittle failure. The proposed debonding strain formula for the novel BFRP system offers a reliable basis for capturing the critical onset of interfacial failure. Building upon this, the developed flexural capacity model provides a reliable theoretical basis for the design and assessment of RC beams strengthened with the novel BFRP plates. Full article

Background: Surgical tendon rupture repair suffers from scar formation, leading to tendons with inferior mechanics and consequently to re-ruptures, as well as from adhesion formation to the surrounding tissue, reducing the range of motion. In an approach of re-purposing the phytochemical Bakuchiol to be incorporated in the polymer DegraPol^® (DP), we fabricated a novel implant material by emulsion electrospinning. Methods: To characterize the emulsion electrospun novel materials, we used Scanning Electron Microscopy (SEM) to determine the fiber diameter and pore size. In addition, we used Fourier Transformed Infrared Spectroscopy (FTIR). Finally, we planted the materials onto the chorioallantoic membrane of the chicken embryo (CAM assay) to assess tissue integration and collagen expression. Results: While the pure DP meshes were very well integrated in the CAM assay and showed a significantly higher collagen deposition within the scaffold, the DP + Bakuchiol meshes exhibited poor tissue integration, showing rather the beginning of a fibrous encapsulation. Conclusions: The novel electrospun material DP + Bakuchiol could be used as an anti-adhesion barrier to prevent tendon adhesion. Full article

The geometric variability of industrial components represents a persistent challenge in robotic arc welding, particularly in high-volume manufacturing environments where parts are positioned in fixtures based on nominal CAD assumptions. Even moderate deviations in dimensions or seating conditions can lead to weld defects, rework, and reduced process capability when conventional offline programming is employed. This paper presents an applied industrial workflow for adaptive robotic welding trajectory correction that integrates full-field 3D optical metrology with a data-driven deep reinforcement learning (DRL) model. Prior to welding, each component is scanned using a structured-light 3D system, and critical geometric deviations are extracted relative to the nominal CAD model. These deviations define a compact state representation that is mapped, via a trained DRL agent, to corrective translational and rotational adjustments of the welding trajectory. Importantly, all trajectory corrections are computed offline, ensuring compatibility with standard industrial robot controllers and avoiding real-time computational overheads. The proposed approach is validated using real production data from an industrial batch of 5000 components characterized by significant dimensional variability and limited process capability. Experimental results demonstrate a reduction in welding defects exceeding 90%, elimination of rework associated with improper part positioning, and an improvement of the overall process performance to a sigma level of 5.219. The results show that combining 3D optical metrology with learning-based trajectory adaptation enables robust compensation of part-level geometric deviations without mechanical fixture modifications. The proposed method provides a practical and scalable solution for improving welding quality in manufacturing environments affected by upstream variability and imperfect part positioning. Full article

Carbapenems are essential for the treatment of severe infections caused by Gram-negative bacteria, particularly in critically ill and immunocompromised patients. However, the global rise of carbapenem-resistant Enterobacterales (CRE), Pseudomonas aeruginosa, and Acinetobacter baumannii has significantly eroded their effectiveness, and the phenomenon is now recognized as a major public health threat. Resistance is driven by the complex and evolving interplay of enzymatic and non-enzymatic mechanisms, occurring within highly successful clonal lineages and mobile genetic platforms. This review summarizes advances since 2020 in the molecular basis of carbapenem resistance, integrating enzymatic mechanisms across Ambler classes A, B, C, and D with emerging non-enzymatic contributors, including porin remodeling, efflux pump upregulation, target-site alterations, and outer-membrane adaptations. Particular attention is given to adaptive genome dynamics, such as IS26-mediated gene amplification, plasmid multimerization, and heteroresistance, that generate unstable resistance phenotypes and complicate routine susceptibility testing. Newly introduced β-lactam/β-lactamase inhibitor combinations exert distinct selective pressures: ceftazidime–avibactam favors KPC Ω-loop variants and permeability defects, often restoring carbapenem susceptibility, whereas meropenem–vaborbactam and imipenem–relebactam resistance is driven mainly by porin loss and β-lactamase gene amplification. Cefiderocol resistance is multifactorial, frequently involving impaired siderophore uptake and heteroresistance, while sulbactam–durlobactam remains active against OXA-producing A. baumannii but is compromised by metallo-β-lactamases and PBP3 alterations. Carbapenem resistance is increasingly characterized by convergent, multi-layered adaptations that undermine both established and novel therapies. While high-level randomized evidence remains limited for some resistance mechanisms, emerging mechanistic, microbiological, and clinical data support the need for mechanism-aware diagnostics, repeated susceptibility assessment during therapy, and stewardship strategies informed by resistance biology. Integrating molecular context into routine practice will be critical to preserving emerging treatment options and limiting the global impact of carbapenem resistance. Full article

Waste lignin from the hydrolysis of lignocellulosic materials is an abundant but underused by-product of the pulp and biorefinery industries. Phenolic compounds derived from lignin, rich in aromatic structures, show strong antioxidant potential and can be applied in polymer stabilization, food, and medical fields. This study evaluated the radical-scavenging activity of phenolic fractions obtained from alkaline-treated waste lignin against DPPH● and ABTS•⁺, using Trolox as a reference. Both spectrophotometric and electrochemical techniques were employed, providing deeper insight into the underlying mechanisms. Depending on the assay, the phenolic extracts demonstrated substantial radical-scavenging capacity, in some cases matching or surpassing that of Trolox. This behavior was linked to electron/proton transfer pathways, radical reactivity, and solubility effects. The combined use of multiple antioxidant tests offered a comprehensive characterization of the bioactivity of lignin-derived phenolics and supports their potential as sustainable sources of antioxidant compounds within a circular economy framework. Furthermore, the study examined how toluene-extracted phenolics affect the thermo-oxidative stability of model polyurethane films. Incorporating small amounts (1%, 3%, 5%) into the polymer matrix showed that a 1% loading provides the most effective stabilization. At higher concentrations, however, additional oxidative processes seem to be activated, as indicated by FTIR measurements and thermogravimetric analysis. Full article

Phosphorus deficiency is a key factor limiting plant growth in desertified grasslands. Elucidating the adaptive strategies of pioneer plants that integrate root morphology and microbial interactions is crucial for understanding the natural restoration of ecosystems. This study investigated the strategies employed by Kengyilia hirsuta, a pioneer grass species in desertified grasslands, to adapt to low-phosphorus environments. By conducting sand culture experiments under varying phosphorus levels (low, optimal, and high), we focused on elucidating the synergistic adaptive mechanisms involving the root–rhizosheath system. The results showed that the rhizosheath serves as a critical micro-ecological niche for enriching arbuscular mycorrhizal fungi (AMF) and enhancing phosphatase activity. Under low-phosphorus stress, the plant strengthened root hair development and its symbiotic association with AMF, which markedly increased acid phosphatase activity and led to the highest phosphorus use efficiency. At the optimal phosphorus level, the plant developed an efficient “rhizosheath–mycorrhiza” synergistic system, characterized by high AMF colonization and spore density, facilitating optimized carbon–phosphorus exchange. Under phosphorus-sufficient conditions, the adaptive strategy transitioned towards root morphological plasticity, exemplified by increased surface area and branching. Multivariate analysis revealed that the phosphorus absorption efficiency of K. hirsuta is co-regulated by both morphological adaptation and symbiotic optimization. This study elucidates the mechanisms of nutrient stress adaptation in desertified grassland plants, providing a theoretical foundation for understanding the natural restoration processes of degraded ecosystems. Full article

Strike-slip faults and their associated fractures in the ultra-deep marine carbonate reservoirs of the Fuman Oilfield, Tarim Basin, hold significant petroleum geological importance, with the developmental characteristics of fractures being a key factor controlling reservoir productivity. This study targets the F_I17 strike-slip fault zone within the oilfield, where a comprehensive evaluation of fracture effectiveness was performed by integrating geological methods, including core and thin section observation, fluid inclusion thermometry, geophysical fracture identification approaches using imaging logging and seismic data, and geomechanical simulations. The results showed that: (1) structural fractures were developed in at least three stages, predominantly high-angle fractures with their strikes obliquely intersecting the main fault at a small angle, and were affected by multiple episodes of fluid activity, while early-phase fractures exhibited severe filling whereas late-phase fractures had good effectiveness; (2) ultra-deep carbonate rocks contained well-developed stylolites, with extensive horizontal stylolites reducing fracture effectiveness; (3) mechanical effectiveness evaluation parameters were proposed by integrating normal stress, shear stress, and formation pressure, with slip tendency as the dominant indicator, and referenced to the leakage factor and dilation tendency to characterize fracture effectiveness; (4) dynamic effectiveness was assessed using closure/opening pressures, defining a reasonable formation pressure range for hydrocarbon exploitation. The findings of this study can provide theoretical guidance for the further exploration and development of ultra-deep reservoirs in the Fuman Oilfield. Full article

Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus (family Geminiviridae) originally isolated from tomatoes and later evolved to cross-infect cucurbit crops, causing severe economic damage in Asia and Europe. In this study, we sequenced and characterized complete genomes of two ToLCNDV isolates collected from Hebei (ToLCNDV-HB) and Jiangsu (ToLCNDV-JS) provinces of China infecting melon. We constructed infectious clones for ToLCNDV-HB and ToLCNDV-JS, which could systemically infect Nicotiana benthamiana, tomato, and four species of cucurbitaceous plants. Notably, ToLCNDV-HB induced more severe symptoms and accumulated higher viral DNA and protein accumulation than ToLCNDV-JS in N. benthamiana, melon, and bottle gourd. Sequence analysis showed that sequence variations are present only in AV2, AC1, and AC4. However, only the AV2 ORF from ToLCNDV-HB was more efficient than that from that ToLCNDV-JS in enhancing potato X virus’s pathogenicity and suppressing post-transcriptional gene silencing (PTGS). An AV2-swapping experiment between ToLCNDV-HB and ToLCNDV-JS confirmed its vital role in determining the differential pathogenicity. Further evidence shows that virions from both clones are mechanically transmissible. This is the first report comparing the differential pathogenicity of two Chinese ToLCNDV isolates in cucurbits. The AV2 protein, a key pathogenicity determinant, represents a potential target for breeding ToLCNDV-resistant cucurbit varieties. Full article

Catechins are characterized by a basic structure consisting of two benzene rings and a hydropyran heterocyclic ring. In (-)-epicatechin (ECT), the substituents in C2 and C3 of the dihydropyran ring are in cis conformation, whereas in (+)-catechin (CT), they are in trans conformation. Catechins tend to interact with membrane proteins, affecting their activity and/or function and metabolic processes. In this study, the impact of CT and ECT on erythrocyte membrane and cell functions was analyzed. Surprisingly, although the two compounds have a very similar structure that differs only in the orientation of the hydroxyl group in C3, they promote different effects on anion exchange through the phospholipid bilayer and on the release of ATP from cells. Anion transport mediated by Band 3 protein is reduced in the presence of CT compared with ECT which conversely increases it, and this observation aligns with the mechanisms of action we hypothesized in silico for the two compounds. Finally, ECT causes an increase in intracellular ATP levels unlike CT, and both molecules cause a decrease in ATP released from the erythrocyte. These findings could pave the way for further studies aimed at better understanding of the potential properties and structure–activity relationships of these molecules. Full article

Notch wear in austenitic stainless steel turning develops rapidly and remains a key productivity limitation with carbide tools. This work identifies the initiation mechanism of notch wear when turning EN 1.4307 stainless steel using CVD-coated cemented carbide inserts with an Al₂O₃ top layer. Turning tests were performed under dry conditions, followed by optical wear measurements and chip surface analysis. The tool–chip interface chemistry and material transfer were characterized using SEM/EDS, while high-frequency acoustic emissions were recorded to resolve the dynamics of adhesive events. Thermo-mechanical FEM simulations were conducted to map contact pressure and temperature along the cutting edge. The results show that adhesive wear initiates immediately at engagement and governs notch formation: polluted SiO₂ deposits act as an active bonding medium, and repeated bond formation/rupture removes extremely thin flakes of tool and coating material, evidenced by Al₂O₃ and Ti(C,N) fragments on the chip and by characteristic acoustic cluster waves. A new tool–chip contact model is presented, indicating that high pressure and high temperature within the polluted SiO₂ near the chip’s outmost side promote larger, stronger adhesive bonds together with the absence of ceramic particles near the rake in the notch area. Oxidation and diffusion are assumed to be secondary processes that become relevant after local coating loss, while adhesion remains the primary removal mechanism during early and intermediate stages. Full article

CD8⁺ memory T (T_M) cells are essential for vaccine-induced protective immunity. While transforming growth factor beta (TGF-β) triggers CD8⁺ T_M cell differentiation, the underlying molecular mechanism(s) has yet to be uncovered. We therefore used a well-established cell culture protocol to prepare TGF-β-triggered CD8⁺ T_M cells derived from chicken ovalbumin (OVA)-specific T cell receptor (TCR) transgenic OTI mice, and systematically characterized them using Western blotting, confocal microscopy, flow cytometry and Seahorse assay analyses. We found that TGF-β/T cells exhibit a T_M cell phenotype (CD62L⁺KLRG1⁻) and display long-term survival upon adoptive transfer into mice. To elucidate the signaling circuitry underpinning the observed transcriptional and metabolic changes required to promote CD8⁺ T_M cell differentiation, we measured the expression of several critical factors and found that TGF-β triggered weak mTORC1 (mTORC1^Weak) signaling. mTORC1^Weak signaling in turn led to an increase in the abundance of key transcriptional (TCF1, FOXO1 and Eomes) and metabolic (AMPK-α1, ATG7, ULK1, SIRT1, OPA1 and LAL) factors and an elevation in mitochondrial mass and reliance on fatty acid oxidation (FAO). Our data thus reveal for the first time that TGF-β regulates CD8⁺ T cell memory by triggering mTORC1^Weak-mediated activation of the transcriptional FOXO1-TCF1-Eomes and metabolic AMPK-ULK1-ATG7 pathways. Given that induction of more qualified CD8⁺ T_M cells is one of the ultimate goals of vaccination, our findings identify additional targets critical to TGF-β-induced T cell memory, which may greatly impact future vaccine development for the treatment of cancer and infectious diseases. Full article

A high strength Al-12Si has been prepared through mechanical alloying and press forming without any additional alloy components. The alloy exhibited a high tensile strength of 458 MPa at room temperature and retained excellent tensile properties at elevated temperatures with a UTS of 118 MPa at 350 °C after 1000 h of exposure. Furthermore, after 1000 h of heat exposure testing, the mechanical properties of the alloy showed no significant decrease. X-ray diffraction characterizations indicated that the alloy consists solely of an Al matrix and Si phase. Microstructural characterization through HRTEM revealed that the grain size of the Al matrix was approximately 300 nm, with a high-density of stacking faults present. The grain refinement strengthening and stacking fault strengthening contributed to the alloy’s excellent mechanical properties at both room temperature and elevated temperatures. Full article